LOCUS       NM_001007465            3506 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ring finger and FYVE like domain containing protein
            (Rffl), transcript variant 1, mRNA.
ACCESSION   NM_001007465
VERSION     NM_001007465.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Gan,X., Wang,C., Patel,M., Kreutz,B., Zhou,M., Kozasa,T. and Wu,D.
  TITLE     Different Raf protein kinases mediate different signaling pathways
            to stimulate E3 ligase RFFL gene expression in cell migration
            regulation
  JOURNAL   J Biol Chem 288 (47), 33978-33984 (2013)
   PUBMED   24114843
  REMARK    GeneRIF: EGF, which signals through CRAF, and an activated BRAF
            mutant also activate PKC and stimulate cell migration through
            up-regulating RFFL expression.
REFERENCE   3  (bases 1 to 3506)
  AUTHORS   Gan,X., Wang,J., Wang,C., Sommer,E., Kozasa,T., Srinivasula,S.,
            Alessi,D., Offermanns,S., Simon,M.I. and Wu,D.
  TITLE     PRR5L degradation promotes mTORC2-mediated PKC-delta
            phosphorylation and cell migration downstream of Galpha12
  JOURNAL   Nat Cell Biol 14 (7), 686-696 (2012)
   PUBMED   22609986
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3506)
  AUTHORS   Ahmed,A.U., Moulin,M., Coumailleau,F., Wong,W.W., Miasari,M.,
            Carter,H., Silke,J., Cohen-Tannoudji,M., Vince,J.E. and Vaux,D.L.
  TITLE     CARP2 deficiency does not alter induction of NF-kappaB by TNFalpha
  JOURNAL   Curr Biol 19 (1), R15-7;authorreplyR17-9 (2009)
   PUBMED   19138581
  REMARK    GeneRIF: CARP2 [Rififylin] deficiency does not alter induction of
            NF-kappaB by TNFalpha.
REFERENCE   5  (bases 1 to 3506)
  AUTHORS   Liao,W., Xiao,Q., Tchikov,V., Fujita,K., Yang,W., Wincovitch,S.,
            Garfield,S., Conze,D., El-Deiry,W.S., Schutze,S. and
            Srinivasula,S.M.
  TITLE     CARP-2 is an endosome-associated ubiquitin ligase for RIP and
            regulates TNF-induced NF-kappaB activation
  JOURNAL   Curr Biol 18 (9), 641-649 (2008)
   PUBMED   18450452
REFERENCE   6  (bases 1 to 3506)
  AUTHORS   Coumailleau,F., Das,V., Alcover,A., Raposo,G.,
            Vandormael-Pournin,S., Le Bras,S., Baldacci,P., Dautry-Varsat,A.,
            Babinet,C. and Cohen-Tannoudji,M.
  TITLE     Over-expression of Rififylin, a new RING finger and FYVE-like
            domain-containing protein, inhibits recycling from the endocytic
            recycling compartment
  JOURNAL   Mol Biol Cell 15 (10), 4444-4456 (2004)
   PUBMED   15229288
  REMARK    GeneRIF: Data show that the the amino-terminal domain of rififylin
            is critical for its recruitment to recycling endocytic membranes
            and for the inhibition of recycling.
REFERENCE   7  (bases 1 to 3506)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   8  (bases 1 to 3506)
  AUTHORS   Le Bras,S., Cohen-Tannoudji,M., Guyot,V., Vandormael-Pournin,S.,
            Coumailleau,F., Babinet,C. and Baldacci,P.
  TITLE     Transcript map of the Ovum mutant (Om) locus: isolation by exon
            trapping of new candidate genes for the DDK syndrome
  JOURNAL   Gene 296 (1-2), 75-86 (2002)
   PUBMED   12383505
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL646089.6, CJ097615.1, AK150213.1, AK169393.1 and AI850798.1.
