LOCUS       NM_027897               3439 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus rhophilin, Rho GTPase binding protein 2 (Rhpn2), mRNA.
ACCESSION   NM_027897
VERSION     NM_027897.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Liu,Z., Fang,F., Li,J., Zhao,G., Zang,Q., Zhang,F. and Die,J.
  TITLE     [RHPN2 is highly expressed in osteosarcoma cells to promote cell
            proliferation and migration and inhibit apoptosis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 42 (9), 1367-1373 (2022)
   PUBMED   36210710
  REMARK    GeneRIF: [RHPN2 is highly expressed in osteosarcoma cells to
            promote cell proliferation and migration and inhibit apoptosis].
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Yorgan,T.A., Peters,S., Amling,M. and Schinke,T.
  TITLE     Osteoblast-specific expression of Panx3 is dispensable for
            postnatal bone remodeling
  JOURNAL   Bone 127, 155-163 (2019)
   PUBMED   31202927
REFERENCE   3  (bases 1 to 3439)
  AUTHORS   Lal,M.A., Andersson,A.C., Katayama,K., Xiao,Z., Nukui,M.,
            Hultenby,K., Wernerson,A. and Tryggvason,K.
  TITLE     Rhophilin-1 is a key regulator of the podocyte cytoskeleton and is
            essential for glomerular filtration
  JOURNAL   J Am Soc Nephrol 26 (3), 647-662 (2015)
   PUBMED   25071083
REFERENCE   4  (bases 1 to 3439)
  AUTHORS   Sewell,W., Sparrow,D.B., Smith,A.J., Gonzalez,D.M., Rappaport,E.F.,
            Dunwoodie,S.L. and Kusumi,K.
  TITLE     Cyclical expression of the Notch/Wnt regulator Nrarp requires
            modulation by Dll3 in somitogenesis
  JOURNAL   Dev Biol 329 (2), 400-409 (2009)
   PUBMED   19268448
REFERENCE   5  (bases 1 to 3439)
  AUTHORS   Behrends,J., Clement,S., Pajak,B., Pohl,V., Maenhaut,C.,
            Dumont,J.E. and Schurmans,S.
  TITLE     Normal thyroid structure and function in rhophilin 2-deficient mice
  JOURNAL   Mol Cell Biol 25 (7), 2846-2852 (2005)
   PUBMED   15767687
  REMARK    GeneRIF: Mice lacking rhophilin 2 develop normally, have normal
            life spans, and are fertile; rhophilin 2 does not play an essential
            role in thyroid physiology
REFERENCE   6  (bases 1 to 3439)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ080543.1, AK004849.1 and AC154126.2.
            
            On May 1, 2009 this sequence version replaced NM_027897.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050214.1, SRR9219382.205139.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                CJ080543.1         1-77
            78-2449             AK004849.1         1-2372
            2450-3439           AC154126.2         22406-23395         c
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 21.36 cM"
     gene            1..3439
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /note="rhophilin, Rho GTPase binding protein 2"
                     /db_xref="GeneID:52428"
                     /db_xref="MGI:MGI:1289234"
     exon            1..175
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..2167
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /note="GTP-Rho-binding protein 2"
                     /codon_start=1
                     /product="rhophilin-2"
                     /protein_id="NP_082173.3"
                     /db_xref="CCDS:CCDS21148.1"
