LOCUS       NM_133231               2185 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus regulatory factor X-associated protein (Rfxap), mRNA.
ACCESSION   NM_133231
VERSION     NM_133231.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   3  (bases 1 to 2185)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   4  (bases 1 to 2185)
  AUTHORS   Peretti,M., Villard,J., Barras,E., Zufferey,M. and Reith,W.
  TITLE     Expression of the three human major histocompatibility complex
            class II isotypes exhibits a differential dependence on the
            transcription factor RFXAP
  JOURNAL   Mol Cell Biol 21 (17), 5699-5709 (2001)
   PUBMED   11486010
  REMARK    GeneRIF: Invitro transcription/translation and transfection studies
            described here show that non-AUG (ACG) codon functions as a
            translation initiation codon.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087489.1, AF335512.1, AW826111.1, BX519947.1, BQ830204.1 and
            AI607302.1.
            
            On Mar 22, 2007 this sequence version replaced NM_133231.1.
            
            CCDS Note: This CCDS representation uses a non-canonical ACG
            translation initiation codon. The use of this start codon is
            supported by experimental evidence in PMID:11486010. This start
            codon is conserved in several non-primate vertebrate species, while
            most primates have a canonical AUG start codon in the equivalent
            position.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK087489.1, SRR1660819.59791.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 11486010
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-392               AK087489.1         2-393
            393-992             AF335512.1         373-972
            993-1083            AW826111.1         1-91                c
            1084-1223           BX519947.1         401-540
            1224-1286           BQ830204.1         483-545
            1287-2152           AK087489.1         1288-2153
            2153-2185           AI607302.1         1-33                c
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 25.69 cM"
     gene            1..2185
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /note="regulatory factor X-associated protein"
                     /db_xref="GeneID:170767"
                     /db_xref="MGI:MGI:2180854"
     exon            1..593
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..812
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /exception="alternative start codon"
                     /note="non-AUG (ACG) translation initiation codon;
                     RFX-associated protein"
                     /codon_start=1
                     /transl_except=(pos:117..119,aa:Met)
                     /product="regulatory factor X-associated protein"
                     /protein_id="NP_573494.1"
                     /db_xref="CCDS:CCDS50907.1"
                     /db_xref="GeneID:170767"
                     /db_xref="MGI:MGI:2180854"
                     /translation="MEAQAVPEGSGPSTASPRTAPPVTVLVMRQDEAEADGALRPGLA
                     GSEAAADAEDEAGDDDADLLDTSDPAGGGESAASPEELEDEDAEGGGAARRRGSKTCT
                     YEGCRETTSQVAKQRKPWMCKKHRNKMYKDKYKKKKSDQALGSGGPSAASTGNVKLEE
                     STDNILSIVKQRTGSFGDRPARPTLLEQVLNQKRLSLLRSPEVVQFLQKQQQLLNQQV
                     LEQRQQHFPGAPV"
     exon            594..701
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            702..2162
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1059..1064
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1084
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /note="major polyA site"
     regulatory      2137..2142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2162
                     /gene="Rfxap"
                     /gene_synonym="5730495K23Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtgcagtgc tggcggttcg gctccgggac gtgcggcggg ctgccgcttg tcgagtacgg
       61 ttgtgtttct caagagctga cgggccgcgg tggcggaccc gggctcgggc ggcagcacgg
      121 aggcgcaggc tgtgcccgag ggctcggggc cgagcaccgc cagtccccgg acggcccctc
      181 cggtgacggt gctggtgatg cggcaggacg aggccgaggc ggacggcgct ctgaggccgg
      241 gcctggcggg gagtgaggcg gcggcagacg ccgaggacga ggcgggggac gacgacgccg
      301 acctgctgga cacatcggac ccggcgggcg gcggcgagag tgcggccagc ccggaggagc
      361 tggaggacga ggacgcggag ggtggcggcg cagcgcgcag gcgaggctcc aagacctgca
      421 cgtacgaggg ctgccgcgag accaccagcc aggtggccaa gcagcgcaag ccctggatgt
      481 gcaagaagca ccgcaacaag atgtacaagg acaagtacaa gaagaagaag agcgaccagg
      541 cactgggctc tggcggcccg tccgcggcca gcacaggcaa cgtcaaactg gaggaaagca
      601 cagacaacat actctccatt gtcaaacaga gaacaggatc ctttggtgat cgccctgcaa
      661 gacctactct tctagagcaa gtgttaaatc agaaaagact gtcattacta agaagtccag
      721 aagttgtcca gtttttacag aagcagcagc agctactaaa tcagcaagtt ttggagcaaa
      781 gacagcagca tttcccagga gcgccagtgt gaggcttacg gaggcttctg cgcgctacct
      841 tactgcgatg catgggtgct ttcagcctaa gacagtgctg gtaatcagac attgggggca
      901 cacctggaac ccctgaactt ggaaaactga gccaagagga tcccaagttc aaagccagcc
      961 tgggctacat agcaagttcc tgtctcaaaa gagagagcga gagcgagaga gagagagaga
     1021 aactactttc tgtaaatgtg tgtgtacatt tgagaataaa taaatattga atttttgcat
     1081 ttaattgttt catatcactg tgagtttgga aaaaaacacc tttatagtgt ttactgtttt
     1141 tcttcagtta tgcctactga agcgaatgag ccagttgctt tctgtggaag tcataaagta
     1201 acagtaatat caatcaccca gggctgcgat tctcaccaag aggccctgga aaggactggt
     1261 atgttttggg ctgccacagt gaccttgacc tagcgaggct gtagtgaggg tcagaaatcg
     1321 taaccttctg cagtctgtgg tattccacca gctaaatgtt cccttgccgt cagtgcgtct
     1381 ccgctgcatc gataacctgc ggccagctca aggtcaggtg cttggagtaa cctttctgtg
     1441 tagttcaggt ggagctctga aagctaagca tctttatacc tgtgaggaaa gtggtcactg
     1501 atctgtctct tcatgtaact ggagaaagag aggacactaa agttggtgcc tccagggttg
     1561 ggggtcactc tcctttaaag caggttgtct cctcagttga ttttgtgaac agttcagatg
     1621 tactcagccc accaaggcca ggacagggtt ggtttgctta tagtcattct ccataaagga
     1681 gaccctgaag gtagaatagc tctcactgta gccagtgctt ttcaaaactg agcacttatt
     1741 cctagctact gttaaaggaa cggaggatta tctgggtact ttttttaaaa aactttttcc
     1801 taggaaaact ttaaaaaagc aaaaatgcca taagatacag aatcaaatgt caagagagta
     1861 tatccaggag taagttaata agtgattcaa ggtaaaatgg atcagttggt tgattcctaa
     1921 actgaaatat atctgatgtt aaactcgtag ctacgataag tactcacagg gctacggatg
     1981 cagcttagcc ttactgcacc tgcctagcat gtgcaaggcc ctcagcccac tccccaccct
     2041 taccaccaca agaaacaaat acaaagtaga gtgattttct tcattttgtg ttctatactt
     2101 gtgtttctga aaaagaccct tttgccaaaa ttattcatta aaggacatcc gttgaacttt
     2161 gaaaaaaaaa aaaaaaaaaa aaaaa
//
