LOCUS       NM_001114334            5535 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus ribosomal protein S6 kinase, polypeptide 1 (Rps6kb1),
            transcript variant 1, mRNA.
ACCESSION   NM_001114334
VERSION     NM_001114334.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Kawase,K., Nakamura,Y., Wolbeck,L., Takemura,S., Zaitsu,K.,
            Ando,T., Jinnou,H., Sawada,M., Nakajima,C., Rydbirk,R., Gokenya,S.,
            Ito,A., Fujiyama,H., Saito,A., Iguchi,A., Kratimenos,P.,
            Ishibashi,N., Gallo,V., Iwata,O., Saitoh,S., Khodosevich,K. and
            Sawamoto,K.
  TITLE     Significance of birth in the maintenance of quiescent neural stem
            cells
  JOURNAL   Sci Adv 11 (4), eadn6377 (2025)
   PUBMED   39841848
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Rosenlehner,T., Pennavaria,S., Akcabozan,B., Jahani,S.,
            O'Neill,T.J., Krappmann,D., Straub,T., Kranich,J. and Obst,R.
  TITLE     Reciprocal regulation of mTORC1 signaling and ribosomal
            biosynthesis determines cell cycle progression in activated T cells
  JOURNAL   Sci Signal 17 (859), eadi8753 (2024)
   PUBMED   39436996
  REMARK    Erratum:[Sci Signal. 2024 Dec 17;17(867):eadu7437. doi:
            10.1126/scisignal.adu7437. PMID: 39689184]
REFERENCE   3  (bases 1 to 5535)
  AUTHORS   Dong,M.Z., Ouyang,Y.C., Gao,S.C., Gu,L.J., Guo,J.N., Sun,S.M.,
            Wang,Z.B. and Sun,Q.Y.
  TITLE     Protein phosphatase 4 maintains the survival of primordial
            follicles by regulating autophagy in oocytes
  JOURNAL   Cell Death Dis 15 (9), 658 (2024)
   PUBMED   39245708
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5535)
  AUTHORS   Kim,D.E., Roh,H.S., Kim,G.H., Bhang,D.H., Um,S.H., Singh,R. and
            Baek,K.H.
  TITLE     S6K1 deficiency in tumor stroma impairs lung metastasis of melanoma
            in mice
  JOURNAL   Biochem Biophys Res Commun 696, 149469 (2024)
   PUBMED   38194806
  REMARK    GeneRIF: S6K1 deficiency in tumor stroma impairs lung metastasis of
            melanoma in mice.
REFERENCE   5  (bases 1 to 5535)
  AUTHORS   David,J., Mousset,M., Trombetti,K., Sayasouk,B., Neilsen,C.,
            Suorsa,P., Ruben,M., Ruben,E., Thiessen,J., Pychewicz,T., Chu,P.
            and Huynh,T.N.
  TITLE     Chronic mild stress leads to anxiety-like behavior and decreased
            p70 S6K1 activity in the hippocampus of male mice
  JOURNAL   Physiol Behav 273, 114377 (2024)
   PUBMED   37863347
  REMARK    GeneRIF: Chronic mild stress leads to anxiety-like behavior and
            decreased p70 S6K1 activity in the hippocampus of male mice.
REFERENCE   6  (bases 1 to 5535)
  AUTHORS   Pende,M., Kozma,S.C., Jaquet,M., Oorschot,V., Burcelin,R., Le
            Marchand-Brustel,Y., Klumperman,J., Thorens,B. and Thomas,G.
  TITLE     Hypoinsulinaemia, glucose intolerance and diminished beta-cell size
            in S6K1-deficient mice
  JOURNAL   Nature 408 (6815), 994-997 (2000)
   PUBMED   11140689
REFERENCE   7  (bases 1 to 5535)
  AUTHORS   Matsuzaki,K., Katayama,K., Takahashi,Y., Nakamura,I., Udagawa,N.,
            Tsurukai,T., Nishinakamura,R., Toyama,Y., Yabe,Y., Hori,M.,
            Takahashi,N. and Suda,T.
  TITLE     Human osteoclast-like cells are formed from peripheral blood
            mononuclear cells in a coculture with SaOS-2 cells transfected with
            the parathyroid hormone (PTH)/PTH-related protein receptor gene
  JOURNAL   Endocrinology 140 (2), 925-932 (1999)
   PUBMED   9927325
REFERENCE   8  (bases 1 to 5535)
  AUTHORS   Shima,H., Pende,M., Chen,Y., Fumagalli,S., Thomas,G. and Kozma,S.C.
