LOCUS       NM_001411251            2258 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus rhophilin, Rho GTPase binding protein 1 (Rhpn1),
            transcript variant 3, mRNA.
ACCESSION   NM_001411251 XM_006520447
VERSION     NM_001411251.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Nilsson,D., Heglind,M., Arani,Z. and Enerback,S.
  TITLE     Foxc2 is essential for podocyte function
  JOURNAL   Physiol Rep 7 (9), e14083 (2019)
   PUBMED   31062503
REFERENCE   3  (bases 1 to 2258)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2258)
  AUTHORS   Lal,M.A., Andersson,A.C., Katayama,K., Xiao,Z., Nukui,M.,
            Hultenby,K., Wernerson,A. and Tryggvason,K.
  TITLE     Rhophilin-1 is a key regulator of the podocyte cytoskeleton and is
            essential for glomerular filtration
  JOURNAL   J Am Soc Nephrol 26 (3), 647-662 (2015)
   PUBMED   25071083
  REMARK    GeneRIF: essential for the integrity of the glomerular filtration
            barrier and a key determinant of podocyte cytoskeleton architecture
REFERENCE   5  (bases 1 to 2258)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 2258)
  AUTHORS   Stylianou,I.M., Christians,J.K., Keightley,P.D., Bunger,L.,
            Clinton,M., Bulfield,G. and Horvat,S.
  TITLE     Genetic complexity of an obesity QTL (Fob3) revealed by detailed
            genetic mapping
  JOURNAL   Mamm Genome 15 (6), 472-481 (2004)
   PUBMED   15181539
REFERENCE   7  (bases 1 to 2258)
  AUTHORS   Peck,J.W., Oberst,M., Bouker,K.B., Bowden,E. and Burbelo,P.D.
  TITLE     The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton
            organization
  JOURNAL   J Biol Chem 277 (46), 43924-43932 (2002)
   PUBMED   12221077
REFERENCE   8  (bases 1 to 2258)
  AUTHORS   Fujita,A., Nakamura,K., Kato,T., Watanabe,N., Ishizaki,T.,
            Kimura,K., Mizoguchi,A. and Narumiya,S.
  TITLE     Ropporin, a sperm-specific binding protein of rhophilin, that is
            localized in the fibrous sheath of sperm flagella
  JOURNAL   J Cell Sci 113 (Pt 1), 103-112 (2000)
   PUBMED   10591629
REFERENCE   9  (bases 1 to 2258)
  AUTHORS   Nakamura,K., Fujita,A., Murata,T., Watanabe,G., Mori,C., Fujita,J.,
            Watanabe,N., Ishizaki,T., Yoshida,O. and Narumiya,S.
  TITLE     Rhophilin, a small GTPase Rho-binding protein, is abundantly
            expressed in the mouse testis and localized in the principal piece
            of the sperm tail
  JOURNAL   FEBS Lett 445 (1), 9-13 (1999)
   PUBMED   10069364
REFERENCE   10 (bases 1 to 2258)
  AUTHORS   Watanabe,G., Saito,Y., Madaule,P., Ishizaki,T., Fujisawa,K.,
            Morii,N., Mukai,H., Ono,Y., Kakizuka,A. and Narumiya,S.
  TITLE     Protein kinase N (PKN) and PKN-related protein rhophilin as targets
            of small GTPase Rho
  JOURNAL   Science 271 (5249), 645-648 (1996)
   PUBMED   8571126
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116393.10.
            
            On Aug 23, 2022 this sequence version replaced XM_006520447.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784651.1274055.1,
                                           SRR17784647.939383.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-167               AC116393.10        84254-84420
            168-283             AC116393.10        88117-88232
            284-412             AC116393.10        88935-89063
            413-488             AC116393.10        89183-89258
            489-566             AC116393.10        89960-90037
            567-691             AC116393.10        90401-90525
            692-858             AC116393.10        90612-90778
            859-1052            AC116393.10        90934-91127
            1053-1209           AC116393.10        91500-91656
            1210-1320           AC116393.10        91734-91844
            1321-1515           AC116393.10        92203-92397
            1516-1592           AC116393.10        92948-93024
            1593-1739           AC116393.10        93165-93311
            1740-1901           AC116393.10        93396-93557
            1902-2258           AC116393.10        93998-94354
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 34.76 cM"
     gene            1..2258
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /note="rhophilin, Rho GTPase binding protein 1"
                     /db_xref="GeneID:14787"
                     /db_xref="MGI:MGI:1098783"
     exon            1..167
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2075
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /note="isoform 3 is encoded by transcript variant 3;
                     rhophilin-1; GTP-Rho-binding protein 1; GTP-rho binding
                     protein 1"
                     /codon_start=1
                     /product="rhophilin-1 isoform 3"
                     /protein_id="NP_001398180.1"
                     /db_xref="GeneID:14787"
                     /db_xref="MGI:MGI:1098783"
