LOCUS       NM_001330702            5657 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus ribosomal protein S6 kinase, polypeptide 5 (Rps6ka5),
            transcript variant 2, mRNA.
ACCESSION   NM_001330702 XM_006516274
VERSION     NM_001330702.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   More,L., Privitera,L., Lopes,M., Arthur,J.S.C., Lauterborn,J.C.,
            Correa,S.A.L. and Frenguelli,B.G.
  TITLE     MSK1 is required for the experience- and ampakine-dependent
            enhancement of spatial reference memory and reversal learning and
            for the induction of Arc and BDNF
  JOURNAL   Neuropharmacology 261, 110110 (2024)
   PUBMED   39128584
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   More,L., Privitera,L., Cooper,D.D., Tsogka,M., Arthur,J.S.C. and
            Frenguelli,B.G.
  TITLE     MSK1 is required for the beneficial synaptic and cognitive effects
            of enriched experience across the lifespan
  JOURNAL   Aging (Albany NY) 15 (13), 6031-6072 (2023)
   PUBMED   37432063
  REMARK    GeneRIF: MSK1 is required for the beneficial synaptic and cognitive
            effects of enriched experience across the lifespan.
REFERENCE   3  (bases 1 to 5657)
  AUTHORS   Ji,C.H., Gu,J.H., Liu,Y., Tang,W.Q., Guan,W., Huang,J., Chen,Y.M.,
            Xu,D.W. and Jiang,B.
  TITLE     Hippocampal MSK1 regulates the behavioral and biological responses
            of mice to chronic social defeat stress: Involving of the BDNF-CREB
            signaling and neurogenesis
  JOURNAL   Biochem Pharmacol 195, 114836 (2022)
   PUBMED   34774532
  REMARK    GeneRIF: Hippocampal MSK1 regulates the behavioral and biological
            responses of mice to chronic social defeat stress: Involving of the
            BDNF-CREB signaling and neurogenesis.
REFERENCE   4  (bases 1 to 5657)
  AUTHORS   Nemska,S., Daubeuf,F., Obrecht,A., Israel-Biet,D., Stern,M.,
            Kessler,R., Roux,A., Tavakoli,R., Villa,P., Tissot,A., Danger,R.,
            Reber,L., Durand,E., Foureau,A., Brouard,S., Magnan,A. and
            Frossard,N.
  CONSRTM   COLT consortium
  TITLE     Overexpression of the MSK1 Kinase in Patients With Chronic Lung
            Allograft Dysfunction and Its Confirmed Role in a Murine Model
  JOURNAL   Transplantation 105 (6), 1212-1224 (2021)
   PUBMED   33560725
  REMARK    GeneRIF: Overexpression of the MSK1 Kinase in Patients With Chronic
            Lung Allograft Dysfunction and Its Confirmed Role in a Murine
            Model.
REFERENCE   5  (bases 1 to 5657)
  AUTHORS   Lee,W.S., Lavery,L., Rousseaux,M.W.C., Rutledge,E.B., Jang,Y.,
            Wan,Y.W., Wu,S.R., Kim,W., Al-Ramahi,I., Rath,S., Adamski,C.J.,
            Bondar,V.V., Tewari,A., Soleimani,S., Mota,S., Yalamanchili,H.K.,
            Orr,H.T., Liu,Z., Botas,J. and Zoghbi,H.Y.
  TITLE     Dual targeting of brain region-specific kinases potentiates
            neurological rescue in Spinocerebellar ataxia type 1
  JOURNAL   EMBO J 40 (7), e106106 (2021)
   PUBMED   33709453
  REMARK    GeneRIF: Dual targeting of brain region-specific kinases
            potentiates neurological rescue in Spinocerebellar ataxia type 1.
REFERENCE   6  (bases 1 to 5657)
  AUTHORS   Reyskens,K.M. and Arthur,J.S.