            
            On Sep 3, 2009 this sequence version replaced NM_001007465.2.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169988.1, SRR13422601.157521.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                AL646089.6         15512-15523         c
            13-68               CJ097615.1         2-57
            69-1862             AK150213.1         2-1795
            1863-3136           AK169393.1         1723-2996
            3137-3487           AK169393.1         2998-3348
            3488-3506           AI850798.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 50.25 cM"
     gene            1..3506
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /note="ring finger and FYVE like domain containing
                     protein"
                     /db_xref="GeneID:67338"
                     /db_xref="MGI:MGI:1914588"
     exon            1..203
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     exon            204..391
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1303
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase rififylin; fring; RING finger and
                     FYVE-like domain-containing protein 1; RING-type E3
                     ubiquitin transferase rififylin"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase rififylin isoform 1"
                     /protein_id="NP_001007466.1"
                     /db_xref="CCDS:CCDS25148.1"
                     /db_xref="GeneID:67338"
                     /db_xref="MGI:MGI:1914588"
                     /translation="MWASCCNWFCLDGQPEEAPPPQGARTQAYSNPGYSSFPSPTGSE
                     PSCKACGVHFASTTRKQTCLDCKKNFCMTCSSQEGNGPRLCLLCLRFRATAFQREELM
                     KMKVKDLRDYLSLHDISTEMCREKEELVFLVLGQQPVISEADRTRVPHLPQAFPEQQA
                     FLTQPQTSTVPPTSPGLPSSPAQVTSVPLAQDQETQQAVGHVSQDHEEPIFPESTARV
                     PTEDETQSVDSEDSFVPGRRASLSDLTHLEDIEGLTVRQLKEILARNFVNYKGCCEKW
                     ELMERVTRLYKDQKGLQHLVSGNEDQNGGAVPSGLEENLCKICMDSPIDCVLLECGHM
                     VTCTKCGKRMNECPICRQYVIRAVHVFRS"
     exon            392..802
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     exon            803..886
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1097
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1121
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..3492
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1704
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /note="major polyA site"
     regulatory      3474..3479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /note="hexamer: AATAAA"
     polyA_site      3492
                     /gene="Rffl"
                     /gene_synonym="1700051E09Rik; 4930516L10Rik; Carp2"
                     /note="major polyA site"
ORIGIN      
        1 ggggcccacg ccgagccgcg ggggactggg ttagtggggg tggacttcct ggggccgccc
       61 gtgaggatgt ggttcgggca cccgggcgcc cctcaggcgt ggagttgcgg cggccgcaga
      121 ccccgcagcc ccgctgcctg agtggccgcg gtcgggatct ggcctctgcc gtcccaggtg
      181 tggcccggca gcgtccgcgg cagattttat catgtgggca tcctgctgca actggttctg
      241 cttggatgga cagcctgagg aggcaccacc accacaggga gccaggacac aagcctattc
      301 caaccctggg tacagctcct tcccttcccc cacaggctcg gaacctagct gcaaggcctg
      361 cggggtccat ttcgcaagca caacccggaa gcagacctgc ttggactgta agaaaaactt
      421 ctgcatgact tgttcgagcc aagaggggaa tgggccacgc ctctgccttc tctgcctacg
      481 gttccgagcc acggccttcc agcgggagga gctcatgaag atgaaggtga aggacctgag
      541 ggactatctc agcctccacg acatctctac ggaaatgtgc cgggagaaag aggagctggt
      601 gttcctggtg cttggccagc agcctgtaat ttctgaggcg gacaggactc gtgttcccca
