LOCUS       NM_027187               2109 bp    mRNA    linear   ROD 08-FEB-2024
DEFINITION  Mus musculus ribonuclease H2, large subunit (Rnaseh2a), transcript
            variant 1, mRNA.
ACCESSION   NM_027187 XM_912989
VERSION     NM_027187.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Sugawara,S., Okada,R., Loo,T.M., Tanaka,H., Miyata,K., Chiba,M.,
            Kawasaki,H., Katoh,K., Kaji,S., Maezawa,Y., Yokote,K., Nakayama,M.,
            Oshima,M., Nagao,K., Obuse,C., Nagayama,S., Takubo,K.,
            Nakanishi,A., Kanemaki,M.T., Hara,E. and Takahashi,A.
  TITLE     RNaseH2A downregulation drives inflammatory gene expression via
            genomic DNA fragmentation in senescent and cancer cells
  JOURNAL   Commun Biol 5 (1), 1420 (2022)
   PUBMED   36577784
  REMARK    GeneRIF: RNaseH2A downregulation drives inflammatory gene
            expression via genomic DNA fragmentation in senescent and cancer
            cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Uehara,R., Cerritelli,S.M., Hasin,N., Sakhuja,K., London,M.,
            Iranzo,J., Chon,H., Grinberg,A. and Crouch,R.J.
  TITLE     Two RNase H2 Mutants with Differential rNMP Processing Activity
            Reveal a Threshold of Ribonucleotide Tolerance for Embryonic
            Development
  JOURNAL   Cell Rep 25 (5), 1135-1145 (2018)
   PUBMED   30380406
  REMARK    GeneRIF: An RNase H2 mutant lacking ribonucleotide excision repair
            activity but supporting R-loop removal revealed that
            ribonucleotides in DNA initiate p53-dependent DNA damage response
            and early embryonic arrest in mouse.
REFERENCE   3  (bases 1 to 2109)
  AUTHORS   Pokatayev,V., Hasin,N., Chon,H., Cerritelli,S.M., Sakhuja,K.,
            Ward,J.M., Morris,H.D., Yan,N. and Crouch,R.J.
  TITLE     RNase H2 catalytic core Aicardi-Goutieres syndrome-related mutant
            invokes cGAS-STING innate immune-sensing pathway in mice
  JOURNAL   J Exp Med 213 (3), 329-336 (2016)
   PUBMED   26880576
  REMARK    GeneRIF: the G37S mutation of RNaseH2 led to increased expression
            of interferon-stimulated genes dependent on the cGAS-STING
            signaling pathway.
REFERENCE   4  (bases 1 to 2109)
  AUTHORS   Rabe,B.
  TITLE     Aicardi-Goutieres syndrome: clues from the RNase H2 knock-out mouse
  JOURNAL   J Mol Med (Berl) 91 (11), 1235-1240 (2013)
   PUBMED   23744109
  REMARK    GeneRIF: Recent evidence gathered from ribonuclease H2 knock-out
            mice provides insight into the molecular mechanisms underlying
            Aicardi-Goutieres syndrome development and a potential role of DNA
            damage as a trigger of autoimmunity is discussed. [review]
            Review article
REFERENCE   5  (bases 1 to 2109)
  AUTHORS   Ghodgaonkar,M.M., Lazzaro,F., Olivera-Pimentel,M., Artola-Boran,M.,
            Cejka,P., Reijns,M.A., Jackson,A.P., Plevani,P., Muzi-Falconi,M.
            and Jiricny,J.
  TITLE     Ribonucleotides misincorporated into DNA act as
            strand-discrimination signals in eukaryotic mismatch repair
  JOURNAL   Mol Cell 50 (3), 323-332 (2013)
   PUBMED   23603115
REFERENCE   6  (bases 1 to 2109)
  AUTHORS   Reijns,M.A., Rabe,B., Rigby,R.E., Mill,P., Astell,K.R.,
            Lettice,L.A., Boyle,S., Leitch,A., Keighren,M., Kilanowski,F.,
            Devenney,P.S., Sexton,D., Grimes,G., Holt,I.J., Hill,R.E.,
            Taylor,M.S., Lawson,K.A., Dorin,J.R. and Jackson,A.P.
  TITLE     Enzymatic removal of ribonucleotides from DNA is essential for
            mammalian genome integrity and development
  JOURNAL   Cell 149 (5), 1008-1022 (2012)
   PUBMED   22579044
REFERENCE   7  (bases 1 to 2109)
  AUTHORS   Coffin,S.R., Hollis,T. and Perrino,F.W.
  TITLE     Functional consequences of the RNase H2A subunit mutations that
            cause Aicardi-Goutieres syndrome
  JOURNAL   J Biol Chem 286 (19), 16984-16991 (2011)
   PUBMED   21454563
  REMARK    GeneRIF: analysis of the RNase H2A subunit mutations that cause
            Aicardi-Goutieres syndrome
REFERENCE   8  (bases 1 to 2109)
  AUTHORS   Shaban,N.M., Harvey,S., Perrino,F.W. and Hollis,T.
  TITLE     The structure of the mammalian RNase H2 complex provides insight
            into RNA.NA hybrid processing to prevent immune dysfunction
  JOURNAL   J Biol Chem 285 (6), 3617-3624 (2010)
   PUBMED   19923215
  REMARK    GeneRIF: Data show that the interwoven architecture of RNase H2B
            and RNase H2C that interface with RNase H2A in a complex ideally
            suited for nucleic acid binding and hydrolysis coupled to
            protein-protein interaction motifs.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY098728.1 and AK146518.1.
            
            [WARNING] On Mar 31, 2024 this sequence was replaced by
            NM_027187.5.
