LOCUS       NM_001252637            4433 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rho-related BTB domain containing 1 (Rhobtb1),
            transcript variant 3, mRNA.
ACCESSION   NM_001252637
VERSION     NM_001252637.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Mukohda,M., Fang,S., Wu,J., Agbor,L.N., Nair,A.R., Ibeawuchi,S.C.,
            Hu,C., Liu,X., Lu,K.T., Guo,D.F., Davis,D.R., Keen,H.L.,
            Quelle,F.W. and Sigmund,C.D.
  TITLE     RhoBTB1 protects against hypertension and arterial stiffness by
            restraining phosphodiesterase 5 activity
  JOURNAL   J Clin Invest 129 (6), 2318-2332 (2019)
   PUBMED   30896450
  REMARK    GeneRIF: RhoBTB1 protected from hypertension, vascular smooth
            muscle dysfunction, and arterial stiffness in at least two models
            of hypertension.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4433)
  AUTHORS   Lutz,J., Grimm-Gunter,E.M., Joshi,P. and Rivero,F.
  TITLE     Expression analysis of mouse Rhobtb3 using a LacZ reporter and
            preliminary characterization of a knockout strain
  JOURNAL   Histochem Cell Biol 142 (5), 511-528 (2014)
   PUBMED   24923387
REFERENCE   4  (bases 1 to 4433)
  AUTHORS   Schenkova,K., Lutz,J., Kopp,M., Ramos,S. and Rivero,F.
  TITLE     MUF1/leucine-rich repeat containing 41 (LRRC41), a substrate of
            RhoBTB-dependent cullin 3 ubiquitin ligase complexes, is a
            predominantly nuclear dimeric protein
  JOURNAL   J Mol Biol 422 (5), 659-673 (2012)
   PUBMED   22709582
REFERENCE   5  (bases 1 to 4433)
  AUTHORS   Ramos,S., Khademi,F., Somesh,B.P. and Rivero,F.
  TITLE     Genomic organization and expression profile of the small GTPases of
            the RhoBTB family in human and mouse
  JOURNAL   Gene 298 (2), 147-157 (2002)
   PUBMED   12426103
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB611014.1, BC137646.1 and AC117209.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC137647.1, SRR7345562.2389833.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               BB611014.1         3-172
            171-2352            BC137646.1         1-2182
            2353-4433           AC117209.3         163936-166016
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 36.04 cM"
     gene            1..4433
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="Rho-related BTB domain containing 1"
                     /db_xref="GeneID:69288"
                     /db_xref="MGI:MGI:1916538"
     exon            1..182
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            183..233
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            234..435
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..2331
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="isoform 1 is encoded by transcript variant 3;
                     rho-related BTB domain-containing protein 1"
                     /codon_start=1
                     /product="rho-related BTB domain-containing protein 1
                     isoform 1"
                     /protein_id="NP_001239566.1"
                     /db_xref="CCDS:CCDS35930.1"
                     /db_xref="GeneID:69288"
                     /db_xref="MGI:MGI:1916538"
                     /translation="MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQ
                     LLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSISLRLWDTFGDHHKDRRFAYGRSDV
                     VVLCFSIANPNSLNHVKTMWYQEIKHFCPRTPVVLVGCQLDLRYADLEAVNRARRPLA
                     RPIKRGDILPPEKGREVAKELGIPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWK
                     SHLKKVQKPLLQAPFLPPKAPPPVIKVPECPSAGTSDAACLLDNPLCADVLFVLHDEE
