LOCUS       NM_025934               3201 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus RIO kinase 2 (Riok2), mRNA.
ACCESSION   NM_025934
VERSION     NM_025934.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Messling,J.E., Pena-Romer,I., Moroni,A.S., Bruestl,S. and Helin,K.
  TITLE     RIO-kinase 2 is essential for hematopoiesis
  JOURNAL   PLoS One 19 (4), e0300623 (2024)
   PUBMED   38564577
  REMARK    GeneRIF: RIO-kinase 2 is essential for hematopoiesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3201)
  AUTHORS   Messling,J.E., Agger,K., Andersen,K.L., Kromer,K., Kuepper,H.M.,
            Lund,A.H. and Helin,K.
  TITLE     Targeting RIOK2 ATPase activity leads to decreased protein
            synthesis and cell death in acute myeloid leukemia
  JOURNAL   Blood 139 (2), 245-255 (2022)
   PUBMED   34359076
  REMARK    GeneRIF: Targeting RIOK2 ATPase activity leads to decreased protein
            synthesis and cell death in acute myeloid leukemia.
REFERENCE   4  (bases 1 to 3201)
  AUTHORS   Raundhal,M., Ghosh,S., Myers,S.A., Cuoco,M.S., Singer,M.,
            Carr,S.A., Waikar,S.S., Bonventre,J.V., Ritz,J., Stone,R.M.,
            Steensma,D.P., Regev,A. and Glimcher,L.H.
  TITLE     Blockade of IL-22 signaling reverses erythroid dysfunction in
            stress-induced anemias
  JOURNAL   Nat Immunol 22 (4), 520-529 (2021)
   PUBMED   33753942
  REMARK    Erratum:[Nat Immunol. 2021 Nov;22(11):1465. doi:
            10.1038/s41590-021-01031-y.. PMID: 34522041]
REFERENCE   5  (bases 1 to 3201)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3201)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   7  (bases 1 to 3201)
  AUTHORS   LaRonde-LeBlanc,N. and Wlodawer,A.
  TITLE     The RIO kinases: an atypical protein kinase family required for
            ribosome biogenesis and cell cycle progression
  JOURNAL   Biochim Biophys Acta 1754 (1-2), 14-24 (2005)
   PUBMED   16182620
  REMARK    Review article
REFERENCE   8  (bases 1 to 3201)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 3201)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY133202.1, AC154200.1, AK008451.1, BY710172.1 and AI553565.1.
            
            On Dec 11, 2009 this sequence version replaced NM_025934.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049232.1, BC010781.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               BY133202.1         1-139
            140-140             AC154200.1         109513-109513
            141-352             BY133202.1         141-352
            353-2131            AK008451.1         113-1891
            2132-2653           AC154200.1         128876-129397
            2654-3194           BY710172.1         56-596
            3195-3201           AI553565.1         1-7                 c
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 9.77 cM"
     gene            1..3201
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="RIO kinase 2"
                     /db_xref="GeneID:67045"
                     /db_xref="MGI:MGI:1914295"
     exon            1..339
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             274..1917
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase RIO2"
                     /protein_id="NP_080210.1"
                     /db_xref="CCDS:CCDS28417.1"
                     /db_xref="GeneID:67045"
                     /db_xref="MGI:MGI:1914295"
                     /translation="MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPCSLIASIASL
                     KHGGCNKILRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMG
                     VGKESDIYIVANEAGQQLALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMK
                     EFAYMKALYERKFPVPKPIDYNRHAVIMELINGYPLCQIHHVEDPASVYDEAMELIVK
                     LGNHGLIHGDFNEFNLMLDKDDHITMIDFPQMVSTSHPNAEWYFDRDVKCIREFFMKR
                     FSYESELYPTFSDIRKEDSLDVEVSASGYTKEMQADDELLHPVGPDDKITETEEDSDF
                     TFSDEEMLEKAKVWRSELEKEADPADESGGSWCCSSTDSKQIKDGGLPEESAHVSSFE
                     VTALSQAVEEMERQVLPHRSVTEFSEESRRTENDGQPGQRSPAGSEDCDDEPPHLIAL
                     SSVNREFRPFRDEESMSSVTRHRTRTLSVTSAGSALSCSTIPPELVKQKVKRQLTRQQ
                     KAAARRRLQKGEANVFTKQRRENMQNIKSSLEAASFWGD"
     exon            340..478
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            479..595
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            596..771
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            772..860
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1052
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1145
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1655
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1752
