LOCUS       NM_001418955             900 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus ropporin, rhophilin associated protein 1 (Ropn1),
            transcript variant 2, mRNA.
ACCESSION   NM_001418955 XM_006522698
VERSION     NM_001418955.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Liu,S., Zhang,J., Kherraf,Z.E., Sun,S., Zhang,X., Cazin,C.,
            Coutton,C., Zouari,R., Zhao,S., Hu,F., Fourati Ben Mustapha,S.,
            Arnoult,C., Ray,P.F. and Liu,M.
  TITLE     CFAP61 is required for sperm flagellum formation and male fertility
            in human and mouse
  JOURNAL   Development 148 (23) (2021)
   PUBMED   34792097
REFERENCE   2  (bases 1 to 900)
  AUTHORS   Zhang,X., Chen,M., Yu,R., Liu,B., Tian,Z. and Liu,S.
  TITLE     FSCB phosphorylation regulates mouse spermatozoa capacitation
            through suppressing SUMOylation of ROPN1/ROPN1L
  JOURNAL   Am J Transl Res 8 (6), 2776-2782 (2016)
   PUBMED   27398160
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 900)
  AUTHORS   Fiedler,S.E., Dudiki,T., Vijayaraghavan,S. and Carr,D.W.
  TITLE     Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine
            sperm motility, phosphorylation, and fibrous sheath integrity
  JOURNAL   Biol Reprod 88 (2), 41 (2013)
   PUBMED   23303679
  REMARK    GeneRIF: Mutations in ROPN1 and ROPN1L can cause defects in fibrous
            sheath integrity, sperm motility, and PKA-dependent signaling
            processes, leading to male infertility.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 900)
  AUTHORS   Fiedler,S.E., Sisson,J.H., Wyatt,T.A., Pavlik,J.A., Gambling,T.M.,
            Carson,J.L. and Carr,D.W.
  TITLE     Loss of ASP but not ROPN1 reduces mammalian ciliary motility
  JOURNAL   Cytoskeleton (Hoboken) 69 (1), 22-32 (2012)
   PUBMED   22021175
REFERENCE   5  (bases 1 to 900)
  AUTHORS   Li,Y.F., He,W., Kim,Y.H., Mandal,A., Digilio,L., Klotz,K.,
            Flickinger,C.J. and Herr,J.C.
  TITLE     CABYR isoforms expressed in late steps of spermiogenesis bind with
            AKAPs and ropporin in mouse sperm fibrous sheath
  JOURNAL   Reprod Biol Endocrinol 8, 101 (2010)
   PUBMED   20731842
  REMARK    GeneRIF: CABYR expresses at the late stage of spermiogenesis and
            its isoforms oligomerize and bind with AKAPs and ropporin.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 900)
  AUTHORS   Li,Y.F., He,W., Jha,K.N., Klotz,K., Kim,Y.H., Mandal,A., Pulido,S.,
            Digilio,L., Flickinger,C.J. and Herr,J.C.
  TITLE     FSCB, a novel protein kinase A-phosphorylated calcium-binding
            protein, is a CABYR-binding partner involved in late steps of
            fibrous sheath biogenesis
  JOURNAL   J Biol Chem 282 (47), 34104-34119 (2007)
   PUBMED   17855365
REFERENCE   7  (bases 1 to 900)
  AUTHORS   Carr,D.W., Fujita,A., Stentz,C.L., Liberty,G.A., Olson,G.E. and
            Narumiya,S.
  TITLE     Identification of sperm-specific proteins that interact with
            A-kinase anchoring proteins in a manner similar to the type II
            regulatory subunit of PKA
  JOURNAL   J Biol Chem 276 (20), 17332-17338 (2001)
   PUBMED   11278869
REFERENCE   8  (bases 1 to 900)
  AUTHORS   Fujita,A., Nakamura,K., Kato,T., Watanabe,N., Ishizaki,T.,
            Kimura,K., Mizoguchi,A. and Narumiya,S.
  TITLE     Ropporin, a sperm-specific binding protein of rhophilin, that is
            localized in the fibrous sheath of sperm flagella
  JOURNAL   J Cell Sci 113 (Pt 1), 103-112 (2000)
   PUBMED   10591629
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154588.2 and AC154456.2.
            
            On Mar 24, 2023 this sequence version replaced XM_006522698.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.57830.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AC154588.2         6738-6858           c
            122-249             AC154456.2         131517-131644
            250-367             AC154456.2         134730-134847
            368-529             AC154456.2         135934-136095
            530-705             AC154456.2         141969-142144
            706-900             AC154456.2         143249-143443
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 24.44 cM"
     gene            1..900
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /note="ropporin, rhophilin associated protein 1"
                     /db_xref="GeneID:76378"
                     /db_xref="MGI:MGI:1923628"
     exon            1..121
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     exon            122..249
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..772
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /note="rhophilin-associated protein 1;
                     rhophilin-associated 'oppo' protein; rhophilin-associated
                     oppo protein"
                     /codon_start=1
                     /product="ropporin-1"
                     /protein_id="NP_001405884.1"
                     /db_xref="GeneID:76378"
                     /db_xref="MGI:MGI:1923628"
                     /translation="MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAM
                     SRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTD
                     LFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFST
                     FQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE"
     exon            250..367
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     exon            368..529
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     exon            530..705
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     exon            706..900
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      882..887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /note="hexamer: AATAAA"
     polyA_site      900
                     /gene="Ropn1"
                     /gene_synonym="1700008N21Rik; ODF6; RHPNAP1; ropporin;
                     Rppn"
                     /note="major polyA site"
ORIGIN      
        1 aggtccacag ctctcccccc accaagggtc caggcgttgg agctcctccc tcatctcccg
       61 cctgatacca ggcacgtgga gtcgcctgaa gccagacagc ccctttggag ggcaaactga
      121 ggtcaagcga tcaatgcctc agacagacaa gcaagtatgc atcccaccgg agctgccgga
      181 attgctgaag cagtttacca aagatgccat tcggacccag cctcctgatc tcatacagtg
      241 ggcagccgaa tatttcggag ccatgtcccg tggagagatt cctccagtga gagagaggtc
      301 tgaacaaatc cctttgtcca actgggctga gctgacacct gagctgctga aggtcctgca
      361 ttctcgggtt gctggcagac tgatcatcca cgcagatgag ctggcccaga tgtggaaggt
      421 gctgaatctc cccacggacc tgtttaacag tgtgatgaac gtgggtcgct tcacagagga
      481 gatcgagtgg ctgaagtttt tagcccttgc ttgtagctct cttggagtta ccattgccaa
      541 aactctcaag atagtgtgtg aagttttatc ctccgaccat gatggaggac caccccggat
      601 tcctttcagc accttccagt ttctgtacac gtatatcgct gaagtggatg gggaaatctc
      661 ttcatcccac gttagcagga tgttaaacta cattgaacag gaagtaattg gtcctgatgg
      721 cttaatcaag gtgaatgact ttactcaaaa ccctagggtt cggctggaat aaaagcacaa
      781 ttttggcaat tttaaaggaa gatacagagg tggctgtact tcagaatagc tgaacaccat
      841 gcaccatcta aagtcaattt tcttttacga ctggtacaac taataaacaa ttagccagga
//
