LOCUS       NM_021375               1912 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus Rhesus blood group-associated B glycoprotein (Rhbg),
            mRNA.
ACCESSION   NM_021375
VERSION     NM_021375.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Mishra,S., Welch,N., Singh,S.S., Singh,K.D., Bellar,A., Kumar,A.,
            Deutz,L.N., Hanlon,M.D., Kant,S., Dastidar,S., Patel,H.,
            Agrawal,V., Attaway,A.H., Musich,R., Stark,G.R., Tedesco,F.S.,
            Truskey,G.A., Weiner,I.D., Karnik,S.S. and Dasarathy,S.
  TITLE     Ammonia transporter RhBG initiates downstream signaling and
            functional responses by activating NFkappaB
  JOURNAL   Proc Natl Acad Sci U S A 121 (31), e2314760121 (2024)
   PUBMED   39052834
  REMARK    GeneRIF: Ammonia transporter RhBG initiates downstream signaling
            and functional responses by activating NFkappaB.
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1912)
  AUTHORS   Abdulnour-Nakhoul,S., Le,T., Rabon,E., Hamm,L.L. and Nakhoul,N.L.
  TITLE     Structural determinants of NH3 and NH4+ transport by mouse Rhbg, a
            renal Rh glycoprotein
  JOURNAL   Am J Physiol Renal Physiol 311 (6), F1280-F1293 (2016)
   PUBMED   27681563
  REMARK    GeneRIF: Utilizing a structural homology analysis to AmtB, we have
            identified residues in Rhbg that are implicated in ion transport
            function. We introduced mutations into these residues and evaluated
            their function using measurements of intracellular pH (pHi),
            surface pH (pHs), and whole cell currents (I) that can distinguish
            between NH3 and NH4+ signals. Our results identified critical amino
            acids that affect transport.
REFERENCE   4  (bases 1 to 1912)
  AUTHORS   Caner,T., Abdulnour-Nakhoul,S., Brown,K., Islam,M.T., Hamm,L.L. and
            Nakhoul,N.L.
  TITLE     Mechanisms of ammonia and ammonium transport by rhesus-associated
            glycoproteins
  JOURNAL   Am J Physiol Cell Physiol 309 (11), C747-C758 (2015)
   PUBMED   26354748
  REMARK    GeneRIF: We characterized ammonia and ammonium (NH3/NH4 (+))
            transport by the rhesus-associated (Rh) glycoproteins RhAG, Rhbg,
            and Rhcg expressed in Xenopus oocytes. We used ion-selective
            microelectrodes and two-electrode voltage clamp to measure changes
            in intracellular pH, surface pH, and whole cell currents induced by
            NH3/NH4 (+) and methyl amine/ammonium (MA/MA(+)).
REFERENCE   5  (bases 1 to 1912)
  AUTHORS   Lee,H.W., Osis,G., Handlogten,M.E., Guo,H., Verlander,J.W. and
            Weiner,I.D.
  TITLE     Effect of dietary protein restriction on renal ammonia metabolism
  JOURNAL   Am J Physiol Renal Physiol 308 (12), F1463-F1473 (2015)
   PUBMED   25925252
REFERENCE   6  (bases 1 to 1912)
  AUTHORS   Weiner,I.D., Miller,R.T. and Verlander,J.W.
  TITLE     Localization of the ammonium transporters, Rh B glycoprotein and Rh
            C glycoprotein, in the mouse liver
  JOURNAL   Gastroenterology 124 (5), 1432-1440 (2003)
   PUBMED   12730882
  REMARK    GeneRIF: Basolateral RhBG is expressed by perivenous hepatocytes,
            where it may mediate ammonium uptake
REFERENCE   7  (bases 1 to 1912)
  AUTHORS   Quentin,F., Eladari,D., Cheval,L., Lopez,C., Goossens,D., Colin,Y.,
            Cartron,J.P., Paillard,M. and Chambrey,R.
