LOCUS       NM_182999               4159 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus ring finger protein 20 (Rnf20), transcript variant 2,
            mRNA.
ACCESSION   NM_182999
VERSION     NM_182999.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Tetik-Elsherbiny,N., Elsherbiny,A., Setya,A., Gahn,J., Tang,Y.,
            Gupta,P., Dou,Y., Serke,H., Wieland,T., Dubrac,A., Heineke,J.,
            Potente,M., Cordero,J., Ola,R. and Dobreva,G.
  TITLE     RNF20-mediated transcriptional pausing and VEGFA splicing
            orchestrate vessel growth
  JOURNAL   Nat Cardiovasc Res 3 (10), 1199-1216 (2024)
   PUBMED   39322771
  REMARK    GeneRIF: RNF20-mediated transcriptional pausing and VEGFA splicing
            orchestrate vessel growth.
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4159)
  AUTHORS   Jeon,Y.G., Nahmgoong,H., Oh,J., Lee,D., Kim,D.W., Kim,J.E.,
            Kim,Y.Y., Ji,Y., Han,J.S., Kim,S.M., Sohn,J.H., Lee,W.T., Kim,S.W.,
            Park,J., Huh,J.Y., Jo,K., Cho,J.Y., Park,J. and Kim,J.B.
  TITLE     Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis
            with brown and beige fat-specific substrates
  JOURNAL   Nat Commun 15 (1), 940 (2024)
   PUBMED   38296968
  REMARK    GeneRIF: Ubiquitin ligase RNF20 coordinates sequential adipose
            thermogenesis with brown and beige fat-specific substrates.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4159)
  AUTHORS   Kim,Y.J., Oh,S.H., Lim,J.H., Cho,J.H., Jung,H.Y., Kim,C.D.,
            Park,S.H., Kwon,T.H. and Kim,Y.L.
  TITLE     Impact of Ring Finger Protein 20 and Its Downstream Regulation on
            Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a
            High-Fat Diet
  JOURNAL   Nutrients 15 (23), 4959 (2023)
   PUBMED   38068817
  REMARK    GeneRIF: Impact of Ring Finger Protein 20 and Its Downstream
            Regulation on Renal Tubular Injury in a Unilateral Nephrectomy
            Mouse Model Fed a High-Fat Diet.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4159)
  AUTHORS   Lin,C.Y., Chang,Y.M., Tseng,H.Y., Shih,Y.L., Yeh,H.H., Liao,Y.R.,
            Tang,H.H., Hsu,C.L., Chen,C.C., Yan,Y.T. and Kao,C.F.
  TITLE     Epigenetic regulator RNF20 underlies temporal hierarchy of gene
            expression to regulate postnatal cardiomyocyte polarization
  JOURNAL   Cell Rep 42 (11), 113416 (2023)
   PUBMED   37967007
  REMARK    GeneRIF: Epigenetic regulator RNF20 underlies temporal hierarchy of
            gene expression to regulate postnatal cardiomyocyte polarization.
REFERENCE   6  (bases 1 to 4159)
  AUTHORS   Chernikova,S.B., Dorth,J.A., Razorenova,O.V., Game,J.C. and
            Brown,J.M.
  TITLE     Deficiency in Bre1 impairs homologous recombination repair and cell
            cycle checkpoint response to radiation damage in mammalian cells
  JOURNAL   Radiat Res 174 (5), 558-565 (2010)
   PUBMED   20738173
  REMARK    GeneRIF: the observed defects in the radiation response of
            Bre1a/b-deficient cells
REFERENCE   7  (bases 1 to 4159)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 4159)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 4159)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4159)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ052397.1, AK053783.1, AK139883.1, BQ442966.1, CN531555.1,
            DV660388.1 and BF730643.1.
            
            On Jul 14, 2009 this sequence version replaced NM_182999.1.