  TITLE     Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse
            phenotype and a new functional S6 kinase
  JOURNAL   EMBO J 17 (22), 6649-6659 (1998)
   PUBMED   9822608
REFERENCE   9  (bases 1 to 5535)
  AUTHORS   Kawasome,H., Papst,P., Webb,S., Keller,G.M., Johnson,G.L.,
            Gelfand,E.W. and Terada,N.
  TITLE     Targeted disruption of p70(s6k) defines its role in protein
            synthesis and rapamycin sensitivity
  JOURNAL   Proc Natl Acad Sci U S A 95 (9), 5033-5038 (1998)
   PUBMED   9560223
REFERENCE   10 (bases 1 to 5535)
  AUTHORS   Cheatham,L., Monfar,M., Chou,M.M. and Blenis,J.
  TITLE     Structural and functional analysis of pp70S6k
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11696-11700 (1995)
   PUBMED   8524831
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL604063.4, AK032724.1 and AK135716.1.
            
            On May 10, 2018 this sequence version replaced NM_001114334.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.12001.1, AK135716.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AL604063.4         64329-64367         c
            40-279              AK032724.1         2-241
            280-1815            AK135716.1         248-1783
            1816-5535           AL604063.4         18429-22148         c
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.82 cM"
     gene            1..5535
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="ribosomal protein S6 kinase, polypeptide 1"
                     /db_xref="GeneID:72508"
                     /db_xref="MGI:MGI:1270849"
     exon            1..242
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1679
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ribosomal protein S6 kinase beta-1; p70 S6 kinase alpha;
                     p70 ribosomal S6 kinase alpha; ribosomal protein S6 kinase
                     I; 70 kDa ribosomal protein S6 kinase 1; ribosomal protein
                     S6 kinase, 70kD, polypeptide 1"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase beta-1 isoform 1"
                     /protein_id="NP_001107806.1"
                     /db_xref="CCDS:CCDS48875.1"
                     /db_xref="GeneID:72508"
                     /db_xref="MGI:MGI:1270849"
                     /translation="MRRRRRRDGFYLAPDFRHREAEDMAGVFDIDLDQPEDAGSEDEL
                     EEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGY
                     GKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIY
                     AFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRD
                     LKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDW
                     WSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAAS
                     RLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPV
                     DSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVK
                     FSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTVSGEASAPLPIRQPNSGPYKK
                     QAFPMISKRPEHLRMNL"
     exon            243..292
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            293..413
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..482
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            483..630
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            631..688
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..789
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            790..880
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..971
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1079
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1142
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1220
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1328
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1441
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..5535
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2346..2351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="hexamer: AATAAA"