            
            On Jun 15, 2018 this sequence version replaced NM_027187.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146518.1, BC038158.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BY098728.1         30-94
            66-2109             AK146518.1         2-2045
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.38 cM"
     gene            1..2109
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /note="ribonuclease H2, large subunit"
                     /db_xref="GeneID:69724"
                     /db_xref="MGI:MGI:1916974"
     exon            1..217
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..996
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /EC_number="3.1.26.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ribonuclease H2 subunit A; RNase H2 subunit A; RNase HI
                     large subunit; ribonuclease HI subunit A; ribonuclease HI
                     large subunit"
                     /codon_start=1
                     /product="ribonuclease H2 subunit A isoform 1"
                     /protein_id="NP_081463.1"
                     /db_xref="CCDS:CCDS22486.1"
                     /db_xref="GeneID:69724"
                     /db_xref="MGI:MGI:1916974"
                     /translation="MDLSELERDNTGRCRLSSPVPAVCLKEPCVLGVDEAGRGPVLGP
                     MVYAICYCPLSRLADLEALKVADSKTLTENERERLFAKMEEDGDFVGWALDVLSPNLI
                     STSMLGRVKYNLNSLSHDTAAGLIQYALDQNVNVTQVFVDTVGMPETYQARLQQHFPG
                     IEVTVKAKADSLFPVVSAASIFAKVARDKAVKNWQFVENLQDLDSDYGSGYPNDPKTK
                     AWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAEDVIWEDSEAEEDPERPGKITSYFSQ
                     GPQTCRPQAPHRYFQERGLEAASSL"
     exon            218..289
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            290..416
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            417..504
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            505..642
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            643..730
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            731..854
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
     exon            855..2109
                     /gene="Rnaseh2a"
                     /gene_synonym="2400006P09Rik; RNASEHI; RNHIA; RNHL"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagagccact gcagttttcg cggaaaacgc gcgctgggac ctgcgcttgc agtgttgttt
       61 tctacaattg ttggctgtcg cagaggcagc atggatctca gcgagctgga gagggacaat
      121 acgggtcgtt gtcgtctgag ttctcctgta cctgctgtgt gtctcaagga gccgtgcgtt
      181 ctgggcgtgg atgaagcggg ccgaggccct gtgcttggtc ccatggtcta cgccatctgt
      241 tactgccccc tgtctcgctt ggcagatctg gaggccctga aagtggcaga ctctaagacc
      301 ttgacagaga acgagcggga gaggctcttt gcgaaaatgg aggaggatgg agactttgtg
      361 ggttgggctt tggacgtcct gtctccaaac ctgatctcta ccagcatgct tgggcgagtc
      421 aagtacaacc tcaactccct gtcacacgat acagctgcgg ggctgataca gtacgcactg
      481 gaccagaatg tgaatgtcac tcaggtattt gtggacactg taggaatgcc agagacatac
      541 caggctcgat tacaacagca ctttcccggg atagaggtga cagtcaaggc caaagctgac
      601 tccctgttcc ctgtggtcag tgctgccagc atctttgcca aggtggcccg agacaaggct
      661 gtgaagaact ggcagtttgt ggaaaattta caggatctgg actccgatta tggctcaggc
      721 tatcccaatg atcccaagac caaagcctgg ctgaggaaac atgtggaccc tgtgtttggc
      781 tttccccagt ttgtacggtt cagttggagc acagcccagg ccatcctgga gaaggaggca
      841 gaagatgtca tttgggagga ctcagaagct gaggaggatc ctgaaagacc gggaaaaatc
      901 acctcctact tcagccaggg cccgcagacc tgccgccctc aggcccccca cagatacttc
      961 caggagcgag gcctggaggc agccagcagc ctctaggagc ccacgtgtgc accacctgcc
     1021 cttctaaccc aggaataaaa gctgttcaag aagtggaccg agtgctttgt ggctaaggtc
     1081 tggtagcagt gcctgtggcc agcagctgat tcatcaaaca aaataggcat ttctagattg
     1141 gtgacgtttg cttttttttt aaagaatgat ttatgtatgt aagtacactg tagctgtctt
     1201 cagacacggc agaagagggc atcagatccc attatagatg gttgtgagcc accatgtggt
     1261 atcactggat agctcaatgc tggaactgtg tagaccaggt tggtcttggg cacattgtgg
     1321 cactcacctg cctctgcctc ccaagtgctg aggttaaaga catgcactat catagccttt
     1381 ttattttttt taattgtaaa actatttttg tctttgtgtg ggtatacaca catgtgagta
     1441 tgcacgtgta aatgcaaggg cccttggagg tcgtaggctt cagatttctc tggagctgga
     1501 cttagagaaa ttacggcctg ctctgttggt gctgatctga actatgtgct tttgaccact
     1561 ggctttctcc atcccgcata tttttctttg gtgctccctc tttgctttgt cttgttttga
     1621 gacagggttc ctaagtagtc ttgactgtcc tggaacttgc tacgtagacc agacaggcct
     1681 gagacacagc tgcagacttg gaattaaagg agttgtatgt atatttgtgc aacacatcca
     1741 tgcagttctc aaagaagcca gaagagggca ccagatcccc tgcaactgga gttggaggtg
     1801 tttttgagcc accttgtggg tcttgagaat caggtcctct gcaagaacag tcattgttgc
     1861 tgtcaaccag ggagccatct ttccagtcct ctgcctcctc tcctcttcct tctgtgtgtg
     1921 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtttg cgtgcatgcg ctgttgtggt
     1981 attgcatacc tttaatccca gcatttggag gacaggtagg tagatctctg agttagaggc
     2041 cattctggtc tacatagttc cacatcagtc aaggctacat gatgaggccc tgatcaataa
     2101 atcaaagcc
//
