LOCUS       NM_133955               3429 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ras homolog family member U (Rhou), mRNA.
ACCESSION   NM_133955 XM_916616
VERSION     NM_133955.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Laurin,M., Gomez,N.C., Levorse,J., Sendoel,A., Sribour,M. and
            Fuchs,E.
  TITLE     An RNAi screen unravels the complexities of Rho GTPase networks in
            skin morphogenesis
  JOURNAL   Elife 8, e50226 (2019)
   PUBMED   31556874
  REMARK    GeneRIF: Coupling our top hit with gain/loss-of-function genetics,
            interactome proteomics and tissue imaging, the authors show that
            RHOU, an atypical Rho, governs the cytoskeletal-junction dynamics
            that establish columnar shape and planar cell polarity in epidermal
            progenitors.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3429)
  AUTHORS   Slaymi,C., Vignal,E., Cres,G., Roux,P., Blangy,A., Raynaud,P. and
            Fort,P.
  TITLE     The atypical RhoU/Wrch1 Rho GTPase controls cell proliferation and
            apoptosis in the gut epithelium
  JOURNAL   Biol Cell 111 (5), 121-141 (2019)
   PUBMED   30834544
  REMARK    GeneRIF: Loss of RhoU function in the mouse gut epithelium
            increases RhoA activity and the level of phosphorylated myosin
            light chain-2. Rhou knockdown in the mouse gut causes cell
            hyperplasia.
REFERENCE   4  (bases 1 to 3429)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3429)
  AUTHORS   Conte,D., Garaffo,G., Lo Iacono,N., Mantero,S., Piccolo,S.,
            Cordenonsi,M., Perez-Morga,D., Orecchia,V., Poli,V. and Merlo,G.R.
  TITLE     The apical ectodermal ridge of the mouse model of ectrodactyly
            Dlx5;Dlx6-/- shows altered stratification and cell polarity, which
            are restored by exogenous Wnt5a ligand
  JOURNAL   Hum Mol Genet 25 (4), 740-754 (2016)
   PUBMED   26685160
REFERENCE   6  (bases 1 to 3429)
  AUTHORS   Ruusala,A. and Aspenstrom,P.
  TITLE     The atypical Rho GTPase Wrch1 collaborates with the nonreceptor
            tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics
  JOURNAL   Mol Cell Biol 28 (5), 1802-1814 (2008)
   PUBMED   18086875
  REMARK    GeneRIF: Wrch1 collaborates with FAK2 and Src in regulating
            cytoskeletal dynamics.
REFERENCE   7  (bases 1 to 3429)
  AUTHORS   Tachikawa,K., Sasaki,S., Maeda,T. and Nakajima,K.
  TITLE     Identification of molecules preferentially expressed beneath the
            marginal zone in the developing cerebral cortex
  JOURNAL   Neurosci Res 60 (2), 135-146 (2008)
   PUBMED   18055048
REFERENCE   8  (bases 1 to 3429)
  AUTHORS   Berzat,A.C., Buss,J.E., Chenette,E.J., Weinbaum,C.A., Shutes,A.,
            Der,C.J., Minden,A. and Cox,A.D.
  TITLE     Transforming activity of the Rho family GTPase, Wrch-1, a
            Wnt-regulated Cdc42 homolog, is dependent on a novel
            carboxyl-terminal palmitoylation motif
  JOURNAL   J Biol Chem 280 (38), 33055-33065 (2005)
   PUBMED   16046391
  REMARK    GeneRIF: Wrch-1 membrane association, subcellular localization, and
            biological activity are mediated by a novel membrane-targeting
            mechanism distinct from that of Cdc42 and other isoprenylated Rho
            family GTPases
REFERENCE   9  (bases 1 to 3429)
  AUTHORS   Daigo,Y., Takayama,I., Ponder,B.A., Caldas,C., Ward,S.M.,
            Sanders,K.M. and Fujino,M.A.
  TITLE     Novel human, mouse and xenopus genes encoding a member of the RAS
            superfamily of low-molecular-weight GTP-binding proteins and its
            downregulation in W/WV mouse jejunum
  JOURNAL   J Gastroenterol Hepatol 19 (2), 211-217 (2004)
   PUBMED   14731133
  REMARK    GeneRIF: G28K was differentially expressed in the small intestines;
            interstitial cells of Cajal within the small intestine expressed
            mRNA for G28K; downregulated in the jejunum of W/W V mice.
