LOCUS       NM_080561               4521 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ring finger protein 216 (Rnf216), transcript variant
            A, mRNA.
ACCESSION   NM_080561 XM_144627
VERSION     NM_080561.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Kong,C., Guo,Z., Liu,F., Tang,N., Wang,M., Yang,D., Li,C., Yang,Z.,
            Ma,Y., Wang,P. and Tang,Q.
  TITLE     Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9
            impairs mitochondrial bioenergetics and exacerbates diabetic
            cardiomyopathy
  JOURNAL   J Adv Res 61, 65-81 (2024)
   PUBMED   37625569
  REMARK    GeneRIF: Triad3A-Mediated K48-Linked ubiquitination and degradation
            of TLR9 impairs mitochondrial bioenergetics and exacerbates
            diabetic cardiomyopathy.
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4521)
  AUTHORS   George,A.J., Wei,W., Pyaram,D.N., Gomez,M., Shree,N., Kadirvelu,J.,
            Lail,H., Wanders,D., Murphy,A.Z. and Mabb,A.M.
  TITLE     Gordon Holmes Syndrome Model Mice Exhibit Alterations in Microglia,
            Age, and Sex-Specific Disruptions in Cognitive and Proprioceptive
            Function
  JOURNAL   eNeuro 11 (1) (2024)
   PUBMED   38164552
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4521)
  AUTHORS   Yang,H., Zhu,Y., Li,X., Jiang,Z. and Dai,W.
  TITLE     RNF216 affects the stability of STAU2 in the hypothalamus
  JOURNAL   Dev Growth Differ 65 (7), 408-417 (2023)
   PUBMED   37439148
  REMARK    GeneRIF: RNF216 affects the stability of STAU2 in the hypothalamus.
REFERENCE   5  (bases 1 to 4521)
  AUTHORS   George,A.J., Dong,B., Lail,H., Gomez,M., Hoffiz,Y.C., Ware,C.B.,
            Fang,N., Murphy,A.Z., Hrabovszky,E., Wanders,D. and Mabb,A.M.
  TITLE     The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the
            hypothalamic-pituitary-gonadal axis
  JOURNAL   iScience 25 (6), 104386 (2022)
   PUBMED   35620441
  REMARK    Erratum:[iScience. 2022 Sep 14;25(10):105108. doi:
            10.1016/j.isci.2022.105108.. PMID: 36147949]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4521)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   7  (bases 1 to 4521)
  AUTHORS   Mabb,A.M., Je,H.S., Wall,M.J., Robinson,C.G., Larsen,R.S.,
            Qiang,Y., Correa,S.A. and Ehlers,M.D.
  TITLE     Triad3A regulates synaptic strength by ubiquitination of Arc
  JOURNAL   Neuron 82 (6), 1299-1316 (2014)
   PUBMED   24945773
REFERENCE   8  (bases 1 to 4521)
  AUTHORS   Xu,C., Feng,K., Zhao,X., Huang,S., Cheng,Y., Qian,L., Wang,Y.,
            Sun,H., Jin,M., Chuang,T.H. and Zhang,Y.
  TITLE     Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1
            ubiquitination
  JOURNAL   Autophagy 10 (12), 2239-2250 (2014)
   PUBMED   25484083
  REMARK    GeneRIF: identifies a novel negative regulator of autophagy and
            suggests that RNF216 may be a target for treatment of inflammatory
            diseases
REFERENCE   9  (bases 1 to 4521)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4521)
  AUTHORS   Martinez-Noel,G., Niedenthal,R., Tamura,T. and Harbers,K.
  TITLE     A family of structurally related RING finger proteins interacts
            specifically with the ubiquitin-conjugating enzyme UbcM4
  JOURNAL   FEBS Lett 454 (3), 257-261 (1999)
   PUBMED   10431818
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB854046.1 and BC065066.1.
            
            On Jul 20, 2010 this sequence version replaced NM_080561.3.
