LOCUS       NM_029679               5598 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus RHO family interacting cell polarization regulator 2
            (Ripor2), transcript variant 1, mRNA.
ACCESSION   NM_029679
VERSION     NM_029679.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Li,J., Liu,C., Muller,U. and Zhao,B.
  TITLE     RIPOR2-mediated autophagy dysfunction is critical for
            aminoglycoside-induced hearing loss
  JOURNAL   Dev Cell 57 (18), 2204-2220 (2022)
   PUBMED   36113482
  REMARK    GeneRIF: RIPOR2-mediated autophagy dysfunction is critical for
            aminoglycoside-induced hearing loss.
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Ishiba,R., Santos,A.L.F., Almeida,C.F., Caires,L.C. Jr.,
            Ribeiro,A.F. Jr., Ayub-Guerrieri,D., Fernandes,S.A., Souza,L.S. and
            Vainzof,M.
  TITLE     Faster regeneration associated to high expression of Fam65b and
            Hdac6 in dysferlin-deficient mouse
  JOURNAL   J Mol Histol 50 (4), 375-387 (2019)
   PUBMED   31218594
  REMARK    GeneRIF: dysferlin deficiency seems to significantly alter the gene
            expression of Fam65b and Hdac6 during regeneration, since higher
            levels of expression of both genes were observed in
            dysferlin-deficient mice.
REFERENCE   3  (bases 1 to 5598)
  AUTHORS   Zhou,L.Y., Zhai,M., Huang,Y., Xu,S., An,T., Wang,Y.H., Zhang,R.C.,
            Liu,C.Y., Dong,Y.H., Wang,M., Qian,L.L., Ponnusamy,M., Zhang,Y.H.,
            Zhang,J. and Wang,K.
  TITLE     The circular RNA ACR attenuates myocardial ischemia/reperfusion
            injury by suppressing autophagy via modulation of the Pink1/ FAM65B
            pathway
  JOURNAL   Cell Death Differ 26 (7), 1299-1315 (2019)
   PUBMED   30349076
  REMARK    GeneRIF: The FAM65B is a downstream target of Pink1 and Pink1
            phosphorylates FAM65B at serine 46. Phosphorylated FAM65B inhibits
            autophagy and cell death in the heart.
REFERENCE   4  (bases 1 to 5598)
  AUTHORS   Diaz-Horta,O., Abad,C., Cengiz,F.B., Bademci,G., Blackwelder,P.,
            Walz,K. and Tekin,M.
  TITLE     Ripor2 is involved in auditory hair cell stereociliary bundle
            structure and orientation
  JOURNAL   J Mol Med (Berl) 96 (11), 1227-1238 (2018)
   PUBMED   30280293
  REMARK    GeneRIF: We propose that Ripor2 deficiency influences abundance
            and/or post-translational modifications of proteins expressed in
            both stereocilia and kinocilia. This effect may have a negative
            impact on the structure and function of the auditory hair cell
            bundle.
REFERENCE   5  (bases 1 to 5598)
  AUTHORS   Megrelis,L., El Ghoul,E., Moalli,F., Versapuech,M., Cassim,S.,
            Ruef,N., Stein,J.V., Mangeney,M. and Delon,J.
  TITLE     Fam65b Phosphorylation Relieves Tonic RhoA Inhibition During T Cell
            Migration
  JOURNAL   Front Immunol 9, 2001 (2018)
   PUBMED   30254631
  REMARK    GeneRIF: results show that Fam65b expression and phosphorylation
            can finely tune the amount of active RhoA in order to favor optimal
            T lymphocyte motility.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5598)
  AUTHORS   Diaz-Horta,O., Subasioglu-Uzak,A., Grati,M., DeSmidt,A., Foster,J.
            2nd, Cao,L., Bademci,G., Tokgoz-Yilmaz,S., Duman,D., Cengiz,F.B.,
            Abad,C., Mittal,R., Blanton,S., Liu,X.Z., Farooq,A., Walz,K., Lu,Z.
            and Tekin,M.
