LOCUS       NM_011269               2316 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rhesus blood group-associated A glycoprotein (Rhag),
            mRNA.
ACCESSION   NM_011269
VERSION     NM_011269.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Al-Samir,S., Goossens,D., Cartron,J.P., Nielsen,S., Scherbarth,F.,
            Steinlechner,S., Gros,G. and Endeward,V.
  TITLE     Maximal Oxygen Consumption Is Reduced in Aquaporin-1 Knockout Mice
  JOURNAL   Front Physiol 7, 347 (2016)
   PUBMED   27559317
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2316)
  AUTHORS   Goossens,D., Trinh-Trang-Tan,M.M., Debbia,M., Ripoche,P.,
            Vilela-Lamego,C., Louache,F., Vainchenker,W., Colin,Y. and
            Cartron,J.P.
  TITLE     Generation and characterisation of Rhd and Rhag null mice
  JOURNAL   Br J Haematol 148 (1), 161-172 (2010)
   PUBMED   19807729
REFERENCE   4  (bases 1 to 2316)
  AUTHORS   Ripoche,P., Goossens,D., Devuyst,O., Gane,P., Colin,Y.,
            Verkman,A.S. and Cartron,J.P.
  TITLE     Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell
            ghosts: a stopped-flow analysis
  JOURNAL   Transfus Clin Biol 13 (1-2), 117-122 (2006)
   PUBMED   16574458
  REMARK    GeneRIF: Review. Rhag plays a major role in the NH3 conductance of
            erythrocytes, but probably not in CO2 transport.
            Review article
REFERENCE   5  (bases 1 to 2316)
  AUTHORS   Bakouh,N., Benjelloun,F., Cherif-Zahar,B. and Planelles,G.
  TITLE     The challenge of understanding ammonium homeostasis and the role of
            the Rh glycoproteins
  JOURNAL   Transfus Clin Biol 13 (1-2), 139-146 (2006)
   PUBMED   16564724
  REMARK    GeneRIF: RhAG expression enhanced the ammonium-induced initial
            alkalinization (related to NH3 influx)& secondary acidification
            (related to NH4+ influx). Sub-millimolar NH4+ concentrations
            induced inward currents in voltage-clamped RhAG-expressing cells.
            Review article
REFERENCE   6  (bases 1 to 2316)
  AUTHORS   Kitano,T. and Saitou,N.
  TITLE     Evolutionary history of the Rh blood group-related genes in
            vertebrates
  JOURNAL   Immunogenetics 51 (10), 856-862 (2000)
   PUBMED   10970100
REFERENCE   7  (bases 1 to 2316)
  AUTHORS   Huang,C.H., Liu,Z., Apoil,P.A. and Blancher,A.
  TITLE     Sequence, organization, and evolution of Rh50 glycoprotein genes in
            nonhuman primates
  JOURNAL   J Mol Evol 51 (1), 76-87 (2000)
   PUBMED   10903374
REFERENCE   8  (bases 1 to 2316)
  AUTHORS   Liu,Z. and Huang,C.H.
  TITLE     The mouse Rhl1 and Rhag genes: sequence, organization, expression,
            and chromosomal mapping
  JOURNAL   Biochem Genet 37 (3-4), 119-138 (1999)
   PUBMED   10495887
REFERENCE   9  (bases 1 to 2316)
  AUTHORS   Matassi,G., Cherif-Zahar,B., Pesole,G., Raynal,V. and Cartron,J.P.
  TITLE     The members of the RH gene family (RH50 and RH30) followed
            different evolutionary pathways
  JOURNAL   J Mol Evol 48 (2), 151-159 (1999)
   PUBMED   9929383
REFERENCE   10 (bases 1 to 2316)
  AUTHORS   Kitano,T., Sumiyama,K., Shiroishi,T. and Saitou,N.
  TITLE     Conserved evolution of the Rh50 gene compared to its homologous Rh
            blood group gene
  JOURNAL   Biochem Biophys Res Commun 249 (1), 78-85 (1998)
   PUBMED   9705835
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121932.2.