            
            Transcript Variant: This variant (A) uses an alternate in-frame
            splice junction compared to variant B. The resulting isoform (A)
            has the same N- and C-termini but is shorter compared to isoform B.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065066.1, AK154368.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BB854046.1         2-27
            27-4521             BC065066.1         1-4495
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 81.86 cM"
     gene            1..4521
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /note="ring finger protein 216"
                     /db_xref="GeneID:108086"
                     /db_xref="MGI:MGI:1344349"
     exon            1..122
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            123..249
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2744
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /EC_number="2.3.2.27"
                     /note="isoform A is encoded by transcript variant A; E3
                     ubiquitin-protein ligase RNF216; E3 ubiquitin ligase;
                     ubcM4-interacting protein 83; triad domain-containing
                     protein 3; ubiquitin-conjugating enzyme 7-interacting
                     protein 1; RING-type E3 ubiquitin transferase RNF216"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF216 isoform A"
                     /protein_id="NP_542128.2"
                     /db_xref="CCDS:CCDS80455.1"
                     /db_xref="GeneID:108086"
                     /db_xref="MGI:MGI:1344349"
                     /translation="MAEGNNKEEVIHLNNFPCHRGKEWMAVREGPITISDSSDEEGIP
                     MLVTPATEQQEDDLDDDVILTEDDSEDEYGGFLDLESGKKEGEAKPGPSSKQTADDIV
                     NPRLEQKVIILGENGLLFPESEPLEVQNQSSEDSETELLSNPGEPAASVDDQLIGEEY
                     WLDHPYFQAPNPQPQERTNQVVPQERHSESEMGPMFFRHDFPEPAFPRPEPQQEGIPG
                     PASPQPAHPLGELEDQQLAIDEDPGPAFPLSGPQEANLANMWEQEAAEVDQDLIPLLV
                     KETEARFPDVASGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRLIIHPSSSLLAS
                     QDDAKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKMLSSQDIKWALHELKGHYAIT
                     RKAFSDAIKKWQELSPETSGKRKKRKEMNQYSFIDFKFEQGNIKIEKRMFFLENKRRH
                     CRYYDHQALLPAVKQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYG
                     EFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPTSELEKVLP
                     QTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKC
                     QGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCR
                     CGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAE
                     EEQKRKNGENTFKRIGPPLEKPAEKVQRVEALPRPVPQNLHPQMPPYAFVHPPFPLPP
                     VRPVFNNFPINMGPVPAPYVPPLPNVRVNYDFGHMHVPLEHNLPMHFGPQPRHRF"
     exon            250..380
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            381..1019
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1096
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1199
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1364
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1479
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1619
