LOCUS       NM_027491               5047 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Ras-related GTP binding D (Rragd), transcript variant
            2, mRNA.
ACCESSION   NM_027491
VERSION     NM_027491.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Zhang,Y., Hu,H., Liu,W., Yan,S.M., Li,Y., Tan,L., Chen,Y., Liu,J.,
            Peng,Z., Yuan,Y., Huang,W., Yu,F., He,X., Li,B. and Zhang,H.
  TITLE     Amino acids and RagD potentiate mTORC1 activation in CD8+ T cells
            to confer antitumor immunity
  JOURNAL   J Immunother Cancer 9 (4) (2021)
   PUBMED   33883257
  REMARK    GeneRIF: Amino acids and RagD potentiate mTORC1 activation in
            CD8(+) T cells to confer antitumor immunity.
REFERENCE   3  (bases 1 to 5047)
  AUTHORS   Srivastava,A.K., Kapur,S., Mohan,S., Yu,H., Kapur,S., Wergedal,J.
            and Baylink,D.J.
  TITLE     Identification of novel genetic loci for bone size and
            mechanosensitivity in an ENU mutant exhibiting decreased bone size
  JOURNAL   J Bone Miner Res 20 (6), 1041-1050 (2005)
   PUBMED   15883645
REFERENCE   4  (bases 1 to 5047)
  AUTHORS   Takasato,M., Osafune,K., Matsumoto,Y., Kataoka,Y., Yoshida,N.,
            Meguro,H., Aburatani,H., Asashima,M. and Nishinakamura,R.
  TITLE     Identification of kidney mesenchymal genes by a combination of
            microarray analysis and Sall1-GFP knockin mice
  JOURNAL   Mech Dev 121 (6), 547-557 (2004)
   PUBMED   15172686
REFERENCE   5  (bases 1 to 5047)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   6  (bases 1 to 5047)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772288.12, CK387674.1 and BC038137.1.
            
            On Dec 30, 2006 this sequence version replaced NM_027491.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-421               AL772288.12        67296-67716
            422-897             CK387674.1         1-476
            898-1815            BC038137.1         387-1304
            1816-5047           AL772288.12        103247-106478
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 14.57 cM"
     gene            1..5047
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /note="Ras-related GTP binding D"
                     /db_xref="GeneID:52187"
                     /db_xref="MGI:MGI:1098604"
     exon            1..610
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     CDS             466..1665
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ras-related GTP-binding protein D; ragD; rag D"
                     /codon_start=1
                     /product="ras-related GTP-binding protein D isoform 2"
                     /protein_id="NP_081767.2"
                     /db_xref="CCDS:CCDS38704.1"
                     /db_xref="GeneID:52187"
                     /db_xref="MGI:MGI:1098604"
                     /translation="MSQVLGKPQPQGEDGGEDEEEDELVGLAGYEDGPESSDAELDSG
                     PEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPSETLFLESTNRICRE
                     DVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLT
                     VTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLT
                     SIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTP
                     VDMQTYELCCDMIDVVIDISCIYGLKEDGAGAPYDKDSTAIIKLNNTTVLYLKEVTKF
                     LALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKMVKSRKAQSRLPKKTGATPN
                     GTPRVLL"
     exon            611..906
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1106
