LOCUS       NM_053271               7278 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus regulating synaptic membrane exocytosis 2 (Rims2),
            transcript variant 3, mRNA.
ACCESSION   NM_053271
VERSION     NM_053271.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Emperador-Melero,J., Andersen,J.W., Metzbower,S.R., Levy,A.D.,
            Dharmasri,P.A., de Nola,G., Blanpied,T.A. and Kaeser,P.S.
  TITLE     Distinct active zone protein machineries mediate Ca2+ channel
            clustering and vesicle priming at hippocampal synapses
  JOURNAL   Nat Neurosci 27 (9), 1680-1694 (2024)
   PUBMED   39160372
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Rybiczka-Tesulov,M., Garritsen,O., Veno,M.T., Wieg,L., Dijk,R.V.,
            Rahimi,K., Gomes-Duarte,A., Wit,M., van de Haar,L.L., Michels,L.,
            van Kronenburg,N.C.H., van der Meer,C., Kjems,J., Vangoor,V.R. and
            Pasterkamp,R.J.
  TITLE     Circular RNAs regulate neuron size and migration of midbrain
            dopamine neurons during development
  JOURNAL   Nat Commun 15 (1), 6773 (2024)
   PUBMED   39117691
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7278)
  AUTHORS   Snyder,K., Dixon,C.E., Henchir,J., Gorse,K., Vagni,V.A.,
            Janesko-Feldman,K., Kochanek,P.M. and Jackson,T.C.
  TITLE     Gene knockout of RNA binding motif 5 in the brain alters RIMS2
            protein homeostasis in the cerebellum and Hippocampus and
            exacerbates behavioral deficits after a TBI in mice
  JOURNAL   Exp Neurol 374, 114690 (2024)
   PUBMED   38218585
  REMARK    GeneRIF: Gene knockout of RNA binding motif 5 in the brain alters
            RIMS2 protein homeostasis in the cerebellum and Hippocampus and
            exacerbates behavioral deficits after a TBI in mice.
REFERENCE   4  (bases 1 to 7278)
  AUTHORS   Wang,X., Xie,J., Tan,L., Lu,Y., Shen,N., Li,J., Hu,H., Li,H., Li,X.
            and Cheng,L.
  TITLE     N6-methyladenosine-modified circRIMS2 mediates synaptic and memory
            impairments by activating GluN2B ubiquitination in Alzheimer's
            disease
  JOURNAL   Transl Neurodegener 12 (1), 53 (2023)
   PUBMED   38012808
  REMARK    GeneRIF: N6-methyladenosine-modified circRIMS2 mediates synaptic
            and memory impairments by activating GluN2B ubiquitination in
            Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7278)
  AUTHORS   Young,T.R., Yamamoto,M., Kikuchi,S.S., Yoshida,A.C., Abe,T.,
            Inoue,K., Johansen,J.P., Benucci,A., Yoshimura,Y. and Shimogori,T.
  TITLE     Thalamocortical control of cell-type specificity drives circuits
            for processing whisker-related information in mouse barrel cortex
  JOURNAL   Nat Commun 14 (1), 6077 (2023)
   PUBMED   37770450
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7278)
  AUTHORS   Fukuda,M.
  TITLE     Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and
            Noc2. Identification of a critical determinant of Rab3A/Rab27A
            recognition by Rim2
  JOURNAL   J Biol Chem 278 (17), 15373-15380 (2003)
   PUBMED   12578829
REFERENCE   7  (bases 1 to 7278)
  AUTHORS   Fujimoto,K., Shibasaki,T., Yokoi,N., Kashima,Y., Matsumoto,M.,
            Sasaki,T., Tajima,N., Iwanaga,T. and Seino,S.
  TITLE     Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of
            cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis
  JOURNAL   J Biol Chem 277 (52), 50497-50502 (2002)
   PUBMED   12401793
REFERENCE   8  (bases 1 to 7278)
  AUTHORS   Kashima,Y., Miki,T., Shibasaki,T., Ozaki,N., Miyazaki,M., Yano,H.
            and Seino,S.