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1670
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1808
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1957
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2036
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2134
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2357
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2427
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..4500
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4480..4485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /note="hexamer: AATAAA"
     polyA_site      4500
                     /gene="Rnf216"
                     /gene_synonym="2810055G22Rik; F830018F18Rik; TRIAD3;
                     Ubce7ip1; UIP83"
                     /note="major polyA site"
ORIGIN      
        1 aggcgcgagg tggacgcggt ggcgaggccg gaaggaacgc agtagcggcc tcggtggcgg
       61 cggcggcggt cgcagcggcg gcggtcgcag cgggggagct tttccagcgg ctgcttcttc
      121 agaacagatg atcaccatgt ttggttacta gcaatcacac atgtttgtat ctgaatttga
      181 aaatggcaga gggaaacaac aaagaggagg tcattcactt gaacaacttt ccctgccacc
      241 ggggaaagga atggatggct gtcagagagg ggcccatcac catatctgac tcctctgatg
      301 aggaagggat tccaatgctg gttaccccag ctacagagca acaggaagac gacctggatg
      361 atgatgtcat cctgacagaa gatgattctg aggatgaata tggtggattt ctggatcttg
      421 agagtggcaa aaaagaggga gaagccaaac ctggaccaag cagtaagcag acagcagacg
      481 atattgtcaa ccccagattg gagcagaaag tcattatatt gggagaaaat ggtcttcttt
      541 tcccagaaag tgagcctttg gaagttcaga accaatcatc cgaagactca gagacagagc
      601 tcttatcaaa tcctggagag ccagctgcct cagtagatga ccagctaatt ggagaagagt
      661 actggcttga ccatccatac ttccaggctc cgaatccaca gcctcaggaa agaacaaacc
      721 aggttgtgcc ccaagagcga cattctgaat cagaaatggg cccaatgttt ttccgccatg
      781 atttcccaga gccagctttt ccaaggccag aaccccagca ggaggggatt ccaggccctg
      841 cttctccaca gccagcccat cctctaggag agcttgaaga ccagcagtta gcaattgatg
      901 aagaccctgg gccagccttc cccctgtcag gacctcagga ggccaacttg gcaaacatgt
      961 gggaacaaga agctgctgag gtagatcaag acctcattcc actgttagtg aaagaaacgg
     1021 aagcaagatt tccagatgta gcaagtggat atgttgaaga aataattcat ttgaaaaatt
     1081 actatgattt gaatgtactt tgtaattttc ttctggaaaa cccagattat ccaaagagag
     1141 aagaccggct tattatccac cccagcagca gcctgcttgc cagccaggat gacgcgaagt
     1201 tgcctaaaat agactttttt gactattcca aattgactcc acttgaccag cgctgcttca
     1261 tccaagctgc tgacctcctg atggctgatt tcaagatgct tagcagccag gacatcaagt
     1321 gggcccttca tgagctcaaa ggacactatg caatcacccg aaaggccttt tccgatgcca
     1381 ttaagaaatg gcaggagttg tcacctgaaa ccagtggaaa acggaaaaag aggaaagaaa
     1441 tgaatcagta ttctttcata gatttcaagt ttgaacaagg aaacataaaa atagaaaaga
     1501 ggatgttctt tctggaaaac aagcgtcgac actgtaggta ctatgaccat caggccctcc
     1561 ttccagctgt gaaacaggag caggaattct atgagcagaa aattaaagag atggcagagc
     1621 atgaagactt cttgctcgcc ctgcagatga atgaagaaca gtatcaaaag gatggccagc
     1681 tgattgaatg ccgctgttgc tatggggagt ttccatttga ggagctgaca caatgcgccg
     1741 atgctcactt gttctgcaaa gaatgtctca tcagatatgc ccaggaggca gtgtttggat
     1801 ctggaaagtc agaactcagc tgcatggaag gcagctgcac gtgctccttc ccaaccagtg
     1861 agctggagaa ggtgctcccc cagaccattc tgtacaaata ctatgaacgg aaagctgagg
