LOCUS       NM_183172               5017 bp    mRNA    linear   ROD 15-DEC-2024
DEFINITION  Mus musculus RIC8 guanine nucleotide exchange factor B (Ric8b),
            transcript variant 2, mRNA.
ACCESSION   NM_183172
VERSION     NM_183172.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Tsisanova,E., Nobles,M., Sebastian,S., Ng,K.E., Thomas,A.,
            Weinstein,L.S., Munroe,P.B. and Tinker,A.
  TITLE     The ric-8b protein (resistance to inhibitors of cholinesterase 8b)
            is key to preserving contractile function in the adult heart
  JOURNAL   J Biol Chem 300 (7), 107470 (2024)
   PUBMED   38879012
  REMARK    GeneRIF: The ric-8b protein (resistance to inhibitors of
            cholinesterase 8b) is key to preserving contractile function in the
            adult heart.
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5017)
  AUTHORS   Papasergi-Scott,M.M., Kwarcinski,F.E., Yu,M., Panova,O.,
            Ovrutsky,A.M., Skiniotis,G. and Tall,G.G.
  TITLE     Structures of Ric-8B in complex with Galpha protein folding clients
            reveal isoform specificity mechanisms
  JOURNAL   Structure 31 (5), 553-564 (2023)
   PUBMED   36931277
REFERENCE   4  (bases 1 to 5017)
  AUTHORS   Sebastian,S., Nobles,M., Tsisanova,E., Ludwig,A., Munroe,P.B. and
            Tinker,A.
  TITLE     The role of resistance to inhibitors of cholinesterase 8b in the
            control of heart rate
  JOURNAL   Physiol Genomics 53 (4), 150-159 (2021)
   PUBMED   33719582
  REMARK    GeneRIF: The role of resistance to inhibitors of cholinesterase 8b
            in the control of heart rate.
REFERENCE   5  (bases 1 to 5017)
  AUTHORS   Nagai,M.H., Xavier,V.P.S., Gutiyama,L.M., Machado,C.F., Reis,A.H.,
            Donnard,E.R., Galante,P.A.F., Abreu,J.G., Festuccia,W.T. and
            Malnic,B.
  TITLE     Depletion of Ric-8B leads to reduced mTORC2 activity
  JOURNAL   PLoS Genet 16 (5), e1008255 (2020)
   PUBMED   32392211
  REMARK    GeneRIF: A crucial role for Ric-8B in development and novel
            insights into the signals that regulate mTORC2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5017)
  AUTHORS   Nagai,Y., Nishimura,A., Tago,K., Mizuno,N. and Itoh,H.
  TITLE     Ric-8B stabilizes the alpha subunit of stimulatory G protein by
            inhibiting its ubiquitination
  JOURNAL   J Biol Chem 285 (15), 11114-11120 (2010)
   PUBMED   20133939
  REMARK    GeneRIF: Ric-8B plays a critical and specific role in the control
            of G alpha(s) protein levels by modulating G alpha(s)
            ubiquitination and positively regulates G(s) signaling
REFERENCE   7  (bases 1 to 5017)
  AUTHORS   Kerr,D.S., Von Dannecker,L.E., Davalos,M., Michaloski,J.S. and
            Malnic,B.
  TITLE     Ric-8B interacts with G alpha olf and G gamma 13 and co-localizes
            with G alpha olf, G beta 1 and G gamma 13 in the cilia of olfactory
            sensory neurons
  JOURNAL   Mol Cell Neurosci 38 (3), 341-348 (2008)
   PUBMED   18462949
  REMARK    GeneRIF: Ric-8B interacts with two different olfactory G protein
            subunits and is implicated as an important protein in odorant
            signal transduction.
REFERENCE   8  (bases 1 to 5017)
  AUTHORS   Von Dannecker,L.E., Mercadante,A.F. and Malnic,B.
  TITLE     Ric-8B promotes functional expression of odorant receptors
  JOURNAL   Proc Natl Acad Sci U S A 103 (24), 9310-9314 (2006)
   PUBMED   16754875
  REMARK    GeneRIF: Results show that Ric-8B, a putative guanine nucleotide
            exchange factor for Galphaolf, promotes efficient heterologous
            expression of odorant receptors.
