LOCUS       NM_021419               2096 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ring finger protein 8 (Rnf8), mRNA.
ACCESSION   NM_021419
VERSION     NM_021419.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Krishnan,R., Lapierre,M., Gautreau,B., Nixon,K.C.J., El
            Ghamrasni,S., Patel,P.S., Hao,J., Yerlici,V.T., Guturi,K.K.N.,
            St-Germain,J., Mateo,F., Saad,A., Algouneh,A., Earnshaw,R.,
            Shili,D., Seitova,A., Miller,J., Khosraviani,N., Penn,A., Ho,B.,
            Sanchez,O., Hande,M.P., Masson,J.Y., Brown,G.W., Alaoui-Jamali,M.,
            Reynolds,J.J., Arrowsmith,C., Raught,B., Pujana,M.A., Mekhail,K.,
            Stewart,G.S., Hakem,A. and Hakem,R.
  TITLE     RNF8 ubiquitylation of XRN2 facilitates R-loop resolution and
            restrains genomic instability in BRCA1 mutant cells
  JOURNAL   Nucleic Acids Res 51 (19), 10484-10505 (2023)
   PUBMED   37697435
  REMARK    GeneRIF: RNF8 ubiquitylation of XRN2 facilitates R-loop resolution
            and restrains genomic instability in BRCA1 mutant cells.
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhu,X., Li,J., You,D., Xiao,Y., Huang,Z. and Yu,W.
  TITLE     Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic
            Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3beta
            Inhibition
  JOURNAL   Mol Neurobiol 59 (8), 4776-4790 (2022)
   PUBMED   35622272
  REMARK    GeneRIF: Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against
            Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent
            GSK3beta Inhibition.
            Erratum:[Mol Neurobiol. 2022 Aug;59(8):4791-4792. doi:
            10.1007/s12035-022-02938-9.. PMID: 35789977]
REFERENCE   3  (bases 1 to 2096)
  AUTHORS   Bonthuis,P.J., Steinwand,S., Stacher Horndli,C.N., Emery,J.,
            Huang,W.C., Kravitz,S., Ferris,E. and Gregg,C.
  TITLE     Noncanonical genomic imprinting in the monoamine system determines
            naturalistic foraging and brain-adrenal axis functions
  JOURNAL   Cell Rep 38 (10), 110500 (2022)
   PUBMED   35263575
REFERENCE   4  (bases 1 to 2096)
  AUTHORS   Abe,H., Meduri,R., Li,Z., Andreassen,P.R. and Namekawa,S.H.
  TITLE     RNF8 is not required for histone-to-protamine exchange in
            spermiogenesisdagger
  JOURNAL   Biol Reprod 105 (5), 1154-1159 (2021)
   PUBMED   34225362
  REMARK    GeneRIF: RNF8 is not required for histone-to-protamine exchange in
            spermiogenesisdagger.
REFERENCE   5  (bases 1 to 2096)
  AUTHORS   Zhu,Y., Shi,Y., Ke,X., Xuan,L. and Ma,Z.
  TITLE     RNF8 induces autophagy and reduces inflammation by promoting AKT
            degradation via ubiquitination in ulcerative colitis mice
  JOURNAL   J Biochem 168 (5), 445-453 (2020)
   PUBMED   32597970
  REMARK    GeneRIF: RNF8 induces autophagy and reduces inflammation by
            promoting AKT degradation via ubiquitination in ulcerative colitis
            mice.
REFERENCE   6  (bases 1 to 2096)
  AUTHORS   Gong,Z., Cho,Y.W., Kim,J.E., Ge,K. and Chen,J.
  TITLE     Accumulation of Pax2 transactivation domain interaction protein
            (PTIP) at sites of DNA breaks via RNF8-dependent pathway is
            required for cell survival after DNA damage
  JOURNAL   J Biol Chem 284 (11), 7284-7293 (2009)
   PUBMED   19124460
  REMARK    GeneRIF: subset of PTIP.PA1 complex is recruited to DNA damage
            sites via the RNF8-dependent pathway and is required for cell
            survival in response to DNA damage.