     1921 aagaagtcgc tgcagcctat gctgatgagc ttgtccggtg cccctcctgc agcttccctg
     1981 ctctgttgga cagcgatgtg aagaggttca gctgccccaa cccacgctgc cgaaaggaga
     2041 cctgtaggaa gtgtcaggga ctctggaaag aacacaacgg cctcacctgt gaagagctgg
     2101 cagaaaagga tgacatcaag taccggacat ccattgaaga aaaaatgact gctgctcgga
     2161 ttagaaaatg tcacaagtgt ggaactggcc tcatcaagtc tgaaggctgc aaccgcatgt
     2221 cttgccgctg tggtgcccag atgtgctacc tctgtcgagt ttctatcaat ggctatgacc
     2281 atttctgcca gcatcctcgc tctccaggag ccccttgcca agaatgttcc aggtgctccc
     2341 tgtggaccga ccccactgaa gatgatgaaa agctgattga ggaaatccaa aaggaggccg
     2401 aggaggaaca gaagagaaag aatggagaga acaccttcaa acgcatcggg cccccactgg
     2461 agaagccagc tgagaaggtg cagcgtgtgg aagccctgcc gcggcctgtc ccacagaacc
     2521 tgcatcctca gatgccgccc tatgcctttg ttcacccacc cttccccctg ccacctgtgc
     2581 ggcccgtgtt caacaacttc cccatcaaca tgggtcctgt gcccgctccc tatgtccccc
     2641 ctctgcccaa cgtgcgtgtc aactatgact ttggccacat gcacgtgccc ctggagcaca
     2701 acctgcccat gcactttggc ccccaaccac ggcatcgctt ctgacaccca aagccctgtc
     2761 agccgtgccg agtctgcagg agggcccagt ctcatcttct gagtaggggt gaaggcctcc
     2821 attccctctc gaaagtggac gcgtgtcctc ctgctcttac ctttgcaagg tccatgctcc
     2881 ttcaggtctg atgccctctg ggtgctgatt gtcactgggc caattatagg gcagctccct
     2941 agtctgccat cttagcagcc aatccagtgg ccctgaccat gaagcaaggc ctctaatcgt
     3001 ttgccatact tcctccccag cagcccaatg aaagcccagg gggaaatggc ctaccatccc
     3061 taagccaggg ctctctcctt gttgcccaag gcccacttag gtccaagagc ctccgagaat
     3121 ctgtggcgcg agttttgttc tgttgttatc taaagaggtt gctcctattc atgtagtttg
     3181 agctgaagtt ttttttgtgg tctactcatg cgtgtcccta tccgtggaaa gtcaggttga
     3241 aagtatttcc ccagggctgg caactgctga gagccagaag tcctgacact tgccatcaaa
     3301 caaaagggcc ctttaggaag ctgtcattta ttcccaggtg ggccctgttc tggggtgagc
     3361 agcttctttc tcctaaggtg ttgggggttc cctggcagtg cagatgacct catggagctg
     3421 ctacagtcat cacagcaacc ctgctgcctg ctccccgtcc ctcactagtt agcagtgggt
     3481 gcacacagga gagctcccag ctcctgtgtg atgagagtcc aggcctgcac agagcctttg
     3541 ccatgttccc agtgcctgtg tcccctcctg tccagaccag gcttttgtcc tccagcgttt
     3601 gctgagcagg accggcagca ggtttctcag ttgtactggc tgtccttgtc ctccccttgc
     3661 tgtgggggtg tcccctgttc agagtgtaca gacttagaag ggggaaagtc cctgggaaag
     3721 ggaaggctcc ctgagctgcc cattatcact ggagtgtggg tggggcacac agtggctcag
     3781 gagccctgcc atggtcacct gaactgtgat gttagtctga atgacagtgc cttggaaaga
     3841 tgggccacat gctgcatgac aggatcctcc aagtacctta actgttagag tatccgtgtt
     3901 ctctgcactt gtccagaagc cccacgcatg ggtgggtggc tgggtgggta ctccaagcac
     3961 tgcccctcca cggcctcgtg agccgctgga ctgggcaccc agttctaggt tgactcagta
     4021 cccgtctcac atccttccac caggagacca gatgcaaaca actggcagtt tctggggtga
     4081 ggccagggtc gtgtcagtct gtgggtgcag ggaggctgct cctgcctgtc tccatccagg
     4141 agtgaccctc ccaacacctc tgtgccagca gggatcccca aggcagctac caccttaaca
     4201 tctaggaccc ggactgcttc ctggtgtgag aaactgccta gccactccca gtgtatttgc
     4261 cttaaaactg gagagaatct agtatttgca cttagcaaaa gatttcaaag ttaagagaaa
     4321 agtgcatgat ttcactttgt acaaaagaaa aaaagaaaaa aaaatgaagt agatggttaa
     4381 aagtttttct ttaccttttg gtagtaaaaa tagaggaacc aagtctgttt aaactactgt
     4441 gtaaaaagac tgtatcatat gtaacttgac ttttgtgtaa ataaatgttt gtgaacgctt
     4501 aaaaaaaaaa aaaaaaaaaa a
//
