LOCUS       NM_001170953            4853 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus RNA (guanine-7-) methyltransferase (Rnmt), transcript
            variant 2, mRNA.
ACCESSION   NM_001170953
VERSION     NM_001170953.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Young,K., Tscherner,A.K., Zhang,B., Meredith,M., McClatchie,T.,
            Trasler,J.M. and Baltz,J.M.
  TITLE     5,10-Methylenetetrahydrofolate reductase becomes phosphorylated
            during meiotic maturation in mouse oocytes
  JOURNAL   Zygote 30 (5), 674-688 (2022)
   PUBMED   35652653
REFERENCE   3  (bases 1 to 4853)
  AUTHORS   Galloway,A., Kaskar,A., Ditsova,D., Atrih,A., Yoshikawa,H.,
            Gomez-Moreira,C., Suska,O., Warminski,M., Grzela,R., Lamond,A.I.,
            Darzynkiewicz,E., Jemielity,J. and Cowling,V.H.
  TITLE     Upregulation of RNA cap methyltransferase RNMT drives ribosome
            biogenesis during T cell activation
  JOURNAL   Nucleic Acids Res 49 (12), 6722-6738 (2021)
   PUBMED   34125914
  REMARK    GeneRIF: Upregulation of RNA cap methyltransferase RNMT drives
            ribosome biogenesis during T cell activation.
REFERENCE   4  (bases 1 to 4853)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4853)
  AUTHORS   Nakahara,M., Tateyama,H., Araki,M., Nakagata,N., Yamamura,K. and
            Araki,K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   6  (bases 1 to 4853)
  AUTHORS   Ghai,R., Bugarcic,A., Liu,H., Norwood,S.J., Skeldal,S.,
            Coulson,E.J., Li,S.S., Teasdale,R.D. and Collins,B.M.
  TITLE     Structural basis for endosomal trafficking of diverse transmembrane
            cargos by PX-FERM proteins
  JOURNAL   Proc Natl Acad Sci U S A 110 (8), E643-E652 (2013)
   PUBMED   23382219
REFERENCE   7  (bases 1 to 4853)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   8  (bases 1 to 4853)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC111069.9, AK129130.1 and CO039099.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            lacks an in-frame exon in the 3' coding region, compared to variant
            3. This results in a shorter protein (isoform 2), compared to
            isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK015403.1, SRR7345562.2997814.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC111069.9         77909-77971
            64-1054             AK129130.1         1-991
            1055-4839           AK129130.1         1157-4941
            4840-4853           CO039099.1         9-22                c
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 41.22 cM"
     gene            1..4853
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /note="RNA (guanine-7-) methyltransferase"
                     /db_xref="GeneID:67897"
                     /db_xref="MGI:MGI:1915147"
     exon            1..71
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            72..491
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..1346
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /EC_number="2.1.1.56"
                     /note="isoform 2 is encoded by transcript variant 2; mRNA
                     cap guanine-N7 methyltransferase; mRNA cap
                     methyltransferase; mRNA (guanine-N(7)-)-methyltransferase;
                     mRNA cap guanine-N(7) methyltransferase"
                     /codon_start=1
                     /product="mRNA cap guanine-N(7) methyltransferase isoform
                     2"
                     /protein_id="NP_001164424.1"
                     /db_xref="CCDS:CCDS50314.1"
                     /db_xref="GeneID:67897"
                     /db_xref="MGI:MGI:1915147"
                     /translation="MEGSAKASVASDPESPPGGNEPAAASGQRLPENTPPCQQVDQPK
                     MQKEFGEDLVEQNSSYVQDSPSKKRKLDVEIILEEKHSEDDGGSAKRSKLERGDVSED