  TITLE     FAM65B is a membrane-associated protein of hair cell stereocilia
            required for hearing
  JOURNAL   Proc Natl Acad Sci U S A 111 (27), 9864-9868 (2014)
   PUBMED   24958875
REFERENCE   7  (bases 1 to 5598)
  AUTHORS   Balasubramanian,A., Kawahara,G., Gupta,V.A., Rozkalne,A.,
            Beauvais,A., Kunkel,L.M. and Gussoni,E.
  TITLE     Fam65b is important for formation of the HDAC6-dysferlin protein
            complex during myogenic cell differentiation
  JOURNAL   FASEB J 28 (7), 2955-2969 (2014)
   PUBMED   24687993
REFERENCE   8  (bases 1 to 5598)
  AUTHORS   Yoon,S., Molloy,M.J., Wu,M.P., Cowan,D.B. and Gussoni,E.
  TITLE     C6ORF32 is upregulated during muscle cell differentiation and
            induces the formation of cellular filopodia
  JOURNAL   Dev Biol 301 (1), 70-81 (2007)
   PUBMED   17150207
REFERENCE   9  (bases 1 to 5598)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   10 (bases 1 to 5598)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY123481.1, AK039271.1, AK031928.1, BC059179.1 and AA190042.1.
            
            On Oct 30, 2013 this sequence version replaced NM_029679.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122269.1, AK134538.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                BY123481.1         1-36
            37-48               AK039271.1         2-13
            49-2187             AK031928.1         2-2140
            2188-5525           BC059179.1         2057-5394
            5526-5598           AA190042.1         48-120
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 10.5 cM"
     gene            1..5598
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /note="RHO family interacting cell polarization regulator
                     2"
                     /db_xref="GeneID:193385"
                     /db_xref="MGI:MGI:2444879"
     exon            1..289
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..3477
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein FAM65B; family with sequence similarity 65, member
                     B"
                     /codon_start=1
                     /product="rho family-interacting cell polarization
                     regulator 2 isoform 1"
                     /protein_id="NP_083955.1"
                     /db_xref="CCDS:CCDS36622.1"
                     /db_xref="GeneID:193385"
                     /db_xref="MGI:MGI:2444879"
                     /translation="MPPGTKRLRALGAFSAGLPTRLPEIMLVGSQSFSPGGPNGIIRS
                     QSFAGFSGLQERRSRCNSFIENASALKKPQAKLKKMHNLGHKNNNTPKEPQPKRVEEV
                     YRALKNGLDEYLEFHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFH
                     ISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLSEINRSYKEYTENMCAI
                     EAELESLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGA
                     ETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKL
                     NLEITWYPFDVEDTTPSSGPGNKTAALQRRMSMYSQGTPETPTFKDQSFFRWLRLSVL
                     SALRDTFFATLHHNHSVGDLPSLSLNPKALLEFYSNLPDDIFESGKAAEEKRPLSLSF
                     SDLQDGDCVFTSSSATSPSSSHSAHPEITITPAELTHSSLSSQNEGTEDSSSASSRNS
