LOCUS       NM_001309390            3785 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus roundabout guidance receptor 4 (Robo4), transcript
            variant 1, mRNA.
ACCESSION   NM_001309390 XM_006510646
VERSION     NM_001309390.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Deng,F., Chen,C.B., Li,H., Huang,S., Xu,C. and Xiao,X.
  TITLE     CircSlc17a5 controlled by VLDLR/QKI pathway regulated the choroidal
            angiogenesis
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (7), 119802 (2024)
   PUBMED   39069227
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Wang,L., Chen,Y., Wu,H., Yu,H.H. and Ma,L.
  TITLE     Slit2-Robo4 signal pathway and tight junction in intestine mediate
            LPS-induced inflammation in mice
  JOURNAL   Eur J Med Res 29 (1), 349 (2024)
   PUBMED   38937814
  REMARK    GeneRIF: Slit2-Robo4 signal pathway and tight junction in intestine
            mediate LPS-induced inflammation in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3785)
  AUTHORS   Tanaka,M., Shirakura,K., Takayama,Y., Muatsui,M., Watanabe,Y.,
            Yamamoto,T., Takahashi,J., Tanaka,S., Hino,N., Doi,T., Obana,M.,
            Fujio,Y., Takayama,K. and Okada,Y.
  TITLE     Endothelial ROBO4 suppresses PTGS2/COX-2 expression and
            inflammatory diseases
  JOURNAL   Commun Biol 7 (1), 599 (2024)
   PUBMED   38762541
  REMARK    GeneRIF: Endothelial ROBO4 suppresses PTGS2/COX-2 expression and
            inflammatory diseases.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3785)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3785)
  AUTHORS   Adzraku,S.Y., Wang,G., Cao,C., Bao,Y., Wang,Y., Smith,A.O., Du,Y.,
            Wang,H., Li,Y., Xu,K., Qiao,J., Ju,W. and Zeng,L.
  TITLE     Robo4 inhibits gamma radiation-induced permeability of a murine
            microvascular endothelial cell by regulating the junctions
  JOURNAL   Cell Mol Biol Lett 28 (1), 2 (2023)
   PUBMED   36647012
  REMARK    GeneRIF: Robo4 inhibits gamma radiation-induced permeability of a
            murine microvascular endothelial cell by regulating the junctions.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3785)
  AUTHORS   Hardie,W.D., Korfhagen,T.R., Sartor,M.A., Prestridge,A.,
            Medvedovic,M., Le Cras,T.D., Ikegami,M., Wesselkamper,S.C.,
            Davidson,C., Dietsch,M., Nichols,W., Whitsett,J.A. and Leikauf,G.D.
  TITLE     Genomic profile of matrix and vasculature remodeling in TGF-alpha
            induced pulmonary fibrosis
  JOURNAL   Am J Respir Cell Mol Biol 37 (3), 309-321 (2007)
   PUBMED   17496152
  REMARK    GeneRIF: Robo4 mRNA level decreases during vascular decline in
            pulmonary fibrosis.
REFERENCE   7  (bases 1 to 3785)
  AUTHORS   Okada,Y., Yano,K., Jin,E., Funahashi,N., Kitayama,M., Doi,T.,
            Spokes,K., Beeler,D.L., Shih,S.C., Okada,H., Danilov,T.A.,
            Maynard,E., Minami,T., Oettgen,P. and Aird,W.C.
  TITLE     A three-kilobase fragment of the human Robo4 promoter directs cell
            type-specific expression in endothelium
  JOURNAL   Circ Res 100 (12), 1712-1722 (2007)
   PUBMED   17495228
REFERENCE   8  (bases 1 to 3785)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 3785)
  AUTHORS   Park,K.W., Morrison,C.M., Sorensen,L.K., Jones,C.A., Rao,Y.,
            Chien,C.B., Wu,J.Y., Urness,L.D. and Li,D.Y.
