LOCUS       NM_145852                910 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ropporin 1-like (Ropn1l), mRNA.
ACCESSION   NM_145852 XM_909884
VERSION     NM_145852.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 910)
  AUTHORS   Zhang,X., Xiao,Z., Zhang,J., Xu,C., Liu,S., Cheng,L., Zhou,S.,
            Zhao,S., Zhang,Y., Wu,J., Wang,Y. and Liu,M.
  TITLE     Differential requirements of IQUB for the assembly of radial spoke
            1 and the motility of mouse cilia and flagella
  JOURNAL   Cell Rep 41 (8), 111683 (2022)
   PUBMED   36417862
REFERENCE   3  (bases 1 to 910)
  AUTHORS   Liu,S., Zhang,J., Kherraf,Z.E., Sun,S., Zhang,X., Cazin,C.,
            Coutton,C., Zouari,R., Zhao,S., Hu,F., Fourati Ben Mustapha,S.,
            Arnoult,C., Ray,P.F. and Liu,M.
  TITLE     CFAP61 is required for sperm flagellum formation and male fertility
            in human and mouse
  JOURNAL   Development 148 (23) (2021)
   PUBMED   34792097
REFERENCE   4  (bases 1 to 910)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 910)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 910)
  AUTHORS   Zhang,X., Chen,M., Yu,R., Liu,B., Tian,Z. and Liu,S.
  TITLE     FSCB phosphorylation regulates mouse spermatozoa capacitation
            through suppressing SUMOylation of ROPN1/ROPN1L
  JOURNAL   Am J Transl Res 8 (6), 2776-2782 (2016)
   PUBMED   27398160
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 910)
  AUTHORS   Fiedler,S.E., Dudiki,T., Vijayaraghavan,S. and Carr,D.W.
  TITLE     Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine
            sperm motility, phosphorylation, and fibrous sheath integrity
  JOURNAL   Biol Reprod 88 (2), 41 (2013)
   PUBMED   23303679
  REMARK    GeneRIF: Mutations in ROPN1 and ROPN1L can cause defects in fibrous
            sheath integrity, sperm motility, and PKA-dependent signaling
            processes, leading to male infertility.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 910)
  AUTHORS   Fiedler,S.E., Sisson,J.H., Wyatt,T.A., Pavlik,J.A., Gambling,T.M.,
            Carson,J.L. and Carr,D.W.
  TITLE     Loss of ASP but not ROPN1 reduces mammalian ciliary motility
  JOURNAL   Cytoskeleton (Hoboken) 69 (1), 22-32 (2012)
   PUBMED   22021175
  REMARK    GeneRIF: The beat frequency of cilia from mice lacking ASP is
            significantly slower than wild type, indicating that ASP signaling
            may regulate ciliary motility.
REFERENCE   9  (bases 1 to 910)
  AUTHORS   Anway,M.D., Ravindranath,N., Dym,M. and Griswold,M.D.
  TITLE     Identification of a murine testis complementary DNA encoding a
            homolog to human A-kinase anchoring protein-associated sperm
            protein
  JOURNAL   Biol Reprod 66 (6), 1755-1761 (2002)
   PUBMED   12021058
  REMARK    GeneRIF: Northern blot analysis of testis RNA from 5-, 15-, 25-,
            and 40-day-old mice showed expression of the ASP gene and in situ
            hybridization analysis localized the ASP mRNA to the germ cell
            compartment of the seminiferous tubules in the testis
REFERENCE   10 (bases 1 to 910)
  AUTHORS   Kerr,S.M., Taggart,M.H., Lee,M. and Cooke,H.J.
  TITLE     Ott, a mouse X-linked multigene family expressed specifically
            during meiosis
  JOURNAL   Hum Mol Genet 5 (8), 1139-1148 (1996)
   PUBMED   8842733
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK131675.1 and AV039619.2.
            
            On Nov 19, 2006 this sequence version replaced NM_145852.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BB667161.1, AK131675.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-906               AK131675.1         50-955
            907-910             AV039619.2         268-271
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 12.94 cM"
     gene            1..910
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /note="ropporin 1-like"
                     /db_xref="GeneID:252967"
                     /db_xref="MGI:MGI:2182357"
     exon            1..277
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..803
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /note="AKAP-associated sperm protein; A-kinase anchoring
                     protein-associated sperm protein"
                     /codon_start=1
                     /product="ropporin-1-like protein"
                     /protein_id="NP_665851.2"
                     /db_xref="CCDS:CCDS37053.1"
                     /db_xref="GeneID:252967"
                     /db_xref="MGI:MGI:2182357"
                     /translation="MPLPDTMFCAQQIHIPPELPDILKQFTKAAIRTQPADVLQWSAG
                     YFSALSRGDPLPVKDRIEMPVATQKTDTGLTQGLLKVLHKQCSHKQYVELADLEKKWK
                     NLCLPVEKLRTILELDPCEDKIEWIKFLALGCSSLGRTLNTAMKNVCEILTSDPEGGP
                     ARIPFETFAYVYQYLSGLDPELPAVETENYLTSLRLMSESRKNGMIGLSDFFVGKKII
                     "
     exon            278..401
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            402..563
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            564..739
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            740..910
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      888..893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /note="hexamer: AATACA"
     polyA_site      910
                     /gene="Ropn1l"
                     /gene_synonym="Akapasp; ASP"
                     /note="major polyA site"
ORIGIN      
        1 cacaatcgcg tgctctatcc gctctgggcg gttgggcctc agtttcctga gggggcggag
       61 cagtccctcc ccgccgcccg ctgcgctgac cctgcctctc ctccccgcag cacaacgtcc
      121 caggccgccg cggtctcccc agaacgatgc cgctgcccga caccatgttc tgcgcgcagc
      181 agatccacat ccccccggag ctgccggaca tcctcaagca gttcaccaag gctgccatcc
      241 gtacgcagcc ggcggacgtg ctgcagtggt cggcggggta tttttcagcc ctgtccagag
      301 gagacccact tcctgtaaag gacagaattg aaatgcctgt ggccacacag aagactgaca
      361 caggcctgac ccagggactc ctgaaagtcc tgcacaagca gtgtagccac aagcaatatg
      421 tggagttagc agatcttgag aagaagtgga aaaatttgtg cctgccagta gaaaagctca
      481 gaacgatcct ggagctggat ccatgtgagg acaaaataga gtggatcaag tttttagcgc
      541 tcggatgcag ctccctgggc aggaccctga acacggccat gaagaacgtg tgtgagatcc
      601 tcacctctga cccggagggc gggcctgctc gcatcccctt tgaaaccttt gcctatgttt
      661 accagtactt gtctggactg gacccagaac tcccggccgt ggagaccgag aactatctta
      721 ccagtttaag actgatgtca gaatctagaa agaatggcat gataggactt tcggacttct
      781 tcgttggaaa aaagataata tagaaagcct tgaagagaaa gctctggaag cagagcattg
      841 atacttgttt aatgttgaag ccatgcttag gagaaacctt ggcgccaaat acagcttgtc
      901 attacctgtg
//