                     LGEDHEPKSHPKSDTVEPGKPGVATRSGTESLFLESSVAEALLQESDEASELKPVELD
                     TFEGNITKQLVKRLTSAEGPVTTDKLFFEGSVGSESEAGRSFLDGSLEDAFNGLFLAL
                     DPHKKQYKEFQDLNQEVTHLDDVLKCKPAGSRSRSSSLSLTVESALESFDFLNTSDFD
                     EEEEDGDDVCHVGGGADSVFSDTETEKSGYRSVHPEARGHLSEALTEDTGVGTSVAGS
                     PLPLTTGNESLDITIVKHLQYCTQLIQQIVFSSKTPFVARSLLEKLSRQVLVMQKLAA
                     VSDENLGNITSVVEAIPEFHKKLSLLAFWTKCCSPSGVYHSSAARLIKQLEASFARSI
                     NKDYPGLAEPVFRTLVSQILDRAEPLLSSSLSSEVITVFQYYSFFTSHGVSDLETYLG
                     QLTRQVAMVQTLQSLRDEKLLQTMSDLAPSNLPAQQEVLRTLALLLTRDDNEVSEAVT
                     LYLAAASKNEHFREKALLYYCEALTKANLQLQKAACLALKSLEATESIKMLVTLCQSD
                     TEEIRTVASETLLSLGEDGRLAYEQLDKFPRDCVKVGGRHGTEVATAF"
     exon            290..416
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            417..572
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            573..651
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            652..675
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            676..729
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            730..879
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            880..943
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            944..987
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1113
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1262
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1392
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1518
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..2187
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2372
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2541
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2677
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2839
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..2998
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3201
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3285
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3376
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     exon            3377..5598
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5575..5580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /note="hexamer: AATAAA"
     polyA_site      5598
                     /gene="Ripor2"
                     /gene_synonym="Fam65b"
                     /note="major polyA site"
ORIGIN      
        1 gcgtgcgcgc ctccccaccg ccctctccgc taccgcgctc gctccgctct ctgccacccg
       61 cgcgtcctct ggccccgcaa cttcacccgg cgcggcggca gctggtgcga cgcccgagcg
      121 ctggggccac gactgttacc ctcggacctt tccctgttgg ctgggaggaa cgagcaccgg
      181 tgtccgcgga gccggaggtg cacggcagcg aggggcggtg cgtgggtgcc gaggtggtac
      241 atgccacccg gtactaagcg tctccgggca ctaggagcgt tctcggcagg actgccaacc
      301 agactcccag aaatcatgct ggtgggatcc cagtccttct ctcctggcgg gcccaatggc
      361 atcatccgga gccagtcctt cgcaggtttc agtgggctgc aggaaaggcg gtccaggtgt
      421 aactccttca ttgaaaatgc ctcggctctc aagaagcctc aggccaagct gaagaaaatg
      481 cataatcttg gacacaaaaa taacaacact cccaaggagc ctcagccgaa gagagtggag