  TITLE     Critical role of cAMP-GEFII--Rim2 complex in incretin-potentiated
            insulin secretion
  JOURNAL   J Biol Chem 276 (49), 46046-46053 (2001)
   PUBMED   11598134
REFERENCE   9  (bases 1 to 7278)
  AUTHORS   Ozaki,N., Shibasaki,T., Kashima,Y., Miki,T., Takahashi,K., Ueno,H.,
            Sunaga,Y., Yano,H., Matsuura,Y., Iwanaga,T., Takai,Y. and Seino,S.
  TITLE     cAMP-GEFII is a direct target of cAMP in regulated exocytosis
  JOURNAL   Nat Cell Biol 2 (11), 805-811 (2000)
   PUBMED   11056535
REFERENCE   10 (bases 1 to 7278)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY242489.1, AB021131.1 and AK032619.1.
            
            On Jan 24, 2012 this sequence version replaced NM_053271.1.
            
            Transcript Variant: This variant (3) has multiple differences in
            the coding region but maintains the reading frame, compared to
            variant 1. It encodes isoform c which is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB021131.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                BY242489.1         2-47
            47-4846             AB021131.1         1-4800
            4847-7118           AK032619.1         1255-3526
            7119-7278           AK032619.1         3528-3687
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 15.45 cM"
     gene            1..7278
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /note="regulating synaptic membrane exocytosis 2"
                     /db_xref="GeneID:116838"
                     /db_xref="MGI:MGI:2152972"
     exon            1..253
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..4670
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /note="isoform c is encoded by transcript variant 3;
                     synaptic exocytosis regulator 2; Rab3 interacting protein
                     2; regulating synaptic membrane exocytosis protein 2; RIM
                     2; rab3-interacting protein 2; rab-3-interacting protein
                     2; synaptotagmin 3, related sequence; Rab3 interacting
                     protein 1"
                     /codon_start=1
                     /product="regulating synaptic membrane exocytosis protein
                     2 isoform c"
                     /protein_id="NP_444501.1"
                     /db_xref="CCDS:CCDS37067.1"
                     /db_xref="GeneID:116838"
                     /db_xref="MGI:MGI:2152972"
                     /translation="MSAPLGPRGRPAPTPAASQPPPQPEMPDLSHLTEEERKIILAVM
                     DRQKKEEEKEQSVLKIKEEHKAQPTQWFPFSGITELVNNVLQPQQKQPNEKEPQTKLH
                     QQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCG
                     GRVSLRSNKVMWVCNLCRKQQEILTKSGAWFYNSGSNTLQQPDQKVPRGLRNEEAPQE
                     KKAKLHEQPQFQGAPGDLSVPAVEKGRAHGLTRQDTIKNGSGVKHQIASDMPSDRKRS
                     PSVSRDQNRRYEQSEEREDYSQYVPSDGTMPRSPSDYADRRSQREPQFYEEPGHLNYR
                     DSNRRGHRHSKEYIVDDEDVESRDEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQ
                     MRIHAEVSRARHERRHSDVSLANAELEDSRISLLRMDRPSRQRSVSERRAAMENQRSY
                     SMERTREAQGQSSYPQRTSNHSPPTPRRSPIPLDRPDMRRADSLRKQHHLDPSSAVRK
                     TKREKMETMLRNDSLSSDQSESVRPPPPRPHKSKKGGKMRQVSLSSSEEELASTPEYT
