LOCUS       NM_011275               1472 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus ribonuclease H1 (Rnaseh1), transcript variant 1, mRNA.
ACCESSION   NM_011275
VERSION     NM_011275.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Jiang,Y., Huang,F., Chen,L., Gu,J.H., Wu,Y.W., Jia,M.Y., Lin,Z.,
            Zhou,Y., Li,Y.C., Yu,C., Tong,M.H., Shen,L., Fan,H.Y. and Sha,Q.Q.
  TITLE     Genome-wide map of R-loops reveals its interplay with transcription
            and genome integrity during germ cell meiosis
  JOURNAL   J Adv Res 51, 45-57 (2023)
   PUBMED   36396044
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Liu,C., Wang,L., Li,Y., Guo,M., Hu,J., Wang,T., Li,M., Yang,Z.,
            Lin,R., Xu,W., Chen,Y., Luo,M., Gao,F., Chen,J.Y., Sun,Q., Liu,H.,
            Sun,B. and Li,W.
  TITLE     RNase H1 facilitates recombinase recruitment by degrading DNA-RNA
            hybrids during meiosis
  JOURNAL   Nucleic Acids Res 51 (14), 7357-7375 (2023)
   PUBMED   37378420
REFERENCE   3  (bases 1 to 1472)
  AUTHORS   Misic,J., Milenkovic,D., Al-Behadili,A., Xie,X., Jiang,M.,
            Jiang,S., Filograna,R., Koolmeister,C., Siira,S.J., Jenninger,L.,
            Filipovska,A., Clausen,A.R., Caporali,L., Valentino,M.L., La
            Morgia,C., Carelli,V., Nicholls,T.J., Wredenberg,A., Falkenberg,M.
            and Larsson,N.G.
  TITLE     Mammalian RNase H1 directs RNA primer formation for mtDNA
            replication initiation and is also necessary for mtDNA replication
            completion
  JOURNAL   Nucleic Acids Res 50 (15), 8749-8766 (2022)
   PUBMED   35947649
  REMARK    GeneRIF: Mammalian RNase H1 directs RNA primer formation for mtDNA
            replication initiation and is also necessary for mtDNA replication
            completion.
REFERENCE   4  (bases 1 to 1472)
  AUTHORS   Lai,F., Damle,S.S., Ling,K.K. and Rigo,F.
  TITLE     Directed RNase H Cleavage of Nascent Transcripts Causes
            Transcription Termination
  JOURNAL   Mol Cell 77 (5), 1032-1043 (2020)
   PUBMED   31924447
REFERENCE   5  (bases 1 to 1472)
  AUTHORS   Suzuki,Y., Holmes,J.B., Cerritelli,S.M., Sakhuja,K., Minczuk,M.,
            Holt,I.J. and Crouch,R.J.
  TITLE     An upstream open reading frame and the context of the two AUG
            codons affect the abundance of mitochondrial and nuclear RNase H1
  JOURNAL   Mol Cell Biol 30 (21), 5123-5134 (2010)
   PUBMED   20823270
  REMARK    GeneRIF: translational organization of RNase H1 allows tight
            control of expression of RNase H1 in mitochondria, where its excess
            or absence can lead to cell death, without affecting the expression
            of the nuclear RNase H1
REFERENCE   6  (bases 1 to 1472)
  AUTHORS   Cerritelli,S.M. and Crouch,R.J.
  TITLE     Ribonuclease H: the enzymes in eukaryotes
  JOURNAL   FEBS J 276 (6), 1494-1505 (2009)
   PUBMED   19228196
  REMARK    Review article
REFERENCE   7  (bases 1 to 1472)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 1472)
  AUTHORS   Gaidamakov,S.A., Gorshkova,I.I., Schuck,P., Steinbach,P.J.,
            Yamada,H., Crouch,R.J. and Cerritelli,S.M.
  TITLE     Eukaryotic RNases H1 act processively by interactions through the
            duplex RNA-binding domain
  JOURNAL   Nucleic Acids Res 33 (7), 2166-2175 (2005)
   PUBMED   15831789
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1472)
  AUTHORS   Cerritelli,S.M., Frolova,E.G., Feng,C., Grinberg,A., Love,P.E. and
            Crouch,R.J.
  TITLE     Failure to produce mitochondrial DNA results in embryonic lethality
            in Rnaseh1 null mice
  JOURNAL   Mol Cell 11 (3), 807-815 (2003)
   PUBMED   12667461
  REMARK    GeneRIF: RNase H1 is involved in generation of mitochondrial DNA
REFERENCE   10 (bases 1 to 1472)
  AUTHORS   Cerritelli,S.M. and Crouch,R.J.
  TITLE     Cloning, expression, and mapping of ribonucleases H of human and
            mouse related to bacterial RNase HI
  JOURNAL   Genomics 53 (3), 300-307 (1998)
   PUBMED   9799596
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY274268.1, CA554498.1,
            BM943446.1, DV662103.1 and AI390696.1.