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1221
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1364
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1513
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..5047
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5024..5029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /note="hexamer: AATAAA"
     polyA_site      5047
                     /gene="Rragd"
                     /gene_synonym="5730543C08Rik; C030003H22Rik; D4Ertd174e"
                     /note="major polyA site"
ORIGIN      
        1 agaaagcctg aagaccgcag cacaagccag gggcactgca gaaagcaggc ggcagggtcc
       61 gggtggtccc cgggccacgc ttgcagagaa agcgagcaga agtccccgag ggcggtggcc
      121 agtgcgctct ggacacccga attctctcta gggggagaac gtgtgtggcc gagcaagcgg
      181 tggctgcggc cggcgagcgc gcgcccggtg gtgctgcgtg tgtgtgtgtg tgtgtgtgtg
      241 tgtgtgtgtg tgtgtgtgtg tgtgtgacaa gcgtcaggtg actcgggtca cgcagtcact
      301 ccctcgcgct cccggggaga actgccgcgc tccggctggc ttctcgggca gcagcggcag
      361 ggagggggct gggctccact gccaccccct tgctcaccag gcgcggcccc agggcgctgc
      421 gggccactcg gcccacgcgg gaccttggag gcaagcgtgg gcgtgatgag ccaagtgctg
      481 ggcaagccgc agccgcaggg tgaggacggc ggcgaggacg aggaggagga tgagctggtg
      541 gggctggcgg gctacgagga tgggcccgag tcctcggacg ccgagctgga cagcgggccg
      601 gaggagggag ttctggactt cagcgacccc ttcagcactg aggtgaagcc cagaatcctg
      661 ctcatgggtc tcaggaggag cggcaagtcg tctatccaga aagtcgtctt tcacaaaatg
      721 tccccgagcg agacgctgtt cttggagagc accaacagga tctgccggga agacgtctcc
      781 aacagctcct tcgtcaactt ccagatttgg gacttcccag ggcagattga cttttttgac
      841 cctacctttg actatgagat gatcttccgg ggaaccgggg cactgatctt cgtcatcgac
      901 tcccaggatg actatatgga agcgctggcc cggctccatc tcacagtgac cagggcctac
      961 aaggtgaaca ctgatatcaa ctttgaggtg tttattcata aagtagatgg tctgtcagat
     1021 gaccacaaaa ttgaaaccca gagagacatt caccagaggg caaacgatga cctcgctgat
     1081 gctggattag aaaaaattca cctgagcttt tacctgacaa gcatatatga tcattcaata
     1141 tttgaagcct ttagcaaagt ggttcagaaa ctgatcccac agctcccgac gctggagaat
     1201 ctgctgaaca tctttatctc aaattctgga attgagaaag cgtttctgtt tgacgtggtc
     1261 agtaagattt atattgcaac cgacagcacg ccggtggaca tgcagacgta cgagctctgc
     1321 tgtgacatga ttgacgtggt gatcgacatc tcttgtattt atggtctcaa agaagatgga
     1381 gcaggagccc cgtatgacaa ggactcaaca gccattataa agctgaataa tacaacagtt
     1441 ctttatttaa aagaggtcac aaagttcttg gcgctcgttt gcttcgtcag agaggaaagc
     1501 tttgaaagaa aagggctgat tgactataat ttccactgct tccggaaggc catccatgaa
     1561 gtcttcgagg tgaggatgaa gatggtgaaa tctcgaaagg cccagagtcg gctacccaag
     1621 aagacaggag ctactcccaa tgggacccct agagtgctgc tgtaggggaa ctgtcaggag
     1681 cctgagtcca gacctcagtg aggaggctgt taaactatac tgcactacag gagggctggg
     1741 gccctgactc gtatgtgtta agggaaaaaa aaaaacctta ctctttaaaa taaaatgagc
     1801 actttgaagc aaaaacctgt acaataacag tgagagccac tgtactgtca ctacgtaagt
     1861 gtaaactttt atgttgatag aaaaccccct gtgaggactg ccaggaactg tacatatttt
     1921 gcatattttc atgttttttt tcctcattga ggtgaacttt gataagatga cttttctaaa
     1981 ctcttttctg tacttgatga caaggacacg catactctac agtccatatg gcaatcggaa
     2041 attgatccat tatattgcta gagacttttt ttgtaaattt gggactcttt gctgccagtt
     2101 gtcaaagggg aaatcgtttt tcagtggagc agcaacaggt cagggctgga agctccaggc
     2161 cagagcaata agaacgtctc tatttatagg aagtgtaata ggaatgttgt agctggacag
     2221 tggtggtgca tgcctttaat cccagcagat ggatctctgt gagtttgagg tcagcctgat