                     /db_xref="GeneID:52428"
                     /db_xref="MGI:MGI:1289234"
                     /translation="MTDTLLPAAPQPLEKEGDDYFRKGCNPLAQTGRSKLQNQRAALN
                     QQILKAVRMRTGAENLLKVATNQKVREQVRLELSFVNSDLQMLKEELEGLNISVGVYQ
                     GTEEAFTIPLIPLGLKETKEVDFSIVFKDFILEHYSEDSYLYEDDIADLMDLRQACRT
                     PSRDEAGVELLMSYFIQLGFVESRFFPPTRHMGLLFTWYDSFTGVPVSQQTLLLEKAS
                     VLFNIGALYTQIGTRCNRQTQAGLESAVDAFQRAAGVLNYLKETFTHTPSYDMSPAML
                     SVLVKMMLAQAQESVFEKVCLPGIQNEFFVLVKVAQEAAKVAEAYRQLHAAMSQEPVK
                     ENIPYSWASVAYVKAYHYGALAHYFAATLLIDHQLKPGADEDHQEKCLSQLYDRMPEG
                     MTPLATLKNAGQRVLLGKGHLHRAIGFHEESLREANLCKKLRDIQVLRDVLSAAHQRT
                     QLKHTQHREDDDLLNLIDAPDVLPKTEREVKITFPDFSKVTVTDFFQKLGPLSVFSAS
                     KRWSPPRGIHFTVEEGDLGFTLRGNTPVQVHFLDPHCSASLAGAKEGDYIVSIQGVDC
                     KWLTVSEVMKLLKSFGGEEVEMKVVSLLDSTSSMHNKCATYSVGMQKTYSMICLSMDD
                     DDKADKTKKISKKLSFLSWGTSKNRQKSASTLCLPEVGLARSQNKKKLPTPFSLLNSD
                     SSLY"
     exon            176..291
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            292..420
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            421..496
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            497..574
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            575..699
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            700..866
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1054
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1211
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1331
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1526
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1603
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1750
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1906
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..3439
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3411..3416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /note="hexamer: AATAAA"
     polyA_site      3439
                     /gene="Rhpn2"
                     /gene_synonym="1300002E07Rik; D7Ertd784e"
                     /note="major polyA site"
ORIGIN      
        1 agggcggctg gctggtcggg gcgggagcgg gggcgggtcc cggcgcctcc tggggccctt
       61 agtagccact gcacctcagt tcaccctgga ctcccgcgct gccagcatga ccgatacgct
      121 gctacccgcg gctccccagc cgctggagaa agagggtgac gactactttc gaaagggttg
      181 taatcccctt gcacaaactg gccggagcaa gctgcagaat caaagggctg ccttgaacca
      241 gcagatcctg aaggctgtgc ggatgcggac aggagccgag aaccttctga aggtcgccac
      301 aaaccagaag gtgcgggaac aggttcgcct ggagctgagc tttgtcaact cagacctgca
      361 gatgctgaag gaggagctgg aggggctcaa catctccgtg ggagtgtacc agggcaccga
      421 ggaagcgttc accattcctc tgattcctct cggcctgaag gagaccaaag aagttgactt
      481 ttccatcgtc ttcaaggatt ttattttgga acactacagt gaagatagct atttgtatga
      541 ggatgacatt gcagacctta tggacctgcg acaggcctgt cgaacaccca gcagggatga
      601 ggcgggggtc gaactgctca tgtcgtactt cattcagctg ggctttgtgg agagcaggtt
      661 cttcccaccc acgcgtcaca tggggctcct gtttacctgg tatgactcct tcactggggt
      721 tccggtcagc cagcagactt tgctgttgga gaaggccagc gttctcttca acattggggc
      781 gctctacacc cagatcggga cccgctgtaa ccggcagacg caggctggct tagagagcgc