                     /translation="MILEERPDGQGTGEESSRPQDDGSIRKGYGSFVQNQPGQLQSHR
                     ARLHQQISKELRMRTGAENLYRATSNTWVRETVALELSYVNSNLQLLKEELAELSTSV
                     DVDQPEGEGITIPMIPLGLKETKELDWATPLKELISEHFGEDGTSFETEIQELEDLRQ
                     ATRTPSRDEAGLDLLAAYYSQLCFLDARFFSPSRSPGLLFHWYDSLTGVPAQQRALAF
                     EKGSVLFNIGALHTQIGARQDCSCTEGTNHAAEAFQRAAGAFRLLRENFSHAPSPDMS
                     AASLSMLEQLMIAQAQECIFKGLLLPASATPDICPDQLQLAQEAAQVATEYGLVHRAM
                     AQPPVRDYLPASWTNLAHVKAEHFCALAHYHAAMALCESHPAAKGELARQEHVFQPST
                     PHEPLGPTLPQHPEDRRKLAKAHLKRAILGQEEALRLHTLCRVLRKVDLLQVVVTQAL
                     RRSLAKYSQLEREDDFFEATEAPDIQPKTHQTPEVRMPSLSQVKVTDFFHRLGPLSVF
                     STKNRWQLVGPVHMTRGEGSFGFTLRGDSPVLIAAVVPGGQAESAGLKEGDYIVSVNG
                     QPCKWWKHLEVVTQLRSMGEEGVSLQVVSLLPSPEPRGTGPRRAALLWNQRECGFETP
                     MPTRTRPWPILGWSRKNKQGKTGSHPDPCTNRNCVTCP"
     exon            168..283
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            284..412
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            413..488
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            489..566
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            567..691
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            692..858
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1052
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1209
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1320
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..1515
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1592
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1739
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1901
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2258
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2237..2242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /note="hexamer: ATTAAA"
     polyA_site      2258
                     /gene="Rhpn1"
                     /gene_synonym="Grbp; mKIAA1929; Rhophilin"
                     /note="major polyA site"
ORIGIN      
        1 agtcggagca gcgcggctga tcctccctgg agcgcgaggg gcctgctgct cgcagcctct
       61 cggagtcagc gtccgagcga gcagcgatga tccttgagga gaggccagat ggccagggca
      121 ctggcgagga aagctctcgg ccgcaggacg acggcagcat ccgcaagggc tatggctcct
      181 ttgtgcaaaa ccagcctggc cagctgcaga gtcacagggc tcggctccac cagcagatca
      241 gcaaggagct ccggatgcgg accggtgctg aaaacctcta cagagccacc agcaacacct
      301 gggtccgaga aacagtcgca ctggagctga gctacgtcaa ctccaacctg cagctgctga
      361 aagaggagct ggcagagctg agcaccagcg tagatgtgga ccagccagag ggcgaaggga
      421 ttactattcc catgatcccc ctggggctga aggaaaccaa ggagttggac tgggccacac
      481 cgctgaagga gctgatctct gagcactttg gagaggatgg tacctccttt gagacagaga
      541 tccaggaact agaagaccta cggcaggcca cgaggactcc cagcagggac gaggcgggcc
      601 tggatctgct cgcagcctac tatagccagc tctgtttcct ggacgcacgt ttcttcagtc
      661 cctccaggag ccccgggctg ctcttccact ggtacgactc gctcacgggg gtcccggcac
      721 agcagcgggc tctggccttc gagaagggca gtgtactgtt taacattggg gcactccaca
      781 cgcagattgg agcacggcag gattgctcct gcaccgaggg caccaaccac gctgctgagg
      841 ctttccagag ggctgctggg gccttcagac tcctgaggga gaacttctcc cacgcgccaa
      901 gcccagacat gagtgctgcc tctctctcca tgctggagca gctcatgatt gcccaggcac
      961 aggaatgcat cttcaagggc cttttactgc cagcctctgc cacacctgac atctgtccag
     1021 atcaactgca gctggcccag gaagctgccc aggtggcaac tgaatatggg cttgtgcacc
     1081 gggccatggc ccagccacct gtccgagact acctgcctgc ctcctggact aacctggcgc
     1141 acgtcaaggc tgagcacttc tgcgccctgg cccactatca tgcagccatg gccctctgcg
     1201 aaagccaccc agcagccaag ggtgaactcg caaggcagga gcacgtcttc cagccctcca
     1261 ctccccatga gcccctgggc cccacactgc cgcagcaccc agaagaccgc aggaagcttg
     1321 ccaaggccca cctgaagcgt gccatcctgg gccaggagga ggcgctgcgg cttcacacct
     1381 tgtgcagagt cctgcgcaag gtggacctgc tgcaggttgt ggtgactcag gcactgcggc
     1441 gctccctggc caagtactca cagctcgagc gcgaggatga cttcttcgag gctacggagg
     1501 ccccagacat ccaacccaag actcaccaga ccccagaggt gaggatgcct agcctgtcgc
     1561 aggtgaaggt gactgacttc ttccatcggc tgggaccctt gtctgtgttt tcaaccaaga
     1621 accgatggca gttggtaggg ccggtgcaca tgactcgagg agagggaagc ttcgggttca
     1681 cgctgcgcgg agactctcca gtcctcatcg ctgctgtcgt ccctgggggt caggctgagt
     1741 ccgctggcct gaaggagggc gactacatcg tgtccgtgaa cgggcagccg tgcaagtggt
     1801 ggaagcacct ggaggtggtg acacagctga ggagcatggg cgaggaaggc gtgagcctgc
     1861 aggtcgtgtc attgctgccc agccctgagc cacgtggcac aggaccccga cgggcagcac
     1921 tgctgtggaa ccagagagag tgtggttttg agacaccgat gcccacacgt acccgcccct
     1981 ggcccatcct tggctggagc cgcaagaata aacaaggcaa gactggaagc caccctgacc
     2041 cctgcaccaa caggaactgt gtcacctgcc cctgagctgg acaccccgta tccctgtgtg
     2101 ctcaggatgg ctgtgggggc catactccta tgtacatcac cctggaatca gctccaacta
     2161 gccagatgtg cctagcgggg tctgtcctct actcttgggg actatcaggc tgcccccacc
     2221 ttgcttagag atgtctatta aaaatgctgg tcagatga
//
