LOCUS       NM_019437               2482 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus riboflavin kinase (Rfk), mRNA.
ACCESSION   NM_019437
VERSION     NM_019437.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Hirano,A., Braas,D., Fu,Y.H. and Ptacek,L.J.
  TITLE     FAD Regulates CRYPTOCHROME Protein Stability and Circadian Clock in
            Mice
  JOURNAL   Cell Rep 19 (2), 255-266 (2017)
   PUBMED   28402850
  REMARK    GeneRIF: Knockdown of Rfk combined with a riboflavin-deficient diet
            altered the CRY levels in mouse liver and the expression profiles
            of clock and clock-controlled genes (especially those related to
            metabolism including glucose homeostasis).
REFERENCE   3  (bases 1 to 2482)
  AUTHORS   Schramm,M., Wiegmann,K., Schramm,S., Gluschko,A., Herb,M.,
            Utermohlen,O. and Kronke,M.
  TITLE     Riboflavin (vitamin B2) deficiency impairs NADPH oxidase 2 (Nox2)
            priming and defense against Listeria monocytogenes
  JOURNAL   Eur J Immunol 44 (3), 728-741 (2014)
   PUBMED   24272050
REFERENCE   4  (bases 1 to 2482)
  AUTHORS   Yazdanpanah,B., Wiegmann,K., Tchikov,V., Krut,O., Pongratz,C.,
            Schramm,M., Kleinridders,A., Wunderlich,T., Kashkar,H.,
            Utermohlen,O., Bruning,J.C., Schutze,S. and Kronke,M.
  TITLE     Riboflavin kinase couples TNF receptor 1 to NADPH oxidase
  JOURNAL   Nature 460 (7259), 1159-1163 (2009)
   PUBMED   19641494
  REMARK    GeneRIF: results suggest that TNF, through the activation of RFK,
            enhances the incorporation of FAD in NADPH oxidase enzymes, a
            critical step for the assembly and activation of NADPH oxidase
REFERENCE   5  (bases 1 to 2482)
  AUTHORS   Kenney-Hunt,J.P., Vaughn,T.T., Pletscher,L.S., Peripato,A.,
            Routman,E., Cothran,K., Durand,D., Norgard,E., Perel,C. and
            Cheverud,J.M.
  TITLE     Quantitative trait loci for body size components in mice
  JOURNAL   Mamm Genome 17 (6), 526-537 (2006)
   PUBMED   16783635
REFERENCE   6  (bases 1 to 2482)
  AUTHORS   Kneissl,M., Putter,V., Szalay,A.A. and Grummt,F.
  TITLE     Interaction and assembly of murine pre-replicative complex proteins
            in yeast and mouse cells
  JOURNAL   J Mol Biol 327 (1), 111-128 (2003)
   PUBMED   12614612
REFERENCE   7  (bases 1 to 2482)
  AUTHORS   Bauer,S., Kemter,K., Bacher,A., Huber,R., Fischer,M. and
            Steinbacher,S.
  TITLE     Crystal structure of Schizosaccharomyces pombe riboflavin kinase
            reveals a novel ATP and riboflavin-binding fold
  JOURNAL   J Mol Biol 326 (5), 1463-1473 (2003)
   PUBMED   12595258
REFERENCE   8  (bases 1 to 2482)
  AUTHORS   Merrill,A.H. Jr. and McCormick,D.B.
  TITLE     Preparation of flavin 5'-phosphates using immobilized flavokinase
  JOURNAL   Methods Enzymol 66, 287-290 (1980)
   PUBMED   6246391
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147369.3, AK002806.1, BB758770.1 and AV285208.1.