                     SCDDVELESESVSEKGDSQKGKRKTSEQGVLSDSNTRSERQKKRMYYGGHSLEEDLEW
                     SEPQIKDSGVDTCSSTTLNEEHSHSDKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPR
                     DSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGAT
                     FEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISV
                     TSPMSPGMLRDVPQFLSGQLSIKLWFDKVGHQLIVTILGAKDLPSREDGRPRNPYVKI
                     YFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEE
                     SEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPRPSPYLPRRQLHGESPTRRLQR
                     SKRISDSEVSDYDCEDGVGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSPSGSPHRVD
                     VIGRTRSWSPSAPPPQRNVEQGHRGTRATGHYNTISRMDRHRVMDDHYSSDRDRSHPR
                     TGSVQTSPSSTPGTGRRGRQLPQLPPKGTLERSAMDIEERNRQMKLNKYKQVAGSDPR
                     LEQDYHSKYRSGWDPHRGADTVSTKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSER
                     PRGNRKISVFTSKMQNRQMGVSGKNLTKSTSISGDMCSLEKNDGSQSDTAVGALGTSG
                     KKRRSSIGAKMVAIVGLSRKSRSASQLSQTEGGGKKLRSTVQRSTETGLAVEMRNWMT
                     RQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMG
                     DIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARK
                     TLEPLYQQLLSFEESPQGRVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWF
                     KLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS"
     exon            254..286
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            287..373
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            374..584
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            585..895
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1821
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..2030
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2150
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2250
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2374
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2421
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2534
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2665
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2666..2837
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2991
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            2992..3060
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3061..3217
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3328
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3329..3438
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3539
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3540..3712
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3713..3954
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            3955..4096
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            4097..4209
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..4311
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            4312..4451
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     exon            4452..7278
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5287..5292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /note="hexamer: ATTAAA"