REFERENCE   9  (bases 1 to 5017)
  AUTHORS   Von Dannecker,L.E., Mercadante,A.F. and Malnic,B.
  TITLE     Ric-8B, an olfactory putative GTP exchange factor, amplifies signal
            transduction through the olfactory-specific G-protein Galphaolf
  JOURNAL   J Neurosci 25 (15), 3793-3800 (2005)
   PUBMED   15829631
REFERENCE   10 (bases 1 to 5017)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY272676.1, BC051080.1 and BC040762.1.
            
            On Oct 13, 2012 this sequence version replaced NM_183172.1.
            
            Transcript Variant: This variant (2) contains an alternate in-frame
            exon in the coding region compared to variant 1. It encodes isoform
            2, which is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.274670.1,
                                           SRR1660815.101227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 BY272676.1         2-4
            4-3072              BC051080.1         1-3069
            3073-5017           BC040762.1         1627-3571
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 41.63 cM"
     gene            1..5017
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /note="RIC8 guanine nucleotide exchange factor B"
                     /db_xref="GeneID:237422"
                     /db_xref="MGI:MGI:2682307"
     exon            1..213
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1812
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /note="isoform 2 is encoded by transcript variant 2;
                     resistance to inhibitors of cholinesterase 8B;
                     synembryn-B; resistance to inhibitors of cholinesterase 8
                     homolog B; chaperone Ric-8B"
                     /codon_start=1
                     /product="chaperone Ric-8B isoform 2"
                     /protein_id="NP_898995.1"
                     /db_xref="CCDS:CCDS24085.1"
                     /db_xref="GeneID:237422"
                     /db_xref="MGI:MGI:2682307"
                     /translation="MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRK
                     KLCEGIFKVLVKEVPTTCQVSCLEVLRILSRDKKILVPVTTKENMQILLRLAKLHESD
                     DSLEKVSEFPVIVESLKCLCNIVFNSQMAQQLSLELNLAAKLCNLLRKCKDRKFINDI
                     KCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHNGPP
                     LSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEE
                     LHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPSDKTTEKDTALKNSTMVY
                     NGMNMEAIHVLLNFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPL
                     RDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAG
                     LLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQK
                     EYEAMKLVNMLDKLSREELLKPMGLKPDGTITPLEEALSQYSVIEETSSDTD"
     exon            214..261
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            262..870
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            871..965
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1194
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1290
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1435
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1580
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1700
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..4962
