LOCUS       NM_026159               1963 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus retinol saturase (all trans retinol 13,14 reductase)
            (Retsat), mRNA.
ACCESSION   NM_026159
VERSION     NM_026159.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Kiefer,M.F., Meng,Y., Yang,N., Vahrenbrink,M., Wulff,S., Li,C.,
            Wowro,S.J., Petricek,K.M., Sommerfeld,M., Flores,R.E.,
            Obermayer,B., Piepelow,K., Klaus,S., Hartl,K., Guillot,A.,
            Tacke,F., Sigal,M. and Schupp,M.
  TITLE     Intestinal retinol saturase is implicated in the development of
            obesity and epithelial homeostasis upon injury
  JOURNAL   Am J Physiol Endocrinol Metab 327 (2), E203-E216 (2024)
   PUBMED   38895981
  REMARK    GeneRIF: Intestinal retinol saturase is implicated in the
            development of obesity and epithelial homeostasis upon injury.
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1963)
  AUTHORS   Tarban,N., Halasz,H., Gogolak,P., Garabuczi,E., Moise,A.R.,
            Palczewski,K., Sarang,Z. and Szondy,Z.
  TITLE     Regenerating Skeletal Muscle Compensates for the Impaired
            Macrophage Functions Leading to Normal Muscle Repair in Retinol
            Saturase Null Mice
  JOURNAL   Cells 11 (8), 1333 (2022)
   PUBMED   35456012
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1963)
  AUTHORS   Jiang,X., He,Y., Shen,Q., Duan,L., Yuan,Y., Tang,L., Shi,Y.,
            Liu,B., Zhai,H., Shi,P., Yang,C. and Chen,Y.
  TITLE     RETSAT Mutation Selected for Hypoxia Adaptation Inhibits Tumor
            Growth
  JOURNAL   Front Cell Dev Biol 9, 744992 (2021)
   PUBMED   34805153
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1963)
  AUTHORS   Sarang,Z., Saghy,T., Budai,Z., Ujlaky-Nagy,L., Bedekovics,J.,
            Beke,L., Mehes,G., Nagy,G., Ruhl,R., Moise,A.R., Palczewski,K. and
            Szondy,Z.
  TITLE     Retinol Saturase Knock-Out Mice are Characterized by Impaired
            Clearance of Apoptotic Cells and Develop Mild Autoimmunity
  JOURNAL   Biomolecules 9 (11), 737 (2019)
   PUBMED   31766264
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1963)
  AUTHORS   Moise,A.R., Dominguez,M., Alvarez,S., Alvarez,R., Schupp,M.,
            Cristancho,A.G., Kiser,P.D., de Lera,A.R., Lazar,M.A. and
            Palczewski,K.
  TITLE     Stereospecificity of retinol saturase: absolute configuration,
            synthesis, and biological evaluation of dihydroretinoids
  JOURNAL   J Am Chem Soc 130 (4), 1154-1155 (2008)
   PUBMED   18179220
  REMARK    GeneRIF: Mouse RetSat catalyzes the stereospecific saturation of
            the C13-C14 double bond of all-trans-retinol to produce
            (13R)-all-trans-13,14-dihydroretinol.
REFERENCE   7  (bases 1 to 1963)
  AUTHORS   Sun,Y., Ng,L., Lam,W., Lo,C.K., Chan,P.T., Yuen,Y.L., Wong,P.F.,
            Tsang,D.S., Cheung,W.T. and Lee,S.S.
  TITLE     Identification and characterization of a novel mouse peroxisome
            proliferator-activated receptor alpha-regulated and
            starvation-induced gene, Ppsig
  JOURNAL   Int J Biochem Cell Biol 40 (9), 1775-1791 (2008)
   PUBMED   18289917
  REMARK    GeneRIF: Here, we report the identification and characterization of
            Ppsig (for PPARalpha-regulated and starvation-induced gene) with
            unknown biological function from mouse liver
REFERENCE   8  (bases 1 to 1963)
  AUTHORS   Nagaoka-Yasuda,R., Matsuo,N., Perkins,B., Limbaeck-Stokin,K. and
            Mayford,M.
