LOCUS       NM_173450               5135 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus RNA pseudouridylate synthase domain containing 2
            (Rpusd2), mRNA.
ACCESSION   NM_173450
VERSION     NM_173450.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044767.1, BB124857.2 and AL772264.8.
            
            On Apr 28, 2009 this sequence version replaced NM_173450.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK044767.1, BC117827.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2133              AK044767.1         2-2134
            2134-2333           BB124857.2         550-749
            2334-5135           AL772264.8         169993-172794       c
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.84 cM"
     gene            1..5135
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="RNA pseudouridylate synthase domain containing 2"
                     /db_xref="GeneID:271842"
                     /db_xref="MGI:MGI:1918066"
     exon            1..679
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1696
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="RNA pseudouridylate synthase domain-containing
                     protein 2"
                     /codon_start=1
                     /product="pseudouridylate synthase RPUSD2"
                     /protein_id="NP_775626.1"
                     /db_xref="CCDS:CCDS16588.1"
                     /db_xref="GeneID:271842"
                     /db_xref="MGI:MGI:1918066"
                     /translation="MWRGVPGCLRDIVQWQVALWSHSFVRTWGSCGKAMTEALSAQAE
                     AAGGLKALVQPNGDAGSNTSGEPLLERLEPAAVGKQVPESGDQAQGGEGQLPSNGEQT
                     PAPVADSGKRKKRRGATGERVVPPPKKRRTGVSFSDEHFAETTYYFEGGLRKVRPYYF
                     DFQTYCKGRWVGRSLLHVFSTEFRSQPLSYYEAAVRAGRLHLNEEPVQDLSIVLKDND
                     FLRNTVHRHEPPVTAEPIHLLAENNDVVVIDKPSSIPVHPCGRFRHNTVIFILGKEHQ
                     LKELHPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAGEFPDKEVIC
                     KEPILVVSYKVGVCRVDPRGKPCETVFQRLSYNGRSSVVQCRPLTGRTHQIRVHLQFL
                     GHPILNDPIYNSTAWGPSRGQGGHIPKTDEELLRDLVAEHQAKESLSMLDLCESDLTP
                     GLIDSTAPSSELAKDSLEGLATAAQKIDGIAEAAPQHLDTPEKAAKADVTQETDPLCA
                     ECRVLRQDPLPQDLVMFLHALRYKGPDFEYVSPIPAWARDDWQED"
     exon            680..976
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /inference="alignment:Splign:2.1.0"
     exon            977..5135
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1896
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="major polyA site"
     regulatory      2088..2093
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="hexamer: AATACA"
     polyA_site      2107
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="major polyA site"
     regulatory      3553..3558
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="hexamer: ATTAAA"
     polyA_site      3576
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
     regulatory      3601..3606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="hexamer: AATAAA"
     polyA_site      3632
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
     regulatory      4099..4104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="hexamer: AATAAA"
     polyA_site      4120
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
     regulatory      5106..5111