            
            Transcript Variant: This variant (2) lacks a segment in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052482.1, SRR1660813.93465.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                CJ052397.1         5-17
            14-1163             AK053783.1         2-1151
            1164-2175           AK139883.1         692-1703
            2176-2805           BQ442966.1         91-720
            2806-3276           CN531555.1         205-675
            3277-3819           DV660388.1         138-680
            3820-4159           BF730643.1         1-340               c
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 26.69 cM"
     gene            1..4159
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /note="ring finger protein 20"
                     /db_xref="GeneID:109331"
                     /db_xref="MGI:MGI:1925927"
     exon            1..152
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            153..300
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            301..457
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..3250
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase BRE1A; BRE1-A; RING-type E3
                     ubiquitin transferase BRE1A"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase BRE1A isoform 1"
                     /protein_id="NP_892044.1"
                     /db_xref="CCDS:CCDS18178.1"
                     /db_xref="GeneID:109331"
                     /db_xref="MGI:MGI:1925927"
                     /translation="MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTE
                     ELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDE
                     NIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGDGQEPAFSFLA
                     TLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIVEEAV
                     QELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQW
                     DIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEE
                     NKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLY
                     NESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRK
                     EYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDL
                     NKTRLRSGSALLQSQSSTEDPKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEE
                     RERERREKEREREREREKEKEREREKQKLKESEKERDSVKDKEKGKHDDGRKKEAEII
                     KQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDL
                     EDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDM
                     QEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQ
                     VVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKL
                     HDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDY
                     KARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI
                     G"
     exon            458..625
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            626..773
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            774..956
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1075
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1222
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1300
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1420
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1600
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1736
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1858
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..2223
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2341
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2491
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2704
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2830
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2831..2971
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3073
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..4159
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4139..4144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /note="hexamer: ATTAAA"
     polyA_site      4159
                     /gene="Rnf20"
                     /gene_synonym="4833430L21Rik; mKIAA4116"
                     /note="major polyA site"
ORIGIN      
        1 ttttccctac ttcctctccg tgcctccgcc tccgtggagc tcagtccctc tcgcagcgga
       61 tcccaagtga atcccttgga gtccctctcg ggccttcgtg aagaagcgct gggagccgcg
      121 cgctccctgc ggcgggagtt gccagagccc aggcgaggtc caacccagat tgctgcttgc
      181 cattgacgcc tcgtcccgga gtttggatcg gaagccggct ggatgtagac ccctgatggc
      241 cgcggtgctc gccatctagc atccattcca cctccttgtg agtttcggaa ccagagtcag
      301 aaaaatataa ctgtcatctt ataccaagat gtcaggaatt ggaaataaaa gagcagctgg