                     EPSLGRLNQTKRKLQPQDDEVPQKLQKLEEGHSSAVAAHYNELQEVGLAKRSQSRIFY
                     LRNFNNWIKSILIGEILEKVRQRKTRDITVLDLGCGKGGDLLKWRKGRISRLVCADIA
                     DISMKQCQQRYEDMRCRRDNEHIFSAEFITADCSKELLVEKFRDPEMYFDVCSCQFAC
                     HYSFESQVQADTMLRNACGRLNPGGYFIGTTPNSFELIMAKKYNMKLIYKKTFLEFYE
                     EKIKNNENKMLLKRMQALEQYPAHENSKLASEKVGDYTHAAEYLKKSQVRLPLGTLSK
                     SEWEATSIYLVFAFEKQQ"
     exon            492..633
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            634..759
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            760..872
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1054
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1172
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1274
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1308
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..4843
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1876..1881
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /note="hexamer: AATAAA"
     polyA_site      1898
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /note="major polyA site"
     regulatory      4823..4828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
                     /note="hexamer: AATAAA"
     polyA_site      4843
                     /gene="Rnmt"
                     /gene_synonym="2610002P10Rik; mKIAA0398; Rg7mt1"
ORIGIN      
        1 gaagtgacgt aacaaggcac gcgtggctcc ggccggcgtt tccgcaaaca gaatcgtgga
       61 tgactgtgtg gtgtcgattg atgaggtttt gctgtcagtt caagtaacga gaaatggaag
      121 gttccgcgaa agcgtcagtc gcttctgacc cagagtcccc tccaggtggt aatgagcccg
      181 ctgctgcctc tgggcagagg cttccagaaa atactcctcc atgccagcaa gtagatcagc
      241 caaaaatgca gaaggagttt ggggaggatc ttgtagagca aaattcgagt tatgtgcaag
      301 attctccatc caagaaaagg aaacttgatg ttgaaatcat cttagaggaa aagcactctg
      361 aggatgatgg aggttcggca aagagaagca aactggaaag aggtgatgtg tcggaagatg
      421 agccttccct tggtaggctt aaccagacca agagaaaact gcagcctcag gacgatgagg
      481 taccgcagaa gttgcagaag ctggaggaag gacacagctc agccgtggct gcccactata
      541 atgagcttca ggaagttggc ctggcgaagc gtagtcaaag tcgcattttt tacctaagaa
      601 acttcaataa ttggattaaa agtattctga ttggggaaat tttagaaaag gtacgacaaa
      661 gaaaaacccg tgacatcact gttttggacc tgggatgtgg aaaaggtgga gatttgctca
      721 agtggaggaa ggggagaatt agcaggctag tgtgtgctga tatcgctgat atttctatga
      781 aacaatgtca acagcgttac gaagacatga gatgtcgtcg cgataatgag catattttta
      841 gtgcagaatt tataactgct gactgttcaa aggaactttt ggttgaaaaa ttccgtgacc
      901 cagaaatgta ctttgatgtc tgcagctgcc agtttgcttg ccattactcc tttgagtccc
      961 aggtgcaggc ggatacgatg cttagaaatg cctgcgggag gcttaaccct ggcggctatt
     1021 ttattggcac cactcccaat agctttgaac tgataatggc aaagaagtat aatatgaaac
     1081 taatctacaa aaaaacattt ttggaattct atgaagaaaa gattaaaaac aatgaaaaca
     1141 aaatgctctt aaaacgaatg caggccctgg agcaataccc tgcacatgag aactctaaac
     1201 ttgcctctga aaaggtgggt gactacacgc atgcagcaga gtacctgaag aagagccaag
     1261 tgaggttacc tctgggaacc ttaagtaagt cagaatggga agctacaagt atctacttgg
     1321 tctttgcctt tgagaagcag cagtgagcac gttgctttag agcagcactc catcctccca
     1381 ggtggagcag accatctcag aaacatttga cagctgtttg tttattttaa tagtaagttc
     1441 tcaatgtgta ggatgctgcc acaaacttca gtgtatgaat ttgacactta ctgtctgtga
     1501 caggttagca taatgtgtgt acatagggat gagttgtctt gaagatctat ttttaagtac
     1561 tgttgtaatt gttcccctct actgtcaaaa ctctagcaag gcatgtcaga gcagctgacc
     1621 tccccagtgc tgtgatgtgt gagcagctga cctccccagt gctgggatgt gtgaatgtgt
     1681 ttacagagct gatttgacag tcgctagaat tggcagagga acgttcacgt ttccttacag
     1741 tgtttttcaa gatttaatgg attgtgttct gtatacttga atgtaagtgt tcaatatata
     1801 ttgctaaagt tataagatta aattttgatt ttagattgtc ctaagagcta cctggctaga