  TITLE     An RNAi-based genetic screen for oxidative stress resistance
            reveals retinol saturase as a mediator of stress resistance
  JOURNAL   Free Radic Biol Med 43 (5), 781-788 (2007)
   PUBMED   17664141
  REMARK    GeneRIF: suggest a novel role for retinol saturase in regulating
            sensitivity to oxidative stress
REFERENCE   9  (bases 1 to 1963)
  AUTHORS   Moise,A.R., Kuksa,V., Imanishi,Y. and Palczewski,K.
  TITLE     Identification of all-trans-retinol:all-trans-13,14-dihydroretinol
            saturase
  JOURNAL   J Biol Chem 279 (48), 50230-50242 (2004)
   PUBMED   15358783
REFERENCE   10 (bases 1 to 1963)
  AUTHORS   Wang,Y., Hu,L., Yao,R., Wang,M., Crist,K.A., Grubbs,C.J.,
            Johanning,G.L., Lubet,R.A. and You,M.
  TITLE     Altered gene expression profile in chemically induced rat mammary
            adenocarcinomas and its modulation by an aromatase inhibitor
  JOURNAL   Oncogene 20 (53), 7710-7721 (2001)
   PUBMED   11753649
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125039.4.
            
            On Jun 26, 2019 this sequence version replaced NM_026159.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF620365.1, EF620363.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC125039.4         73971-74165
            196-378             AC125039.4         76342-76524
            379-620             AC125039.4         76845-77086
            621-822             AC125039.4         78024-78225
            823-1020            AC125039.4         79023-79220
            1021-1140           AC125039.4         80235-80354
            1141-1279           AC125039.4         81341-81479
            1280-1389           AC125039.4         81754-81863
            1390-1556           AC125039.4         81942-82108
            1557-1716           AC125039.4         82243-82402
            1717-1963           AC125039.4         82611-82857
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.27 cM"
     gene            1..1963
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /note="retinol saturase (all trans retinol 13,14
                     reductase)"
                     /db_xref="GeneID:67442"
                     /db_xref="MGI:MGI:1914692"
     exon            1..195
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1856
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /EC_number="1.3.99.23"
                     /note="all-trans-retinol 13,14-reductase;
                     all-trans-13,14-dihydroretinol saturase;
                     PPAR-alpha-regulated and starvation-induced gene protein"
                     /codon_start=1
                     /product="all-trans-retinol 13,14-reductase precursor"
                     /protein_id="NP_080435.3"
                     /db_xref="CCDS:CCDS20244.1"
                     /db_xref="GeneID:67442"
                     /db_xref="MGI:MGI:1914692"
                     /translation="MWITALLLAVLLLVILHRVYVGLYAASSPNPFAEDVKRPPEPLV
                     TDKEARKKVLKQAFSVSRVPEKLDAVVIGSGIGGLASAAVLAKAGKRVLVLEQHTKAG
                     GCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITEGQLDWAPMASPFDLMILEGP
                     NGRKEFPMYSGRKEYIQGLKKKFPKEEAVIDKYMELVKVVARGVSHAVLLKFLPLPLT
                     QLLSKFGLLTRFSPFCRASTQSLAEVLQQLGASRELQAVLSYIFPTYGVTPSHTAFSL
                     HALLVDHYIQGAYYPRGGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVS
                     VKKGQELVNIYCPVVISNAGMFNTYQHLLPETVRHLPDVKKQLAMVRPGLSMLSIFIC
                     LKGTKEDLKLQSTNYYVYFDTDMDKAMERYVSMPKEKAPEHIPLLFIAFPSSKDPTWE