  TITLE     Emerging Roles of the Mitogen and Stress Activated Kinases MSK1 and
            MSK2
  JOURNAL   Front Cell Dev Biol 4, 56 (2016)
   PUBMED   27376065
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5657)
  AUTHORS   Wiggin,G.R., Soloaga,A., Foster,J.M., Murray-Tait,V., Cohen,P. and
            Arthur,J.S.
  TITLE     MSK1 and MSK2 are required for the mitogen- and stress-induced
            phosphorylation of CREB and ATF1 in fibroblasts
  JOURNAL   Mol Cell Biol 22 (8), 2871-2881 (2002)
   PUBMED   11909979
REFERENCE   8  (bases 1 to 5657)
  AUTHORS   Zhong,S., Jansen,C., She,Q.B., Goto,H., Inagaki,M., Bode,A.M.,
            Ma,W.Y. and Dong,Z.
  TITLE     Ultraviolet B-induced phosphorylation of histone H3 at serine 28 is
            mediated by MSK1
  JOURNAL   J Biol Chem 276 (35), 33213-33219 (2001)
   PUBMED   11441012
REFERENCE   9  (bases 1 to 5657)
  AUTHORS   Arthur,J.S. and Cohen,P.
  TITLE     MSK1 is required for CREB phosphorylation in response to mitogens
            in mouse embryonic stem cells
  JOURNAL   FEBS Lett 482 (1-2), 44-48 (2000)
   PUBMED   11018520
REFERENCE   10 (bases 1 to 5657)
  AUTHORS   Deak,M., Clifton,A.D., Lucocq,L.M. and Alessi,D.R.
  TITLE     Mitogen- and stress-activated protein kinase-1 (MSK1) is directly
            activated by MAPK and SAPK2/p38, and may mediate activation of CREB
  JOURNAL   EMBO J 17 (15), 4426-4441 (1998)
   PUBMED   9687510
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159818.2, CX207283.1, AK032316.1, AK160758.1, AC123705.12 and
            BQ174267.1.
            
            On Sep 8, 2016 this sequence version replaced XM_006516274.3.
            
            Transcript Variant: This variant (2) lacks an exon in the coding
            region, compared to variant 1. The encoded isoform (2) is shorter,
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032316.1, AY341881.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AC159818.2         100096-100162
            68-659              CX207283.1         1-592
            660-1259            AK032316.1         661-1260
            1260-3931           AK160758.1         127-2798
            3932-4301           AK032316.1         3933-4302
            4302-4944           AC123705.12        117927-118569       c
            4945-5657           BQ174267.1         1-713               c
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 50.81 cM"
     gene            1..5657
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /note="ribosomal protein S6 kinase, polypeptide 5"
                     /db_xref="GeneID:73086"
                     /db_xref="MGI:MGI:1920336"
     exon            1..340
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..2637
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ribosomal protein S6 kinase alpha-5; RSK-like protein
                     kinase; 90 kDa ribosomal protein S6 kinase 5; nuclear
                     mitogen- and stress-activated protein kinase 1"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase alpha-5 isoform 2"
                     /protein_id="NP_001317631.1"
                     /db_xref="CCDS:CCDS88388.1"
                     /db_xref="GeneID:73086"
                     /db_xref="MGI:MGI:1920336"