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
                     /note="hexamer: ATTAAA"
     polyA_site      5135
                     /gene="Rpusd2"
                     /gene_synonym="4921503C21Rik; 9630001E10"
ORIGIN      
        1 agattgcgac cctgaagcgg ctagccgtgc tgtcatgtgg cggggtgtcc cgggctgcct
       61 acgggacatt gtgcagtggc aggtggcgct ctggtcccac agctttgtcc ggacttgggg
      121 tagctgtggg aaggcgatga ctgaggcact gtctgcgcaa gccgaggcag cgggcgggct
      181 gaaggctttg gttcagccaa atggagatgc tggtagcaac acgagcggag agccgctcct
      241 ggagcgcctg gagccggcgg cggtagggaa gcaggtcccg gagagcgggg accaggccca
      301 gggaggcgaa gggcagctcc cgagcaacgg agagcagacc ccagctccag tcgccgactc
      361 aggcaagagg aaaaagcgga ggggcgccac tggggaacgg gtcgtgccgc ctccgaagaa
      421 acggcgcacc ggggtgagct ttagcgatga gcacttcgca gagactacct actacttcga
      481 gggcggcctg cgcaaggtgc ggccctatta ctttgacttc cagacctact gtaaaggtcg
      541 ctgggtgggc cgcagcttgt tgcacgtctt cagcaccgag ttccgatccc agccactgtc
      601 ttactacgag gccgctgtcc gggcaggacg cctgcatctc aacgaggaac cagtccagga
      661 cctcagcatc gtgctcaagg acaatgattt cttgcggaac acagtgcaca gacatgagcc
      721 accagtcaca gcagagccta tccacctgct agctgagaac aacgatgtgg ttgttataga
      781 caagccgtcc tccattcctg tgcacccctg tggccgcttc cgacacaaca cggtcatctt
      841 catcctaggc aaggagcacc aactgaaaga gctacaccca ctgcatcggc tggaccgcct
      901 cacctcagga gtgctcatgt ttgcgaagac agctgcagtc tcggagaaga tccatgagca
      961 ggttcgggat cggcagctgg agaaggagta tgtatgccgg gttgcagggg agttccctga
     1021 caaggaggta atttgtaagg aacccatctt agtggtgtct tacaaggtag gagtgtgccg
     1081 tgtggatccc cggggcaagc cttgtgagac tgtgttccag aggctgagct acaatggccg
     1141 ctccagtgtg gtacagtgcc gaccacttac aggtcgcact caccagatcc gagtccacct
     1201 ccaattcctg ggccacccga ttctcaatga ccccatctac aattcaactg cctggggtcc
     1261 ttctcgaggc cagggtggcc acattccaaa gacagacgag gaactgctcc gagacctggt
     1321 tgcagaacat caggccaaag aaagcctgag tatgctagat ctctgtgaga gtgacctgac
     1381 gccagggctc atagactcga cagctccctc ttcagagttg gctaaagaca gcctggaagg
     1441 gttggctaca gcagcccaga agatagatgg aatagctgag gcagcccctc agcatctgga
     1501 cacaccagag aaggcagcta aagcagatgt gactcaagaa acagacccgc tctgtgccga
     1561 gtgccgagtg ttgcgacagg atcccttgcc ccaggatctc gtaatgttcc tgcatgccct
     1621 ccgctataaa gggccagact ttgagtacgt ctcacccatc ccagcctggg cacgggatga
     1681 ctggcaagaa gactgaggaa tgtgaccatt ggaaaaatgg cttcttggat tggagcaagg
     1741 atgggccttg agacagggac tgctcccatt ggttgatact caggtttgga gaagagcaag
     1801 gtttcacagt aaggaacctt gcttgtacct gctgctacct ccttccttct ggcttgttgg
     1861 ggctttcaga tctgtacata gatcattttt ttctaacacc ttgtatgtcc atttttactg
     1921 gtttcttacg atcccttccc tcttcccccc agtgcttagg atagaatcta gatccattag
     1981 aaggcaagtg ctctacaact gacctccaag ccccttagta tttctgagtt tctctagctc
     2041 aggcagatct tgaaccctga atcctcctac attgcctcca gagcgggaat acaggtatga
     2101 aacaccatgc cttgcttttt tttttttttt ttttttttaa ttgagataaa attcacatag
     2161 cataaaatta accgctttaa gataaacact gttggcattt actatattca caaactagag
     2221 caaccttatc tcaagatttc aaaacatatt tctcattcct acaggaaact gtacagcaca
     2281 ttcttccctt tttctctctc tcaactcctg gtaaacagtt tattctttat tccacaggca
     2341 tggactcaga caatctgtgt ctttttcttt tttcttttgt tctttgttgt tgtatgtttg
     2401 ttttctgaga catggttttg gtatagccct ggctgtcctg gatcttgctc tgtagaccaa
