LOCUS       NM_019662               1539 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus Ras-related associated with diabetes (Rrad),
            transcript variant 1, mRNA.
ACCESSION   NM_019662
VERSION     NM_019662.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Elmore,G., Ahern,B.M., McVay,N.M., Barker,K.W., Lohano,S.S.,
            Ali,N., Sebastian,A., Andres,D.A., Satin,J. and Levitan,B.M.
  TITLE     The C-terminus of Rad is required for membrane localization and
            L-type calcium channel regulation
  JOURNAL   J Gen Physiol 156 (9) (2024)
   PUBMED   38990175
  REMARK    GeneRIF: The C-terminus of Rad is required for membrane
            localization and L-type calcium channel regulation.
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1539)
  AUTHORS   Ahern,B.M., Sebastian,A., Levitan,B.M., Goh,J., Andres,D.A. and
            Satin,J.
  TITLE     L-type channel inactivation balances the increased peak calcium
            current due to absence of Rad in cardiomyocytes
  JOURNAL   J Gen Physiol 153 (9) (2021)
   PUBMED   34269819
  REMARK    GeneRIF: L-type channel inactivation balances the increased peak
            calcium current due to absence of Rad in cardiomyocytes.
REFERENCE   4  (bases 1 to 1539)
  AUTHORS   Ahern,B.M., Levitan,B.M., Veeranki,S., Shah,M., Ali,N.,
            Sebastian,A., Su,W., Gong,M.C., Li,J., Stelzer,J.E., Andres,D.A.
            and Satin,J.
  TITLE     Myocardial-restricted ablation of the GTPase RAD results in a
            pro-adaptive heart response in mice
  JOURNAL   J Biol Chem 294 (28), 10913-10927 (2019)
   PUBMED   31147441
REFERENCE   5  (bases 1 to 1539)
  AUTHORS   Zhang,P., Bai,H., Li,J., Liu,J., Ma,W., Xu,B., Xia,Q., Wang,J.,
            Fan,Yi. and Du,Q.
  TITLE     Knockdown of slincRAD leads to defective adipose development in
            vivo
  JOURNAL   Biochem Biophys Res Commun 513 (4), 983-989 (2019)
   PUBMED   31005260
  REMARK    GeneRIF: Compared to normal adipocytes, slincRAD knockdown cells
            had defective differentiation features, such as smaller sizes and
            decreased lipid production. For elder mice, slincRAD knockdown led
            to abnormal glucose and lipid metabolism.
REFERENCE   6  (bases 1 to 1539)
  AUTHORS   Yada,H., Murata,M., Shimoda,K., Yuasa,S., Kawaguchi,H., Ieda,M.,
            Adachi,T., Murata,M., Ogawa,S. and Fukuda,K.
  TITLE     Dominant negative suppression of Rad leads to QT prolongation and
            causes ventricular arrhythmias via modulation of L-type Ca2+
            channels in the heart
  JOURNAL   Circ Res 101 (1), 69-77 (2007)
   PUBMED   17525370
REFERENCE   7  (bases 1 to 1539)
  AUTHORS   Ilany,J., Bilan,P.J., Kapur,S., Caldwell,J.S., Patti,M.E.,
            Marette,A. and Kahn,C.R.
  TITLE     Overexpression of Rad in muscle worsens diet-induced insulin
            resistance and glucose intolerance and lowers plasma triglyceride
            level
  JOURNAL   Proc Natl Acad Sci U S A 103 (12), 4481-4486 (2006)
   PUBMED   16537411
  REMARK    GeneRIF: These results demonstrate a potential synergistic
            interaction between increased expression of Rad and high-fat diet
            in creation of insulin resistance and altered lipid metabolism
            present in type 2 diabetes.
REFERENCE   8  (bases 1 to 1539)
  AUTHORS   Hawke,T.J., Kanatous,S.B., Martin,C.M., Goetsch,S.C. and Garry,D.J.
  TITLE     Rad is temporally regulated within myogenic progenitor cells during
            skeletal muscle regeneration
  JOURNAL   Am J Physiol Cell Physiol 290 (2), C379-C387 (2006)
   PUBMED   16221735
REFERENCE   9  (bases 1 to 1539)
  AUTHORS   Finlin,B.S., Crump,S.M., Satin,J. and Andres,D.A.
  TITLE     Regulation of voltage-gated calcium channel activity by the Rem and
            Rad GTPases
  JOURNAL   Proc Natl Acad Sci U S A 100 (24), 14469-14474 (2003)
   PUBMED   14623965
  REMARK    GeneRIF: both Rem and Rad bind directly to Ca2+ channel
            beta-subunits (CaV beta) in vivo
REFERENCE   10 (bases 1 to 1539)
  AUTHORS   Shimizu-Nishikawa,K., Tsuji,S. and Yoshizato,K.