                     ERFPDRSTMTALVPMAFEWFEEWQEEPKGKRGVDYETLKNAFVEASMSVIMKLFPQLE
                     GKVESVTGGSPLTNQYYLAAPRGATYGADHDLARLHPHAMASIRAQTPIPNLYLTGQD
                     IFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVKAQKKKM"
     exon            196..378
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            379..620
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            621..822
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1020
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1140
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1279
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1389
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1556
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1716
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1963
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1941..1946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /note="hexamer: AACAAA"
     polyA_site      1963
                     /gene="Retsat"
                     /gene_synonym="0610039N19Rik; MMT-7; Ppsig"
                     /note="major polyA site"
ORIGIN      
        1 gcggtcttcc gagccctggc agcaacatgt ggatcactgc tctgctgctg gccgtgctgc
       61 tgctggtgat cctccacagg gtctacgtgg gcctttacgc tgcaagttcc ccgaacccct
      121 tcgccgagga tgtcaagcga ccgcctgaac ccctggtgac cgacaaggag gctaggaaga
      181 aagttctcaa acaagctttc tcagtcagcc gagtaccaga gaagctggat gcagtggtga
      241 tcggcagcgg cattggggga ctggcctcag ctgcggttct agctaaagct ggcaagagag
      301 tccttgtgct ggaacaacat accaaggcgg gcggctgttg tcataccttt ggggaaaatg
      361 gccttgaatt tgacactgga attcattata ttggacgaat gcgggagggc aacattggcc
      421 gttttatctt ggaccagatc actgaagggc aactggactg ggcccccatg gcctcccctt
      481 ttgacttgat gatactagaa gggcccaatg gccgaaagga gttccccatg tacagtggga
      541 ggaaagaata catccagggc cttaagaaga agttccccaa ggaagaagct gtcattgaca
      601 agtacatgga gttggttaag gtggtggccc gtggagtctc tcatgcagtt ctactcaagt
      661 tcctcccatt gcccttgact cagctcctca gcaagtttgg gctactgact cgtttctctc
      721 cattctgccg agcgtctacg cagagcctag ctgaagtcct gcagcagctt ggggcttccc
      781 gtgagctcca ggctgttctc agctacatct tccccactta cggagtaact cccagccaca
      841 ccgccttttc cttgcatgct ctgctggttg accactacat acaaggggca tattaccctc
      901 gagggggttc cagtgagatc gccttccata ccatcccttt gattcagcgg gccgggggcg
      961 ctgtcctcac cagggccact gtacagagtg tgctgctgga ctcagctggg agagcgtgtg
     1021 gtgtcagtgt gaagaaggga caagagctgg tgaacatcta ctgcccagtt gtcatctcca
     1081 atgcgggaat gttcaatacc tatcagcact tgttgccaga gactgtccgc catctgccag
     1141 atgtgaagaa gcagctggcg atggtaaggc ctggtctgag catgctctca atcttcatct
     1201 gtctgaaagg caccaaggag gacctgaagc ttcagtccac caactactat gtttattttg
     1261 acacagacat ggacaaagcg atggagcgct atgtctctat gcccaaggaa aaggctccag
     1321 aacacattcc ccttctcttc attgccttcc catcaagcaa ggatccaacc tgggaggagc
     1381 gattcccaga ccgatccaca atgactgcgc tggtacccat ggcctttgaa tggttcgagg
     1441 agtggcagga ggagccaaag ggcaagcgtg gtgttgacta tgagaccctc aaaaatgcct
     1501 tcgtggaagc ctctatgtcg gtgatcatga aactgttccc acagctggag ggcaaggtgg
     1561 agagtgtgac tggagggtca ccactgacca accagtacta tctggctgca ccccgaggag
     1621 ctacctatgg agctgaccat gacttggctc ggctgcatcc tcatgcaatg gcttccataa
     1681 gagcccaaac ccccatcccc aacctctacc tgacaggcca agatatcttc acctgtgggc
     1741 tgatgggggc cctgcagggg gccttgctgt gcagcagtgc catcctgaaa cggaacttgt
     1801 actcagatct gcaggctctt ggctcaaagg tcaaggcaca aaagaagaag atgtagtccg
     1861 ttcagagaag agccagagga aaggcacctc cccaacttct cgtggtgtcc tccctcctac
     1921 agcaattcct tgcacatata aacaaaaacc attttgtttc tga
//
