LOCUS       NM_001033204            1269 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus RNA pseudouridylate synthase domain containing 3
            (Rpusd3), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001033204 XM_132830 XM_924644
VERSION     NM_001033204.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153910.6.
            
            On or before Apr 11, 2009 this sequence version replaced
            XM_924644.2, XM_132830.8.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC116827.1, SRR5189685.56886.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AC153910.6         94359-94481         c
            124-263             AC153910.6         93951-94090         c
            264-308             AC153910.6         93172-93216         c
            309-408             AC153910.6         92988-93087         c
            409-516             AC153910.6         92445-92552         c
            517-601             AC153910.6         92282-92366         c
            602-725             AC153910.6         91930-92053         c
            726-865             AC153910.6         91448-91587         c
            866-1269            AC153910.6         90460-90863         c
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 52.76 cM"
     gene            1..1269
                     /gene="Rpusd3"
                     /note="RNA pseudouridylate synthase domain containing 3"
                     /db_xref="GeneID:101122"
                     /db_xref="MGI:MGI:2141440"
     exon            1..123
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1057
                     /gene="Rpusd3"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     pseudouridylate synthase domain-containing protein 3;
                     mitochondrial mRNA pseudouridine synthase Rpusd3"
                     /codon_start=1
                     /product="mitochondrial mRNA pseudouridine synthase Rpusd3
                     isoform 1"
                     /protein_id="NP_001028376.1"
                     /db_xref="CCDS:CCDS51872.1"
                     /db_xref="GeneID:101122"
                     /db_xref="MGI:MGI:2141440"
                     /translation="MGALRVLRYVSMIWRPELGSCARQRDAGFGTEARRPSQPHRSSK
                     HKDLVEDQPFPGLLRTENLGLEELAHVLRAAVVDQKGPLVTLNKPQGLPVTGRPGELT
                     LLSVLPQLSQALGLEHQELQVVRAPGKEASGLVLLSSCPQTASRLQKFFIHSRRAQRP
                     TATYCAVTDGIPEPSEGTVCMPLKMEQMNDVDLAVPVMSPSRKDIQEGVKRTLSRFHV
                     MATGRGCALVQLQPLTVFPNQLQVHMALQLCPILGDHTYAARVGTVLGQRFLWPAETT
                     KPQRQVLDEALLRHLRLSPSQVAQMPLHLHLHRLLLPGTGSRDPPSELLAPLPPYFSR
                     TLQCLRLSQQ"
     exon            124..263
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            264..308
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            309..408
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            409..516
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            517..601
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            602..725
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            726..865
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1269
                     /gene="Rpusd3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1223..1228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd3"
                     /note="hexamer: AATAAA"
     polyA_site      1269
                     /gene="Rpusd3"
                     /note="major polyA site"
ORIGIN      
        1 gtactgtgct tggtccccgg caatgggagc cctgagagtt ttgcgttatg tctctatgat
       61 ctggcggccg gagctggggt cttgcgcacg gcagagggat gccggcttcg gcaccgaggc
      121 ccggcgtccg tctcaacctc accgctccag caagcacaag gacctagtcg aggaccagcc
      181 cttcccgggg ctgctgagga cggagaacct cggcctggag gagctggctc atgtcctgag
      241 ggcagctgtg gtggatcaga aaggacctct ggtgacactg aacaagccac agggtctgcc
      301 tgtgacaggg agaccaggag agctgacgct gctatctgtg ctgccgcagc tgagccaggc
      361 cctggggctc gaacaccagg agctccaggt tgtgcgagca cctgggaagg aggcctccgg
      421 acttgtgctc ctctccagct gtccccagac agcaagccgc ctgcagaagt tctttatcca
      481 ctcaaggaga gcacagagac ccacagccac ctactgtgct gtcactgatg gcatccctga
      541 gccttcagag gggacagtct gcatgcctct gaagatggaa caaatgaatg acgttgatct
      601 tgcagtccca gtgatgtccc catctcgaaa ggacatccag gaaggtgtca aaaggaccct
      661 cagccgtttc catgtgatgg ccacaggccg tggctgtgcc ctggtccagc tgcagccact
      721 gacagtcttc cccaatcagc tgcaggtaca catggctcta cagctctgtc caattcttgg
      781 ggaccacact tatgctgctc gtgtgggcac cgttctgggc cagcgctttc tgtggccagc
      841 cgagaccacc aagccccaga gacaggtcct ggatgaagcc ctgctcagac acctccgcct
      901 aagtccttcc caagtggccc agatgccttt gcacctgcac cttcaccgcc tcctcctccc
      961 aggcaccgga tccagggacc cccctagtga acttctggca cccctgcccc cttacttctc
     1021 tcggaccctg cagtgccttc ggctctctca acagtagact gtgcctgacc ccagatggaa
     1081 aataggaaga ataaggcgag tggagttggt gctgagcaca gccaaggccc ctgggctcct
     1141 tggtctgaca aactaagatc aaatcccaga gaggccacgt gattgctgtg tggcactggg
     1201 caggtgactt ctctctggac ttaataaaag ttctacctcc tggtctggtt taccagaaat
     1261 gagaaagca
//
