LOCUS       NM_145999               2863 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ras homolog family member T2 (Rhot2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145999
VERSION     NM_145999.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Lopez-Domenech,G., Howden,J.H., Covill-Cooke,C., Morfill,C.,
            Patel,J.V., Burli,R., Crowther,D., Birsa,N., Brandon,N.J. and
            Kittler,J.T.
  TITLE     Loss of neuronal Miro1 disrupts mitophagy and induces
            hyperactivation of the integrated stress response
  JOURNAL   EMBO J 40 (14), e100715 (2021)
   PUBMED   34152608
REFERENCE   3  (bases 1 to 2863)
  AUTHORS   Modi,S., Lopez-Domenech,G., Halff,E.F., Covill-Cooke,C.,
            Ivankovic,D., Melandri,D., Arancibia-Carcamo,I.L., Burden,J.J.,
            Lowe,A.R. and Kittler,J.T.
  TITLE     Miro clusters regulate ER-mitochondria contact sites and link
            cristae organization to the mitochondrial transport machinery
  JOURNAL   Nat Commun 10 (1), 4399 (2019)
   PUBMED   31562315
  REMARK    GeneRIF: Using knockout mouse embryonic fibroblasts, data show that
            mitochondrial Rho (Miro) GTPases Miro1 and Miro2 are required for
            normal mitochondrial cristae architecture and endoplasmic
            reticulum-mitochondria contacts Sites (ERMCS).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2863)
  AUTHORS   Cao,Y., Xu,C., Ye,J., He,Q., Zhang,X., Jia,S., Qiao,X., Zhang,C.,
            Liu,R., Weng,L., Liu,Y., Liu,L. and Zheng,M.
  TITLE     Miro2 Regulates Inter-Mitochondrial Communication in the Heart and
            Protects Against TAC-Induced Cardiac Dysfunction
  JOURNAL   Circ Res 125 (8), 728-743 (2019)
   PUBMED   31455181
  REMARK    GeneRIF: Mitochondrial Miro2 expression levels regulate
            inter-mitochondrial communication along microtubules in adult
            cardiomyocytes.  Degradation of Miro2 through Parkin-mediated
            ubiquitination contributes to impaired inter-mitochondrial
            communication and cardiac dysfunction during hypertrophic heart
            diseases.
REFERENCE   5  (bases 1 to 2863)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2863)
  AUTHORS   Lopez-Domenech,G., Covill-Cooke,C., Ivankovic,D., Halff,E.F.,
            Sheehan,D.F., Norkett,R., Birsa,N. and Kittler,J.T.
  TITLE     Miro proteins coordinate microtubule- and actin-dependent
            mitochondrial transport and distribution
  JOURNAL   EMBO J 37 (3), 321-336 (2018)
   PUBMED   29311115
  REMARK    GeneRIF: both Miro proteins can act to coordinate
            microtubule-dependent and actin-dependent mitochondrial
            trafficking, Miro1 preferentially acts to control
            microtubule-dependent trafficking through kinesin and dynein, while
            Miro2 plays a more prominent role in coordinating mitochondrial
            interactions with the actin cytoskeleton through a more efficient
            recruitment and stability of endogenous Myo19 in the mitochondrial
            membrane.
REFERENCE   7  (bases 1 to 2863)
  AUTHORS   Lopez-Domenech,G., Higgs,N.F., Vaccaro,V., Ros,H.,
            Arancibia-Carcamo,I.L., MacAskill,A.F. and Kittler,J.T.
  TITLE     Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse
            Model with Disrupted Mitochondrial Distribution in Mature Dendrites
  JOURNAL   Cell Rep 17 (2), 317-327 (2016)
   PUBMED   27705781
  REMARK    GeneRIF: Using mouse knockout strategies, we demonstrate that
            Miro1, as opposed to Miro2, is the primary regulator of
            mitochondrial transport in both axons and dendrites. Miro1 deletion
            leads to depletion of mitochondria from distal dendrites but not
            axons, accompanied by a marked reduction in dendritic complexity.
            Disrupting postnatal mitochondrial distribution in vivo by deleting
            Miro1 in mature neurons causes a progressive loss
REFERENCE   8  (bases 1 to 2863)
  AUTHORS   Liakath-Ali,K., Vancollie,V.E., Heath,E., Smedley,D.P., Estabel,J.,
            Sunter,D., Ditommaso,T., White,J.K., Ramirez-Solis,R., Smyth,I.,
            Steel,K.P. and Watt,F.M.