            
            On Nov 19, 2013 this sequence version replaced NM_011275.2.
            
            Summary: This gene encodes an endonuclease that specifically
            degrades the RNA of RNA-DNA hybrids and is necessary for DNA
            replication and repair. This enzyme is present in both mitochondria
            and nuclei, which are resulted from translation of a single mRNA
            with two in-frame initiation start codons. The use of the first
            start codon produces the mitochondrial isoform and the use of the
            second start codon produces the nuclear isoform. The production of
            the mitochondrial isoform is modulated by an upstream open reading
            frame (uORF) which encodes 7aa in mouse. An alternately spliced
            transcript variant has been found which is a candidate for
            nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (1) encodes two isoforms due to
            the use of alternative translation initiation codons. The longer
            isoform (1) is derived from the upstream AUG start codon, while the
            shorter isoform (2) is derived from the downstream AUG start codon.
            This RefSeq represents the longer isoform (1), which is a
            mitochondrial protein (see details in PMID: 20823270).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF048993.1, BI330591.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 20823270; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            regulatory uORF                    :: PMID: 20823270
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               BY274268.1         2-129
            129-572             CA554498.1         80-523
            573-1137            BM943446.1         93-657
            1138-1452           DV662103.1         356-670
            1453-1472           AI390696.1         198-217
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 10.99 cM"
     gene            1..1472
                     /gene="Rnaseh1"
                     /note="ribonuclease H1"
                     /db_xref="GeneID:19819"
                     /db_xref="MGI:MGI:1335073"
     exon            1..215
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      61..84
                     /regulatory_class="uORF"
                     /gene="Rnaseh1"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:20823270]"
                     /note="this uORF is predicted to encode a 7 aa peptide
                     (PMID:20823270)"
     exon            216..331
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            332..493
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            494..593
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            594..648
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            649..733
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            734..858
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1457
                     /gene="Rnaseh1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1430..1435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnaseh1"
                     /note="hexamer: AATAAA"
     polyA_site      1457
                     /gene="Rnaseh1"
                     /note="major polyA site"
ORIGIN      
        1 gacgtcacct tcctgagccg gaagtgtgtc ggctcgccgg cgggaggctc gcgggcagca
       61 atgtgggttg ccgcgctggc gtgagtgatg cgctggctgc tgccgctgtc ccgcacagtg
      121 acactggccg tcgtacgcct gaggcgaggc atttgcgggc tcgggatgtt ctatgcggtg
      181 aggagaggcc gcaggaccgg agtcttcctg agttggagtg agtgcaaagc ccaggtggac
      241 cggtttcctg ctgccaggtt taagaaattt gccacagaag atgaggcctg ggcctttgtc
      301 aggagctctt caagcccgga tggttcaaaa gggcaggaaa gtgcacatga gcagaagtca
      361 caggcgaaga ccagcaagcg gcctcgggag cccctgggtg aaggggaaga acttccagag
      421 ccagggccga agcacacacg acaggacacc gagccctcgg ctgtagtgag caaggacgca
      481 ttttcttata tgggagagtc agtcgttgtc tacacggatg gctgttgctc cagtaatgga
      541 cggaagcggg cacgagcagg aattggcgtt tactggggcc caggccaccc cttaaatgta
      601 ggtataaggc ttcctgggcg acagacaaac cagagggccg agatccatgc agcctgcaag
      661 gccatcatgc aagccaaggc tcagaacatc agcaagctgg ttctgtacac agacagcatg
      721 ttcaccatca atgggataac taactgggtt cagggctgga agaagaatgg ctggagaaca
      781 agtacaggga aagatgtgat caacaaggag gacttcatgg agctggacga gctcactcag
      841 ggcatggaca tccagtggat gcacattcct ggtcactcag gatttgtggg caatgaagag
      901 gccgacagac tggcacggga aggagcgaag cagtctgagg actgagcaga ggagctgctg
      961 ccgactcaga cccaaggcag aggctgtttc tttaccctgc cgtgcactga gctgtgcatg
     1021 gaggacacag tgtaacctgt ggcacagctt ggtgagacgt gcttacataa tggaaaggaa
     1081 gtgttaaatc ctgcttttgt gagatttcag ggtctgtccc aaaatgctcc agctggattt
     1141 tagtaggagt cagtgagacc ctggtcagta cgtgtggcct aaaagttcca gaaggcacaa
     1201 gtgcaagact cctgcccctg cagctgccct ctatctctgt gcatctgaga taatgtggcc
     1261 acgtgggaca cagcctacac tcaggtggcc ttgactcctg gatcaccttg aaggaacagc
     1321 tgagaccttt aaaaatcaat atattactaa tagatttctt tgcctatttt ttcttaaaaa
     1381 tactgtataa atttaaaaca tgttgaaatt atccttattt attttttata ataaaagtat
     1441 ataggaatga cacagaaaaa aaaaaaaaaa aa
//