     2281 cgacagagtg agttccagga cagcgagggc tacacagaga aaccctgtct caaaaccaac
     2341 caaacaaatg ccccccccaa aacaaaacag gaattttgta ggaaatacta ttaccaagct
     2401 tacccacaca tttgtgccct atggcattgc cttccgactc gcccagtctt tatgaaactc
     2461 tttacctcaa cacacctttg cagtcacctt gtgcagacgg gtgatttctt tttggtgcag
     2521 tatcttgtga acctttggga ccagatttct aaaatgatgc caaccagtgt agagaaatat
     2581 ggatgtcgtc tgaggagatt ggaacagata gcattgattt gtgtgggggg gaaaccgtag
     2641 aagcaatact gtacctgccg aggggtctaa aacacacaag ccaagagggt tcccagatgt
     2701 aggcagaggt cactcagata agagatgcac gtgtgcttgt gtgtgttcac ctcctgggaa
     2761 tggtgtgttg atgcgctatg ttcagagacc ttgttcagga ccaggtttta atgctccatg
     2821 accacaggag tttagtcctt ggtggctcca tttgttacct gactcgttct ttcatctgta
     2881 acaacatacc tcatgtcctt tgcctgttcc taccaagatc agcttgccac cagcttgctc
     2941 tgcaagacag aagacacaga aagcctgtgg ccggtgggaa aaaaaggagg cctagagacg
     3001 tatgttagtc agttaactat gtgtgtgctt ttatagatag gtagggaact aagagtccta
     3061 aatctagccc tatttcaaac cattctaagg gttagtactg tcaggcgtgc atggcattag
     3121 caaaggttct ttttaaagca gagtccctta gggatgtcaa agctggttct agctttgtgt
     3181 ctaaagcagc ttgctcctga agctcacttc ttttacgcat cttcttccca tcttcttgct
     3241 tccttttccc caaattcttt attatttgag acagcgtatc tctatgtagc cctggctgtc
     3301 ctggaactca ctatgtagac cggactgtcc ttgaactcag agattcacct gcctctgctt
     3361 ccccaagtga tcaaaggtga acaccaccat gtctggccag gtcttacact tttggagctg
     3421 gtaactaaaa ctggagacga tgattgaaat gtaagattga gcctcccagt agtctgcaca
     3481 ttattaagga gatttctagt catcatggaa ccatggctcc ttccttatga tttctatgaa
     3541 gagacttgaa tgagatggta ggagcctgga gttctaatct tcaggactat tccccgacgg
     3601 cctctgccaa cagagtggat ggtggttggc cacttttaag agtctaacac cactgtgcac
     3661 ccacacaaag acactagaga gtgagaaaaa gcatagtttt ctctttttga gtggggctca
     3721 gaggtaactc aggctggcct cagacttgcc aggtagctaa ggatgctctc cagtttctga
     3781 ccgctcagcc tcgactttcc ctaagtgcta ggattatagg aatgcaacac cgtgcttggc
     3841 ttgaacagtg ctgagaactg gacccagagc tttgtgcatg tgaaggcaag cactctacca
     3901 atctagtatc cccagcatcc tttcttaata gttttttttt ttttttaaac aaagaatttc
     3961 tctttgaata gacaaagaat taagttttta ataagactgt agtttcaaaa taagacactt
     4021 gcctgtctgg taaatcacat aaaattagca tgtctaagtg gcgcttccat ttttaagctg
     4081 gttatatcat gagtttttgt tgttgaaatg tgccttaaaa gcctatcact tcaagagcat
     4141 gtgattcttt gagggatagg caggaacact cctgtgacat agggcccatg ttcctgatca
     4201 taaatgcact ctttagttac aacactgatt cctgcctcga gcctggtgtg acactgacct
     4261 cttgaagatg tctgaatgtg actagaattg cgaaatgtcc tcacatttgt tttgtatttt
     4321 atcttagatt tggggggtta gcttactgca ctgtagatct gtgtctatgc tcaaatggga
     4381 ctataaactg aaaggtcaaa ctcctaacta cggtggcttc agtagaacat gtgtttggtt
     4441 ttaggaattg agaacttgaa gatgtgtaga gcaggcagac ttttgctctc tgagtcaagt
     4501 acagcttcta tgtaagagtt gtgccgaatt acttgtcata atggaggtta gtgtgagtga
     4561 cctgtacaga gtgcttttga aggagtagaa ataaaagctg attaatagat accacgtgtt
     4621 attttgtgaa cataatatat tttggatcat taaatagaaa atggctaaat atcacagcag
     4681 tactattaag tatgggttat ttgtcaactg agtctgaaac agaatttatc aaatcaatgg
     4741 aactgtcatg attgacgtat ggtatgcact caacaaacac aggctgggtg tagtttgtaa
     4801 tcaagcacac tgttttgctg ttgctaggac ttcatttgta acacagtctt cagggatgtt
     4861 aactgccaac ttgaaagcat gtttgtgtgc cgatggcttg acgtaccccg tacccatagc
     4921 ttagttttct gggagcagaa tggtctcttg ttgaaattca agatgatgtt tactatatac
     4981 atatatatac atcactgttt tattggttat gttaggttca tttaataaaa agaagtttta
     5041 aaaataa
//