            
            On Nov 17, 2006 this sequence version replaced NM_011269.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF057526.1, AK143851.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-214               AC121932.2         141973-142186       c
            215-398             AC121932.2         124789-124972       c
            399-549             AC121932.2         124435-124585       c
            550-697             AC121932.2         121998-122145       c
            698-864             AC121932.2         121593-121759       c
            865-1002            AC121932.2         119789-119926       c
            1003-1124           AC121932.2         118581-118702       c
            1125-1195           AC121932.2         117248-117318       c
            1196-1269           AC121932.2         116845-116918       c
            1270-2316           AC121932.2         112581-113627       c
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 19.54 cM"
     gene            1..2316
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /note="Rhesus blood group-associated A glycoprotein"
                     /db_xref="GeneID:19743"
                     /db_xref="MGI:MGI:1202713"
     exon            1..214
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1344
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /note="50 kD glycoprotein; rh family type A glycoprotein;
                     erythrocyte membrane glycoprotein Rh50; rhesus blood group
                     family type A glycoprotein"
                     /codon_start=1
                     /product="ammonium transporter Rh type A"
                     /protein_id="NP_035399.1"
                     /db_xref="CCDS:CCDS28787.1"
                     /db_xref="GeneID:19743"
                     /db_xref="MGI:MGI:1202713"
                     /translation="MRFKFPLMAISLEVAMIVLFGLFVEYETPQNASQKNASHQNASQ
                     QGNTSSSAKKDQFFQLYPLFQDVHVMIFVGFGFLMTFLKKYGFSGVGFNLFLAALGLQ
                     WGTIMQGLLHSHGKEFHFGIYNMINADFSTATVLISFGAVLGKTSPIQMLIMTILEIA
                     VFAGNEYLVTELFEASDTGASMTIHAFGAYFGLAVAGVLYRPGLRCEHPNDESVYHSD
                     LFAMIGTLFLWIFWPSFNSAIADPGDHQYRAIVNTYMSLAACVITAYALSSLVERRGR
                     LDMVHIQNATLAGGVAVGTCADMEIPLYAAMTIGSIAGIISVLGYKFFSPLLANKLMI
                     HDTCGVHNLHGLPGVFGGLASIVAISWGMSTASMAMQAAALGSSIGSAIVGGLLTGLI
                     LKLPIWNQPPDEYCYDDSVSWKVPKFRELDNRFFQHANHNHVEHEV"
     exon            215..398
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            399..549
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            550..697
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            698..864
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1002
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1124
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1195
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1269
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..2316
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2294..2299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /note="hexamer: ATTAAA"
     polyA_site      2314
                     /gene="Rhag"
                     /gene_synonym="CD241; Rh50; Rh50A"
                     /note="major polyA site"
ORIGIN      
        1 attgcacctc tgtcctctgc tgcagtcatg aggttcaaat tccctctcat ggccataagc
       61 ctggaagttg ctatgattgt tttatttgga ttatttgttg aatatgagac accacagaat
      121 gcttcccaga agaatgcttc ccaccagaat gcttcccagc agggtaacac ctcaagttca
      181 gcaaaaaagg accaattttt tcagttgtat cccttattcc aagatgtgca tgtcatgata
      241 tttgttgggt ttggcttcct catgaccttt ctgaagaaat atggtttcag tggtgtgggt
      301 ttcaacctct ttcttgctgc tctgggcctc caatggggta cgattatgca gggccttctt
      361 cacagccacg gaaaggaatt tcacttcgga atctacaata tgataaatgc agacttcagc
      421 acagccacag ttctcatttc ctttggcgct gtcctgggga aaacaagccc cattcaaatg
      481 ttgatcatga caattctgga aattgctgta tttgctggca acgaatatct tgttactgaa
      541 ttatttgagg catctgacac tggagcatca atgacaatcc atgcctttgg agcttacttt
      601 ggcttagcag tggcaggtgt gttatatcgg cctggactca gatgtgaaca cccaaatgat
      661 gaatctgtgt accactctga cttgtttgcc atgatcggaa cacttttcct gtggatattt
      721 tggcccagct ttaattcagc cattgctgat cctggagatc atcagtatag ggccattgtc
      781 aacacataca tgtcccttgc agcctgtgtg atcacagcct atgccttgtc cagccttgta
      841 gagcgccgag gcaggctgga tatggtacac attcagaatg ctactctagc aggaggtgtt
      901 gctgtgggca catgtgcaga catggaaatc cccctatatg ctgctatgac cattggaagc
      961 attgcaggga tcatctctgt gcttggatac aagttcttta gtccactgtt agctaataaa
     1021 ctgatgatcc atgatacatg tggggttcat aacttgcatg gcttacctgg agtttttgga
     1081 ggccttgcca gcattgtggc cataagctgg gggatgtcta ctgcgtctat ggctatgcag
     1141 gcagcagcac tgggatcttc cattggctca gcgattgttg gaggtttgct tacaggtcta
     1201 attctgaagt tgcctatctg gaaccagcca cctgatgaat actgctatga tgactctgtt
     1261 tcttggaagg ttcccaaatt cagagaactt gataatcgct tctttcaaca tgcaaatcac
     1321 aaccacgtgg aacatgaagt ctaaaagctg atcctaaact ctgcatcctc ggaattgcca
     1381 agaagagtca aatttaaaga aacaaaaact tggtatccaa agagataggc atgacaatag
     1441 ataaattcca gccccaaatg gccagtgtac aaatgtgttt agttttacta ttattccatg
     1501 ctgtccaaat gatttctcag ttgacaggaa aatgctcaga aagccgtcta tcccaggctc
     1561 taagaatgaa gttgcaaatg gatagcttca tcgagaatgt ttgattgtat cattgatatc
     1621 ttgcaaaaga gatgaattat agaaatcaat acttcttgat cacatcagac tttatccact
     1681 tttttggtta gtttacatct ttgtgcatgt gtgtcatacc ccttccccta ccctctgcac
     1741 atccctaccc actgcagttc ctcatgattc cccactccct ccatctgggt cccttcctcc
     1801 cctcagttag gtattccttg ttttattaca tgtattctat ccgcaaccct ttagcttcct
     1861 aatgtacata ccatgtcttc agttccaaag agtttaaagc tgaagtcaaa ggacatatta
     1921 aagagtctat actagaaaaa tgagccgggt ggtggtggtg cacaccttta atcccagctc
     1981 ttgggaggca gaggcaggcg gatttctgag tgcgaggcca gcctggtcta taaagtgagt
     2041 tccaggacag ccagggatat acagaaaaac cctgtctcaa aaatagcccc ccccccaaaa
     2101 aaaagaaaaa gaaaaagaaa tctatgtaaa taaaaagcaa tattaatgtt ctagttagca
     2161 tctttttaaa tcttaaaaac agtcatagta atgttatact gttgtttgtt tgcatattct
     2221 tatccttaat ttctttttat ggttcttatt tgtaatagga gtttaaaagc catctgttat
     2281 aaaattttaa atgattaaat gttatataaa atgggg
//