  TITLE     Identification and characterization of newt rad (ras associated
            with diabetes), a gene specifically expressed in regenerating limb
            muscle
  JOURNAL   Dev Dyn 220 (1), 74-86 (2001)
   PUBMED   11146509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087473.1, AK159550.1 and AI894172.1.
            
            On Mar 4, 2019 this sequence version replaced NM_019662.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057138.1, SRR7345562.1088878.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849389, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-379               AK087473.1         2-380
            380-1535            AK159550.1         195-1350
            1536-1539           AI894172.1         5-8                 c
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.04 cM"
     gene            1..1539
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /note="Ras-related associated with diabetes"
                     /db_xref="GeneID:56437"
                     /db_xref="MGI:MGI:1930943"
     exon            1..167
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            168..601
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..1158
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /EC_number="3.6.5.2"
                     /note="Ras-like GTP-binding protein rad"
                     /codon_start=1
                     /product="GTP-binding protein RAD"
                     /protein_id="NP_062636.2"
                     /db_xref="CCDS:CCDS22584.1"
                     /db_xref="GeneID:56437"
                     /db_xref="MGI:MGI:1930943"
                     /translation="MTLNGGSGASGSRGAGRERDRRRGSTPWGPAPPLHRRSMPVDER
                     DLQAALAPGSLATTAAGTRTQGQRLDWPEGSSDSLSSGGSGSEEGVYKVLLLGAPGVG
                     KSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLLVYDIWEQDGGCWLPGHCMAM
                     GDAYVIVYSITDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGR
                     ACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKK
                     AKRFLGRIVARNSRKMAFRAKSKSCHDLSVL"
     exon            602..675
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            676..880
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1539
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1518..1523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /note="hexamer: AATAAA"
     polyA_site      1539
                     /gene="Rrad"
                     /gene_synonym="Rad; REM3"
                     /note="major polyA site"
ORIGIN      
        1 agcgccagct gaggggctga gaaagcagca gaggagaaaa agactgcagt cgtgacagcg
       61 acaagggtct gagtctgaac aggggtctac gagtgggttc tatcagatcc cccagtggtc
      121 tgtgtcgcag cgctgatcaa gtcctgataa catctgtgtg agagttgagt cctacctgct
      181 cccgcgatag agctgccatc ccaattcttc gttccccaga cggcgtccaa cacgatgacc
      241 ctgaatggtg gcagcggagc aagcgggagc cgtggtgcgg gccgggagcg cgaccgccgt
      301 cgaggcagca ccccatgggg cccggcaccc ccactgcacc gcaggagcat gccggtggac
      361 gagcgagacc ttcaggcggc gctagccccg ggctccctgg caacgactgc cgctggaact
      421 cggacacagg gccagaggct ggactggccc gaaggctcct ccgactcgct cagttctgga
      481 ggcagtgggt cagaggaggg cgtttacaag gtgctgctgc tcggggcgcc tggtgtgggc
      541 aaaagcgctc tggcgcgcat ctttggtggt atagaagacg gacctgaagc agaagcagca
      601 gggcacacat atgaccgttc tatcactgtg gatggagaag aagcgtcact attggtctat
      661 gacatttggg aacaggatgg gggctgctgg ctgcctggcc actgcatggc catgggggat
      721 gcatacgtca tcgtgtactc catcacggac aagggcagct ttgagaaagc ctcagaactc
      781 cgggtccagc tgcggcgggc ccggcagaca gacgacgtgc ccatcattct cgtgggcaac
      841 aagagtgacc tggtgcgctc tcgtgaggtc tctgtggatg agggccgggc ctgcgcagtg
      901 gtcttcgact gcaagttcat cgagacatca gcagcactgc atcacaatgt ccaggcacta
      961 ttcgaggggg ttgtgcgcca gatacgcctg cgcagggaca gcaaagaaga taacgcccgg
     1021 cggcaagctg gcactcggcg acgggagagc cttggcaaga aggccaaacg ctttctgggc
     1081 cgcatcgtgg ctcgcaacag ccgcaagatg gccttccgcg ccaagtccaa gtcctgccat
     1141 gacctctctg ttctctaagg cccaatggcc cccttgctta atgggccagt aaatggctgg
     1201 agggactggc ccaggcaact gttctagatg tctcagagtt ggttcatcct ctgtgtttct
     1261 cagaactgtc aactatgcag cacacaccct tgtctcatgg tcatggacag aaagatagtg
     1321 ctgaccaggg aggcaactcc aaatggccag agaggcctag gcccatggag atgcattgtg
     1381 caggcccaca tgcataccaa gcagcctgaa cccagcttgt ggacaggact ctgcctttct
     1441 ctatgcgggt gtctgtgccc tggagggagg ctgttcaata tggttgctgt ttgtttacat
     1501 gcagattttt tttttgtaat aaagactatt tcctgacaa
//