      541 gaggtctacc gggccttgaa gaacgggctt gatgagtacc tggagtttca ccagacggag
      601 ctggacaaat tgactgctca attaaaagat atgaagagaa actctcgcct gggtgtgctg
      661 tatgacctag acaagcaaat taaaacgatt gagagataca tgcgacgcct ggagtttcac
      721 attagtaagg tagacgagct ctatgaagcc tattgtatcc aacggcgtct ccaagatggt
      781 gccagcaaaa tgaagcaagc cttcgcgacg tcccctgcca gcaaggctgc ccgggagagt
      841 ctgtctgaga tcaaccgcag ctacaaggaa tacacggaga acatgtgtgc cattgaagcg
      901 gagttggaga gtctgctggg agaattctcc atcaagatga aaggtctggc tggctttgcc
      961 cgcctctgtc ctggagatca atatgaaatt ttcatgaagt atggtcggca gaggtggaaa
     1021 ctgaagggca aaatagaagt gaatggtaaa cagagctggg atggagcaga aaccgtcttc
     1081 ctgcccctca tagttgggtt catttcaatc aaggtcacag aactcaaagg actggcgact
     1141 cacatcctgg taggcagtgt gacctgcgag accaaagagc tgtttgcagc ccgaccccag
     1201 gtggtggctg ttgacatcaa tgaccttggt accatcaaac tgaacctgga aatcacctgg
     1261 tatccctttg acgtggagga cacaacccca tcgtcgggcc ctgggaacaa gacagctgct
     1321 ctccagcgaa ggatgtccat gtacagccag ggcaccccag aaacacctac cttcaaagac
     1381 cagtccttct ttagatggtt gcggctgtct gtcttgagtg ccttgcgaga cactttcttt
     1441 gccacgttgc accacaacca ctctgtcggt gacctgcctt ccctcagcct gaaccccaag
     1501 gccctgctag aattctattc aaatctgcca gatgacatct ttgagagtgg aaaggctgca
     1561 gaggagaaaa ggccactgtc cctcagcttc agtgacctgc aggatggaga ctgtgtcttc
     1621 acctccagct cagccacctc cccctccagt tcacactcag cccatccaga gattaccatc
     1681 acccccgcag agcttaccca cagcagcctg tcctcacaaa atgagggcac cgaggacagc
     1741 agctcagcgt cttccaggaa ttccttgggt gaggaccatg agcccaagtc ccacccaaag
     1801 agtgatacgg tagagcccgg gaagccgggt gtggctacaa ggtctggtac cgagagcctg
     1861 tttctggaga gtagtgtggc cgaggctctc ctgcaggaat cggatgaggc ctctgagctc
     1921 aagcccgtgg aactggacac ttttgaagga aacatcacaa agcaactggt gaagaggctc
     1981 acctcggctg aggggcccgt cactactgac aagcttttct ttgaaggctc tgttggtagc
     2041 gagtctgagg ctggccggtc ctttctggat ggcagcctgg aagatgcctt caatggactc
     2101 ttccttgcat tagacccaca caagaagcag tacaaagagt tccaggatct gaaccaagaa
     2161 gtcactcact tggatgatgt tctcaaatgc aagccggctg gcagccgcag caggtcttcc
     2221 agcctaagtc tgacggttga gagtgcttta gaaagctttg atttcctcaa cacctctgac
     2281 tttgacgaag aggaagagga tggcgatgac gtctgccacg ttggaggagg cgcagactcc
     2341 gtgttttcag atactgagac tgagaagagt ggttacaggt cagttcaccc ggaagccagg
     2401 ggtcacctta gcgaggctct gactgaagac acaggtgtag gcaccagtgt ggcaggaagc
     2461 ccgctcccac tcaccacagg aaatgagagc ctggacatca ccatagtcaa acatctacaa
     2521 tactgcaccc agctcatcca gcaaatcgtc ttctctagca aaaccccctt tgtggcaaga
     2581 agtctcttgg agaagctgtc gaggcaggtc cttgtgatgc agaagctggc agccgtgagt
     2641 gacgagaacc tcgggaacat cacctctgtg gtggaagcca taccagaatt tcacaaaaag
     2701 ctctccttgc tggccttctg gaccaaatgc tgcagcccca gcggggtcta ccacagctcc
     2761 gctgcccgcc tcatcaagca gctggaggcc agcttcgcca gaagcatcaa caaagactat
     2821 ccaggactgg cagagccagt gtttagaacc ctggtgtccc aaatcctgga ccgagctgaa
     2881 cctctccttt cctccagcct ctcgtcggaa gtcatcactg tcttccagta ctacagcttc
     2941 ttcaccagcc acggtgtgag tgacctggag acttacctgg gccagctaac caggcaagtt
     3001 gccatggttc agactctgca gtcactgaga gatgagaaac tgctgcagac tatgagcgac
