LOCUS       NM_001289757            1606 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ring finger protein 32 (Rnf32), transcript variant 1,
            mRNA.
ACCESSION   NM_001289757 XM_006535773
VERSION     NM_001289757.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Sagai,T., Amano,T., Maeno,A., Ajima,R. and Shiroishi,T.
  TITLE     SHH signaling mediated by a prechordal and brain enhancer controls
            forebrain organization
  JOURNAL   Proc Natl Acad Sci U S A 116 (47), 23636-23642 (2019)
   PUBMED   31685615
REFERENCE   3  (bases 1 to 1606)
  AUTHORS   Symmons,O., Pan,L., Remeseiro,S., Aktas,T., Klein,F., Huber,W. and
            Spitz,F.
  TITLE     The Shh Topological Domain Facilitates the Action of Remote
            Enhancers by Reducing the Effects of Genomic Distances
  JOURNAL   Dev Cell 39 (5), 529-543 (2016)
   PUBMED   27867070
REFERENCE   4  (bases 1 to 1606)
  AUTHORS   Anderson,E., Devenney,P.S., Hill,R.E. and Lettice,L.A.
  TITLE     Mapping the Shh long-range regulatory domain
  JOURNAL   Development 141 (20), 3934-3943 (2014)
   PUBMED   25252942
REFERENCE   5  (bases 1 to 1606)
  AUTHORS   Wiese,C.B., Ireland,S., Fleming,N.L., Yu,J., Valerius,M.T.,
            Georgas,K., Chiu,H.S., Brennan,J., Armstrong,J., Little,M.H.,
            McMahon,A.P. and Southard-Smith,E.M.
  TITLE     A genome-wide screen to identify transcription factors expressed in
            pelvic Ganglia of the lower urinary tract
  JOURNAL   Front Neurosci 6, 130 (2012)
   PUBMED   22988430
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1606)
  AUTHORS   Amano,T., Sagai,T., Tanabe,H., Mizushina,Y., Nakazawa,H. and
            Shiroishi,T.
  TITLE     Chromosomal dynamics at the Shh locus: limb bud-specific
            differential regulation of competence and active transcription
  JOURNAL   Dev Cell 16 (1), 47-57 (2009)
   PUBMED   19097946
REFERENCE   7  (bases 1 to 1606)
  AUTHORS   Niedermaier,M., Schwabe,G.C., Fees,S., Helmrich,A., Brieske,N.,
            Seemann,P., Hecht,J., Seitz,V., Stricker,S., Leschik,G.,
            Schrock,E., Selby,P.B. and Mundlos,S.
  TITLE     An inversion involving the mouse Shh locus results in brachydactyly
            through dysregulation of Shh expression
  JOURNAL   J Clin Invest 115 (4), 900-909 (2005)
   PUBMED   15841179
REFERENCE   8  (bases 1 to 1606)
  AUTHORS   van Baren,M.J., van der Linde,H.C., Breedveld,G.J., Baarends,W.M.,
            Rizzu,P., de Graaff,E., Oostra,B.A. and Heutink,P.
  TITLE     A double RING-H2 domain in RNF32, a gene expressed during sperm
            formation
  JOURNAL   Biochem Biophys Res Commun 292 (1), 58-65 (2002)
   PUBMED   11890671
REFERENCE   9  (bases 1 to 1606)
  AUTHORS   Clark,R.M., Marker,P.C. and Kingsley,D.M.
  TITLE     A novel candidate gene for mouse and human preaxial polydactyly
            with altered expression in limbs of Hemimelic extra-toes mutant
            mice
  JOURNAL   Genomics 67 (1), 19-27 (2000)
   PUBMED   10945466
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK012288.1, BY293373.1, BC089493.1 and AV284199.1.