     polyA_site      5311
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /note="major polyA site"
     regulatory      5377..5382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
                     /note="hexamer: AATAAA"
     polyA_site      5401
                     /gene="Rims2"
                     /gene_synonym="2810036I15Rik; mKIAA0751; Rab3ip2; RIM2;
                     Rim2(+40A); Rim2(+44A); Rim2(+4A); Serg2; Syt3-rs"
ORIGIN      
        1 cagccgcccg cgcccgagcc gcggctgcca gcggagttgc ctccctgctt ccctagggtg
       61 gttcggctcc gccaaacatg tcggctccgc tcgggccccg gggccgcccg gctcccaccc
      121 cggcggcctc tcaacctcct ccgcagcccg agatgccgga cctcagccac ctcacggaag
      181 aggagaggaa aatcatcctg gctgtcatgg atcgtcagaa gaaagaagag gagaaggagc
      241 agtccgtgct caagatcaaa gaagaacaca aagcacaacc gacacagtgg tttcccttta
      301 gtgggatcac tgaactggta aataacgttc tgcagcccca gcaaaaacaa cccaatgaga
      361 aggagcccca gacaaagctg caccaacaat ttgaaatgta taaggagcaa gtcaagaaga
      421 tgggagagga atcgcagcag cagcaagagc agaagggtga tgccccgacc tgtggcatct
      481 gccacaagac aaaatttgca gatggatgcg gccataattg ttcctattgc caaaccaagt
      541 tctgtgctcg atgtggaggt cgagtgtctt tacgctcaaa caaggttatg tgggtgtgta
      601 atttgtgccg aaaacaacaa gaaatcctca ctaaatcagg agcatggttt tataatagtg
      661 ggtctaacac actgcagcaa cctgatcaaa aggttcctcg agggcttcga aatgaggaag
      721 cccctcagga gaagaaagca aaactacacg agcagcccca gttccaagga gccccaggtg
      781 acttatcagt acctgcagtt gagaaaggcc gagctcatgg gctcacaaga caggatacta
      841 ttaaaaatgg atcaggagtg aagcaccaga ttgccagtga catgccttca gacagaaaac
      901 gaagtccatc agtgtccagg gatcaaaatc gaagatacga gcaaagtgaa gaaagagagg
      961 actactcaca gtatgttcct tcagatggta caatgccaag atctccttcg gattatgctg
     1021 atagacgatc tcagcgtgag cctcaatttt atgaagaacc tggtcattta aattacaggg
     1081 attctaacag gagaggccat agacattcca aagagtatat tgtggatgat gaagatgtgg
     1141 agagcagaga tgaatatgaa agacaaagga gagaggagga ataccaggca cgctacagaa
     1201 gtgatccaaa tctggcccgg tatcccgtaa agccacaacc ctacgaagaa caaatgcgca
     1261 tccacgctga ggtgtccagg gcacgacatg agagaaggca cagtgatgtt tctttggcaa
     1321 acgctgaact agaagattcc aggatttctc tgctaaggat ggatagacca tcaaggcaaa
     1381 gatctgtatc tgaacgtaga gctgcaatgg aaaaccaacg atcgtattca atggaaagaa
     1441 ctcgagaggc tcagggacaa agttcttatc cacaaaggac ctcaaatcat agtcctccca
     1501 cccctcggcg gagccctata ccgcttgata gaccagacat gaggcgcgct gactccctac
     1561 ggaaacagca ccacttagat cccagctctg ctgtgaggaa aacgaagcga gaaaaaatgg
     1621 aaaccatgtt aaggaatgat tctttgagtt cagaccagtc cgagtcagtg aggccgcccc
     1681 caccaaggcc tcataaatcc aagaaaggag gtaaaatgcg ccaggtttca ctgagcagct
     1741 cggaggagga gctggcatcc acacctgagt atacaagctg tgatgatgtg gagctggaaa
     1801 gcgagagtgt gagtgagaaa ggggacagtc aaaagggaaa aagaaaaact agtgagcagg
     1861 gagttttgtc ggattctaac accaggtctg agagacaaaa gaaaaggatg tactatggtg
     1921 gccactcttt ggaagaggat ttggaatggt ctgagcctca gattaaggac tctggggtag
     1981 atacctgtag tagcacaacc cttaacgagg agcatagcca tagtgataag caccctgtga
     2041 cctggcagcc atccaaagat ggagatcgcc taattggtcg tattttatta aataagcgtt
     2101 taaaagatgg gagtgtacct cgagactcag gagcaatgct gggcttaaag gttgtaggag