                     /translation="MEGEGGGSGGAGTSGDSGDGGEQLLTVKHELRTANLTGHAEKVG
                     IENFELLKVLGTGAYGKVFLVRKISGHDAGKLYAMKVLKKATIVQKAKTTEHTRTERQ
                     VLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEI
                     VLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIEY
                     MAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPP
                     YPQEMSTVAKDLLQRLLMKDPKKRLGCGPRDAEEIKEHLFFEKIKWDDLAAKKVPAPF
                     KPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSERLFQGYSFVAPSILFKRNAAVIDP
                     LQFHMGVDRPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKTNQA
                     FAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFER
                     IKRKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKVIDFG
                     FARLKPPDNQPLKTPCFTLHYAAPELLTHNGYDESCDLWSLGVILYTMLSGQVPFQSH
                     DRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLRY
                     NEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPLA
                     KRRKMKRTSTSTETRSSSSESSRSSSSQSHGKTTPTKTLQPSNPTEGSNPDTLFQFSD
                     "
     exon            341..412
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            413..631
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            632..747
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            748..855
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            856..939
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1043
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1194
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1356
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1482
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1616
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1710
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1881
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..2073
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2233
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2397
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..5642
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4283..4288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /note="hexamer: AATAAA"
     polyA_site      4303
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
     regulatory      5623..5628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /note="hexamer: AAGAAA"
     polyA_site      5642
                     /gene="Rps6ka5"
                     /gene_synonym="3110005L17Rik; 6330404E13Rik; MSK1; MSPK1;
                     RLPK; RLSK; S6K-alpha-5"
                     /note="major polyA site"
ORIGIN      
        1 gggaggagcg gcctggagga ggaggtggag gcgcagggcg cggggttgtg ccgtactagc
       61 ggtgcccgct gaggggagga ggcggaggag ctgcacggcc agagcgggaa agagactcgc
      121 ctcagtgtcg gagttgtggc gctgcggcag ctcggctcct ggagctgtgg agagacgggg
      181 cgtctgctgt cccatcaggt tcccctaaga gcgacccgcg cgatcggaaa gagggtgaag
      241 atggaggggg agggcggcgg cagcggcggc gcgggcacca gcggggacag cggcgacggc
      301 ggcgagcagc tccttactgt caagcacgag ctgcggaccg ccaacttaac aggacacgca
      361 gagaaggtgg ggattgaaaa ctttgagctg ctgaaggtcc taggaactgg agcttatggg
      421 aaagtatttc tagttcgtaa aataagcggc catgatgctg gaaagctgta tgctatgaag
