LOCUS       NM_001404128            4474 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus ras homolog family member T1 (Rhot1), transcript
            variant 7, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001404128 XM_006533886
VERSION     NM_001404128.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ding,P., Gao,C., Zhou,J., Mei,J., Li,G., Liu,D., Li,H., Liao,P.,
            Yao,M., Wang,B., Lu,Y., Peng,X., Jiang,C., Yin,J., Huang,Y.,
            Zheng,M., Gao,Y., Zhang,C. and Gao,J.
  TITLE     Mitochondria from osteolineage cells regulate myeloid cell-mediated
            bone resorption
  JOURNAL   Nat Commun 15 (1), 5094 (2024)
   PUBMED   38877020
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4474)
  AUTHORS   Zhou,H., Zhang,W., Li,H., Xu,F., Yinwang,E., Xue,Y., Chen,T.,
            Wang,S., Wang,Z., Sun,H., Wang,F., Mou,H., Yao,M., Chai,X.,
            Zhang,J., Diarra,M.D., Li,B., Zhang,C., Gao,J. and Ye,Z.
  TITLE     Osteocyte mitochondria inhibit tumor development via
            STING-dependent antitumor immunity
  JOURNAL   Sci Adv 10 (3), eadi4298 (2024)
   PUBMED   38232158
REFERENCE   4  (bases 1 to 4474)
  AUTHORS   Li,J.M., Li,X., Chan,L.W.C., Hu,R., Zheng,T., Li,H. and Yang,S.
  TITLE     Lipotoxicity-polarised macrophage-derived exosomes regulate
            mitochondrial fitness through Miro1-mediated mitophagy inhibition
            and contribute to type 2 diabetes development in mice
  JOURNAL   Diabetologia 66 (12), 2368-2386 (2023)
   PUBMED   37615690
  REMARK    GeneRIF: Lipotoxicity-polarised macrophage-derived exosomes
            regulate mitochondrial fitness through Miro1-mediated mitophagy
            inhibition and contribute to type 2 diabetes development in mice.
REFERENCE   5  (bases 1 to 4474)
  AUTHORS   Xue,Y., Meng,T.G., Ouyang,Y.C., Liu,S.L., Guo,J.N., Wang,Z.B.,
            Schatten,H., Song,C.Y., Guo,X.P. and Sun,Q.Y.
  TITLE     Miro1 regulates mitochondrial homeostasis and meiotic resumption of
            mouse oocyte
  JOURNAL   J Cell Physiol 237 (12), 4477-4486 (2022)
   PUBMED   36183380
  REMARK    GeneRIF: Miro1 regulates mitochondrial homeostasis and meiotic
            resumption of mouse oocyte.
REFERENCE   6  (bases 1 to 4474)
  AUTHORS   Ahmad,T., Mukherjee,S., Pattnaik,B., Kumar,M., Singh,S., Kumar,M.,
            Rehman,R., Tiwari,B.K., Jha,K.A., Barhanpurkar,A.P., Wani,M.R.,
            Roy,S.S., Mabalirajan,U., Ghosh,B. and Agrawal,A.
  TITLE     Miro1 regulates intercellular mitochondrial transport & enhances
            mesenchymal stem cell rescue efficacy
  JOURNAL   EMBO J 33 (9), 994-1010 (2014)
   PUBMED   24431222
  REMARK    GeneRIF: Miro1 overexpression leads to increased stem cell repair.
REFERENCE   7  (bases 1 to 4474)
  AUTHORS   Lopez-Domenech,G., Serrat,R., Mirra,S., D'Aniello,S., Somorjai,I.,
            Abad,A., Vitureira,N., Garcia-Arumi,E., Alonso,M.T.,
            Rodriguez-Prados,M., Burgaya,F., Andreu,A.L., Garcia-Sancho,J.,
            Trullas,R., Garcia-Fernandez,J. and Soriano,E.