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..3197
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2249..2254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="hexamer: AATAAT"
     regulatory      2252..2257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="hexamer: AATAGA"
     polyA_site      2266
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="major polyA site"
     polyA_site      2271
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
     regulatory      3178..3183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3197
                     /gene="Riok2"
                     /gene_synonym="2010110K24Rik; 2410085M17Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtggcatact atgtaaaaca tttgcaactt gccaaatcag tcaaacgtaa attatttatt
       61 taggggctga aaaatcactt ctgcctgatt tttagctaca aaaacgagaa aaaaatagca
      121 tttttctact gcttcttgag cacgtgttct tacctagcga taagcacctt aaatacaggg
      181 cgcgtgcgcg ggcaggtcgc cgccgaaagc ctgcgagatt acccagcaac tttgcgtctt
      241 cccagagagc gtccgtggtg gaactgggca agcatgggga aggtgaatgt agccaagctt
      301 aggtacatga gccgcgatga cttcagagtt ctgactgcgg ttgaaatggg catgaaaaac
      361 catgagattg ttccctgcag tttgattgct tctatagcca gcctgaaaca tggtggctgt
      421 aataagattc taagagaatt ggtgaagcat aaacttatag cgtgggaacg tactaaaact
      481 gtgcagggct atcggttgac aaatgctggc tatgattacc tagctctgaa aacgctgtct
      541 tctagacagg tagttgaatc tgttggaaac cagatgggtg tgggcaaaga gtcagacatt
      601 tacattgttg ccaatgaagc cgggcagcag ctcgcactga agctccacag actcggaaga
      661 acttcctttc gaaatctgaa aaacaagcgc gattaccaca agcacaggca caatgtttct
      721 tggctttact tatctcggct ctcggccatg aaggaatttg cctatatgaa ggcattatat
      781 gagaggaaat ttccagttcc aaagccaatt gattataatc gccatgcagt gatcatggag
      841 cttattaatg gttatcccct atgtcagata catcatgttg aagaccctgc atcagtatat
      901 gatgaagcta tggaactgat agtcaaactg ggaaaccatg gactgattca tggagatttc
      961 aatgaattca atctcatgtt ggacaaagat gaccacatca ccatgattga ttttccgcag
     1021 atggtttcca catcccaccc aaatgctgaa tggtattttg acagagatgt taaatgcatt
     1081 cgagaattct tcatgaaacg ttttagctat gaaagtgagc tctacccaac cttcagtgat
     1141 atcaggaagg aggattctct tgatgttgag gtctctgcca gtggttacac aaaggaaatg
     1201 caagcagatg atgaactgct acatccagta ggcccagatg ataaaattac tgaaacagaa
     1261 gaggactctg acttcacgtt ttctgatgaa gaaatgttag aaaaagccaa agtgtggagg
     1321 tcagaattgg aaaaggaagc agacccagca gatgaatcag gtggatcctg gtgctgctcg
     1381 tctacagaca gcaaacaaat aaaggatggc ggcttaccag aggagagtgc tcatgtgtcc
     1441 agtttcgaag tgacggcatt aagccaagct gtagaagaga tggaaaggca ggttcttccc
     1501 cacaggtcgg ttactgagtt ctctgaggag agcaggagaa ctgaaaatga tgggcagcca
     1561 ggtcagagaa gccctgctgg ctctgaggac tgtgacgatg aaccccccca tctgattgcc
     1621 ttgtcatcag tgaacaggga gttcaggcct ttcagagacg aagagagtat gagtagtgtt
     1681 actcggcata gaacaagaac attgagtgtt acgtctgcgg gcagtgccct aagctgttca
     1741 acaatccctc cagaactggt gaagcagaag gtgaagcgtc agctgacaag gcagcagaag
     1801 gcggctgcca gacgccggct gcagaaagga gaagccaatg tgtttaccaa gcagcggagg
     1861 gaaaacatgc agaatattaa atcgagtttg gaagcagcca gcttttgggg agactaatag
     1921 atttctgata tgtattattt ttttagatgc tataatttta tttttagcca gtatattggt
     1981 cttttttgga ccaaagccag taaattatgg aaataaagcc tctttagact cagagcttcc
     2041 aaggtctgat taatagtaaa tctgccaagc actctgtttc ttaaaatttt tcaggtaact
     2101 aatttaaata taaaataacg tgtgaaaaaa acttcattgg cagtaattgc aaaacatgga
     2161 catcattttt atttcactgt gatgttcaga ctctgcctta aacaggtaga aaaatgactt
     2221 gcctcagatt ctggatttac atgtaataaa taatagaaca acttaatgca atctgtagtt
     2281 ctctcttttc tccttcagtc ggtcacactt gggcttttac tacattggtg agttggcttt
     2341 aggatcacct gagacagtca gtacacagtt atttgaccat tggagtgaaa ctgatcgtct
     2401 tctgtttctt tagacttctg gagtggagat gaatcagtaa tgggtagacg agcagcagcg
     2461 ggtcttttgg tgccgttctc attggccctg tggtcgtggg aaaaggaagt ttaggttcct
     2521 accagtattt ttgcagttgt cagctttccc caggtttcag cagaagtggg gggagggagg
     2581 gagggaggga gggagggagg gagagagaga gagagagaga gagagagaga gagagagaga
     2641 gagagagaga gaatgcatga ctccaggatg tgttcctacc tcagtgttaa caagtctcta
     2701 gaacaacact ctggtgtgat tcatccctca agcgcattct aattcagtca gtggaagtga
     2761 gtgtaagtat acaagcactg tcccttcagc ttccctgagc agatctcgaa tagaagatcg
     2821 tcttcagcaa gaagtgacca ccagatggca gtgtttgcca gcagtgacat gataaaaaca
     2881 ccagcttccc ttttacctta gcaaaattca ttctgactat ttgtaactga tcactatcta
     2941 agcgcattta aatgtttgga tatatgaata tgtcaagtgc agtttctttg aaaattagac
     3001 tgatttttgg aagtgttgat atagccctgt tttaaaatgt agtttaagct cagatactca
     3061 ttttttatac tctgctaaaa gtgtttctat aacataaact gatgttgaag atagtttaat
     3121 aagaccacag aatgtttatt ttgtaatacg tttttcttat gtccaaagca gattgggaat
     3181 aaaagtgttt gacctcaaaa a
//