     polyA_site      2371
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="major polyA site"
     regulatory      3048..3053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="hexamer: AATAAA"
     polyA_site      3076
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
     regulatory      5367..5372
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="hexamer: ATTAAA"
     polyA_site      5395
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
     regulatory      5512..5517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
                     /note="hexamer: AATAAA"
     polyA_site      5533
                     /gene="Rps6kb1"
                     /gene_synonym="2610318I15Rik; p70 S6K-alpha; p70 S6KA;
                     p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1;
                     S6K1"
ORIGIN      
        1 tgaacttgag gagccagtct ggggcctagg cgcagacgca ttgagcttaa gcagccggtg
       61 atggcggcag cagccgtgga gtctgcggcg ggtccgggcc catgaggcga cgacggaggc
      121 gggacggctt ttacctagcg cctgacttcc gacacaggga agctgaggac atggcaggag
      181 tgtttgacat agacctggac cagccagaag atgcaggctc tgaggatgag ctggaggagg
      241 ggggtcagtt aaatgaaagc atggaccatg ggggagttgg accatatgaa cttggcatgg
      301 aacattgtga gaaatttgaa atctcagaaa ctagtgtgaa cagagggcca gaaaaaatca
      361 gaccagaatg ttttgagcta cttcgggtac ttggtaaagg gggctatgga aaggtttttc
      421 aagtacgaaa agtaacagga gcaaatactg ggaagatatt tgccatgaag gtgcttaaaa
      481 aggcaatgat agtgaggaat gctaaggaca cggcccacac gaaagcagag cggaacattc
      541 tggaggaagt gaaacaccct ttcattgtgg acctgattta tgcctttcag accggaggaa
      601 agctctacct catcctcgag tatctcagtg gaggagaact atttatgcag ttagaaagag
      661 agggaatatt catggaagac acagcgtgct tttacttggc tgaaatctcc atggctttgg
      721 ggcatttaca tcaaaaaggg atcatctaca gagacctgaa gccggagaac atcatgctta
      781 atcaccaagg tcacgtgaaa ctaacagact ttggactatg caaagaatct attcatgatg
      841 gaacagtcac gcacacattt tgtggaacaa tagaatacat ggcccctgaa atcttaatga
      901 gaagcggcca caaccgtgct gtggattggt ggagtttggg agcattaatg tatgacatgc
      961 tgactggagc acctccattc actggggaga atagaaagaa aacaattgac aaaatcctca
     1021 aatgtaaact taatttgcct ccctacctca cacaagaagc tcgagatctg cttaaaaagc
     1081 tgctgaaaag aaatgctgct tctcgtcttg gagctggccc tggggatgct ggagaagtcc
     1141 aagctcatcc ttttttcaga cacattaact gggaagaact tctggctcgg aaggtggaac
     1201 ctccctttaa gcctctgttg caatctgaag aggatgtgag tcagtttgat tcaaagttta
     1261 cacgtcagac acctgttgac agccctgatg actccactct cagtgaaagt gccaaccagg
     1321 tctttctggg ttttacatat gtggctccat ctgtacttga aagtgtgaaa gaaaagtttt
     1381 catttgaacc aaaaatccga tctcctagaa gatttattgg tagtccacga acacctgtca
     1441 gcccagtcaa attctctcct ggggatttct ggggacgagg tgcttcagcc agcacggcaa
     1501 atcctcagac ccctgtggaa tacccaatgg aaacaagtgg gatagagcag atggatgtga
     1561 cagtgagcgg ggaagcatca gcgccacttc caatccgaca acccaactcc gggccgtaca
     1621 aaaaacaagc ttttcctatg atctccaaac ggccagagca cctgcggatg aatctatgat
     1681 gaaacaatgc ttttattaat gcaaaatcaa aaaggaaaca aatccgggaa ggggatgtgt
     1741 gagcatcctg caatataaaa acaagaataa aatggcagtc tcaaagagtc agtgtcatta
     1801 cctggaatgc tttcgatgga ggaaaaaata aacatggatt ttaaaaaatc aatcaaaaat
     1861 ggtgcaaaaa aaaaaataaa cccacaaaaa actcaagcaa aatagtattg tggaatccac
     1921 agacacatca gctgactggt tcctatccta gcaacatctc ggcgttcgca agggttctca
     1981 tgctgatggc tgcaaactga cagtattaag ggtaggatgt tgcttcggaa tcaccgttga
     2041 aatctgatga tgtcaaataa gggttatcct aataggcaaa gtttgagatt gcctgtaata
     2101 cttgcaacta aggacaaatt agcatgcaag ctttgtcaaa ctttttccag caacacagaa
     2161 tcaaagacaa aagaaacttt atcgattgat gttttacgtg caaacaacct gaatcttttt
     2221 tttatataaa tatatatttt tcaaatagat ttttgattca gctcattatg aaaaacatcc
     2281 caaactttaa aatgcgaagt tattggttgg tgtgaagaaa gccagcttct gtcttctctt
     2341 gatgaaataa aatgcaaatg atcattgtta accacagctg tggcttgtct gagggcctgg