  TITLE     The Eutherian Armcx genes regulate mitochondrial trafficking in
            neurons and interact with Miro and Trak2
  JOURNAL   Nat Commun 3, 814 (2012)
   PUBMED   22569362
  REMARK    GeneRIF: Armcx genes regulate mitochondrial trafficking in neurons
            and interact with Miro 1/2 and Trak2.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4474)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 4474)
  AUTHORS   Jenne,D.E., Tinschert,S., Dorschner,M.O., Hameister,H., Stephens,K.
            and Kehrer-Sawatzki,H.
  TITLE     Complete physical map and gene content of the human NF1 tumor
            suppressor region in human and mouse
  JOURNAL   Genes Chromosomes Cancer 37 (2), 111-120 (2003)
   PUBMED   12696059
REFERENCE   10 (bases 1 to 4474)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591426.17.
            
            On Apr 8, 2022 this sequence version replaced XM_006533886.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784647.1364895.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-240               AL591426.17        28011-28250
            241-299             AL591426.17        39185-39243
            300-381             AL591426.17        42830-42911
            382-425             AL591426.17        44513-44556
            426-479             AL591426.17        44990-45043
            480-532             AL591426.17        46207-46259
            533-641             AL591426.17        52309-52417
            642-743             AL591426.17        52856-52957
            744-842             AL591426.17        60948-61046
            843-951             AL591426.17        61500-61608
            952-1072            AL591426.17        62297-62417
            1073-1157           AL591426.17        64929-65013
            1158-1303           AL591426.17        65594-65739
            1304-1404           AL591426.17        67365-67465
            1405-1535           AL591426.17        69135-69265
            1536-1619           AL591426.17        69970-70053
            1620-1739           AL591426.17        71894-72013
            1740-1942           AL591426.17        73587-73789
            1943-2038           AL591426.17        74789-74884
            2039-2161           AL591426.17        76441-76563
            2162-2305           AL591426.17        83171-83314
            2306-4474           AL591426.17        84669-86837
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 47.62 cM"
     gene            1..4474
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="ras homolog family member T1"
                     /db_xref="GeneID:59040"
                     /db_xref="MGI:MGI:1926078"
     exon            1..240
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..2423
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="isoform 7 is encoded by transcript variant 7;
                     mitochondrial Rho GTPase 1; ras homolog gene family,
                     member T1"
                     /codon_start=1
                     /product="mitochondrial Rho GTPase 1 isoform 7"
                     /protein_id="NP_001391057.1"
                     /db_xref="GeneID:59040"
                     /db_xref="MGI:MGI:1926078"
                     /translation="MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPE
                     EVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHS
                     IDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVEC
                     SAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLN
                     DAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGR
                     HETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRD
                     CALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLE
                     YLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQS
                     LLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLV
                     YDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPP
                     PQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTA