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3045..3050
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /note="major polyA site"
     regulatory      4942..4947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
                     /note="hexamer: AATAAA"
     polyA_site      4962
                     /gene="Ric8b"
                     /gene_synonym="Ric-8; Ric-8b"
ORIGIN      
        1 ggagggaggg agggaaggag ggaaggaagg aagctgggaa ggagcgaggt agcgggggcg
       61 cgaggcgttt acctgggagg cagcggctgg ggcgcgcaca gcggctgcgg ctcccccgca
      121 cctgcggcca tggatgaaga gcgcgccctc tacatcgtcc gggccggcga ggcgggggct
      181 atcgagcggg tcctgaggga ttacagcgac aagcataggg ctactttcaa atttgaatca
      241 gcagatgaag ataaaagaaa gaaactctgt gaaggcatat ttaaggtcct tgtcaaggag
      301 gtcccaacga catgccaagt gtcctgcctg gaagtactcc gcattctctc cagagacaaa
      361 aagatcttag ttcctgtaac aactaaggaa aatatgcaaa tactgctgag actagccaag
      421 ctccacgagt cagacgattc tttggagaag gtgtcagagt tcccagttat tgtggaatca
      481 ttgaagtgtc tgtgtaacat cgtgttcaac agtcagatgg cacagcagct tagcctggaa
      541 ctgaatctcg ctgcaaagct ctgtaacctc ctaaggaagt gcaaggaccg aaaatttatc
      601 aatgacataa agtgctttga cttgcgcttg ctcttcgtct tgtcactttt gcacactgac
      661 atcaggtcac aattgcgcta tgagctccag ggactcccgc tgctaaccca gatcttggaa
      721 agtgccttta gcatcaagtg gaccgatgag tatgagtcgg ccatagacca taatggacct
      781 cctctctcac ctcaggagac agactgtgcc attgaggccc tcaaagctct gttcaatgtg
      841 acggtagaca gttggaaggt gcataaagag agcgattctc atcagtttcg tgtcatggca
      901 gctgtccttc gccattgttt actaattgta ggtccaactg aagacaaaac cgaagagctg
      961 cacagtaatg ccgtcaacct tttaagcaat gttccagtct cttgtttgga tgttctcatt
     1021 tgtccattaa cccatgaaga aacagcccaa gaggcagcaa ctctagatga actgcccagt
     1081 gataaaacaa cggagaaaga cacagctttg aaaaacagta ccatggtata caatggcatg
     1141 aatatggagg ccattcacgt tttactcaat tttatggaga agagaattga taagggaagc
     1201 agctatagag agggtctaac tccagttctc agcttattaa cagaatgttc ccgagcccat
     1261 cggaacatcc gaaaatttct caaagatcag gttttaccgc ctttgagaga tgtgacaaat
     1321 cgaccagaag tcggctcaac cgtgagaaat aagctggttc gtctcatgac acatgttgac
     1381 ctcggagtca agcagattgc tgctgagttc ctttttgtcc tttgcaaaga gagagtggat
     1441 agcctgctga aatacactgg ctatgggaac gctgcaggac tcttggcggc caggggcctc
     1501 ttggctggag gaagaggaga taattggtac tcagaggacg aggacacgga cactgaagaa
     1561 tacaaaaacg caaaaccaaa cattaatctt atcactggtc atttagagga accaatgcca
     1621 aaccctatag atgaaatgac agaagaacaa aaagaatatg aagccatgaa acttgtcaac
     1681 atgcttgata aactttccag agaggagttg cttaaaccaa tgggactaaa acctgacggg
     1741 acaataacgc ctttggagga agcactcagc cagtattctg tcatcgaaga gaccagctcg
     1801 gacacagact gaaagcatca tcacctgctc cactctcaat atggctttta tcagcatctt
     1861 ttctctgtag ctccagggga atctttttct ctaaaacgtt tatgccctcg ctttggctag
     1921 aaacacatta actggtttac tcattgggaa tccagcatat ttaagaggta atcctgtgtt
     1981 ttctgcaaca ttgaggcatt catacagagc tgcagttagg cagagttgcg gggctagaag
     2041 cagaagcaga catcccattg caccaggaca gagtacggga cttcactcgg caagcagccc
     2101 tggtcagatc tggcagtctt gttgccgaag attctgcgtg tccttccacc cacccatgtc
     2161 aactgcccct tctgagtctc tccagcgacc tggggcccct gagggcccga ggagctgata
     2221 aataagggct gaggcaagtg actccttaga tcagcactga ctgaacgaaa gccaggcagc
     2281 tgttctgtaa gcctagccct acccttcatt tcagttttat acaaatgtgg aaacacacac