            
            On Oct 28, 2009 this sequence version replaced NM_019437.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051021.1, AK002806.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AC147369.3         94044-94083
            41-2251             AK002806.1         2-2212
            2252-2468           BB758770.1         310-526
            2469-2482           AV285208.1         253-266
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 12.82 cM"
     gene            1..2482
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /note="riboflavin kinase"
                     /db_xref="GeneID:54391"
                     /db_xref="MGI:MGI:1914688"
     exon            1..350
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..736
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /EC_number="2.7.1.26"
                     /note="flavokinase; ATP:riboflavin 5'-phosphotransferase"
                     /codon_start=1
                     /product="riboflavin kinase"
                     /protein_id="NP_062310.1"
                     /db_xref="CCDS:CCDS29688.1"
                     /db_xref="GeneID:54391"
                     /db_xref="MGI:MGI:1914688"
                     /translation="MRSLPFFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADVST
                     GIYYGWASVGSGDVHKMVVSIGWNPYYKNVKKSMETHIIHTFKEDFYGEILNVAIVGY
                     LRPEKNFDSLESLISAIQGDIEEAKKQLDLPEHLKLKDDNFFQVSKGKIMNGH"
     exon            351..502
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /inference="alignment:Splign:2.1.0"
     exon            503..605
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /inference="alignment:Splign:2.1.0"
     exon            606..2482
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1585..1590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /note="hexamer: AATAAA"
     polyA_site      1606
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
     regulatory      2444..2449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /note="hexamer: AATAAA"
     polyA_site      2470
                     /gene="Rfk"
                     /gene_synonym="0610038L10Rik; KOI-4"
                     /note="major polyA site"
ORIGIN      
        1 gggagagcaa cggaagccga gcaggccggt cgcctgggcc gcagacctgt gcattcgccg
       61 cggttgtcgt agttccggcc gagcggccgc tgggatctga cggctgggtg ctatcctgcg
      121 ggcaggcact ggtcgcggca ggtgtctcgc tccgccggcc cagctcccgc gggatccccc
      181 ggctggtccg ggccccggcg ccgcactcac ggcggctccg tcgcaggcac ggagcgagga
      241 gcagcccgcg tcctccgagg tcagcgtcat gaggagcctg ccgttcttct gccgcggcca
      301 ggtggtgcgt ggcttcggcc gcggctccaa gcaactgggc atccccacag ccaattttcc
      361 tgaacaagtg gtggacaatc ttccagctga tgtgtctact ggcatttatt atggttgggc
      421 cagcgttggg agcggcgatg tccataaaat ggtggtgagc attggatgga acccgtacta
      481 taagaatgtg aaaaagtcca tggaaacaca catcatccat accttcaaag aggacttcta
      541 tggggaaatt ctcaatgtgg ccattgttgg ctatctcaga cctgaaaaga actttgattc
      601 tttagagtca cttatttctg caattcaagg tgatattgaa gaagctaaaa agcaactgga
      661 tttacccgaa catttgaaac tcaaagatga taatttcttc caagtttcta aaggcaaaat
      721 tatgaatggc cactaatgaa aagtccctta cctgcctacc actagtgtca cgcatagtgt
      781 cacagtgctt ctgatacact ctcatcttga tcatatttta aatcaagcgt tttcctgtag
      841 ctgtcaagtg gtctccagca gttcactcca tcatgttgca cagtactaag agactcattc
      901 agaaatcaag actttagatg taatatgttg attaaaatgt agcactagaa agcttgtact
      961 gaaaataaag tctgtgagtg tgggtggaaa agcaagttcc tgaggaaaat gaggactgac
     1021 catagttctt gtgactgagt gtgctggcac tggtgagccc agccagcctg tgctgaacag
     1081 aggcttttca ggaagtgcac cacacacaca cacacacaca cacacacaca cagagcttac
     1141 ccatgaagga aaaattagag ttacttttct cattttgtgc attgatatat ttttacaatc
     1201 ctgcaaactt ccttgagtca ctcacgactc cccctgaggt cacttcctgg tagtgtccca
     1261 atgctctgtg tgcagttact tcaggttgaa gttgccttga tcgcctgccc ctcatcaggg
     1321 cttttgcttt tcttcaaagt actgaggatc caacccagtg ccttacacgc caggcaagtg
     1381 tgaccactga gctaggctcc cgcccccttt actttttgaa gccatacaat ggatgtctat
     1441 atgttgcctt tttgaagatt aggtggtttt gctctttaac atttttgtga ctttggttta
     1501 ttttcttccc atgtcgccca gagattactt gtgtgtgtgc cattctgctc aaaactaaat
     1561 ggtggtcagg ttcaagtaaa gaaaaataaa acaagctgca tttatagact tgcttggaat
     1621 tgaaatgaac atagccagtt cctacccaac cctgccctgc cttttttttt ttttttaaag
     1681 tgtggtcctg tgctttcaaa gtaagtacag ttctctatga ccaggcactt aaacataatt
     1741 taaaagtcct tgtagcttca gaaccggata agttccagtt gcatatctct atatctcctg
     1801 gtttccctct tggttttccc ctggttcctc ccctttccct cttggtggcc tgagttgttt
     1861 atgttggcag aaggttgaca ttggatgctc gattgcttca gcctcaccgg ggcattgaat
     1921 catcatttgt tgaatggcaa caggtttgct ggggcagagg cactgcctaa gtgggagcat
     1981 gcacgtgaaa atagaaaggt aatttaacaa cacacagaca cccagagact tggtctctgc
     2041 atgtgtgtag aagtagtggt gtggggaggg atgggactca ctcagccctg tgaatgtgtt
     2101 ctatggctga gcaacatccc tagccttcag aatagaattt ttgatgttgg aaatcctgta
     2161 tttaaaggaa accttaatta tcaatggatt tatgaaggaa agttaaaaac aaatatgcac
     2221 agtacaaaat agatctgtgg cttttttttt ttaaagtaag aatagcttgt tagtatatca
     2281 atgttcatgg ctaatgttat ctcattttgt tagtcgttat tttggaatta catgatgtgt
     2341 gatttagggc ttagttaatt ctctagaatt caaaaactat tattgatgta ttaaaagaaa
     2401 ttacggtacc acgttgatta gtctgaatgt ttgcaggcat tggaataaaa tttgacttat
     2461 tttttccctc ttcccctgtc aa
//