      841 tgtggatgcc tttcaaagag ctgcaggggt tttaaactac ctgaaagaga ccttcactca
      901 cactccaagt tacgacatga gccctgccat gctcagtgtg ctggtcaaaa tgatgctcgc
      961 ccaagcccaa gaaagtgtgt ttgagaaagt ctgcctcccg gggatccaga acgagttctt
     1021 cgtgctggta aaggtggctc aggaggctgc caaggtggca gaggcctacc ggcagctgca
     1081 tgcggccatg agccaggaac cagtgaaaga gaacatcccg tattcgtggg ccagtgtggc
     1141 ctatgtgaag gcctaccact atggggccct ggctcactac tttgcggcca ccctcctcat
     1201 cgaccaccag ctaaagccag gcgcagacga ggaccaccaa gagaagtgtc tgtcccagct
     1261 ctatgaccgc atgccagaag ggatgacgcc gctggccacg ctgaagaatg ctgggcagcg
     1321 tgtgctgctg ggtaaaggac atctgcaccg ggccattggt tttcacgagg agtcactgag
     1381 ggaggccaac ctgtgcaaga agctgcgtga cattcaggtg ctccgggatg tgctgtctgc
     1441 tgcccatcag cgcactcagc tcaagcacac ccagcaccga gaggatgacg accttctgaa
     1501 cctgattgac gctccagatg tccttccaaa aactgagcga gaggtcaaaa taaccttccc
     1561 cgacttttcc aaagtgacag tgacagactt cttccagaag ctgggtcccc tgtctgtgtt
     1621 ttcggccagc aagagatggt cacctcctcg gggcattcac ttcacagtgg aggaagggga
     1681 cttggggttc accttacgag ggaacacccc agtccaggta cacttcctgg atcctcactg
     1741 ctctgcttcg cttgcagggg ccaaggaagg agattacatt gtttccattc aaggcgtgga
     1801 ttgcaagtgg ctgacagtga gtgaggttat gaagttgctg aagagctttg ggggagaaga
     1861 ggttgagatg aaggtcgtga gcctcctgga ctccacatcg tccatgcata ataagtgtgc
     1921 cacttactct gtggggatgc agaaaactta ctccatgata tgcctgtcca tggacgatga
     1981 tgacaaagct gacaaaacca agaagatctc aaaaaagctc tccttcttga gttggggcac
     2041 tagtaaaaac agacagaagt cggccagcac gctgtgcctc cccgaggttg gcctggcgag
     2101 gtcgcaaaac aagaagaaac tcccgactcc cttcagcctg ctcaactctg acagctctct
     2161 gtactgaagg gaggagccag aacaggccac accgaggtcg ccacagctgc ctaaccgccc
     2221 atgccgcgat gggactgttc tccaacccca tcttctcaga ctgtaaactt tgatgtgctt
     2281 gtgaacatga aaaagagacc tctagaaatt ggtgagaaac ccagccgcat ggggattttc
     2341 acattgctgc agttatttat tctataatgt agggcataga agagcctgaa tattttagct
     2401 gttttttttg gttttccaag acaattttct gtgtagccct gcctgcctta gaagttgccc
     2461 tggtgaccag gctggcctcg aactttgaga tccacctgcc tctgcctccc aagtgctggg
     2521 attaaggtgt gtgccaccac cacccagcag ctgctattac tgtttttctt ggctgtgatt
     2581 atgatattca ggcaccgtga gagatgaatt gggaccacct actttggtac tatatccgag
     2641 tgtttagatt tgtatagtag tgatttctta agttcaagtg actcttgagt ggaggcaggt
     2701 tagaggtgtt aactaaaacc aggctgcttg gaagatttct gtgagtttga ggccagcctg
     2761 gtcgacatag tgagttccag gacagctggg gctacacaga gaaaccctct ctcaaacaaa
     2821 caaaaccgaa acaggctgct ttaagtactt gggaattcat gagatgaaat gacacatctt
     2881 ctgtcatccc tgcataattt gactgttctt tcccagatga agtctagtgc caattttgtc
     2941 tttccttcct ccagaatcag cccccctcag ctatatcata ctgaccagta gctaacacag
     3001 tggcagtgtt aggagatacc gtctacaggg tagataatat gctgtttgat actcaaaacg
     3061 ttgctctttt tgtttaaagt aggaggtatg taactgtgta ttttaagagt cttggtttaa
     3121 gtagtagttt cgcagtttac aaggtaaaga cgtaaactta ctcaagttga aacctctact
     3181 cgactttgta agatgtatct accaacttga ctttggggct ataggttgga tgacactgtg
     3241 acagagtcac ggttgacaca gttcaagtga taagtttaag gatatatagc ctgtggatct
     3301 taaagtaaca aggatttaaa tatttttcta ttgtgcaata tctggagaac acaaatgcct
     3361 taaggaaagt gtgcccgcta tccctggggc tctgtttgta taaatattta aataaagtca
     3421 tgttcattgt ttgagttaa
//