                     HIFAHRIYLATSSSKFYDLFLMECEESPCWGGGAGEEVPCRDFQGRTQSLGSAEEGKE
                     GPQRTPQADPGASSGQDLPESLALQMEASGSEGHALSGWSKGFVSMHREVRVNPISKR
                     VGPVTVVRLDPSMQSGPFRTLLRFLYSGQLDEKEKDLLGLAQMAEVLEMFDLRMMVEN
                     IMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFTLDDGAISAHKPLLICSCEW
                     MAAMFGGSFVESANREVHLPNINKMSMQAVLEYLYTKQLSPNLDLDPLELIALANRFC
                     LTHLVALVEQHAVQELTKAAVSGVSIDGEVLSYLELAQFHNANQLAAWCLHHICTNYN
                     SVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDLALNKHHSR
                     RKWCFWHSSPAVA"
     exon            436..539
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            540..725
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            726..1696
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1815
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1966
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2055
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2161
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..4433
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2508..2513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="hexamer: CATAAA"
     polyA_site      2544
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="major polyA site"
     regulatory      2626..2631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="hexamer: AAGAAA"
     regulatory      2636..2641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="hexamer: AACAAA"
     polyA_site      2646
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="major polyA site"
     polyA_site      2667
                     /gene="Rhobtb1"
                     /gene_synonym="1700008H16Rik; 3110048G13Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggggacgagt cccgtggcct gcgccgggac gcggacgcgg ccgggtgcgg ctgggccatg
       61 ctgcctttgt gccacgccgg gcgggtggcg cgcggtgcgc agggtcccgg cgtccccgcg
      121 agcgcgcgcg gcgggggccg ctgctgagcg gcggagggag ccaggtctcg ctcggctgca
      181 gggtaagacc agctgtgcgc aggaactcct gttcttcact tcccgggagc gagagctttg
      241 taaatggact ctgacatgga ctacgaacga cccaacgttg agaccatcaa gtgcgtggtc
      301 gtgggcgaca acgccgtagg gaagacgcgc ctcatctgtg cccgggcctg caacaccacc
      361 ctgacacagt accagctgct ggccacccat gtgcccaccg tgtgggccat tgatcagtac
      421 cgtgtctgcc aggaggtcct ggagcggtcc agggatgtcg tcgatgaagt gagcatctcc
      481 ctcaggcttt gggacacgtt tggagatcac cacaaagaca ggcggttcgc atatggcagg
      541 tccgatgttg tggtcctctg ctttagcatc gcgaacccca actccctaaa tcatgtgaaa
      601 accatgtggt atcaagaaat caagcacttt tgccctcgaa cgcccgtcgt cctggttggc
      661 tgccagctag acctccgcta cgccgacctg gaggctgtta accgggccag acgcccctta
      721 gcaaggccca taaagagagg tgacattttg cccccagaaa aaggccgcga ggtggcgaag
      781 gaacttggca tcccgtacta cgagaccagc gtgtttgacc agtttggcat caaggacgtg
      841 ttcgacaatg ccatccgagc agccctcatc tcccgccggc acctgcagtt ctggaaatcc
      901 cacttgaaaa aagtccagaa gcctctgctt caggcgccct tcctacctcc gaaagctcct
      961 cccccggtca tcaaggtccc cgagtgtccg tctgcgggga ccagcgatgc tgcctgttta
     1021 ctggacaacc ccctgtgcgc cgacgtcctc ttcgttctcc atgacgagga gcacatcttt
     1081 gcgcaccgca tttacctcgc tacctcttct tccaaattct acgacctttt tttaatggag
     1141 tgtgaagaat ccccttgttg gggtggagga gctggtgagg aggtgccgtg cagggatttt
     1201 caggggcgga cgcagagtct tggatcagcc gaggaaggga aggagggccc ccagaggacc
     1261 cctcaggctg acccaggagc gtcttcaggc caggacctgc ctgagagttt ggctttgcag
     1321 atggaagcct cgggttccga gggacacgcc ctgtccggct ggagcaaggg gtttgtcagc
     1381 atgcacaggg aggtgcgggt caaccccatt tccaagcggg tgggcccagt gaccgtggtc
     1441 aggctggacc catcgatgca gtcaggccct ttccggaccc tgctgagatt cctctactct
