LOCUS       NM_183163               1539 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus rhomboid like 2 (Rhbdl2), transcript variant 1, mRNA.
ACCESSION   NM_183163
VERSION     NM_183163.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Yorgan,T.A., Peters,S., Amling,M. and Schinke,T.
  TITLE     Osteoblast-specific expression of Panx3 is dispensable for
            postnatal bone remodeling
  JOURNAL   Bone 127, 155-163 (2019)
   PUBMED   31202927
REFERENCE   3  (bases 1 to 1539)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1539)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1539)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1539)
  AUTHORS   Cheng,T.L., Wu,Y.T., Lin,H.Y., Hsu,F.C., Liu,S.K., Chang,B.I.,
            Chen,W.S., Lai,C.H., Shi,G.Y. and Wu,H.L.
  TITLE     Functions of rhomboid family protease RHBDL2 and thrombomodulin in
            wound healing
  JOURNAL   J Invest Dermatol 131 (12), 2486-2494 (2011)
   PUBMED   21833011
  REMARK    GeneRIF: RHBDL2 and soluble thrombomodulin were upregulated in ex
            vivo tissue culture of injured mouse skin. 3,4-Dichloroisocoumarin
            inhibited thrombomodulin production and wound healing; this was
            reversed by recombinant thrombomodulin in mice.
REFERENCE   7  (bases 1 to 1539)
  AUTHORS   Zettl,M., Adrain,C., Strisovsky,K., Lastun,V. and Freeman,M.
  TITLE     Rhomboid family pseudoproteases use the ER quality control
            machinery to regulate intercellular signaling
  JOURNAL   Cell 145 (1), 79-91 (2011)
   PUBMED   21439629
REFERENCE   8  (bases 1 to 1539)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683811.6.
            
            On Feb 7, 2022 this sequence version replaced NM_183163.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2521014.1,
                                           SRR5189685.260132.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AL683811.6         67152-67274
            124-386             AL683811.6         89217-89479
            387-535             AL683811.6         93546-93694
            536-648             AL683811.6         97167-97279
            649-749             AL683811.6         102060-102160
            750-810             AL683811.6         104164-104224
            811-872             AL683811.6         106141-106202
            873-1539            AL683811.6         108837-109503
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 57.42 cM"
     gene            1..1539
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /note="rhomboid like 2"
                     /db_xref="GeneID:230726"
                     /db_xref="MGI:MGI:3608413"
     exon            1..123
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            124..386
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1052
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /EC_number="3.4.21.105"
                     /note="isoform 1 is encoded by transcript variant 1;
                     rhomboid-related protein 2; rhomboid, veinlet-like 2"
                     /codon_start=1
                     /product="rhomboid-related protein 2 isoform 1"
                     /protein_id="NP_898986.2"
                     /db_xref="CCDS:CCDS38873.1"
                     /db_xref="GeneID:230726"
                     /db_xref="MGI:MGI:3608413"
                     /translation="MAVAHEMEMESVNLNMEREGKEEPEEEKMKGNGEGKDFPRSRKV
                     HRIVSKWMLPEPVRRTYLERANCLPPPLFIILISLAELAVFIYYAVWKPQKQWITLDT
                     GILESPLTYCPEKREEAWRFISYMLVHAGVQHIVGNLLMQIVLGIPLEMVHKGLRVGL
                     VYLAGVLAGSLASSIFDPLKSLVGASGGVYALMGGYFMNVIVNFREMIPAFGIVRLLV
                     IILIVASDMGFALYRRFFVPANGSPVSFAAHIAGGFAGMSIGYTVFSCFDKTLLKDPR
                     FWIAIAAYVACLLFAVFFNIFLSPAN"
     exon            387..535
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..648
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..749
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            750..810
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            811..872
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1539
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1517..1522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /note="hexamer: AATAAA"
     polyA_site      1539
                     /gene="Rhbdl2"
                     /gene_synonym="9130416B15; RRP2"
                     /note="major polyA site"
ORIGIN      
        1 agttctggga gggcgggagg agagcctggc aggctgcggc tcgggcctcc aggcagctcc
       61 gttggctgct tgaagtgcaa gatccgactg cagtgtggga ccactgtgcc tcggcctccc
      121 caggcggagg gaggacccag acaatggctg ttgctcacga gatggagatg gagagtgtga
      181 atctgaacat ggagagagag ggaaaggaag aaccggagga ggagaaaatg aaagggaatg
      241 gagaaggtaa agacttcccc aggagcagaa aggtccacag gatcgtctcc aagtggatgc
      301 ttcctgagcc agtccgaaga acatacttag agagagccaa ctgcctcccg ccgcccctgt
      361 tcatcatcct catcagtctg gctgagctgg ctgtgtttat ttattatgcg gtgtggaagc
      421 cacagaagca gtggatcacc ctggacacgg ggatcttgga aagtcccttg acgtactgcc
      481 ctgagaagag ggaggaagcc tggaggttta tctcatacat gctggtccat gccggagtcc
      541 agcacatcgt ggggaatctt cttatgcaga tcgtcctggg tattcctttg gaaatggtcc
      601 acaaaggcct ccgagtgggg ctggtgtacc tggcaggagt gcttgcaggt tccctggcca
      661 gctccatctt tgaccccctc aagtctcttg tgggagcttc aggaggagtc tatgcattga
      721 tgggaggata ttttatgaat gttatagtga actttcgaga aatgattcct gcctttggaa
      781 tcgtcagact actcgttatc attctgatag ttgcgtcaga catgggattt gctctctaca
      841 gaagattctt tgtccctgca aatgggtctc cggtgtcctt tgctgctcac attgcaggtg
      901 gatttgctgg gatgtccata ggttacactg tgttcagctg ctttgacaag accctgctca
      961 aagaccccag gttctggatc gcaattgctg catatgtagc ttgtctctta tttgctgtgt
     1021 tttttaacat ctttctgtcc ccagcaaact gacttgcctc tatgataaac cagttaatat
     1081 aaaagtgtca catgggacca ggtgtggtgg cacacgcctt taatcccagc actcgggagg
     1141 cagaggcagg cggatttctg agcttgaggc cagcctggtc tacaaagtga gttccaggtc
     1201 agccagggct acacagaaaa accctgtctc aaaacccccc cccaaaaaaa aaaccaaaaa
     1261 aaaaaaaagt gtcaccgggg gaggggaatt gtagactctg taaagaaaca cagatattcc
     1321 agcaaatgct aagaacatta taaagaaggc aggacagcga ctgagcttac aataggaggc
     1381 aagaagataa gaagcaaaga ccaggtgttt catgcacaga gcatgtgtgt attaccctgt
     1441 gaaggagact tgatatcctc gggggatgta acttggaggt tgtttatagg ctccatgaga
     1501 actgtactat cctgataata aacatttttt tttcacatc
//
