LOCUS       NM_026653               3058 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus replication protein A1 (Rpa1), transcript variant 2,
            mRNA.
ACCESSION   NM_026653
VERSION     NM_026653.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Hayran,A.B., Liabakk,N.B., Aas,P.A., Kusnierczyk,A., Vagbo,C.B.,
            Sarno,A., Iveland,T.S., Chawla,K., Zahn,A., Di Noia,J.M.,
            Slupphaug,G. and Kavli,B.
  TITLE     RPA guides UNG to uracil in ssDNA to facilitate antibody class
            switching and repair of mutagenic uracil at the replication fork
  JOURNAL   Nucleic Acids Res 52 (2), 784-800 (2024)
   PUBMED   38000394
  REMARK    GeneRIF: RPA guides UNG to uracil in ssDNA to facilitate antibody
            class switching and repair of mutagenic uracil at the replication
            fork.
REFERENCE   3  (bases 1 to 3058)
  AUTHORS   Huang,T., Wu,X., Wang,S., Bao,Z., Wan,Y., Wang,Z., Li,M., Yu,X.,
            Lv,Y., Liu,Z., Chen,X., Chan,W.Y., Gao,F., Lu,G., Chen,Z.J. and
            Liu,H.
  TITLE     SPIDR is required for homologous recombination during mammalian
            meiosis
  JOURNAL   Nucleic Acids Res 51 (8), 3855-3868 (2023)
   PUBMED   36938872
REFERENCE   4  (bases 1 to 3058)
  AUTHORS   Song,J., Zhang,X., Yin,Y., Guo,M., Zhao,X., Wang,L., Ren,C.,
            Yin,Y., Zhang,X., Deng,X. and Lu,D.
  TITLE     Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates
            Inflammatory Damage through Triggering T Cell Necroptosis
  JOURNAL   Adv Sci (Weinh) 10 (11), e2206344 (2023)
   PUBMED   36721037
  REMARK    GeneRIF: Loss of RPA1 Impairs Peripheral T Cell Homeostasis and
            Exacerbates Inflammatory Damage through Triggering T Cell
            Necroptosis.
REFERENCE   5  (bases 1 to 3058)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3058)
  AUTHORS   Lipkin,S.M., Moens,P.B., Wang,V., Lenzi,M., Shanmugarajah,D.,
            Gilgeous,A., Thomas,J., Cheng,J., Touchman,J.W., Green,E.D.,
            Schwartzberg,P., Collins,F.S. and Cohen,P.E.
  TITLE     Meiotic arrest and aneuploidy in MLH3-deficient mice
  JOURNAL   Nat Genet 31 (4), 385-390 (2002)
   PUBMED   12091911
REFERENCE   7  (bases 1 to 3058)
  AUTHORS   Sutherland,H.G., Mumford,G.K., Newton,K., Ford,L.V., Farrall,R.,
            Dellaire,G., Caceres,J.F. and Bickmore,W.A.
  TITLE     Large-scale identification of mammalian proteins localized to
            nuclear sub-compartments
  JOURNAL   Hum Mol Genet 10 (18), 1995-2011 (2001)
   PUBMED   11555636
REFERENCE   8  (bases 1 to 3058)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 3058)
  AUTHORS   Hamilton,B.A., Smith,D.J., Mueller,K.L., Kerrebrock,A.W.,
            Bronson,R.T., van Berkel,V., Daly,M.J., Kruglyak,L., Reeve,M.P.,
            Nemhauser,J.L., Hawkins,T.L., Rubin,E.M. and Lander,E.S.
  TITLE     The vibrator mutation causes neurodegeneration via reduced
            expression of PITP alpha: positional complementation cloning and
            extragenic suppression
  JOURNAL   Neuron 18 (5), 711-722 (1997)
   PUBMED   9182797
REFERENCE   10 (bases 1 to 3058)
  AUTHORS   Dear,T.N., Hainzl,T., Follo,M., Nehls,M., Wilmore,H., Matena,K. and
            Boehm,T.
  TITLE     Identification of interaction partners for the
            basic-helix-loop-helix protein E47
  JOURNAL   Oncogene 14 (8), 891-898 (1997)
   PUBMED   9050988
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ086677.1, CJ078143.1, AK148035.1 and BE981198.1.