     2461 gctggtcttg aactcacaaa gatccacctg catctgcctt ctgagtgctg ggattcaagg
     2521 agtgtgccac gccccccaag ctacccaaca cttattcttt tctgtttgtt ctactgatca
     2581 tctctctcct agtgcatgtg atataatttc tcacagtgga atatactctt tttgttgtgt
     2641 tgtttattat ttattctgga cataaaatct taacaaaaga attaacagcc caattacaaa
     2701 atatccaaaa atgattctgg ttctctctct ctctctctct ctctctctct ctctctctct
     2761 ctttctctct ctctcacaca cacacacaca tacacacaca cacacacaca cacacacaca
     2821 cacggtctgg ttaagagcac tcattgctct ttgagtggct cacaaccacc tgacacccca
     2881 gcattagggg acccttcccc tcttctggcc tctgccaggg ctacatagtg agactcatgt
     2941 ctcaaaataa caaagacgga agcagtgcag ttaacaaaac taacatgttt tagaatggca
     3001 gatttcctgg agtcaggact gttaccacca gagcactgac tctgttatga tgccttgcct
     3061 tccacaggta agcactgtgc gtgatcacgg ctgtctttcc actccaaatg agctcttctc
     3121 tcatgtgaag cgcattctta ttttcaaaac cacattttat cagccattag ccacatcaaa
     3181 ataattggaa aatgtctgtc tattgctaaa gctgcctctg tgtgtatgtg gggcgggggg
     3241 ggggggtgga gtgggagagg ggagtgtact gagaccctgt gtcaaacaaa gaaaataaca
     3301 cacacagaca tttttgtaag ccttgaatct ggggtaactc ctctcctcct ccatctttcc
     3361 tgctctctct ctctgttttt cttgagaggg tcttgttatg tagccaggat agcctcagaa
     3421 tcactaagta tcccagctgg cctcacactc agtttctact ttcttaagct ttccaattgc
     3481 ttgagttata gacagccatg tacaagcttg ccttgaagtt tttgtttttg tttttaaatc
     3541 ccaaatggta gaattaaaca catgcactgc cactcaggat tttgaagatt tcttgtgtat
     3601 aataaaacat agactataag caaacaaaca tacaaccatc cattgcaatt ctttcagcgc
     3661 tacgtcttaa aagagttgtt gatgtataac catccaggac tgtggcgcat caccagctga
     3721 caattcttcc ctgataagtt cataatcaac agagggaatg agtcttcctg aagctgaatc
     3781 tagaggatta agacccattt tttagcactt aagcagtaaa tgctgttagt cttaattagg
     3841 tatttttgta tcaatctttt ctcatggtct tccagggact cggggttgga atatcctgta
     3901 atagttcagg atagtgtgaa gtaaccttgc tgttgatttg agtgctttga gcaggattta
     3961 aagggtaaaa acatgaaacc tccttctcac ccaaagctgg aagtagccac tgatccagta
     4021 gatggcgcct ctcactcttt tatcagtctc ttccctttct gttactaatt caagtaattt
     4081 ccccctcccg ttctttaaaa taaagtggta ttgttgatta ttttcactcc cttagaaaat
     4141 gggagccaag tactggtagt tatgaatcag gaaatcatca ggagagaaaa ccctggtatt
     4201 ctcataacaa gcctcacggt aatttcatgt gagagtaaga actaaacacc ctgacttaca
     4261 aaggcatggc agcaagaggt tcaggagtga gcctcttgaa actggatacc tgcatcacac
     4321 ctgccaggct cagaacatat gggagaagac tgggaaagaa gggaagagcc agaggataag
     4381 gaggggtgcc ttaaaaagtc tgtggggagt ggctgggttg ttgcacccgt gcagcagtag
     4441 ttacctctgc aagccaggcg caattctgag tccactgtca tttaaacatg ggtgagggag
     4501 gggctactgg cagttaatgg ttgctgggag attggatgcc attttcttca gtgatctaac
     4561 taatgaaaag ttgcacagct tctgctgtgc aaactgagac tgaatcagag atggatatcc
     4621 attggataaa gttttaacca gtttttcttc aacatgaaac atagaacgac agtcattcag
     4681 acaaaacaaa aacacacaaa ctgacacatg gtcagactgg tgcaccaagg acagacaatg
     4741 cagagaagga aggaacttga tgtaaccaga actaaggatg cctactgtag gggccagaga
     4801 ggaagcaaga agtctcccga tttccttctg tccctgggag agctctgaaa tccattttcc
     4861 ttcacttgat ctctctcatg tcatttgtat atctagcatc tcccttttgt tgtttttgtt
     4921 ttttattctt tttattttat cttatttatt ttattttaag ccctactcct ttcatcggat
     4981 acagttcttg actacagaca aatgtttgtt tagaattttc cagccccatt ttccactgtc
     5041 aacctctagc tgatatcagc attgggctga cgctgactca gtttaacctg tatccttttt
     5101 gcaccattaa aatcaacagc cttcaaaaga tgaaa
//