     2401 ggtggacctg gggtgttttt ttaatctttt tgttttagta acctagctgc aatacttgtc
     2461 tgtaatacta ggggggaaaa gtctgtttaa tcatttttac ttgcagtact gctgtgtgtt
     2521 tggcgtaact gcaagccttg ggacaggcag aagttgtatg atctacattg catccttgtc
     2581 ctgggcctgc actacactgg aaacagtatc accacctgtt cttataccag tatttgagtc
     2641 aagccttggt cgaggaggga cagaagagaa tcaggctaaa gtgcataaag agggcagtaa
     2701 gcgggggaga tagacctgca ggggagaggg tgttgccacg ggcgtctctc tcgactcttt
     2761 acagcacatt ggtgtggttc taggtttact ttgtactgtt atgctgttta ccttccttaa
     2821 caattttctt ttttgagaat ctaaaaaaga aaaaaatggt gtttttttcc cctcctgcac
     2881 tggggctaca tttttcactt ataaaaatat ttgatggcct tttgatgaat gtcttccata
     2941 acgaataaga aaacctagtg gcttaattta ggaaacatgt taacaagaca ctgtttttga
     3001 aattgtaaca aagtcaacat aagtgattta caggtacaaa gaataaaaat aaaggtaact
     3061 ttacctttct taaatacttc ctgccttaag gagcatttcc atgactagct ggtgaaaggg
     3121 tttaatatct gcagagcttt ataaaatata ctgcagtgca tactggtcta ggtagatggt
     3181 cacacagtga gtcctgtcac gccactctgt tggtcttttt tgtcttggta atagttgcag
     3241 cccttgggtt tctttatgca gatgctcttg ctgtgttcct caaaggatcc aagccataaa
     3301 attctttatg catgtcacag tcaggattga cccagacctt gcttattcaa attgtaattg
     3361 aagtgagatg ggaagaaatg ttaacagtga gcaaaaatag aaccttgaga ggctggagag
     3421 atgactcagc agttaagaac tctggctact tttccagagg tcctgagttc aatcccagca
     3481 cccacatgat agcttacatc tataaactgt agtctcgttg gatcaaatgt tctcttacat
     3541 gcacatgtgc agacaaaaca tccacataca taaaaatctt aaaaaaaaaa aacaaaaaaa
     3601 aaaaaccaga gccatggcag atgactggaa atactgccct tcaattgtgg cagcaaacgt
     3661 ccatctggac agagaatttc tttttttatc atttaaatgg tcattcctga ttctcttgac
     3721 tgtagcagca ttttgagaac ttcataattg tagcagtaaa ttaggagctg cagtaatgat
     3781 taagaatgtt cttcattctt tgagagtttg tggtatctta aaaaatagaa cccctcaaaa
     3841 aacctatggc aatgcattat aggaatcatt tcagactgca aatggcttgt gctactctga
     3901 tatctgtttc taaatgtgtt tactaactat agcgttgact gcctggccaa attccaataa
     3961 aacttttaca ctaaatgatt cctccttagc cctagttgct agtaatatgt gcactgtgac
     4021 caccccataa actgttaata aactgttcat agaattactg ccagcaatag tggcaaatat
     4081 cgcaacgttt ttgtgtagaa cgcattaatt gtacaccttc tgtcgacttc catctacaca
     4141 aatatatagc ttttctatca caacattaag tgaaattgat gctgtggcaa gtttattgag
     4201 aaactttcat aaatggatat ccctactatg actgtgaaaa catgtcaggt gtcacatgag
     4261 tgtcacagac agaaagcaca tgcctatgca atatggccta ctctgtattt atttgtaaaa
     4321 actgaagcat aatttaaagt gtatatcaat actactctga gtttctaaga gaggtgttca
     4381 tgcttgtacc aggtaagtga ataaaaaaaa agattaagtg ctttttcttt cattacttga
     4441 ttattttctt taaaatcagc tattacagga tattttttta ttttctacat tctgtttttt
     4501 aattaatatg tactcactga actgaaattt actaaatttg ttttataagg tttgtagtgt
     4561 tacagaataa ctaaactggg atttataaac cagctgtgat taacaatgta ttaattattt
     4621 gaacttgaac cagaccatta ggaaaattat gttatttgtt ccccttgtat gctcttaact
     4681 taaagaattt tcccacatac cgtttttgag atagtttgag atttgaggat aggatgcatg
     4741 tgagagactc cataaattca acattctgta ctatagctga caactataaa caaaatgtat
     4801 cttagcatta atatcttgag ccttgaacat catatttaat acaggaccat tttagaaata
     4861 ttcagttagt tgtatatttc ctaggttaca agggctagat ctaagattat tctcatgaga
     4921 aatgttgaat ttatgagaaa tggattttga ggctttgaaa atgaaaatgg ctagtatctc
     4981 aaagatgtca gtatccatac atctatggtt tcatcccagg agcagacatt agctagctgg
     5041 acaaacagtt gtgacaatat ttgtcacata ggggcctatg gtttgttgct caacaatata
     5101 gtacttttct catgtgactc aacactatag cacaagtatt atttccctgg attctctgaa
     5161 ggagcatcta aaagacgggt ttctgacagt gtttgctctt taaactatgt tttctcctac
     5221 cccaagttgg ctttgcatct attaaataag ttcttcagct gccttactag gagttctacg
     5281 agggcaacat cttttctgca tttcaccttg tatttagttt actgtgttaa gatatttgat
     5341 ttcagattga atgaatgtaa atagaaatta aatgcaaatt tgaatgaaca taaaatagaa
     5401 gtgatttttt attacttgag agaaaaaaat tttagtatat ttactatagc atacataaca
     5461 gatgtacaat tttttcatta acgtgttaga tatgttcatg ttaccatatt taataaacta
     5521 cttttgtata aaaga
//