            
            On Aug 15, 2009 this sequence version replaced NM_026653.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. The resulting
            isoform (2) lacks an alternate segment, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035084.1, SRR1660817.149113.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                CJ086677.1         1-41
            42-60               CJ078143.1         42-60
            61-2717             AK148035.1         2-2658
            2718-3058           BE981198.1         1-341               c
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.79 cM"
     gene            1..3058
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /note="replication protein A1"
                     /db_xref="GeneID:68275"
                     /db_xref="MGI:MGI:1915525"
     exon            1..158
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1997
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /note="isoform 2 is encoded by transcript variant 2;
                     replication protein A 70 kDa DNA-binding subunit; RP-A
                     p70; RF-A protein 1; replication factor A protein 1"
                     /codon_start=1
                     /product="replication protein A 70 kDa DNA-binding subunit
                     isoform 2"
                     /protein_id="NP_080929.1"
                     /db_xref="CCDS:CCDS25044.1"
                     /db_xref="GeneID:68275"
                     /db_xref="MGI:MGI:1915525"
                     /translation="MVGHLSEGAIEVMIQQENTSIKPILQVINIRPISTGNRSPRYRL
                     LMSDGLNTLSSFMLATQLNTLVEGGQLASNCVCQVHKFIVNTLKDGRKVVVLMDLEVM
                     KSAEDVGLKIGNPVPYNEGYGQQQQQQQQQQQQAVPSPASAATPPASKPQPQNGSLGM
                     GSTAAKAYGASKPFGKPAGTGLLQPSGGTQSKVVPIASLTPYQSKWTICARVTNKSQI
                     RTWSNSRGEGKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGALKIANK
                     QFSAVKNDYEMTFNNETSVLPCEDGHHLPTVQFDFTGIGDLESKAKDALVDIIGICKS
                     YEDSIKITVKSNNREVAKRNIYLMDMSGKVVTTTLWGEDADKFDGSRQPVMAIKGARV
                     SDFGGRSLSVLSSSTVIVNPDIPEAYKLRGWFDSEGQALDGVSISDHRSGGAGGGNTN
                     WKTLHEAKSENLGQGDKADYFSTVAAVVFLRKENCMYQACPTQDCNKKVIDQQNGLYR
                     CEKCDREFPNFKYRMILSANIADFQENQWVTCFQESAEAILGQNTMYLGELKEKNEQA
                     FEEVFQNANFRSFTFRIRVKLETYNDESRIKATVMDVKPVDFRDYGRRLIANIRKNM"
     exon            159..209
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            210..288
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            289..397
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            398..486
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            487..606
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            607..739
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            740..842
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            843..911
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1104
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1244
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1393
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1526
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1703
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1811
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1898
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..3043
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2927..2932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /note="hexamer: AATAAA"
     polyA_site      2954
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /note="major polyA site"
     regulatory      3010..3015
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
                     /note="hexamer: AATAAA"
     polyA_site      3030
                     /gene="Rpa1"
                     /gene_synonym="5031405K23Rik; 70kDa; RF-A; RP-A; Rpa"