      481 gttttaaaaa aggcaacaat agttcagaag gctaaaacta cagagcatac aaggacagaa
      541 cgacaggttc tggagcacat tagacagtcg ccatttttgg tgacattgca ctatgctttc
      601 cagacagaga ccaagcttca tctcatctta gattatataa atggtggaga actttttact
      661 catctttctc aaagagagcg tttcacagag cacgaagtgc agatctatgt tggagagatt
      721 gtgcttgccc tggaacacct gcacaagttg gggattatat accgtgacat taagcttgaa
      781 aatattctcc tggattctaa cggccatgtg gtgctgacag atttcggtct gagtaaggag
      841 tttgtggccg atgaaactga aagagcatat tctttttgtg gaactattga atacatggct
      901 ccagatattg tcagaggggg tgattcggga cacgacaagg cagttgactg gtggagctta
      961 ggtgttctaa tgtatgagct actaactgga gcatctcctt tcactgtcga tggagaaaaa
     1021 aattcccagg ctgagatatc taggagaatt ttaaaaagtg agcctccgta tccccaggaa
     1081 atgagcacag tagctaaaga cctacttcag cgtctcttga tgaaagatcc caagaagcga
     1141 ttgggatgtg gcccacggga tgcagaagaa atcaaagaac atctcttctt tgagaagata
     1201 aagtgggatg acttagctgc caaaaaagtg cctgcgccat tcaagccggt gatccgggat
     1261 gagttagatg tgagtaactt cgctgaagag ttcacagaga tggaccccac ctactctccc
     1321 gctgccttgc cacagagctc cgagcgactg tttcagggct attccttcgt tgctccatct
     1381 attctcttca agcgtaacgc agctgtcata gatcctcttc agttccatat gggagttgac
     1441 cgtcctggag tgacaaatgt tgccaggagt gcaatgatga aggactcacc cttctatcaa
     1501 cactatgacc tagatctgaa ggacaaacct ttgggagaag gaagtttttc aatttgtcgc
     1561 aagtgtgtac acaaaaaaac taaccaagca tttgctgtca aaataatcag caaaaggatg
     1621 gaagccaata ctcagaaaga aataacagct ctgaaactct gcgaaggaca ccccaatatt
     1681 gtgaaactac atgaagtttt tcacgatcag cttcatacgt ttcttgtgat ggaacttctg
     1741 aatggcggag agctgttcga gcgcatcaag agaaagaagc acttcagcga gactgaggcc
     1801 agctacatca tgcggaagct tgtgtcagct gtgagccaca tgcacgatgt aggagtggtg
     1861 cacagagatc tgaagcctga gaatctgttg ttcactgatg aaaacgacaa tttggaaatt
     1921 aaagtgattg actttgggtt tgcacgcctc aagccacctg acaatcagcc cctcaagacc
     1981 ccgtgcttca ccctccacta cgcagcaccg gagctcttga cgcacaatgg ctacgatgag
     2041 tcctgtgacc tgtggagctt gggtgtcatc ctgtatacaa tgctgtcagg gcaggtgcca
     2101 ttccagtctc atgacagaag tttaacatgc accagtgcag tggaaatcat gaagaaaatt
     2161 aaaaagggag acttctcctt tgaaggagaa gcgtggaaga atgtatccca agaggctaaa
     2221 gatttgattc aagggcttct tacagttgat ccaaataaaa ggctgaaaat gtctggtttg
     2281 agatacaatg aatggcttca ggatggcagt cagctgtcct ccaatcctct gatgactcca
     2341 gacattctgg gatcgtcagg agcagctgtg cacacctgtg tgaaagcaac ctttcatgcc
     2401 tttaacaagt ataagagaga aggattttgc ctgcagaatg tggataaggc ccctttggct
     2461 aagaggagga aaatgaagag gaccagcacc agcacagaga ctcgcagcag ctccagcgag
     2521 agctcccgct cctcctcctc ccagtcccac ggcaagacaa cgcctaccaa gacgctgcag
     2581 cccagcaatc ccaccgaagg cagtaaccca gacaccctct tccagttctc agactgaccc
     2641 ttgcacctca gctccctcag catgtgcctg aggtatgttc tgcgctttaa aagatgtgtc
     2701 tcgctgttct cattggaatc tgcctcgtga cgaatatttt ccggggaagc ctatttggtt
     2761 atccttgtcc aaaagcactg gacagacagc gttactgtga atagaggaca cgtgagagat
     2821 actttttagc agactaacat gatgccagct caagattctt cttgaaacag caaaggtttc
     2881 tgtaacttta gtgactggtt agctaagtca cagattttca cccaggtgtt tccaccaaga
     2941 tgtctgccaa gaagacttga agacttctgc catgcctggt ggacaaggaa attttttaag
     3001 agactttgca ccttttgggt agtgatttac cagagagaaa ggtctgtttt caaaaaagat