REFERENCE   10 (bases 1 to 3429)
  AUTHORS   Tao,W., Pennica,D., Xu,L., Kalejta,R.F. and Levine,A.J.
  TITLE     Wrch-1, a novel member of the Rho gene family that is regulated by
            Wnt-1
  JOURNAL   Genes Dev 15 (14), 1796-1807 (2001)
   PUBMED   11459829
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140552.3.
            
            On Feb 1, 2022 this sequence version replaced NM_133955.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB051827.1, AF378088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               AC140552.3         47214-47560
            348-406             AC140552.3         48836-48894
            407-3429            AC140552.3         54205-57227
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 72.1 cM"
     gene            1..3429
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /note="ras homolog family member U"
                     /db_xref="GeneID:69581"
                     /db_xref="MGI:MGI:1916831"
     exon            1..347
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..862
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /note="Wnt1 responsive Cdc42 homolog; ras homolog gene
                     family, member U; rho GTPase-like protein ARHU; wnt-1
                     responsive Cdc42 homolog 1; Ras-like protein member U"
                     /codon_start=1
                     /product="rho-related GTP-binding protein RhoU"
                     /protein_id="NP_598716.1"
                     /db_xref="CCDS:CCDS22762.1"
                     /db_xref="GeneID:69581"
                     /db_xref="MGI:MGI:1916831"
                     /translation="MAPQQGRPALPARCEPPAAPPVPPRRERGGRGARGPGVSGGRGR
                     AGGAEGRGVKCVLVGDGAVGKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRL
                     QLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPTSFQNVGEKWVPEIRRHCPKAPII
                     LVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEVKAVSYIECSALTQKNLKEVF
                     DAAIVAGIQHSDSQLQPKKSKSRTPDKVRDLSKSWWRKYCCLA"
     exon            348..406
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..3429
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3406..3411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /note="hexamer: AATAAA"
     polyA_site      3429
                     /gene="Rhou"
                     /gene_synonym="2310026M05Rik; Arhu; CDC42L1; G28K; mG28K;
                     WRCH-1; WRCH1"
                     /note="major polyA site"
ORIGIN      
        1 agacgcccag ccgagtgcgg accgtgcgcc gcctttgtcc gccggtggca gctggtctcg
       61 cggccgccgc cccgcgatgg ccccgcagca aggccggccg gcgctgcccg cccgctgcga
      121 gccgccggcg gcgccgccgg taccgcctcg ccgagagcgc ggggggcgcg gggcgcgcgg
      181 gcccggggtg tccgggggtc gggggcgcgc gggcggcgcc gagggacgcg gcgtcaagtg
      241 cgtgctggtc ggcgacggcg cggtgggcaa gaccagcctg gtggtcagct acaccactaa
      301 cggctacccc accgagtaca tccctacggc cttcgacaac ttctcggccg tggtgtctgt
      361 agatgggcgg cctgtgagac tccagctctg tgacactgca ggacaggatg agtttgacaa
      421 gctgaggccc ctctgctaca ccaacacaga catcttcctg ctgtgcttca gcgtggtgag
      481 ccccacatcc ttccagaacg tgggcgagaa gtgggttcca gagattcgac gtcactgccc
      541 aaaggccccc atcatcctgg tcgggacaca gtcggacctc agggaggacg tcaaagtgct
      601 catagaactg gacaagtgca aagagaagcc ggtgcctgaa gaggcggcga agctgtgcgc
      661 ggaggaagtc aaagctgtct cctacatcga gtgctcagcg ttgactcaga aaaacctcaa