            
            On Jan 29, 2014 this sequence version replaced XM_006535773.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006379.1, BC050964.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AK012288.1         2-3
            3-22                BY293373.1         2-21
            23-85               AK012288.1         23-85
            86-1523             BC089493.1         1-1438
            1524-1563           AK012288.1         1256-1295
            1564-1606           AV284199.1         191-233
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 14.78 cM"
     gene            1..1606
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /note="ring finger protein 32"
                     /db_xref="GeneID:56874"
                     /db_xref="MGI:MGI:1861747"
     exon            1..120
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            121..186
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            187..281
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..1373
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /note="limb region protein 2"
                     /codon_start=1
                     /product="RING finger protein 32"
                     /protein_id="NP_001276686.1"
                     /db_xref="CCDS:CCDS39041.1"
                     /db_xref="GeneID:56874"
                     /db_xref="MGI:MGI:1861747"
                     /translation="MLRNKDRSSNKGNLAVTAVALQDHLLHDLQLGHLSVADPCKIKA
                     RKKEKKSKSLKRDATAIIDTGLRKSTEGPNMEDPEKEYVLDPTPPPLTLAQKLGLLPP
                     PPLPLSSDEWERVKQRSLLQGDSIQPCPICKEEFELHPQVLLSCSHVFHRACLQAFEK
                     FTNKKTCPLCRKNQYQTRVIHDGARLFRVKCATRIQAYWRGYIVRKWYRNLRKIIPPS
                     DAKLRRKFFEEKFTEISHRILCSYNTNIDELFSEIDVCLAVNRSILQQLDERCGQIIT
                     KEDWEKIQAQAAHHEIYECSICLTPLSFHGDGRQAAIGTSSQRPRETVLLSCAHLFHN
                     ACLLALEEFSLGDNAPFHVCPLCRSCYQKKIVEC"
     exon            282..546
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            547..689
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            690..722
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..847
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            848..956
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1124
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1606
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1540..1545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /note="hexamer: ATTAAA"
     polyA_site      1567
                     /gene="Rnf32"
                     /gene_synonym="1700009J01Rik; 2700025B22Rik;
                     4930542N22Rik; Lmbr2"
                     /note="major polyA site"
ORIGIN      
        1 ctgagagcca ggcggagccg gccggcgggc gtgcgcacct agaagcgcag aggccgctgg
       61 ttgccgtggc aacgaggagc cgttcagaaa aggtgcagct gagggacagg aaaggccgtt
      121 ttggactaca tctgacacgt ttcgttatcc tttcaattgt cagtttgcta atactatggg
      181 actatgcaga agaatggaag ttatcgtaac gtgatgttag agatcgttaa agccacacca
      241 cagtgtttcc tgattcctga tacagcatgc taagaaataa ggatcgttca tctaacaaag
      301 gtaacttagc agtcactgca gttgccttac aggaccacct attacatgat cttcaacttg
      361 gacatctctc ggtagcagat ccttgtaaga taaaagcacg aaagaaagag aagaagtcaa
      421 aatctctcaa aagagatgca acagccatca tagatactgg gcttagaaaa tctacagagg
      481 gtcctaacat ggaagatcca gagaaggaat atgttctaga ccccacacca ccaccattaa
      541 ctttagccca gaaactaggc ctcttgccac ctcccccgtt gccactgtca tcagatgagt
      601 gggagagagt gaagcagaga tcccttctac aaggggactc cattcagcca tgcccaatat
      661 gtaaagaaga atttgagctc catccccagg tgctgctttc ctgctcacat gtattccata
      721 gggcatgcct tcaggctttt gaaaagttca caaataaaaa aacctgtccc ctctgtagaa
      781 agaaccaata tcaaacaaga gtgatacatg atggggctcg cctgttcaga gtaaagtgtg
      841 ccaccaggat ccaagcctat tggcgagggt acatcgttag aaagtggtac agaaacctga
      901 ggaaaataat cccgccttcg gatgccaaat taaggagaaa gttctttgag gaaaagttca
      961 cagagatcag ccaccgaatc ctgtgctcat acaacaccaa catagatgaa ctcttctcag
     1021 aaattgatgt ctgcttggcg gtaaaccgta gcattcttca gcagctagat gaaaggtgtg
     1081 gccagataat caccaaggag gactgggaga aaatccaagc acaggctgct caccatgaga
     1141 tctatgaatg ttccatctgc ctgactccac tctcctttca tggcgatggc cggcaagcag
     1201 ccataggaac cagcagccag cgtcccagag agacagtcct cctgtcctgt gcacatctgt
     1261 tccacaatgc ctgcctcctg gctttggagg agttttcttt aggagacaat gcccctttcc
     1321 atgtctgtcc tctctgtcgc tcctgctacc aaaagaaaat tgttgaatgc tgaattcatg
     1381 tccaggaagt taaatgtctg actagaacaa gaaaggggta aaggctatcc tgcttttgga
     1441 tgagctgcat gggtttgggg tagaggacta cattgctgac tgttctttgt gattatatag
     1501 aggaaatata agttcctatt taactgtggt ctttattgca ttaaaaacag catgttcgtt
     1561 tgttgaattt agttacttaa aataaatgag aagtagtgtt aattaa
//