     3061 cttgcaccca gcaacctccc cgctcaacag gaagtcctca ggacgctggc cctgctgctc
     3121 accagggatg acaatgaggt cagcgaagct gtgacactct acttggcagc agcctccaaa
     3181 aatgagcact tcagagaaaa ggccttgctc tattattgcg aagcactaac gaaggccaat
     3241 ctccagctac agaaggcagc atgtctggcc ctaaagagcc tggaggccac cgaaagcatt
     3301 aaaatgctag tgacgttgtg tcagtctgac actgaagaaa tcagaaccgt ggcctcggaa
     3361 accctcctct ctcttggaga agacgggcga ctggcgtacg agcaattaga caaattccct
     3421 cgagattgtg ttaaagtggg aggtcgtcat ggaaccgaag ttgccacagc cttttaaatt
     3481 tcacgttaac cctgcctgac tgctattccg atgatacctg gcccctgttc tcaagagggt
     3541 gctgtgatgt ggccagacag cagagagctg ctgccgctgc caagacggag atgtccaagt
     3601 ccaattgcac tgtactgtct acttttcctt agcccacaga acagggctgt gtataactcc
     3661 ctggactgca tcgtagtttt gactgacagc acttcaaagt agagtttgaa agtagtcatc
     3721 tttatgcctt tgtggaagtt catctgtaag cttgaattct tcatgtgcta tttggataca
     3781 tacatatcgt atgccttgcc gtctttctgc tgtgttctca gggttgagtg gccccttagt
     3841 taccgaattt tattaaacag acaaagcaca aaaggaatgt actgcaaata aaagttgttg
     3901 tttttttttt ttatttttac agagcctggc aaaagtgatc agctataaga cagtaagttt
     3961 tggagctgag ggtgtggctc ccttggtaga gtgctagcct agggagcagg aagcttgggt
     4021 ctatccccag cactgcacaa gctggctatg ctgcgcatat aaaatcagtg ctcagaagag
     4081 aagaaggatc agaagaagtt caaggtcatc ttcagctacc tagggagttc ccagggcaac
     4141 ctgggataca tgaagctatg ggggttgagg agacctaagt tttctgtttt tgtttttgtt
     4201 tttgcttttt tggttttttg agacagggtt tctctgtgta gccctggctg tcctggaact
     4261 cactctgtag accaggctgg cctcgaactc agaaatctgc ctgcctctgc ctcccaagtg
     4321 ctgggatcaa aggcatgcgc caccacgccc ggctgggacc taagttttat agcttagttt
     4381 tttatagttc ctactatttg tttctatttc catcctgaga atcccatatc atctatccat
     4441 gtggtttaag agaggaacgg aagaattctg cttcgttgag gttgtgccta tactattaaa
     4501 agtaagcaaa accttaagct ttcgttcttt aaccagagcc atctttatca cactcttggt
     4561 tttttgttgt ttggcttttg ttttgtcttg aaacagagtc ttcctattgt agtcctgacc
     4621 aacctggaac tcaatataca gtccagcctg acctcgaact tgcaagggtt ctccagcctc
     4681 tgcctccaga gctggggtta taggcacaag ccactaggcc tggttctaca tgtgtaattt
     4741 ttttttctaa gacaaataca cacgcctaag ttgtagattc agaaagatgt atttctcttt
     4801 taagtgaaaa cctggacttt ggatgaatcc taagtcactg gactacattg tagttgtgac
     4861 tgacagcact tcaaagtcca tcttacctta ccgtagaatt tgacactgga cagaatattg
     4921 aggtactgaa agtggttcct gcttctatag aatagccatg aaccttggaa gcaggatgcc
     4981 ctgagagatg gaaactgccc catagtttcc acagcagctc agtaccaggg tagagattca
     5041 gtctcttaca acgtgtgcaa ggagtgggga tgccaagaca tcacaataat atgtgtgggt
     5101 gcaatgtgaa ctgtattctg gatgtgtttg tataaaatac caattagata ttagttccaa
     5161 tatttacatt tcagcaagac ttttaaaagt tcctttcatt ttaaagtgaa aattgttcgc
     5221 tgggcttctt gcaacacatc ctttcaactg tgaagtccca ctgtacatat ctgtattttt
     5281 gtaaatgtta tatatatgca tatatccctc attttaaaag tatgttgtac agttatagta
     5341 tgtataggct acagtctttg ttaaatggtt gaaatgtctc ttttgtagaa agtgaaatca
     5401 cagctattac agggcttttg tttggtttat ttgtggattt gaaacagttt tgaatattgc
     5461 atggataatt aatttcacat cttttgtatc cacctctgtg tctggctttt ggttgggaag
     5521 tgcttttagg tttgtgatat ttgtattcat ccctctttca atcatgtaag tcaaaataaa
     5581 aattattttt aattacta
//