REFERENCE   7  (bases 1 to 2096)
  AUTHORS   Wu,J., Huen,M.S., Lu,L.Y., Ye,L., Dou,Y., Ljungman,M., Chen,J. and
            Yu,X.
  TITLE     Histone ubiquitination associates with BRCA1-dependent DNA damage
            response
  JOURNAL   Mol Cell Biol 29 (3), 849-860 (2009)
   PUBMED   19015238
REFERENCE   8  (bases 1 to 2096)
  AUTHORS   Huen,M.S., Huang,J., Yuan,J., Yamamoto,M., Akira,S., Ashley,C.,
            Xiao,W. and Chen,J.
  TITLE     Noncanonical E2 variant-independent function of UBC13 in promoting
            checkpoint protein assembly
  JOURNAL   Mol Cell Biol 28 (19), 6104-6112 (2008)
   PUBMED   18678647
REFERENCE   9  (bases 1 to 2096)
  AUTHORS   Minter-Dykhouse,K., Ward,I., Huen,M.S., Chen,J. and Lou,Z.
  TITLE     Distinct versus overlapping functions of MDC1 and 53BP1 in DNA
            damage response and tumorigenesis
  JOURNAL   J Cell Biol 181 (5), 727-735 (2008)
   PUBMED   18504301
REFERENCE   10 (bases 1 to 2096)
  AUTHORS   Pfeuffer,T., Goebel,W., Laubinger,J., Bachmann,M. and Kuhn,M.
  TITLE     LaXp180, a mammalian ActA-binding protein, identified with the
            yeast two-hybrid system, co-localizes with intracellular Listeria
            monocytogenes
  JOURNAL   Cell Microbiol 2 (2), 101-114 (2000)
   PUBMED   11207567
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163629.5, AK147168.1 and CO043810.1.
            
            On Jun 18, 2015 this sequence version replaced NM_021419.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021778.1, AK147168.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC163629.5         15643-15663         c
            22-2064             AK147168.1         2-2044
            2065-2096           CO043810.1         1-32                c
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 15.45 cM"
     gene            1..2096
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /note="ring finger protein 8"
                     /db_xref="GeneID:58230"
                     /db_xref="MGI:MGI:1929069"
     exon            1..290
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..1646
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /EC_number="2.3.2.27"
                     /note="actA-interacting protein 37; ActA binding protein
                     2; RING-type E3 ubiquitin transferase RNF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF8"
                     /protein_id="NP_067394.1"
                     /db_xref="CCDS:CCDS37538.1"
                     /db_xref="GeneID:58230"
                     /db_xref="MGI:MGI:1929069"
                     /translation="MGEPDPLVSGQLAARRSWCLRRLGMDCEWLQLEAGTEVTIGRGL
                     SVTYQLISKVCPLMISRSHCVLKQNPEGQWTIMDNKSLNGVWLNRERLAPLQGYCIRK
                     GDHIQLGVPLESRETAEYEYEVIEEDWESLAPCLAPKNDQRMEKHKGSRTKRKFSSPG
                     LENLPAEGSSDLRCPLANVASKPIEPEKLHGKGDASSQSLGCLCPGLTSLKASERAAG
                     PHACSALPKVLELSCPKKQKACRPSASQNSLELFKVTMSRMLKLKTQMQEKQIAVLNV
                     KRQTRKGSSKKIVRMEKELRNLQSQLYAEQAQQQARVEQLEKTFQEEAHYLQGLEKEQ
                     GECDLKQQLVQALQEHQALMEELNCSKKDFEKIIQAKNKELEQTKEEKDKVQAQKEEV
                     LSHMNDLLENELQCIICSEYFIEAVTLNCAHSFCSFCINEWMKRKVECPICRKDIESR
                     TNSLVLDNCISKMVDNLSSDVKERRSVLIRERRAKRLS"
     exon            291..419
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            420..1163
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1226