      661 cttgccccag gccttccctg agcagcaggc cttcctgacc cagcctcaaa ccagcacagt
      721 gcctcctacc tcacctggcc tcccttcttc acctgcacaa gtcacctctg tccccttagc
      781 ccaggatcag gaaactcagc aggccgttgg ccatgtgtct caggaccacg aggagcccat
      841 cttcccggag agcacagcca gagtacctac tgaggatgag acccagtccg ttgactcaga
      901 ggacagcttc gtcccaggcc ggagggcctc gctgtctgac ctgacccacc tggaggacat
      961 tgaaggcctg accgtacggc agctgaagga gatcctggct cgtaactttg tcaactataa
     1021 gggctgctgc gagaagtggg agctgatgga gagggtgact cggctgtaca aggatcagaa
     1081 aggactccag cacctggtgt ctggtaatga agaccaaaac gggggagcag tgccttctgg
     1141 cctggaggag aacctgtgta agatttgcat ggactcaccc attgactgtg ttctgctgga
     1201 gtgtggccac atggtgacct gtaccaagtg tggcaaacgc atgaacgaat gtcctatctg
     1261 ccggcagtat gtgatcagag cagtgcacgt cttccggtcc tgatggctag cattggtttc
     1321 ttcagtgcct tacaggacat agcccacgat gtctgggctc agggttggtc caatacacaa
     1381 gggcagtcta ctgtgagcaa ctggcagtgt tcttaagact aggacaagca gagttgcctg
     1441 gcatgcttgt tttaggtggc agagcccaaa accagtggga actggttcac atcacatggg
     1501 tgaatgcctg catctgtgat cattggatgg tgatggtcat caatgaagag gtctttccag
     1561 aacgtggacc agtctctcct gtcctaagat ccatttcata catctgtaca ccactcctcc
     1621 tgtcaatcat aagttctgct agaaatggtc tttctcccac cccaccccca gcacatttgg
     1681 atttaaattt ctggtctctc tggctttctg tgtgttaatg tatcttttaa gtcttctcat
     1741 ttgcctaaaa cccatttctt tctatggacc aaaaaaatat gttaattggg ccaatagtat
     1801 tcctcaggtc agagtttgga tcagtaaact gacctctagt gagaaattaa gtcttaagtg
     1861 atatagaaaa aaattctagg ttgagtcact tctgtttagt attggggttg gaaagaacca
     1921 gtctctgacc taactgctgg gaagtccttc agagatggtg ctgtttctct ttgggtttga
     1981 ctttgtatca tccttaactt tggattatga ggaaagagaa atcaaggcag cggctttgtc
     2041 tttgtcttct ggtcctcatt ttccagcatg ctaaaatact ttaaaatttt ttctcttcct
     2101 ttggtcttaa atttgcttac tgaaagcata ggaacttcag caaggagctg ttctctagct
     2161 tggctgtaca tggagttctt ggaaagcaga cacacaggac agtgcatgag gcactggatg
     2221 tgttagacta tgactacggc tgctgaaaag tcttaactgc actctgttct cagctgctcg
     2281 ggcaggtcaa gacaagcatt ctttaagtca tgattgcagc cctcccaggg gctttacacc
     2341 actgacacag ctgagaacag ctcaacaaag cacaaaatat actatcagaa gagctgtgtg
     2401 tttctctgtg gggtgtacgc tcaacttggc taacactacc cttcactgtg tagctatgtg
     2461 agactggaat aaccaaccac tcactcatga ctgacttgga aaagtggagg gacgcctctt
     2521 aggggactat tagaaatgct taggcttctc cactgaattc cttcagagga tcaagtagtt
     2581 ccatgaagct gttgtcagag atgccaggcc cacttacctc tgcattcgaa gtccagatga
     2641 ccagattttg ggtcaaagct tttgtggatg tcagggattc agatccctcc acaaagactc
     2701 ccttgccctc cctccctgaa cagtgaatgc tgtcgtttac aatgtttcca gattagcatg
     2761 cccactgatt ttgctgctcc tggactccac tgtgaaacag gtctgatctt acagctcagt
     2821 aagacctaag aataacatta aagccggctg gctctccctc ataactgctt tctcttctga
     2881 ggccttatct ccctacctcc tgtacaggaa cagaaaatac actgatggct gtggttagca
     2941 acccaagtaa caaccagaac ccatgccatt taatttggcg atcttacctt taaatcttta
     3001 cccttggtgt tctcatttgg gggggaaaaa aaccaaaaca tgtcaaacaa ctttattgtt
     3061 aaaccaaatg aaagttggaa cccattccag ttaacatctc aggtagaaaa ccttaatttt
     3121 tttttttttt ttttttggtt aaatacctga gccccctttt tgttcttcac ggatccttgg
     3181 tcttaatgtt gaaacaccta atctttccaa ttattctgtg gattttgctg attgcaccaa
     3241 gatttcagtt gagacttagt tttcaaagat gctgtttcat aggttggaaa tagccggttc
     3301 taaactaata agctgtaaag gaattaatag tctatgttgt acaaatttgt tgagatctct
     3361 cggatgtaaa tgtgtagctc caagtggctt acattaagat tttctcacct gggtgttaaa
     3421 agcataccca aatgtgtatt tttttgttac agagctctgc tttataattt tgtaataaag
     3481 tttaaataca gaaaaaaaaa aaaaaa
//