  TITLE     Robo4 is a vascular-specific receptor that inhibits endothelial
            migration
  JOURNAL   Dev Biol 261 (1), 251-267 (2003)
   PUBMED   12941633
  REMARK    GeneRIF: Robo4 is a bona fide member of the Robo family and may
            provide a repulsive cue to migrating endothelial cells during
            vascular development.
REFERENCE   10 (bases 1 to 3785)
  AUTHORS   Huminiecki,L., Gorn,M., Suchting,S., Poulsom,R. and Bicknell,R.
  TITLE     Magic roundabout is a new member of the roundabout receptor family
            that is endothelial specific and expressed at sites of active
            angiogenesis
  JOURNAL   Genomics 79 (4), 547-552 (2002)
   PUBMED   11944987
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138284.9.
            
            On May 20, 2019 this sequence version replaced NM_001309390.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF536772.1, SRR1660815.250070.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               AC138284.9         187733-187912
            181-513             AC138284.9         188316-188648
            514-671             AC138284.9         188913-189070
            672-792             AC138284.9         189204-189324
            793-920             AC138284.9         189835-189962
            921-1149            AC138284.9         190054-190282
            1150-1262           AC138284.9         190532-190644
            1263-1431           AC138284.9         191334-191502
            1432-1617           AC138284.9         191659-191844
            1618-1660           AC138284.9         191950-191992
            1661-1797           AC138284.9         193699-193835
            1798-2061           AC138284.9         193934-194197
            2062-2169           AC138284.9         196143-196250
            2170-2329           AC138284.9         196440-196599
            2330-2572           AC138284.9         196741-196983
            2573-2931           AC138284.9         197106-197464
            2932-3138           AC138284.9         198790-198996
            3139-3785           AC138284.9         199138-199784
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 20.76 cM"
     gene            1..3785
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /note="roundabout guidance receptor 4"
                     /db_xref="GeneID:74144"
                     /db_xref="MGI:MGI:1921394"
     exon            1..180
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..3149
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Magic roundabout; roundabout homolog 4"
                     /codon_start=1
                     /product="roundabout homolog 4 isoform 1 precursor"
                     /protein_id="NP_001296319.2"
                     /db_xref="CCDS:CCDS80976.1"
                     /db_xref="GeneID:74144"
                     /db_xref="MGI:MGI:1921394"
                     /translation="MGSGGTGLLGTEWPLPLLLLFIMGGEALDSPPQILVHPQDQLLQ
                     GSGPAKMRCRSSGQPPPTIRWLLNGQPLSMATPDLHYLLPDGTLLLHRPSVQGRPQDD
                     QNILSAILGVYTCEASNRLGTAVSRGARLSVAVLQEDFQIQPRDTVAVVGESLVLECG
                     PPWGYPKPSVSWWKDGKPLVLQPGRRTVSGDSLMVSRAEKNDSGTYMCMATNNAGQRE
                     SRAARVSIQESQDHKEHLELLAVRIQLENVTLLNPEPVKGPKPGPSVWLSWKVSGPAA
                     PAESYTALFRTQRSPRDQGSPWTEVLLRGLQSAKLGGLHWGQDYEFKVRPSSGRARGP
                     DSNVLLLRLPEQVPSAPPQGVTLRSGNGSVFVSWAPPPAESHNGVIRGYQVWSLGNAS
