LOCUS       NM_009049                762 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus regulated endocrine-specific protein 18 (Resp18),
            transcript variant 1, mRNA.
ACCESSION   NM_009049
VERSION     NM_009049.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 762)
  AUTHORS   Bohuslavova,R., Fabriciova,V., Smolik,O., Lebron-Mora,L.,
            Abaffy,P., Benesova,S., Zucha,D., Valihrach,L., Berkova,Z.,
            Saudek,F. and Pavlinkova,G.
  TITLE     NEUROD1 reinforces endocrine cell fate acquisition in pancreatic
            development
  JOURNAL   Nat Commun 14 (1), 5554 (2023)
   PUBMED   37689751
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 762)
  AUTHORS   Huisman,C., Kim,Y.A., Jeon,S., Shin,B., Choi,J., Lim,S.J.,
            Youn,S.M., Park,Y., K,C. M, Kim,S., Lee,S.K., Lee,S. and Lee,J.W.
  TITLE     The histone H3-lysine 4-methyltransferase Mll4 regulates the
            development of growth hormone-releasing hormone-producing neurons
            in the mouse hypothalamus
  JOURNAL   Nat Commun 12 (1), 256 (2021)
   PUBMED   33431871
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 762)
  AUTHORS   Huisman,C., Cho,H., Brock,O., Lim,S.J., Youn,S.M., Park,Y., Kim,S.,
            Lee,S.K., Delogu,A. and Lee,J.W.
  TITLE     Single cell transcriptome analysis of developing arcuate nucleus
            neurons uncovers their key developmental regulators
  JOURNAL   Nat Commun 10 (1), 3696 (2019)
   PUBMED   31420539
  REMARK    Erratum:[Nat Commun. 2020 Mar 10;11(1):1359. doi:
            10.1038/s41467-020-14964-z.. PMID: 32157096]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 762)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 762)
  AUTHORS   Huang,Y., Xu,J., Liang,M., Hong,X., Suo,H., Liu,J., Yu,M. and
            Huang,F.
  TITLE     RESP18 is involved in the cytotoxicity of dopaminergic neurotoxins
            in MN9D cells
  JOURNAL   Neurotox Res 24 (2), 164-175 (2013)
   PUBMED   23319378
REFERENCE   7  (bases 1 to 762)
  AUTHORS   Erlandsen,S.E., Qvigstad,G., Fossmark,R., Bakke,I., Chen,D. and
            Sandvik,A.K.
  TITLE     Regulated endocrine-specific protein 18 (RESP18) is localized to
            and regulated in A-like cells and G-cells in rat stomach
  JOURNAL   Regul Pept 177 (1-3), 53-59 (2012)
   PUBMED   22561140
REFERENCE   8  (bases 1 to 762)
  AUTHORS   Liang,M., Yang,J.L., Bian,M.J., Liu,J., Hong,X.Q., Wang,Y.C.,
            Huang,Y.F., Gu,S.P., Yu,M., Huang,F. and Fei,J.
  TITLE     Requirement of regulated endocrine-specific protein-18 for
            development and expression of regulated endocrine-specific
            protein-18 isoform c in mice
  JOURNAL   Mol Biol Rep 38 (4), 2557-2562 (2011)
   PUBMED   21104147
  REMARK    GeneRIF: Requirement of regulated endocrine-specific protein-18 for
            development and expression of regulated endocrine-specific
            protein-18 isoform c in mice.
REFERENCE   9  (bases 1 to 762)
  AUTHORS   Zhang,G., Hirai,H., Cai,T., Miura,J., Yu,P., Huang,H.,
            Schiller,M.R., Swaim,W.D., Leapman,R.D. and Notkins,A.L.
  TITLE     RESP18, a homolog of the luminal domain IA-2, is found in dense
            core vesicles in pancreatic islet cells and is induced by high
            glucose
  JOURNAL   J Endocrinol 195 (2), 313-321 (2007)
   PUBMED   17951542
  REMARK    GeneRIF: RESP18 is a luminal protein of DCVs and its expression is
            regulated by exposure to glucose.