     3061 gctataatga attttatgtg tggcttgttt cttaaaagag aatttaactt attgttttta
     3121 ttgtatggtc acatatgctg ataatctgct attattcatt gttttctaga aagtgaaata
     3181 agtatttaag agcttaaggc cccatgcttt gctcctggga cccatccgcg atgcatgctg
     3241 agctatgtgt gtttttaatt ttacactgct ctttcctgcc tgtttgtttt aatggttatt
     3301 gcaggaatta aggtacatgt ctcttggttt tatgtaatat ggcactttat aaaatagtat
     3361 gagtattgca gtctgtctta gaaggtgcac tgtgtcatag gtactgtatt ttaccattct
     3421 tcttcttatt gactttaatt tgtcctcaca tctctcttct gctttctatg cagcaagtag
     3481 aaagggacgt gttgtctgat gactcgtttg ccacttacgc acccatccga gggaaagtaa
     3541 agggaattct attcaacact gtgcttcaca tctgtacact gtgttgccct acagcgagcc
     3601 agctcctcct gtaggcagag attatgtaca taatatttta gaaccataac ctatacttgt
     3661 tttgaaatgt ttctgttaaa ttttaaatcc acagagcatt ttataaactt atgcagagca
     3721 cttttattgc tcaaacgttc tgaattcata cagacaagtg ctatgccgtg aagatatttc
     3781 attgaaagac tgctgcattt taaagtacaa actaattaat tccctatgca aaaaaggaag
     3841 tcataggatt tgtatgttgt ttttttttct ttaaagccat tacatggaat gtcaagactg
     3901 agaaaaatga tcttcactat gtttacaaga actgtgctta aaaggatagt gtctaacatg
     3961 tttatttgac agttgtgtta atgataacct cgttagcatt agtttggaat ttggacacta
     4021 tacctattta tctctttctt aaggtaatag cagcattaat ttcaatctgt gatgaatact
     4081 aaaaataact gcattctgtg taatggtaat atatttatta ctaagtcaat gtacatatta
     4141 atagtatagg caacaaaatg gcaaatatta aaatattttc aagatattat attatcctgt
     4201 tcactttttg ttcacagtaa ttactgggga tattttttga tactctgact ggccaaatgg
     4261 ctatataatt cctcaatgag tcaataaaaa ttttatacta ctagctttgg gtcatttcta
     4321 gtaatgatag gaaactgtgt gaattactaa taaaatgtag ctgtttcaca aaaatgtgat
     4381 gcccttgggc agaggttttt aataagaata tgtccactta tttttacact gggtatatct
     4441 aggttatatt tggaaatgta taagttagtt cttgaagtta tttgtaaatg tgtatcctgt
     4501 aggaaatatg agttacaaag tacaaactaa ggagactgtt gccaacagaa agtatccaga
     4561 aaaggggttc aattccctca tgattgatta ggtcattgtg aatattggaa attgatgggt
     4621 tcttcatttc ttcacctggt atcttctgga ggattccata gtcactgaaa caggcaaaag
     4681 acaggtagag taagaagagg aagcttgctg tctgtgaact ggtaagactg attgaatctt
     4741 ctacagggag cttggaaaag attcaggaaa cctcaacaag atgctgttgc tgtcactgtc
     4801 actggtgtag aagtgcttga aaggcagtgt ggaatgatcc cttgcagatt ctgttagtgg
     4861 acagcaaggc tcctttagaa accacagaac ctctagttgc tcacatcaaa atgaaacaat
     4921 agaaatgcta catacaaaaa tactaagtga ggggctggag agatggcagc aattaagaac
     4981 aggcactgtt cttggcagaa ggatgtgagt tccgttccca gcatccatgt cacccagctc
     5041 tcaacaagcc tggagttgca gctccagagc atcccatgcg tcttcctgtg cacagaaata
     5101 ttaagtgaat agaagtaggt gagagaaaaa taaatctctg ctttaagttc cttttcttga
     5161 caattgtttt ggctgtagag tcaaattatc ctaccagtct ttattgatta cattgtatgt
     5221 aatttatgcc actgtatatc ccgagtcaca catggtgtaa ttgtgtatgt ctaatatatt
     5281 gaaaggcaag gatatgagag gggaacaaaa ctaaagtgga gagctttctt gtaataacta
     5341 tgccagatcc tgttgctgaa tgaactaatt tagctctctt gaaatccatc ggttcttcca
     5401 aaccgctata aaaaccattt aactattgga atgaagtgaa tttcaacacg attatctgtc
     5461 ttgaagttgc aggctcatgt gtgcatgaaa actgcctttt tcttttgaga ttaactgcta
     5521 attaagtaat tatgcaaatg gctgtatttt aaatgtaagg tggatatttt gaagaattct
     5581 actagggtaa caatgagtga tttgtttgtt tgttttttaa taaagaaaat cctttatcta
     5641 aaaaaaaaaa aaaaaaa
//