  TITLE     Novel skin phenotypes revealed by a genome-wide mouse reverse
            genetic screen
  JOURNAL   Nat Commun 5, 3540 (2014)
   PUBMED   24721909
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2863)
  AUTHORS   Lopez-Domenech,G., Serrat,R., Mirra,S., D'Aniello,S., Somorjai,I.,
            Abad,A., Vitureira,N., Garcia-Arumi,E., Alonso,M.T.,
            Rodriguez-Prados,M., Burgaya,F., Andreu,A.L., Garcia-Sancho,J.,
            Trullas,R., Garcia-Fernandez,J. and Soriano,E.
  TITLE     The Eutherian Armcx genes regulate mitochondrial trafficking in
            neurons and interact with Miro and Trak2
  JOURNAL   Nat Commun 3, 814 (2012)
   PUBMED   22569362
  REMARK    GeneRIF: Armcx genes regulate mitochondrial trafficking in neurons
            and interact with Miro 1/2 and Trak2.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2863)
  AUTHORS   Shan,Y., Hexige,S., Guo,Z., Wan,B., Chen,K., Chen,X., Ma,L.,
            Huang,C., Zhao,S. and Yu,L.
  TITLE     Cloning and characterization of the mouse Arht2 gene which encodes
            a putative atypical GTPase
  JOURNAL   Cytogenet Genome Res 106 (1), 91-97 (2004)
   PUBMED   15218247
  REMARK    GeneRIF: molecular cloning and characterization of a novel mouse
            cDNA encoding a putative atypical GTPase protein, Arht2
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY311390.1 and AC159277.3.
            
            On Jul 17, 2018 this sequence version replaced NM_145999.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY311390.1, BC029777.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2482              AY311390.1         327-2808
            2483-2863           AC159277.3         194531-194911
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.93 cM"
     gene            1..2863
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /note="ras homolog family member T2"
                     /db_xref="GeneID:214952"
                     /db_xref="MGI:MGI:2384892"
     exon            1..126
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..1952
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /EC_number="3.6.5.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial Rho 2; mitochondrial Rho GTPase 2; ras
                     homolog gene family, member T2"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 2 isoform 1"
                     /protein_id="NP_666111.1"
                     /db_xref="CCDS:CCDS37505.1"
                     /db_xref="GeneID:214952"
                     /db_xref="MGI:MGI:2384892"
                     /translation="MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPARAEEITIPA
                     DVTPEKVPTHIVDYSEAEQTEEELQEEIHKANVVCVVYDVSEETTIEKIRTKWIPLVN
                     GRTATGPRLPIILVGNKSDLRPGSTMEAVLPIMSQFPEIETCVECSAKHLRNISELFY
                     YAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDRDHGLSDEELNAFQKSCFG
                     HPLAPQALEDVKRVVCKNVSGGVQNDRLTLEGFLFLNTLFIQRGRHETTWTILRRFGY
                     SDSLELTPDYLYPALHVPPGCSTELNHRGYQFVQRMFEKHDQDHDGVLSPTELQNLFS
                     VFSGAPWGPELLHTVPTQAGCLPLHGYLCQWTLMTYLDVQQCLAHLGYLGYPTLCEQD
                     SQAQAITVTREKKLDQEKGQTQRSVLMCKVLGARGVGKSAFLQAFLGNSLGEARDPPE
                     KFPLHTINTVRVNGQEKYLILCEVNADSLLDTSLDTTCDVACLMFDSSDPKTFVHCAT
                     IYKRYYMDGQTPCLFIASKADLPEGVAPPGLSPAEFCRRHRLPAPASFSCLGPAMPST
                     DVFTQLATMATFPHLVHTELHPTSFWLRGVLVAVGTAVAAVLSFSLYRVLVKSR"
     exon            127..185
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            186..267
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            268..311
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            312..365
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            366..418
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            419..527
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            528..629
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            630..728
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            729..837
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            838..958
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1043
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1186
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1287
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1418