     1861 ttttgaccgt gtttcaataa aataactatt tgctaataca ctgcaattct gagactctat
     1921 agacgaactt agatggcagc acagagttag tgtggactgg ttggatgttc tctttagaat
     1981 ttcttaccgt agcaattagc atatctaatt actgaggttg tgattaaggt acgagtaact
     2041 tcactggaca gtgattccat ggctagattt ccttaaggtt ttgtgaaaat gccacgtcct
     2101 aattgtgttc cataatgttt tccgatggat gtgtgcacgt gcagtaggaa cagcacagtg
     2161 agatgcattt cagatgaatc ctcaccaata agacaactca ctcaccaatg ctgtatcctt
     2221 ccaggtccca acatgcagct cctgcccgtc tcacaacact gggactccct ccctaaggca
     2281 gaccccgctg tctaattgtc tttcagacat taactgttga gacgttaatg gtgtagtagt
     2341 cggcgtggct gccttccaag ggtttacttg ctgtcccaca gccatcatga ttgtcattgt
     2401 cagttagaga gccgtcttct agaactgagg ccacactgaa gaaacttgtc agcctttaca
     2461 tcaagaaact taagagcaaa tataattaga catttgttta aaaatcactt acttaaagga
     2521 caatttacaa agatgctact ttctgtaaaa atctatagta cttgctttta aaataggctc
     2581 agggccagag aggtggctca gtagttcaga gtgctggctg cgcgtggaga agacccaggt
     2641 tcagttccca gcacctacat gacagcttac aaccatcagt aactctagtt ccaggggatc
     2701 tgacatcatc ttttggcctc cctgggcacc aggcatgtgc atagtacata catataatgt
     2761 gcaggcagaa tacacatata aagtaaaaat aattacttaa aaaaattaaa gttataatct
     2821 agtttatata gtcactgttc ttagtattta gagggtagat ttggttctgg tgcgggggtt
     2881 gtgggttgtg ggtggatggg gaggaatgtt cttgctctta gaaattcagg ctcattttat
     2941 tcctggggaa gttccagaaa gtattttgat gatgtggcgt agtagagaaa gcttcgtcac
     3001 aattcatgat cttagttaac agttggtacg tcatttttag tctttttctg ccctttccat
     3061 tccatcccat gggtcatttt taggagagaa gaattcttca aagggtggaa taattgatgg
     3121 ttatcccaag cagcaagaag agaaatgtgg aggtcttggg cccttccctg aggactcaga
     3181 gaaaactggc ctgggtctga gatgggctct agtcattata cctactgtca aagacatagg
     3241 agagtttcca tgatgtcacc ctacagaaac agttcaagca gctgcctttt tccttttgtg
     3301 ttcctctctg ttgaacgtcc atccctccac ccctccttgc agaagttcag tgactcagga
     3361 tggattcttg aggacaagaa tgggaaggtg gtcttttcca ttgtttcaac aaataagact
     3421 ttgcccagca ttcttggaac ctaaatgttt aattttgtca tgacttgtag tacctgatga
     3481 gaaaggtgtg gcttttcatt gcagtatcca gaaagagtcc cttctcttgg gttcagtgat
     3541 gaaaatggtt cagatgggct gttcagatac gtctaggcct gtcacacagt gtatgctcag
     3601 gttgtttcca aatgaagatc aggggctgga gacagagatc tcaggttaag agtgcttgca
     3661 actttcagag gactttagtt tggttcccaa cacacatatc aagtggctta gaactacctg
     3721 tacctccaat taaaggtact tcagcactct cctttagtct ttgctggcac ccgcacatac
     3781 atgcagatac ctacacgtaa ataaaaaatc aaaagaagag catgtgggcc atttttggga
     3841 atgacacaac ttgaactaga ggtagttggc ttggggacaa agctgcacag cagtaggacc
     3901 tagaacatgg acttgtttgg gtctagaatt tactcgggaa cagtccagtg tatagtgttg
     3961 atggagagaa gctgaggagt tgtctgtccc tccccatcct tctggtaacc taacctgtac
     4021 agagagctgc aagcctgtca gagctggctc acacaccact tcagtgcccc tgagcacatt
     4081 agggactctt aacagcaagc aaagactaag tagagtcact cataagtagt ggaccctgtc
     4141 tgataactga gtggcagaga gaagatagag atttctcaaa aacagagccc tgttgaaact
     4201 tctactctgt agtgggctta aagtcgtatg ttcacacact tctcagggtg taacaggtgg
     4261 ttcctgcagc tttgagtgat acttactgag gccccttaac ttagatgtat taattcctga
     4321 gtaaggcgct ttagtacata aggtaccctg gcaggaatag agatagatta ttcttcacgg
     4381 tgtggagacc tggtcctcta accggacttg tgaaatgtat gcactagagg tgatggtgtt
     4441 cccccaacct tattgtgtgg atctatcagt gcaaaagagc ctcttaagag aagatgctgt
     4501 ctcagtgtga attaagtagg caggcctcat tatgtgaatt tgcttgctta aactctgttg
     4561 aagtgttgta tctatacagg cgatcaggga ttcttcacag acagaacata catgaatgca
     4621 gaagccagag gttccctttt gcctactatt tcttccacag ccaccatcca cttttaagca
     4681 aaatccattt catctggctt gaaatatgtg aaataaaata aaaaatacgt tccttggctt
     4741 tcactcggca gtgttcatga gactcttatt ctcaatagct gtacattgtt cattctcatg
     4801 attgtacagt attccgtgta tgaataaaca acattataca gtgaaaaaaa aaa
//