  TITLE     RhBG and RhCG, the putative ammonia transporters, are expressed in
            the same cells in the distal nephron
  JOURNAL   J Am Soc Nephrol 14 (3), 545-554 (2003)
   PUBMED   12595489
  REMARK    GeneRIF: RhBG and RhCG are present in a major site of ammonia
            secretion in the kidney, i.e., the CNT and CD, in agreement with
            their putative role in ammonium transport.
REFERENCE   8  (bases 1 to 1912)
  AUTHORS   Verlander,J.W., Miller,R.T., Frank,A.E., Royaux,I.E., Kim,Y.H. and
            Weiner,I.D.
  TITLE     Localization of the ammonium transporter proteins RhBG and RhCG in
            mouse kidney
  JOURNAL   Am J Physiol Renal Physiol 284 (2), F323-F337 (2003)
   PUBMED   12388412
  REMARK    GeneRIF: Localization of the ammonium transporter proteins RhBG and
            RhCG in mouse kidney.
REFERENCE   9  (bases 1 to 1912)
  AUTHORS   Liu,Z., Peng,J., Mo,R., Hui,C. and Huang,C.H.
  TITLE     Rh type B glycoprotein is a new member of the Rh superfamily and a
            putative ammonia transporter in mammals
  JOURNAL   J Biol Chem 276 (2), 1424-1433 (2001)
   PUBMED   11024028
REFERENCE   10 (bases 1 to 1912)
  AUTHORS   Liu,Z., Chen,Y., Mo,R., Hui,C., Cheng,J.F., Mohandas,N. and
            Huang,C.H.
  TITLE     Characterization of human RhCG and mouse Rhcg as novel nonerythroid
            Rh glycoprotein homologues predominantly expressed in kidney and
            testis
  JOURNAL   J Biol Chem 275 (33), 25641-25651 (2000)
   PUBMED   10852913
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK081882.1.
            
            On Apr 6, 2007 this sequence version replaced NM_021375.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK081882.1, AF193808.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1912              AK081882.1         2-1913
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.78 cM"
     gene            1..1912
                     /gene="Rhbg"
                     /note="Rhesus blood group-associated B glycoprotein"
                     /db_xref="GeneID:58176"
                     /db_xref="MGI:MGI:1927379"
     exon            1..216
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..1406
                     /gene="Rhbg"
                     /note="Rh type B glycoprotein; rh family type B
                     glycoprotein; rhesus blood group family type B
                     glycoprotein"
                     /codon_start=1
                     /product="ammonium transporter Rh type B"
                     /protein_id="NP_067350.2"
                     /db_xref="CCDS:CCDS17466.1"
                     /db_xref="GeneID:58176"
                     /db_xref="MGI:MGI:1927379"
                     /translation="MARVPRHRRLVLPLLCLLFQGATALLFAIFVRYNHETDAALWHW
                     GNHSNVDNEFYFRYPSFQDVHVMVFVGFGFLMVFLQRYGFSSVGFTFLVASLTLQWAT
                     LLQGFLHSFHGGHIHVGVESLINADFCAGAVLISFGAVLGKTGPAQLLLMALLEAVLF
                     SVNEFILLSLLGVRDAGGSMTIHTFGAYFGLFLSWVLYRSQLEKSRHRQSSVYNSDLF
                     AMIGTIFLWVFWPSFNSAPTALGDGQHRTVVNTYYSLTASTLSTFALSALVSGDGRLD
                     MVHVQNAALAGGVVVGTSSEMMLTPFGALAAGFLAGTVSTLGYKFFTPILESRFKLQD
                     TCGVHNLHGMPGVLGAILGVVVAALATHEAYGDGLQSVFPLIAKGQRSATSQAVYQLF
                     GMFVTLVFASVGGSLGGLLLRLPFLDSPPDSQCFEDQVYWEVPGEQETETQRPLRGGE