     2161 gaaagatgac tgaatcaggt cgactttgtg catttattac caaagtaaaa aaaggaagtt
     2221 tagctgatac tgtaggacat cttagaccag gtgatgaagt cttggaatgg aatgggaggc
     2281 tattgcaagg agccacattt gaggaagttt acaacattat tctagaatcc aaacctgaac
     2341 cacaagttga gcttgttgtt tcaaggccaa ttggagatat tcctagaata cctgatagca
     2401 cgcatgcaca actggaatcc agttctagct catttgaatc tcaaaaaatg gaccgtcctt
     2461 ctatatccgt tacctcaccc atgagtcctg gcatgctgag ggatgtcccg cagttcttat
     2521 ctggacagct ttcaataaaa ctatggtttg acaaggttgg tcaccagttg atagttacaa
     2581 ttttgggagc aaaggatctc ccttccaggg aagatgggag gccaaggaat ccttatgtta
     2641 agatttactt ccttccagat agaagtgata aaaataagag aagaacaaaa acagtcaaga
     2701 aaactttgga acccaaatgg aaccagactt tcatttattc tcctgtccac cgaagagaat
     2761 tccgtgaacg aatgctggaa attacccttt gggatcaagc tagagttcga gaagaagaga
     2821 gcgaattctt aggagagatt ttaattgaat tggaaacagc tttgctagat gatgagccgc
     2881 actggtataa gctgcagacc catgatgtct cctcattgcc actccctcgc ccttccccat
     2941 atctgccccg gaggcagctc catggagaga gcccaacgcg caggctgcaa aggtcgaaga
     3001 gaataagtga cagtgaagtg tctgactacg actgcgagga tggcgtggga gtagtgtcag
     3061 attatcgaca caatggccgc gatcttcaaa gctccacgtt gtcggtgcca gaacaagtca
     3121 tgtcatcaaa tcattgctca ccatcagggt ctcctcatcg agtagatgtt ataggaagga
     3181 caaggtcatg gtcgcctagt gcccctcctc ctcaaaggaa tgtggaacag gggcaccgag
     3241 ggacacgtgc tactggccat tacaacacaa ttagccgaat ggatagacac cgtgtcatgg
     3301 atgaccacta ctcttcagat agagacaggt ctcaccctcg taccgggtct gtccagacaa
     3361 gcccatcaag tactccggga acaggacgaa ggggccgaca gcttccacag cttccaccaa
     3421 agggaacatt ggagagaagt gctatggata tagaggagag aaatcgccaa atgaaactta
     3481 acaaatacaa acaggtagcc ggatcagacc ccagactgga gcaagattac cattcgaagt
     3541 atcgctcagg atgggatcca catagagggg cagatactgt ttccactaaa tcctcggaca
     3601 gtgatgtaag tgatgtatct gcggtttcaa ggactagtag tgcttctcgt ttcagcagca
     3661 caagctacat gtccgtccaa tcagagcggc cgagaggaaa caggaaaatc agtgtcttta
     3721 catccaaaat gcaaaacaga cagatgggcg tgtcggggaa gaacttgacc aaaagcacca
     3781 gcatcagtgg agacatgtgc tcactggaga agaatgacgg cagccagtcc gacactgcag
     3841 tgggcgccct gggtaccagt ggcaagaagc ggcgatctag cattggggcc aaaatggtag
     3901 ctattgttgg tctctcacgg aaaagtcgca gtgcctctca actcagccaa accgaaggag
     3961 gaggtaaaaa gctacggagc actgttcaga gaagcacgga gaccgggcta gcagtggaga
     4021 tgaggaactg gatgacccgc caggccagcc gggaatccac agatggcagc atgaacagct
     4081 atagctcgga aggaaatctg atcttccctg gggtccgcct ggcctctgac agccagttca
     4141 gtgatttcct ggatggcctg ggccctgctc agctagtggg acgccagacc ctggctactc
     4201 ctgcaatggg tgacattcag gtgggaatga tggataaaaa gggacagctg gaggtagaaa
     4261 tcatccgggc gcgcggcctt gtggtaaaac caggttccaa gacactgcca gcaccgtatg
     4321 tcaaggtgta tctgttagac aacggagtct gcatagccaa aaagaaaacc aaggtggcga
     4381 gaaagaccct ggagcccctg taccagcagc tcttgtcctt cgaggagagc ccccagggga
     4441 gggtgttaca gatcattgtc tggggagatt atggtcgtat ggatcacaaa tcctttatgg
     4501 gagtggccca gatactctta gatgaactgg aactatccaa catggtgatt ggatggttca
     4561 aactcttccc tccttcctcc ctagtagatc caaccttggc acctctgaca agaagagctt
     4621 cccaatcgtc tctggaaagt tctaccggac cttcttactc tcgttcatag caactataaa
     4681 actgttgtca caacaaccag cgatacaaaa accagaagaa aacgcacagg tggaagcccc