      361 agagccgggc acatctatgc ctccagagaa gaagacagct gttgaagatt cagggaccac
      421 cgtggaaaca attaagttag gaggtgtctc ttcaacggag gaattagaca ttcgaacact
      481 gcaatccaag aatcgtaagc tagcagaaat gctagatcag cggcaggcca ttgaagatga
      541 gcttcgggaa cacattgaaa agctggagcg acgccaggcc actgatgatg cctcactgtt
      601 gattgtgaac cggtactgga gccagtttga tgaaaacatc cgcatcatcc ttaaacgtta
      661 tgatctggac cagggtttgg gggacctact cacagaaagg aaagccttgg ttgtgccaga
      721 accagaacct gactctgata gcaatcagga acgcaaagat gaccgtgaga gaggggatgg
      781 gcaagagcca gctttctctt tccttgctac tttggctagc agttccagtg aagagatgga
      841 atctcagctt caggagcgtg tagagtcctc ccgccgtgct gtgtcccaga ttgtgactgt
      901 ctatgataaa ttgcaagaaa aagtggatct cttatcccgg aaactgaaca gtggagataa
      961 cctgatagtg gaggaagcag tgcaggagtt gaactccttc cttgcacaag agaatgtgag
     1021 gcttcaggag ctgacagacc tccttcagga gaagcatcac accatgtctc aggagttctg
     1081 taagttgcag ggtaaagtag agacagctga gtcacgagtg tccgtcttgg agtccatgat
     1141 tgatgacctg cagtgggata ttgacaaaat tcgaaaaaga gaacagcgtc tcaaccggca
     1201 tttggctgaa gtcctagagc gggtgaattc aaaaggctat aaagtatatg gagcggggag
     1261 cagtctctat ggaggcacaa tcaccatcaa tgcccggaag tttgaggaaa tgaatgcaga
     1321 gcttgaggag aacaaagagt tggctcagaa ccgacattgt gagctggaga agcttcgtca
     1381 agactttgaa gaggtcacta cacagaatga aaagttgaag gtggaattgc ggagtgcagt
     1441 ggaggaagtg gttaaggaaa ccccagagta tcgctgcatg cagtcacagt tctctgtcct
     1501 gtataacgag agtctgcagt tgaaagccca cttggatgaa gctcggaccc tgcttcatgg
     1561 cacccggggt actcaccagc gccaggttga actcattgag cgagatgagg ttagtctgca
     1621 taagaagctg agaactgaag tcattcagct ggaggatacc ttggcccagg ttcgcaagga
     1681 gtatgagatg ttgaggatag agtttgagca gacccttgct gccaatgaac aagcaggccc
     1741 cataaaccgg gagatgcggc atctcattag cagcctccag aatcataacc accagctgaa
     1801 gggtgaagtc ctaaggtata aacggaagct gagagaggcg cagtctgacc tgaacaagac
     1861 acgtctgcgc agtggcagtg ccctcctgca gtctcagtct agtactgagg accccaagga
     1921 cgagcccaca gagctgaagc aggattctga agacctagcc actcattcct cagcgctgaa
     1981 ggcatctcag gaggatgaag ttaagtccaa acgggatgaa gaagagagag aacgagaaag
     2041 gagggagaaa gaaagagagc gagagcgaga acgagaaaag gaaaaggaga gagaacgaga
     2101 gaaacagaaa ctgaaagagt cagaaaagga gagagattct gttaaggata aagagaaagg
     2161 gaaacatgat gatggaagga agaaggaagc agaaattatc aagcagctga agattgaact
     2221 taagaaagca caagagagcc agaaggagat gaaactactg ctggatatgt accgctctgc
     2281 ccccaaggag cagagagaca aagtgcagct aatggcagct gagaagaagt caaaggcaga
     2341 gttggaggat ctcagacaaa gactcaagga tctggaagat aaggagaaga aagagaacaa
     2401 gaaaatggct gatgaggatg ccttgaggaa gatccgggca gtggaggagc agattgaata
     2461 cctgcagaag aagctggcta tggccaagca ggaggaagag gctctcctgt ctgagatgga
     2521 tgtcacagga caggcctttg aggacatgca ggagcagaac atccgtctga tgcagcagtt
     2581 gcgggagaag gacgatgcaa acttcaagct catgtcagag cgaatcaagt ctaatcagat
     2641 ccataagttg cttaaagagg aaaaggaaga gttagctgat caggttttga cactgaagac
     2701 tcaggtagat gcccagttac aggtagtaag aaaactggaa gagaaggagc acctacttca
     2761 gagtaacatt ggcacagggg agaaggagct gggtcttagg actcaagcct tggagatgaa
     2821 taaacgcaag gcaatggagg cggcccagct tgcagatgac ctcaaagcac agctagaact
     2881 ggctcagaag aagctccatg atttccagga tgaaatcgtg gagaacagtg tcaccaaaga
     2941 aaaggacttg ttcaatttca aacgagcaca ggaagacatc tctaggcttc gaagaaagct
     3001 agagaccacc aagaagccag acaatgtgcc caagtgtgat gaaattctga tggaggagat
     3061 aaaggactac aaggcacggc tgacctgccc ttgctgtaac atgcgtaaaa aggatgcagt
     3121 acttaccaag tgttttcacg ttttctgctt tgagtgtgtg aagacgcgct atgacactcg
     3181 ccagcgcaag tgtcccaagt gtaatgctgc ttttggtgcc aatgatttcc atcgcatcta
     3241 cattggctga ttgcagtcaa gaaagaggac tggctaggca agcacttact cattaatcac
     3301 caggcctcta cctcctctct cctagacagc tggccatctc ccttgttccc cagactttgt
     3361 ccgggttctc ttcgccagta gcgagagaac atagtgagga agactgcaag tcttgggact
     3421 tagaatgaac tagaaattac tagcgtttgt cttcccttca gctttctttg tgtccttctt
     3481 tcggattttt cagcattttc ttcttgggcc tagcctataa actggttttg cccactcctc
     3541 ttgaagaagt caggttgata gtttgggcac tgagaaggga atgtgggtag agtgaacata
     3601 tactattatg gaggagtgaa tttttgataa gttatctatg ggcccaaaat tcttgatttg
     3661 ggtagtaaac ctttctaaac ttaggttata aaatggtata gttgtgttag tctctcctgg
     3721 aaggtttgaa gccttaatga aattatatcc aggatacttc attctatttc tcacttctag
     3781 gagactgact ttttgggtca gttggacttc ttgctaaagt tatttatgtt ttaggtattc
     3841 aaatattaat agtttttttt taatttctta aagccagaat aatttcaaat gtgatagtcc
     3901 ctatggtgtg tgtttttttt ttttaatata catcttttct gaaatcagaa ttagtattaa
     3961 gaagtaacag gaatgcattg attgtgaaga gatctcggta gtctgggaat gagatccagg
     4021 gcgatgttgg ggaattatga attactgtcc cctcctctcc agagggagtc tttgttctct
     4081 gtgttcctga agattctcag taatgtttct gtaggatttt aatattccat gtgcttcaat
     4141 taaagcagga ttcaggttg
//