                     FHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRLKARVAEWGAMLLADD
                     EGSITHQVHAVSSVEDSIFMESSHGQLFLDDRHVTQADLKSSTFWLRASFGATVFAVV
                     GFAMYRALLKQR"
     exon            241..299
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            300..381
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            382..425
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            426..479
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            480..532
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            533..641
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            642..743
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            744..842
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            843..951
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1072
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1157
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1303
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1404
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1535
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1619
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1739
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1942
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2038
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2161
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2305
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..4474
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3310..3315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="hexamer: AATAAA"
     polyA_site      3331
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
     regulatory      3352..3357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="hexamer: AATAAA"
     polyA_site      3376
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="major polyA site"
     regulatory      3764..3769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="hexamer: ATTAAA"
     polyA_site      3783
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
     regulatory      4033..4038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="hexamer: AATAAA"
     polyA_site      4066
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
     regulatory      4455..4460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
                     /note="hexamer: ATTAAA"
     polyA_site      4474
                     /gene="Rhot1"
                     /gene_synonym="2210403N23Rik; Arht1; C430039G08Rik; Miro1"
ORIGIN      
        1 ggcttgcccc ggctcccaag cggagcggta gaggaggagt cggtgcggca ggtcctggcg
       61 gccgagattc gggcgcagag gccgctaagg ctggcaggga gcggcggtat cgcggggaga
      121 gcggcgccac gggctggacg ctcccggcgt gaggcgtgcg ctccatgcgt gcgggccgag
      181 tgcggcccct gcgagcctcc gacatgaaga aagacgtgag gatcctgctg gtgggagaac
      241 ctcgagttgg gaagacatca ctgattatgt cgctggtcag tgaagaattt ccggaagagg
      301 ttcctccccg agcagaagaa atcaccattc cagctgacgt cacccctgaa agagttccaa
      361 cacacattgt cgattattca gaagcagaac agagtgatga acaacttcat caagaaatat
      421 cacaggctaa tgttatctgc atagtttatg ctgttaacaa caaacattct attgataagg
      481 taacaagccg atggattcct ctcataaatg aaagaacaga caaagacagc aggctgcctt
      541 tgatattggt tgggaacaaa tctgatctgg tggaatatag tagtatggag accatccttc
      601 caattatgaa ccagtataca gagatagaga cctgtgtgga gtgttcagca aaaaacctaa
      661 agaatatctc agagctgttt tattatgcac agaaagctgt tcttcatcct acagggcctc
      721 tatactgccc agaggagaaa gagatgaaac cggcttgtat aaaggccctc actcgtatat
      781 ttaaaatatc tgatcaggat aacgacggca ctctgaatga tgctgagctc aacttcttcc
      841 agagaatttg tttcaacact cctttagctc cgcaagcgct ggaagatgtc aagaatgtgg
      901 ttagaaagca cttaagtgac ggtgtggctg acagtgggct gacgctcaga ggttttctct
      961 ttttacatac actttttatc cagaggggga ggcatgagac tacttggact gtgcttcgac
     1021 gatttggtta tgacgatgac ctggacctga cgcctgagta tttattcccc ctgctaaaaa
     1081 tacctcctga ttgcactact gaattaaatc atcatgcata tttgtttctc cagagcacct
     1141 ttgacaaaca tgatttggac agagactgtg ctttgtcgcc tgatgaactt aaagatttat