                     LPAANWTVVGEQTQLEIATRLPGSYCVQVAAVTGAGAGELSTPVCLLLEQAMEQSARD
                     PRKHVPWTLEQLRATLRRPEVIASSAVLLWLLLLGITVCIYRRRKAGVHLGPGLYRYT
                     SEDAILKHRMDHSDSPWLADTWRSTSGSRDLSSSSSLSSRLGLDPRDPLEGRRSLISW
                     DPRSPGVPLLPDTSTFYGSLIAEQPSSPPVRPSPKTPAARRFPSKLAGTSSPWASSDS
                     LCSRRGLCSPRMSLTPTEAWKAKKKQELHQANSSPLLRGSHPMEIWAWELGSRASKNL
                     SQSPGPNSGSPGEAPRAVVSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQ
                     CVEKLQAPSSDPLPAAPLSVLNSSRPSSPQASFLSCPSPSSSNLSSSSLSSLEEEEDQ
                     DSVLTPEEVALCLELSDGEETPTNSVSPMPRAPSPPTTYGYISIPTCSGLADMGRAGG
                     GVGSEVGNLLYPPRPCPTPTPSEGSLANGWGSASEDNVPSARASLVSSSDGSFLADTH
                     FARALAVAVDSFGLSLDPREADCVFTDASSPPSPRGDLSLTRSFSLPLWEWRPDWLED
                     AEISHTQRLGRGLPPWPPDSRASSQRSWLTGAVPKAGDSS"
     exon            181..513
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            514..671
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            672..792
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            793..920
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1149
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1262
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1431
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1617
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1660
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1797
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..2061
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2169
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2329
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2572
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2931
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..3138
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3785
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3767..3772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /note="hexamer: TATAAA"
     polyA_site      3785
                     /gene="Robo4"
                     /gene_synonym="1200012D01Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtcacaaac aactaccacc accccacccc tccctttctt cccttactgc tgtgagctca
       61 gaacaaccgg acaaaagtgt atgggacaag gagaggagcc gagagcagcc atgggctctg
      121 gaggaacggg cctcctgggg acggagtggc ctctgcctct gctgctgctt ttcatcatgg
      181 gaggtgaggc tctggattct ccaccccaga tcctagttca cccccaggac cagctacttc
      241 agggctctgg cccagccaag atgaggtgca gatcatccgg ccaaccacct cccactatcc
      301 gctggctgct gaatgggcag cccctcagca tggccacccc agacctacat taccttttgc
      361 cggatgggac cctcctgtta catcggccct ctgtccaggg acggccacaa gatgaccaga
      421 acatcctctc agcaatcctg ggtgtctaca catgtgaggc cagcaaccgg ctgggcacag
      481 cagtgagccg gggtgctagg ctgtctgtgg ctgtcctcca ggaggacttc cagatccaac
      541 ctcgggacac agtggccgtg gtgggagaga gcttggttct tgagtgtggt cctccctggg
      601 gctacccaaa accctcggtc tcatggtgga aagacgggaa acccctggtc ctccagccag
      661 ggaggcgcac agtatctggg gattccctga tggtgtcaag agcagagaag aatgactcgg
      721 ggacctatat gtgtatggcc accaacaatg ctgggcaacg ggagagccga gcagccaggg
      781 tgtctatcca ggaatcccag gaccacaagg aacatctaga gcttctggct gttcgcattc
      841 agctggaaaa tgtgaccctg ctaaaccccg aacctgtaaa aggtcccaag cctgggccat
      901 ccgtgtggct cagctggaag gtgagcggcc ctgctgcacc tgctgagtca tacacagctc
      961 tgttcaggac tcagaggtcc cccagggacc aaggatctcc atggacagag gtgctgctgc