     1501 gggcagctgg acgaaaagga aaaagatctg ctggggctgg ctcagatggc cgaggtcctg
     1561 gagatgtttg atctgaggat gatggtagag aatatcatga acaaggaggc ttttatgaac
     1621 caggagatca cgaaagcctt ccacgtccgg aaagccaatc ggatcaaaga atgtctcagc
     1681 aagggcacct tctcagacgt gacatttacc ttggatgacg gagccatcag cgcccacaag
     1741 ccgctgctga tctgtagctg cgaatggatg gcggccatgt tcgggggttc ctttgtggaa
     1801 agcgccaacc gtgaggttca cctcccaaat ataaacaaga tgtcgatgca ggcggtgttg
     1861 gaatacctct acaccaagca gttgtcaccc aacttggacc tggaccccct ggagttaatt
     1921 gccttggcaa acagattttg cctgacacac ttggttgctc tcgtagagca gcatgctgtc
     1981 caggagctca ccaaggccgc tgtcagtggt gtgagcatcg atggtgaagt tctctcctac
     2041 ttggagctgg cacagtttca caatgccaat cagctagctg cctggtgttt gcatcacatc
     2101 tgcaccaact acaacagcgt ctgttccaag ttccgcaagg aaatcaaatc caagtcagca
     2161 gacaaccagg agtactttga gcggcaccgc tggccccctg tgtggtacct gaaggaggaa
     2221 gaccactacc agcgtgtgaa gagggaacga gagaaagagg acctggcgct gaacaagcac
     2281 cattcaagga gaaagtggtg tttctggcat tcgtccccag ctgtcgcctg aggaggagag
     2341 aggggaccgt cacccgctaa gggccacctc cactgttgac agctctacta taaaaaccag
     2401 tctccttagc catccgcaca gttgggtctg gggccctggc ttttccactc gtgtgcattt
     2461 acctccacca ggatcaaagc acaagccctg ccaccaatga gcagggacat aaaaggaaag
     2521 ctgactgact ctgaagtcct tcaaccagaa tgtccttttg gtttttttct ttcttggtta
     2581 ggaagctact acacttcagt ccgttacttt gttccttttg atacaaagaa aaaaaaacaa
     2641 aatccagctt tcgtgtctca gattccagat tggagaatat ttttctatgg actcttgtat
     2701 ttcactgagg tgacaatact gtgtattact ttattttata ggccacacat tgaccacgaa
     2761 gctgcccccc cccccgtgac tctttcagcc cacgtgacca ctgagcatca ctcataatta
     2821 cagagttatc ctttcgttgg gaggtaaagg gttgagctga agaggcctga agtattcaag
     2881 ctagaagcgc ttaatctttg ctctgatgag ccacgttggt ataattatgt atttacagga
     2941 tctccttttt aagtgctagt aattacatgg ttatccgtgc catatagcta cagtgagggc
     3001 aaatatccta ctgtttaaga ggtctagatt caaagaggac aatccttaca atcattacaa
     3061 gggcttattc caccccaatc ctatcagggg catgactgac tgccggaccc gggtccctac
     3121 aacgccattc gagtcctacc attggataac tatgtcaggg ttggtgactt aaaagtgctc
     3181 tgtggttttg ctgtgccttc accagcaaac cgtcctaggg cctgaacaga gataagggaa
     3241 agggaaaagg ctcatcccac tgtcttcagg tcaatcacct cggcctcaca cctgaggcca
     3301 acttcacttt gtaagacaag ttgttgttgt tgttgttgtt gttgttgttg ttgttctttt
     3361 tgttttttta actcttccca gggaaaattc aggtcaccac taaagtaggc caaggcagac
     3421 cacctgcttc tccactcggt ctcaccacag agcctgtgaa agcacaaagc taggggccag
     3481 gtcctgcctt ctccaagcag ggaaaggagc caaatcctaa gagtgtgggg aatggtgcta
     3541 ttatgtggta attctgaccc cgggtccctt gggaagatca gtgttagaac agaacttgca
     3601 aagcagggga gctctcaagc ggcatcaacg tcattgcctc tctgcagata aaatgttaca
     3661 gtataatata cacgtgtgtg cacacacaca cacacacaca cacaatcatc agagagtcac
     3721 agctccacac ctgccacggg atgtcgtgtg ctggacaccg gggcccattc tgttgagagg
     3781 tcaccttact gatgctaagg gctattgatt tttgtttgtt tgtttgtttg tttgtttttg
     3841 tttgggggag ggagtttctg aagacaggaa agagtcagtt gagtccaaat gccatatgga
     3901 tttgaaaagc tgtgtagacc acaggcaggc gttttgtttc tggcatggcc acataccaga
     3961 tacaagagct tagaaagacc caggaaggca tcaccctctt gtggcagaga gggagccgca
     4021 ggggaagggg ggcggggagg gggacagctt atcaagcctt cccccttaac tttgttaata
     4081 gggcaaatgg ctgacggaaa gagaaggtgg gtggaaggaa agagtacacg tcaagacttg
     4141 aggagaagga acatatagcc acctagaggt tctgttttca gtgagtaggc ctagttgaac
     4201 ttcaacaacc aaagctccat ccgcttgtac tcttgccaaa acacaacaga cacatacatg
     4261 aacatgggac gaaagcaata atatcctctc gtgttcttca cagctgctgg aaccaagtgg
     4321 ctggttcatt tggttgacac tgatccgcct ttaaccacga tggttcgttt tgtgtttgtt
     4381 tgttttttta atttcacaga aagcaaataa aattgtttag ctataaaaaa aaa
//