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1316
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1424
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1629
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..2078
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1870..1875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /note="hexamer: ATTAAA"
     polyA_site      1899
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
     regulatory      2044..2049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /note="hexamer: AATAAA"
     polyA_site      2065
                     /gene="Rnf8"
                     /gene_synonym="3830404E21Rik; AIP37; laXp180"
                     /note="major polyA site"
ORIGIN      
        1 cgcgcactgc ccgccttccc aggcctgggg cgggcccgcg ctctcaacgc ggtcgatccc
       61 cgggcgattg cttctgtctt gtgtgtagat taagttgcgc gagaggaacc tcggaggcgg
      121 ccggagctca ggctagaggt tggctggttg ctgaacgctg ccccagtgcc ggtcgggcca
      181 tgggcgagcc cgaccccttg gtctcggggc agctcgcggc ccgccggagc tggtgcttgc
      241 ggcggctggg gatggactgc gagtggctgc agctggaggc tgggactgag gtgaccatag
      301 gacggggact tagtgtcaca taccagctga tatcaaaggt ttgccctctg atgatttccc
      361 gaagccactg tgttctgaag cagaatcctg agggacagtg gaccattatg gacaataaga
      421 gtctgaatgg tgtttggctg aacagagaac gtctggcacc attacagggt tattgcatcc
      481 gtaagggaga ccatatccag ctcggtgtgc cgctggaaag cagggagacg gcggagtatg
      541 aatacgaagt cattgaagaa gactgggaga gtctggctcc ctgccttgcc ccgaagaatg
      601 accagagaat ggaaaaacat aaaggctcga gaactaagag gaaattcagt tcgcctgggt
      661 tagagaatct tccagccgag ggctcctcag atctcagatg cccactggct aatgtcgcca
      721 gtaaacccat tgagccagag aagttgcatg gcaaaggtga cgcatcgagt cagtccttgg
      781 gatgcttgtg tcctgggctg acttctctga aggcgagtga gagggccgcc gggcctcatg
      841 cttgctctgc tcttccaaag gtcctggaac tcagttgtcc taagaagcag aaggcctgca
      901 gaccatcagc atctcagaac agcttagaac tgtttaaggt gaccatgtcc aggatgctga
      961 agctgaaaac acagatgcag gagaagcaga tagctgtcct aaacgtcaag agacagactc
     1021 ggaaggggag ctcaaagaag atcgtgagga tggagaagga gctgcgaaac ttacagtctc
     1081 agctgtatgc agagcaggct cagcagcaag ccagagtgga gcagctggag aagactttcc
     1141 aggaggaggc acattacctc cagggtttgg agaaagagca aggagagtgc gacctgaagc
     1201 aacagctggt gcaggccctg caggagcatc aggctctaat ggaagaacta aattgcagca
     1261 agaaggactt tgagaaaatc attcaagcca agaacaaaga attggagcag accaaggaag
     1321 aaaaggacaa ggtgcaagcg cagaaggagg aggttctcag ccacatgaac gacttgctgg
     1381 agaacgagct ccagtgcatt atctgctcgg agtactttat tgaggctgtc accctgaact
     1441 gtgcccacag tttctgctcc ttctgtatca atgaatggat gaaacggaaa gtagagtgtc
     1501 ccatttgtcg aaaggacatt gagtccagaa ccaactccct ggttctggac aactgcatta
     1561 gtaagatggt ggataaccta agctcagacg tgaaggaaag aagaagtgtc cttattaggg
     1621 aacggagagc aaagagactg tcatgaaggt cacgctgcaa ggacatgtga gagactctag
     1681 ggcgatggcg gcttggcttg ttctagagga ttccctggat tccctgaggc tgctctggac
     1741 gtcttctgga agccatgcgc tgcagagact gagttaggaa gtctgtgcca ctggccttcc
     1801 gtcatgcagg ccctgctgct accacccccc aacgtgtgct gggaccctgc tcggactgcc
     1861 tgactgtgca ttaaatgagt tgagttctgt gtttcttaat ttgttgattg tttttgatac
     1921 ctcatgcacc ctcttgacat cattgtggac agctgggtgt gagtcctgtg gctgtgtgga
     1981 aggtggcctg cattttgaga gcctggttta cctttttatg aaaacatgat gtcatttctt
     2041 gggaataaaa tgtataaaac tctcagttgc tcagctggaa aaaaaaaaaa aaaaaa
//