ORIGIN      
        1 gcgctcacgc tgggggcgga tcggcacgca cagttgctct agccaatcgt tgagcagccc
       61 gcgggacccc ggcagagaag ctcaggcggt gcaagagcgc gagcgcggga gttagcggtg
      121 cagccatggt gggacacctg agcgaggggg ccatcgaggt catgatacag caggaaaata
      181 catccataaa gcccatcctg caggtcatta acatccgtcc catttctaca gggaataggt
      241 caccccggta ccgactgctc atgagtgatg ggttgaacac gctttcctcg ttcatgctgg
      301 ccacgcagct gaacaccctg gtcgagggag ggcagctggc cagcaactgt gtctgccagg
      361 tccacaagtt catcgtcaac accctgaaag acggcaggaa agtagttgtt ttgatggact
      421 tggaagttat gaaatctgct gaagacgttg gattaaagat tgggaatcca gtgccctata
      481 atgaaggata tgggcagcag cagcagcagc agcagcagca gcagcagcaa gcagtccctt
      541 ccccagcctc tgcagcgaca ccaccagcca gcaagcccca gccacagaat gggagtctgg
      601 gaatgggttc cactgcagct aaggcttatg gtgcctcaaa gccatttgga aaacctgccg
      661 gaactggcct gctgcagcct tcaggtggga cacagtccaa agtggtgccc atcgccagcc
      721 tcaccccgta ccagtccaag tggactattt gtgcccgtgt caccaacaaa agtcagatcc
      781 gcacctggag taattcccgg ggggaaggaa agcttttctc tctagaactt gtggatgaaa
      841 gcggtgaaat cagagctact gctttcaatg agcaagtgga caagttcttt ccccttattg
      901 aagtgaacaa ggtgtattac ttctcaaagg gcgccctgaa gatcgctaac aaacagttct
      961 ccgctgttaa aaatgactat gagatgacct tcaataatga gacttctgtc cttccctgtg
     1021 aagatggcca tcacttaccc acagttcagt ttgatttcac agggatcggt gacctagaga
     1081 gcaaggctaa agacgcacta gtagacatca ttggaatctg caagagctat gaagattcga
     1141 ttaaaatcac agtgaagtcg aacaacagag aagttgctaa gagaaatatc tatttgatgg
     1201 acatgtcagg gaaggtggtg accacaactc tgtggggaga agatgctgac aagtttgacg
     1261 gctctcggca gcccgtgatg gccatcaaag gagcccgagt ctctgacttc ggtggacgga
     1321 gcctctcggt cctctcatcc agcactgtca ttgtgaatcc tgacatccca gaggcctata
     1381 agcttcgtgg ctggtttgac tcagaaggac aagccttaga tggtgtttcc atctctgacc
     1441 acaggagtgg aggggccgga gggggcaaca ccaactggaa aactttgcat gaggctaaat
     1501 ctgagaacct gggccaggga gacaaggcgg actatttcag cactgtggca gcggtggtgt
     1561 ttctccgcaa agagaactgt atgtaccagg cctgcccaac ccaggactgc aataagaaag
     1621 tgattgacca gcagaatggg ttgtaccgct gtgagaagtg tgaccgggaa tttcccaatt
     1681 tcaaataccg catgatctta tcggcaaata ttgcagattt tcaagagaac cagtgggtga
     1741 cgtgtttcca ggagtctgca gaggccatcc ttggacagaa cactatgtac cttggggaac
     1801 tcaaagagaa gaacgagcag gcgtttgaag aggttttcca gaatgccaac ttccggtcat
     1861 ttacgttcag aatcagggtc aagctggaga cctacaatga tgaatctcga attaaggcca
     1921 ctgtgatgga cgtgaagccc gtggacttca gagactatgg cagacggctg atcgcgaaca
     1981 tcaggaagaa catgtgatgg gagcaagtac cactggtggc cagaacggaa gagagaagaa
     2041 cagaaataga atggcttcct tcccgcccca tgggacagct ccacaatgaa gatcctctag
     2101 gggaggctgg caacccatct gggcccctct tgggactcac tgataagcaa agacagtgag
     2161 ctcagagggc tgtgtgtgaa ccttccgctc cagttctcag ccaccggtag tgtggcagca
     2221 gtcccctcct cctgccttcc ggctttgtcg gatttcatga aattttgtta tcttcaaaca
     2281 gaaattatgt cacaagtcat taaccccact ctggataatg aggcaaatta tgtaacctgg
     2341 agtgtgcttt tccagtggtg gcaccttttt gtcttgagtt cctcctgggg ggagaagagt
     2401 aggggagaac ccaggtgttc agagggtgcg tctgcctgga atcaaagggt ctgttttgca
     2461 gagttactgc atggttgtta atgcttagaa tggcaacaag gtagaattat taatcaaagg
     2521 cctatcgtta atatctgaat atccttcctt cagggtttga taggtttaat agactgagtc
     2581 agatgactct gatattaatc agaattgtct cctctgagga cggctgataa gcactggctt
     2641 gctgtcccct tggggaaaag caaaccccca aattgcataa tttacttcat tatccacata
     2701 gtggggtcag ttacttgaga cacaatccct gtttgaagat aacagaatct tagtaaatcc
     2761 gacaaagcct gcagacagag gtggcactgg gacatccagg ctgctacaga gcatggcttc
     2821 agtttgcatc agttaccgtt cggttgccag gcggacctgt gtacctttat taaagctatg
     2881 ttaaatagaa tttaagaaag aagctttgtt tcctttgctt tgtttgaata aacagtatat
     2941 ctttgtggat cctactttct ttgactgtaa aggattcttg taactggaaa gcaattaatt
     3001 agccttcata ataaatattc actagggaaa gatgttaact cataaaaaaa aaaaaaaa
//