      721 agaggttttc gacgccgcca ttgttgctgg tatccagcac tcagactccc agctacagcc
      781 aaagaagtct aaaagcagga ccccggataa ggtgcgggac ctgtccaagt cttggtggag
      841 gaagtattgc tgcctggcct gactctcgca aatagcaggt gtttaagctg caacagctct
      901 ttatggacga ggctgtcata ggatgagccc caaagcaccc tcttctgccc ttaacttcct
      961 gtgtgcggga gcttagggct gagattcata tgcaaaatac gtttttttaa aaattgaaag
     1021 ttacattttt tttctgttaa gtctggaagc tttgagctgt agacctccgg attaatttat
     1081 attccatatg aaaagggctc ttcaaagcgg ggtgtcagca tgaagttctg ctgtgttgta
     1141 caggacaaag gagaatgaat gggaccttct cctgattaag ggctactgag ggctcagtgc
     1201 agggcacgtg tgcaccaggc ttggtgagag tgagcaagcg tgagctttga aaccacacga
     1261 gccacccccg gttttgtaag ggcaaagatc tgaaaccagc aagggccttc tgcttacgaa
     1321 acctcgagcc catcccttct gtttactcag attctcttag gattttaaaa caaccaaaca
     1381 tcccacagcc tactggcata gtgttggcga acagtgcact tgcttgttac ggttttgttt
     1441 tgttttttta aatcacgtga ccagttatat tgctatgaaa atggtggaga tgcctcgtag
     1501 aaggcgagtg ctgggtgcac atgtgacatt ttcttcaggg agcgactcat ggtgagacca
     1561 gagagggctc ttagcttgca ggactggctt ctgcagggca tctgtgtcct gctgttaaaa
     1621 gcaggaggag gtgcttgtct gggagcttta agtgtgctgg gctcatatcg tcccgtttgc
     1681 aaggaattgg gccaccttga gaggccatag ttgatggcta tgggacacac acacactttt
     1741 tccttaagtc caccaaaatg cctgcctgta cacacacaca cacacacaca cacacacaca
     1801 cacacacaca ctggctggtt tgctgatgga acccttagac caccctccca cccccacccc
     1861 tccccaagca tggctgcaag tgtcagggca ccacaccttc ctcttcttga catttctttg
     1921 aacagacatc attttgtagg atcttaattt atacattttt ttcaggtcat aaaatgtggg
     1981 atgaacatac tttgaacccc agtgccttca gggtccattg actagggagg cactgtctta
     2041 ggggacaggt atgtgcaagg ccttacccac cagtggcttc tcgctgcagg tcatgtttgt
     2101 ggcacttgtt ctttaaggtg agggtcttat gaccgactgt tctgagacag ccctgtgtca
     2161 ggcaagctct ttcacagggt tgtaggtatt tccaagacgc cataggaacc agacagtgaa
     2221 tcatagctat cagtttgctg tgggcaagga acctcttttt ggccacctgg taacaaaatt
     2281 ttatgtctgt aaattttttc ttgctattta aaaaaaaaaa tcaatcttac gtttttctgt
     2341 aggaaaaaaa aaaacaagta aaagaacagg ccatatttca ggtcaaaggc ttcttcctgc
     2401 tggtaaatgg gactgaagac tttcttacat cattattaaa aggctaattg ctgaaccact
     2461 agagtatatg aactgtttgt gaatgatatt agccatagtc tcctgaggtg tttccttgtg
     2521 gcctgagtgg taacattgtt ttgcttatgg agatgctgta actgacctag tgactcagct
     2581 tatcctattg tgcatggctg tctggaaagc cagcgtacaa gtggggcttt gcctgccctg
     2641 tgtacagagg gtgggtggga aagagtgaat tatttaattt taaatgttat aataaagcca
     2701 atgtagttga gaccaaggaa atgagcattg agaacacaaa cttgaagtct ggtgccaggg
     2761 ttgttggacc tcacaccctg tctctgagcc acccggaagt gacataaagg acgctgtgtg
     2821 atcaagttct ggacactttt ctgggatgcg taccactgga ctatttatgt cacaaatcta
     2881 gtgggttgac gctgccctgc aagttttcaa tgtccctgca tcctatgaag tcataatgtc
     2941 tgactgtact ggaggttttc ctgcattttt tacttttcga aaatagaggt ttgggctgag
     3001 aattctaaac gcatgtgcct gggtgggacg tcaagtcagg gttctcatca aagctgagaa
     3061 gtggctggaa tgttcagctt ggtgtctggg gaggatcctg tgagctatgt agagaggtgg
     3121 ctcttcagcc tgactcagtg tgggctgaac gaagtacctg cagaacacac ggtagcaggc
     3181 tccaaaatcg tcacctcaag catgcgtgca agcaaacttc cgagaactcc gttttctgct
     3241 cggcagacgt gtgagcagct acccagaagt ctcaagccaa aaggggagcc tcgctcgctg
     3301 gctcctctgc aggtgcctta tcgacctgtg ctcttctctt ttcccgtgtc aaagatgttg
     3361 gacaggatct tgtacttgaa acatactaca aatgagttac tatgaaataa attctgacct
     3421 gtggaccga
//