     1021 gtggcttgca gagtgcaaag cttgggggtc tccactgggg ccaagactat gaattcaaag
     1081 tgagaccgtc ctccggccgg gctcgaggcc ctgacagcaa tgtgttgctc ctgaggctgc
     1141 ctgaacaggt gcccagtgcc ccacctcaag gagtgacctt aagatctggc aacggtagtg
     1201 tctttgtgag ttgggctcca ccacctgctg aaagccataa tggtgtcatc cgtggttacc
     1261 aggtctggag cctgggcaat gcctcattgc ctgctgccaa ctggaccgta gtgggtgaac
     1321 agacccagct ggagatcgcc acacgactgc caggctccta ttgtgtgcaa gtggctgcag
     1381 tcactggagc tggtgctgga gaactcagta cccctgtctg cctcctttta gagcaggcca
     1441 tggagcaatc agcacgagac cccaggaaac atgttccctg gaccctggaa cagctgaggg
     1501 ccaccttgag acgaccagaa gtcattgcca gtagtgctgt cctactctgg ttgctgctac
     1561 taggcattac tgtgtgtatc tacagacgac gcaaagctgg ggtgcacctg ggcccaggtc
     1621 tgtacagata caccagcgag gacgccattc taaaacacag gatggaccac agtgactccc
     1681 catggctggc agacacctgg cgttccacct ctggctctcg agacctgagc agcagcagca
     1741 gccttagtag tcggctggga ttggaccctc gggacccact agagggcagg cgctccttga
     1801 tctcctggga ccctcggagc cccggtgtac ccctgcttcc agacacgagc acgttttacg
     1861 gctccctcat tgcagagcag ccttccagcc ctccagtccg gccaagcccc aagacaccag
     1921 ctgctaggcg ctttccatcc aagttggctg gaacctccag cccctgggct agctcagata
     1981 gtctctgcag ccgcagggga ctctgttccc cacgcatgtc tctgacccct acagaggctt
     2041 ggaaggccaa aaagaagcag gaattgcacc aagctaacag ctccccactg ctccggggca
     2101 gccaccccat ggaaatctgg gcctgggagt tgggaagcag agcctccaag aacctttctc
     2161 aaagcccagg tccaaactct ggttccccag gagaagcgcc ccgagccgtg gtatcctggc
     2221 gtgctgtggg accacaactt caccgcaact ccagtgagct ggcatctcgt ccactccctc
     2281 caacacccct ttctcttcgt ggagcttcca gtcatgaccc acagagccag tgtgtggaga
     2341 agctccaagc tccctcctct gacccactgc cagcagcccc tctctccgtc ctcaactctt
     2401 ccagaccttc cagcccccag gcctctttcc tctcctgtcc tagcccatcc tccagcaacc
     2461 tgtccagctc ctcgctgtca tccttagagg aggaggagga tcaggacagc gtgctcaccc
     2521 ccgaggaggt agccctgtgt ctggagctca gtgatgggga ggagacaccc acgaacagtg
     2581 tatctcctat gccaagagct ccttccccgc caacaaccta tggctatatc agcataccaa
     2641 cctgctcagg actggcagac atgggcagag ctggcggggg cgtggggtct gaggttggga
     2701 acttactgta tccacctcgg ccctgcccca cccctacacc cagcgagggc tccctggcca
     2761 atggttgggg ctcagcttct gaggacaatg tccccagcgc cagggccagc ctggttagct
     2821 cttctgatgg ctccttcctc gctgatactc actttgctcg tgccctggca gtggctgtgg
     2881 atagctttgg cctcagtctg gatcccaggg aagctgactg tgtcttcact gatgcctcat
     2941 cacctccctc ccctcggggt gatctctccc tgacccgaag cttctctctg cctttgtggg
     3001 agtggaggcc agactggttg gaagatgctg agatcagcca cacccagagg ctggggaggg
     3061 ggctgcctcc ctggcctcct gattctaggg cctcttccca gcgaagttgg ctaactggtg
     3121 ctgtgcccaa ggctggtgat tcctcctgaa ttgtccctga gaaggccaga agagcaccca
     3181 gaccactctc ctgtctgtcc cctggctttc tcacatgtgg aggtcttggc ctatgcttct
     3241 ctgtaataga agtccaccgt cactaggctt ctggagagct ctgtcattgg gattgttaaa
     3301 ataaatgaaa gcaaaccaaa atatgatcac gggagtcttg gattcccact gagaacaaga
     3361 cagcatcttc aggacagcag actctccaca accagaacct ttggcctaag taagcctggc
     3421 tccggagctc ccacctaagt ggatcatgga aagaagggaa gccaaccagg tcttcaggaa
     3481 ggacagaaat gttttttggt gagggctatg gtggaggacc tgtggaagag ccctctcata
     3541 tctacttgga ctcctccctt agaggccagc tcaacccttt ccccagtcac accatgcaag
     3601 gaaactaaag gagaaaggtc gtggatgcag tgggccctat acagcgtcac agtcaatgct
     3661 tcaaagtgag atcaatggag gagactgaag gaaaggacgc agggaaacag ggaaccaatg
     3721 cgctattctc attctaccgc cactctgagc ttaaggaact taattctata aaactgtaaa
     3781 gacga
//