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1499
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1622
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1825
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..2863
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2459..2464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /note="hexamer: ATTAAA"
     polyA_site      2482
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /note="major polyA site"
     regulatory      2838..2843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /note="hexamer: CATAAA"
     polyA_site      2863
                     /gene="Rhot2"
                     /gene_synonym="Arht2; Miro2"
                     /note="major polyA site"
ORIGIN      
        1 gaaggagagg gttggggttg ggattgggtc tacttgggcc gggccgggcc gggaggggtg
       61 ggtttggggt gctgctgcgg gcagcagcca tgcggcgcga tgttcgcatc ctgctgctgg
      121 gcgaggccca ggtggggaag acgtctctga tcctgtcgct ggtcggcgag gagtttcccg
      181 aggaggtccc tgcccgcgcg gaggagataa ccatccccgc cgacgtcacc ccggagaagg
      241 tgcccactca tatcgtggat tactcagaag cggagcagac agaggaagaa cttcaggagg
      301 agatccacaa ggcaaatgta gtgtgtgtgg tgtacgatgt gtctgaggag accaccattg
      361 agaagatccg aaccaagtgg atcccgcttg tgaatggcag gacggcgaca gggcccaggt
      421 tgcccatcat cctggtgggc aataagtctg acctgcggcc ggggagtacc atggaggctg
      481 tgctgcccat catgagccag tttcctgaga tagagacctg cgtggagtgt tcagccaagc
      541 acttgaggaa tatctcagag ctgttctact atgcccagaa ggctgttctg catcccacag
      601 ccccccttta tgaccctgag gccaaacagc tcaggcctgc atgcgcccag gcgctcacac
      661 gcatcttcag gctgtcggac caggaccgag accacgggct gagcgacgag gaactcaatg
      721 ccttccagaa gtcctgcttt ggccatcccc tggccccaca ggccctggag gatgtgaaga
      781 gggtggtgtg caagaacgtg tcaggtggtg tgcagaatga ccggctgacc ctggaaggct
      841 tcctcttcct gaacacactc ttcatccagc gtggccggca cgagaccacg tggaccatcc
      901 tgcggcgctt tggttacagt gactcactgg agctaacgcc cgactacctc tatccagcgc
      961 tccatgtccc ccctggctgc agcacagagc tcaaccatcg tggctaccag tttgtgcagc
     1021 ggatgttcga gaagcatgac caggaccatg atggtgtcct ctcgcccacg gaactacaga
     1081 atctcttcag tgtgttctca ggtgctccat ggggccctga actcttacac acagtcccca
     1141 ctcaggctgg ctgtctgcct ttgcatggct atctctgcca gtggaccctc atgacctact
     1201 tggatgtcca gcaatgcctt gcacaccttg gctacctggg ttaccctacc ctctgtgaac
     1261 aggactctca ggcacaggct atcacagtta cccgcgagaa gaagctggac caggagaaag
     1321 ggcagacaca gcgcagcgtt ctcatgtgta aggtgctggg agcccgagga gtgggcaagt
     1381 cagccttctt gcaagccttc cttggcaaca gccttgggga agccagggac ccccctgaga
     1441 aattccccct gcataccatc aatacagtgc gggtcaacgg gcaggagaag tacctgattc
     1501 tgtgtgaagt gaatgctgac agcctgctgg acacctctct agacactacc tgtgatgtcg
     1561 cctgcttaat gtttgacagc agtgatccca agacctttgt acactgtgct accatataca
     1621 agcgttatta catggatggg cagaccccct gcctctttat tgcctccaaa gctgatctcc
     1681 ctgaaggtgt tgccccccca gggctgtcac ccgctgagtt ctgccggagg caccggctgc
     1741 ctgccccagc ttcattctcc tgcttgggac ccgcaatgcc cagcacagat gtctttaccc
     1801 aacttgctac catggccact ttcccacacc tggtgcacac agagctgcac cccacctcct
     1861 tctggctccg aggagtgctg gtggctgtcg gcacggctgt ggctgctgtc cttagcttct
     1921 cactatacag ggtcctagtg aagagccgat gacaaaggac ccatgagcct ctcctgcccc
     1981 caggcacagc acactgtggt gccttatgtg ggcccacacg gcaaagaagt atgggctttc
     2041 ggcctgttgg agtaatatct gtgtggctgg ctacagggtc cctgggactg atgtgggttc
     2101 ctgctcagga acttggtatt tagatcttgg cttccaaaag tctgttgccc tattccaggc
     2161 ctgtcctgct gagcctgaga gtactggtgc caggtgtgag gccttagcct tggagggagg
     2221 gaggattgtg tgtaaggggc tgggctgtgg gtaaggaatc cagtgaccag gacccgaagt
     2281 tctcagggtg tgttcccccc ccttcctggt tgtgcatggc cagtggacat gtagagggca
     2341 agaggtagtg gtcaaatcag ggagggaccc gatagtcaca gaggtcccac ctttttgtca
     2401 ggtgtggagc cttatccaca ctgcgccagc tgcagagcaa gcggaactga ctttcctgat
     2461 taaatttcac ttgtcttttc catctcttgg attttgatct ctcaatgcaa cttactttct
     2521 ctctttctct ccctcccttc cttccttcct ttcttccttc ttcctttctt ctttctttct
     2581 ttctttcttt ctttctttct ttctttcttt ctttcaaaaa tatgcttatt tttatgagtt
     2641 tgagtgtttt gcctgcccat atatgtaagt gccccgtgtg tgtgcctggt accgacagaa
     2701 gccaaatgag tatgtcagag ttccctggaa ctggagttaa ctaatgattg tgagccatcc
     2761 cgtgggtgtt ggaaacaaac ccaggtcctc tgcagaggca gcaagtgctc tgaaccacca
     2821 agtcctctct ccagccccat aaaaaatggc ttttcaatcg caa
//