     2341 acacgcacgc acacacacac atggccccag acttacaaac ctattacact ctaaaagtgt
     2401 gtcatttagc gaaaacttga gattacattt cttcctggga ggagttagaa gtaatggttt
     2461 agttcaggca gctacacgtg cctatttatc cctcgttcct gaacactact agtaccgtag
     2521 ggctggacca ccatctgtac cgttgcgtcc acagtgtgca ttctgaggta tcactcaggg
     2581 tgccaagtac acactcatcc tccaggcaac agagaggatg gggctccccg agtagtggga
     2641 gttcttttgt ggcctgtgct gggggtaggg agccaacagc acccgtttgt acataaaggt
     2701 cattcgtatg tcacatgttt taaactgggg tctctgtgtg tatttgtgtg tgtgttctac
     2761 ctttctacca tatgtcatca gtttaatggt aactttattt atttaaaaac aaagaaacac
     2821 aaatagttac tattaaactg attaaggctg tttattttta ctcctagaaa tggtcatatg
     2881 aagtagatgt gcaatcatgt agacagcagg ccaaggtgat cagtggctaa agttgaaaag
     2941 gggttggttt ctttcatata tgtatgtgtg tgtacatgcg catacataga tatatgcaca
     3001 catagattat gtgcttgacc actgcctttt aaaactttaa attaaataaa acattgggga
     3061 tgattcaatt tagccatctg tgtgtgtgtt tctttatgga tacgtggtct aaggagacta
     3121 ttgcacagct tatgttttaa agtctttctc cacctgctcc ctcacctgtc attgtgcagc
     3181 ctctttccag gacagagtgt gcatagatag cattggtgag aattcgttta gcttcaatgg
     3241 tccctggcct ttgagctacc agacagatgc caggaagttc ttaaaattta ctctgaaggg
     3301 aacccttaaa gagtagtcac catcactaag atttatattc atttcaaaca tgtttggtca
     3361 cttggacttt gagccctcac cccacacaaa ggcagagaag gttagtgtgt ttcaagcagg
     3421 caccctaaaa acagtaaaag gcgtcagccg ggagtgggga gccttcagtg cccagattcc
     3481 tcctccagat ccaaatgggg gccagtgtgc acagcaaggt gagcaggaag aaacaccatc
     3541 caaggctcag cagatagtgt gtgtgacagg tggccagaga cagctttcct aatagtggcc
     3601 ctgagcggct ctaaaaggat caaccaaaag acttgtaaga gactcgggaa tgtccgtaca
     3661 tcaaggactc tcatccataa ctattgtttt agccaatggc accacccaat aagattttca
     3721 ttttattctg ccgttcataa ttttagttaa gtagaaaaaa attcagagga gcaggaactt
     3781 agttattttg ttacttcatt ctacaaattc atttcaaaag tatttttata atctctggtc
     3841 agatagcttt tccatgagca tcagcctacg aaagcatgct gtgcccggtg aaacgccacc
     3901 ttgccgcctc tgcgcaagtg cttgcacagt gccgtagctg tgctccgacg ctttcaacaa
     3961 gttcctttca ccatgcatct cttgcatcta ggcacttaat tttttttttt aaggagtaat
     4021 tctttaaaag atggagtagc ttcaccatgc acacagagag cactgagcag cgagcttcat
     4081 gtcttctttg taacctcacc ccatctccaa gcagagcctt tccagagagc tgcttaactt
     4141 cctagaccct cagcaagcca gggtccagct gtgggcttga cacctgcacc ttccctgttg
     4201 catgtaggac cactagccag aggctttaag acagcagaca ggctgcggaa ggtggtcctt
     4261 ctcagaccat tttaagcaat aactatttga gcagcagtct ctggtgttcc catcagggag
     4321 gtacctgcac gagcgggaga agcttggtca gttagcagat tttaggtgaa gacctggaca
     4381 cccttaggtc agagagcaac agtgtgggtc ggtcctccag tgagaggggt cagccagtga
     4441 ccacggccgt ttacatacac tggacttggg aatgcacgcc ctaaaacaag gcattggaac
     4501 tcctcagagg aacagtttcc agatgtctgt ggcccgtttg catgtccagt ggtttttagt
     4561 ggcagtcagg gcaaatgctg tcctttctct ataaatgaca ggttgaatga agctttgcaa
     4621 atggctggac agcaccctgg cacgtgagtc ttgctgcccc caggactagg tatcactctc
     4681 cttcagcttt ctttggcccc accttgtcta tcagagtcat acatgcttct tgtagaatgt
     4741 ttagaaagtg cggaaacagt aaaactagct attgacccag agccctccca cccccgagag
     4801 catcactgtt catgtttggt gtattccttt cctatatttt ttctatgcat atataattta
     4861 ttttactaca agattgggat catgctgtgt ttacagattt gtaccctgct ttccttaaca
     4921 ttgtgagcat tttcccatac caataaatag tcttcaaatg ttaaaaaaaa aaaaaaaaaa
     4981 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