REFERENCE   10 (bases 1 to 762)
  AUTHORS   Bloomquist,B.T., Darlington,D.N., Mueller,G.P., Mains,R.E. and
            Eipper,B.A.
  TITLE     Regulated endocrine-specific protein-18: a short-lived novel
            glucocorticoid-regulated endocrine protein
  JOURNAL   Endocrinology 135 (6), 2714-2722 (1994)
   PUBMED   7988462
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC166150.2, AK012477.1 and
            AI838995.1.
            
            On Aug 12, 2016 this sequence version replaced NM_009049.1.
            
            Summary: This gene encodes a secreted protein that is expressed
            mainly in the peripheral endocrine and neuroendocrine tissues and
            is regulated by physiological factors that include blood glucose
            and dopaminergic drugs. The encoded protein is found in the lumen
            of the endoplasmic reticulum and is degraded in the post-ER
            pre-Golgi compartment. Gene knockout experiments in mice
            demonstrate that this gene is essential for embryonic development
            with embryonic lethality occurring before embryonic day 9.5.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Aug 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU604530.1, L34214.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AC166150.2         87503-87584
            83-363              AK012477.1         1-281
            364-365             AC166150.2         91433-91434
            366-545             AK012477.1         282-461
            546-546             AC166150.2         91922-91922
            547-681             AK012477.1         463-597
            682-762             AI838995.1         1-81                c
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.65 cM"
     gene            1..762
                     /gene="Resp18"
                     /note="regulated endocrine-specific protein 18"
                     /db_xref="GeneID:19711"
                     /db_xref="MGI:MGI:1098222"
     exon            1..214
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..636
                     /gene="Resp18"
                     /codon_start=1
                     /product="regulated endocrine-specific protein 18
                     precursor"
                     /protein_id="NP_033075.1"
                     /db_xref="CCDS:CCDS15069.1"
                     /db_xref="GeneID:19711"
                     /db_xref="MGI:MGI:1098222"
                     /translation="MQSSLKPAGSGHLQLLVCFLLLYSRPGSCSDINAHDGQGQVGSE
                     QLWTFQGLIASVFQYLQLIFHQIVPEGMFWADDIAYELMTKKVEHLSRLHPQYPCRKD
                     MKAVSPTANAGVRSKQEEKLQLLSPQKSPTVKVNRDRCFTTKVIPKATKQEATHPTKG
                     FFGPFPTVGLNLVAD"
     exon            215..319
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     exon            320..452
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     exon            453..495
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     exon            496..585
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     exon            586..750
                     /gene="Resp18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      726..731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Resp18"
                     /note="hexamer: ATTAAA"
     polyA_site      748
                     /gene="Resp18"
                     /note="major polyA site"
ORIGIN      
        1 tctagcgcta gagggtgaaa agtgacatcc tggcacaggc cccctggcta ttgttgctac
       61 ttccactacg acctagctcg aggaaccgtg agtttggatc cagccaagat gcagagttcg
      121 ttaaagcccg cgggctccgg gcatcttcag ctactggtct gcttccttct gctttacagt
      181 cgtccaggca gctgcagcga cataaacgcc cacgatggtc agggccaagt gggatcggaa
      241 cagctctgga cattccaagg acttattgct tcagtcttcc agtacttaca acttatattc
      301 caccagattg tacccgaagg catgttctgg gcagatgaca tagcctatga gttaatgacc
      361 aaaaaggtgg agcacctcag cagactccac ccccagtatc catgcaggaa ggatatgaag
      421 gcagtttccc ccacggcaaa cgctggggtg aggagcaagc aagaggagaa acttcaactc
      481 ttatctcccc aaaagagtcc aacagtcaag gtgaacaggg accgatgctt taccaccaaa
      541 gtaatcccaa aggccaccaa acaagaggcc acccatccta ccaagggttt ctttgggcca
      601 ttccccactg tgggcctcaa ccttgttgct gactgagatc tcgttgcata agatcccttt
      661 ctccgggaac ctcctcaatg ccctccaatc caaggggcaa ttcttggatg acccaaatat
      721 gaataattaa agttgatttt cctgcaaaca aaaaaaaaaa aa
//