     4741 tggtaacact gcatgcttga tgttgtgtct acagagccca cgtctaggga taccaagcag
     4801 tcctgtgttc tcagaggaag tcgtacacat tgtgccctag caaaggccct caggtgaaag
     4861 agcagagctg tgaagaacta tctatcaggt ttggagttca atgacactcg agttttggtc
     4921 cattctgaag ccatggatta atcttaaaga atcagtcagc ccttttcaat ggctccttta
     4981 tatttttatc aattttcttc atttatctga ccccaaagcc ctgttatgcc acagaaatgg
     5041 agctctacag ccattaagac acattacagg ccaaggagca aagacctagg aagcaagcat
     5101 caggtgaaaa gcaagagacc aaagaagtgg tcaggagcag agtcagccac cgttcctgga
     5161 ggggctccag cagggagagc ggccgccggg gctgctccgg gagggaggca ggcatgtcat
     5221 tcactaatgc cgagatgtac tggactctga aaatcaaaat tctgtgactg cactgtactg
     5281 aatgaaatta aagaaacttt ttttgcatgg acacagatta gctgaatatt taaattattt
     5341 tctcggggct gcaacttgca aaaaaaaaaa aaaaagaata aaaatcagcc attttcaaca
     5401 atttatatta tttttaaaaa taaatttcac tagtgcatgg ttttacaatg ggagagaatg
     5461 caacagggtg gtacaaagac acaccgtgct tattctttaa tcagagtctg tgttttggca
     5521 gacaatacaa atgaaaatac tttctagcag atacttaaaa ttctctttat gtgacaataa
     5581 gtataatata ttcaatttat ttccatgtta aatatacaga tcttatgatg ttcaatatgt
     5641 gcaaattttt cacatctttc ttcctcttgt ctgtctcact tttcttctcc cacttttaac
     5701 ctcttctttc ctttctcccc tcctcagtga gtcttactca acaccacaag tctttgaccc
     5761 gaccctctta tagcccagat ttgattcaga gccatagatg catctgaatt cacagatttc
     5821 tcagatctgt ccgagagctt tctttatttc aagcatgttg aaggattttg caacatgact
     5881 tgggacatac agcaagtcag cgcgcatttg acaaagaaga tatctgaaca gttgcagttt
     5941 attgtacagt gcatggtaat ttttttcacc ttaaagattc agtttacaag aaaattttca
     6001 aaccacatgg cattgtaatg tccaacaaat ttgtttttag caccagcatt agtcatacag
     6061 ggggttaagt attatttgta ggtcttaggt tttgtttgtt tgttaatttg gtaaagcagt
     6121 ttctttagct gatttccatt tactatgtga atagaaacac tgctcaacat gtggaaccct
     6181 gaaacacagg gctgtttatt aattcatttt tctgtaggaa aattcaattt ttcacaaatt
     6241 atatttctaa agaaatatag taaacataaa tttgcaacaa ttttaaagct ccaattttta
     6301 ggtgactcga ggaaaagtca atagttattt gatgccatca ccaaaaatct acatccctta
     6361 gtcaaaccca cctttggttt acaggcagtt cttaccactg ggtagcactg caaggtccaa
     6421 tttctgtcaa ggtccccaat caacaggaaa tgtcactggt caatgtaata aaccacccat
     6481 cccttctgtt tcttacgcac gctttgatta cacatccttg gagattgatt ttaacgtctt
     6541 gcaataattt ttgaaatcca tctacatccc atttgtttaa atggaggcca cacacacaca
     6601 cacacacaca cacacacaca tgttctttgt ttctcttttt attcaatgct gaccattgaa
     6661 aactgtcatg cttgatatgg tgcaaaagtt aaaatgagct atcactcaaa atgccttctc
     6721 ctaacgtggt gcaacatgaa ctacaccaag accgtgtctt gattttctga gacctaagat
     6781 ggatctagct gagggactga aaagaaggaa cccaatttaa ttgaaataat ttatacacct
     6841 gtgtaccagc aacaatttgg tttaatagac ctgtagtgtc tatgctcacc cttagagtag
     6901 aagattttcc tgacactaag atgcagtcac ataagacttt ctgcatattc ctgtacacat
     6961 tattcctcat tttaataaga aggacatgac tgtggaatat ttgtacatac ttgtcattac
     7021 tcagtattta tttaaaagtt ggtgtacttt tcttttttta attttcacat gtctgcacct
     7081 caacttgtgg ttaagtcatg gaaatagcac tgcagtgacc gctgctgccc tgtacataga
     7141 atgttttcaa gtaaagggaa ttccagttgg ggaagagtgg ggggaggaca gattagtcct
     7201 gtaacaaaca ccaactgatg taaatcaaat tcattctggt gatggtattt aacactttaa
     7261 ataaaacatt ttctttac
//