                     SDTRA"
     exon            217..403
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            404..554
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            555..702
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            703..869
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1007
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1141
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1263
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1337
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1912
                     /gene="Rhbg"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1634..1639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhbg"
                     /note="hexamer: GATAAA"
     polyA_site      1662
                     /gene="Rhbg"
                     /note="major polyA site"
ORIGIN      
        1 actgccctgc cggagaccgg gagaacacag ccgcgttcat ggcccgcgtt ccccgccacc
       61 ggcgcctggt cctgccgctg ctgtgcctgc tcttccaagg cgccactgcc ctcctctttg
      121 ctatctttgt ccggtacaac cacgaaaccg acgctgccct gtggcactgg ggcaaccaca
      181 gtaacgtgga caacgagttt tactttcgct acccaagctt ccaggatgtg cacgtcatgg
      241 tcttcgtggg ctttggcttc ctcatggtct ttctacagcg gtacggcttc agcagtgtgg
      301 gctttacctt cctcgtggcc agcctcaccc tgcagtgggc cacactgctc caaggcttcc
      361 tccactcttt ccacggtggc cacatccatg ttggcgtgga gagtttgatc aacgctgact
      421 tctgcgcggg agctgtgctc atctctttcg gggctgttct gggcaagact gggccagccc
      481 agctgctgct aatggctcta ctggaggcag tgctgtttag cgtcaacgag tttatactac
      541 tcagtctcct gggggtgaga gatgctggag ggtccatgac aattcacaca tttggggcct
      601 acttcgggct gttcctctca tgggtcctct acagatccca gctggagaag agcaggcatc
      661 gccagagctc tgtctacaac tctgacctct ttgccatgat cgggaccatc ttcctgtggg
      721 ttttctggcc cagcttcaac tccgcgccga cagcgctggg ggatgggcag catcggaccg
      781 tggtcaacac atactattca ctcaccgcaa gcaccctcag tactttcgcc ttgtcagccc
      841 ttgtcagtgg agatggacga ctggacatgg tccacgtcca gaacgcagca ctggctggag
      901 gtgttgtggt ggggacatca agtgagatga tgctgacacc ctttggggcg ctggcagctg
      961 gcttcctggc tgggactgtc tccacactgg ggtataagtt ctttacgcct atccttgaat
     1021 ccagatttaa actgcaagac acatgtggtg ttcacaacct ccatgggatg ccaggggtcc
     1081 tgggggccat cctgggagtc gtagtggctg cactggccac ccacgaagct tatggagatg
     1141 gcctgcagag tgtgtttcca ctcatagcca agggtcagcg gagtgccacg tctcaggccg
     1201 tgtatcagct cttcggaatg tttgtcacac tggtgtttgc ctctgtgggt ggcagccttg
     1261 gagggctcct gctgcggctc ccctttctgg actcccctcc agactcccaa tgcttcgagg
     1321 accaggttta ctgggaggta cctggagagc aggagactga gacccagagg cctctgaggg
     1381 gtggggagtc agacacccgg gcctaattgc tgccagcgtg agactgttct cagagaagac
     1441 aatggctcct gccaagagag ccttctctgc ctttgttact gttgtgcctg gaagcctgcc
     1501 gcccctaggc agatccccat tagctgtggc cagaaaaggc caaagccagc ctaggtgact
     1561 gccaccccat tgaccagtgt tactctgttc cagtgtcacc caagccagct ccactctggg
     1621 tgggaagctg ggtgataaac aatgtatcac caaggactct gacacctgtg tgcgcctctc
     1681 cctttcactg ctgaggccaa aatcttgtga caatcaaaga ccttagtgtt gacaaaccct
     1741 ctttgggtcc acagaactcc acattctact gtatatgtcc caactagtcc catcctctcc
     1801 agggagaggc attattgtcc agaatgtctg tgtccacttc agccatctga tgctgtcccc
     1861 aaatccttcc ctcagctggc tttccagtgc tgggaagaat aggaagaagc cc
//