     1201 ttcaagtttt tccttacata ccttggggac cagatgtaaa taacacagtt tgcacaaatg
     1261 aaagaggctg gataacctac caggggttcc tctcccagtg gacgctcacc acctacttag
     1321 atgtgcagcg ttgcctggag tatttgggat atctaggcta ttccatactg acggaacaag
     1381 agtctcaagc ttcagccatt acagtaacaa gggataaaaa gattgacctg cagaaaaaac
     1441 agactcagag aaatgtgttc aggtgtaatg taattggagt gaaaggctgt gggaaaactg
     1501 gagttcttca gtctctcctt ggaagaaacc taatgaggca gaagaaaatt cgtgatgatc
     1561 ataagtccta ttatgcaatt aacactgtct atgtatatgg acaagagaaa tacttgttgc
     1621 tgcacgatat ctcagagtcg gaatttctaa ctgaggctga gaccatttgt gatgttgtat
     1681 gcctggtata tgatgtcacc aatcccaaat cctttgaata ctgtgccagg atttttaagc
     1741 aacattttat ggacagcaga attccctgct tgattgtagc tgcaaagtca gacctgcatg
     1801 aagtcaaaca ggaacatagc atctcaccta ctgatttctg caggaaacac aaaatgcctc
     1861 cacctcaagc cttcacttgc aatactgctg atgcccccag taaggatatc tttgttaagt
     1921 tgacaacaat ggccatgtac ccagaggatc attacagagg cagcctctcc cgagacatgg
     1981 gcagcactga tagaatagag aatttgagaa aaatctgggt ctttctaaaa actgctttcc
     2041 atgcccggtt acgctgcatg tgcacctgca acaggtgtac attttgcatc tgtcagaact
     2101 tcctcaactc agacttgctg caatctgtaa agaacaaaat cttcactgca gttcttaaca
     2161 ggttaaaagc ccgggtagct gagtggggtg ccatgctgtt agcagacgac gaaggcagta
     2221 ttactcacca ggtgcacgcc gtcagctccg tagaagattc catcttcatg gagtcatctc
     2281 acggtcagct ttttcttgat gacaggcacg tgacacaagc tgacctcaag agctcaacgt
     2341 tctggcttcg cgcaagcttt ggtgctacgg tctttgcagt cgtgggcttt gctatgtaca
     2401 gagcactact gaaacagcga tgattaaggg aggcaccggc ctaccaaaac acatacttct
     2461 atgtacattc tgaatgcttt aaattctgct agaatattga gatatttata catgcagagt
     2521 tactttatta atatttgtaa ttcatgcata agagtatttt actcctagta atgactgcag
     2581 tattgactgg catgtggaaa gaaaacatct taggagccaa atataagtgg ctaaatttca
     2641 gaggccaaaa gggaatattt tgtaaataat gtacatattc aagcaagata cggtctcctg
     2701 aactgacttc agaaatgtgt ttccaggcat ttctacagta gtgttctagt gttcacttgg
     2761 ctcaccctgc taggtttcag tcacccagag attgtgttta ctcaggcatc acgtatttat
     2821 ttcaaggtga ctcagaacaa tccttcggct tctgtagccg tttctctcca tttttaagat
     2881 cacacaggag acgaggtcag ctttccaaaa ccactctgtt tccgtcactt gcggtaggcc
     2941 ggcttcaaag ccatcatgaa acctagaaag ttgtggagct gagtatgtgg ctgagggcct
     3001 gaggcaggag caggtggcgg aggttacctg tgtcaccaca ttgcttcttt gtcagagtgt
     3061 ggattttata gccctttcaa tcaaatgggg aaaacagttt gtatattatc aatgttttta
     3121 gttacaataa agaagccgcc ctgaccactg ctgaattccc acctgaagtg aaaccgtcct
     3181 cgaatggccg tgtctgtgta gttattgccg tagctgcatg tgtcatgatg tgttctgtat
     3241 ctggccagga taacctagag gcaacacttt tttaatggta aaataaatct aatgaatata
     3301 aactctcaca ataaacctat tttttccatc attgaccttt tcaagtattt aaataaataa
     3361 ctgctgtgta ctgtgatctt gaggtttttt gtcttctgag gtaaatatct ctgtccagat
     3421 gtgagtgtgc tcctttggtt tctacctttc ttagtttaga cattattttt atacaaaaaa
     3481 gtactactcg gtaggaagga agttgtagga tgtgtgtagg agatctactt agagggctgt
     3541 gacggcgatc tctgaggtct gcctgccctg cctgcccttc tgactctgtc atggtcgtct
     3601 actccttgtc atgatggcgc ttccctctgt tgttggaagg atattttaca caagatcacc
     3661 cttccttagc aacagtaacg acttcgctgg ttacaaacct gttcacctgc ttgttcccct
     3721 ctcctccttc ttcccatttt cattctaaat ttgaagaaaa aaaattaaac gtggagaacc
     3781 tcatttttat tttccttatt attattttta ttacatttgt ctttattttc tgtatctgtg
     3841 tgtacgtgga ccagtgtgtg ccatggcctg tgtgtggcgc tcagagtcga gttagaggag
     3901 tcagttctct ggtacagtgt gggtcttggt gatagaactt agaacatcag gcttggtaaa
     3961 cagatgcctt tatctgctga gccatctttg gcccttaatt ttgattgttt tctgagaatt
     4021 ttccatgtat caaataaatt gatctcagtt atacactttt caaagacaac tgtttctttc
     4081 cttctgcatt tctctttgct ccaactaaaa tattgtttca aataaatagg cctaatagat
     4141 ggctgtgttt gtgagaagca ggaaagaaag taatttctgg agaggtggaa agtggccggg
     4201 cacacacctt taatcccagc actcaggagg cagagggaga ctaatggctg tgttctagtt
     4261 cagagaatcc gttcttaaag gtaggtgggg tgagtgtgtg tggaaagtgc tgggtttaat
     4321 attcaaactt ttttttttaa tcttgttttt cgttcgtttt ggggtttttg agacagggtc
     4381 ttacttatgt actttgactg gccttgaact ccctttgttg accagaaggc ttcggattta
     4441 tagatgtacc tctgattaaa gtcttaaagg caaa
//
