LOCUS       NM_001079904            3103 bp    mRNA    linear   ROD 04-AUG-2024
DEFINITION  Mus musculus replication initiator 1 (Repin1), transcript variant
            4, mRNA.
ACCESSION   NM_001079904
VERSION     NM_001079904.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Xia,Y., Ge,G., Xiao,H., Wu,M., Wang,T., Gu,C., Yang,H. and Geng,D.
  TITLE     REPIN1 regulates iron metabolism and osteoblast apoptosis in
            osteoporosis
  JOURNAL   Cell Death Dis 14 (9), 631 (2023)
   PUBMED   37749079
  REMARK    GeneRIF: REPIN1 regulates iron metabolism and osteoblast apoptosis
            in osteoporosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3103)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3103)
  AUTHORS   Abshagen,K., Degenhardt,B., Liebig,M., Wendt,A., Genz,B.,
            Schaeper,U., Stumvoll,M., Hofmann,U., Frank,M., Vollmar,B. and
            Kloting,N.
  TITLE     Liver-specific Repin1 deficiency impairs transient hepatic
            steatosis in liver regeneration
  JOURNAL   Sci Rep 8 (1), 16858 (2018)
   PUBMED   30442920
  REMARK    GeneRIF: Repin1 might be of functional significance for early
            accumulation of lipids in hepatocytes after partial hepatectomy,
            facilitating efficient progression of liver regeneration.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3103)
  AUTHORS   Kunath,A., Hesselbarth,N., Gericke,M., Kern,M., Dommel,S.,
            Kovacs,P., Stumvoll,M., Bluher,M. and Kloting,N.
  TITLE     Repin1 deficiency improves insulin sensitivity and glucose
            metabolism in db/db mice by reducing adipose tissue mass and
            inflammation
  JOURNAL   Biochem Biophys Res Commun 478 (1), 398-402 (2016)
   PUBMED   27402271
  REMARK    GeneRIF: Our study provides evidence that loss of Repin1 in db/db
            mice improves insulin sensitivity and reduces chronic hyperglycemia
            most likely by reducing fat mass and AT inflammation.
REFERENCE   6  (bases 1 to 3103)
  AUTHORS   Kern,M., Kosacka,J., Hesselbarth,N., Bruckner,J., Heiker,J.T.,
            Flehmig,G., Kloting,I., Kovacs,P., Matz-Soja,M., Gebhardt,R.,
            Krohn,K., Sales,S., Abshagen,K., Shevchenko,A., Stumvoll,M.,
            Bluher,M. and Kloting,N.
  TITLE     Liver-restricted Repin1 deficiency improves whole-body insulin
            sensitivity, alters lipid metabolism, and causes secondary changes
            in adipose tissue in mice
  JOURNAL   Diabetes 63 (10), 3295-3309 (2014)
   PUBMED   24760138
  REMARK    GeneRIF: Our findings indicate that Repin1 plays a role in insulin
            sensitivity and lipid metabolism by regulating key genes of glucose
            and lipid metabolism.
REFERENCE   7  (bases 1 to 3103)
  AUTHORS   Ruschke,K., Illes,M., Kern,M., Kloting,I., Fasshauer,M.,
            Schon,M.R., Kosacka,J., Fitzl,G., Kovacs,P., Stumvoll,M., Bluher,M.
            and Kloting,N.
  TITLE     Repin1 maybe involved in the regulation of cell size and glucose
            transport in adipocytes
  JOURNAL   Biochem Biophys Res Commun 400 (2), 246-251 (2010)
   PUBMED   20727851
  REMARK    GeneRIF: a potential role for Repin1 as regulator of adipocyte size
            was shown.Repin1 seems to be involved in the regulation of genes
            involved in adipogenesis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB870810.1, CA558513.1 and BC085128.1.
            
            Transcript Variant: This variant (4) differs in the 5' coding
            region, uses an alternate in-frame splice site, and uses an
            alternate start codon, compared to variant 1. The encoded isoform
            (d) has a distinct and longer N-terminus, compared to isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092301.1, CA558973.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                BB870810.1         2-98
            98-633              CA558513.1         1-536
            634-2674            BC085128.1         640-2680
            2675-3103           BC085128.1         2682-3110
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 23.57 cM"
     gene            1..3103
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /note="replication initiator 1"
                     /db_xref="GeneID:58887"
                     /db_xref="MGI:MGI:1889817"
     exon            1..170
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..1934
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /note="isoform d is encoded by transcript variant 4; zinc
                     finger protein 464 (RIP60); DNA-binding protein REPIN1"
                     /codon_start=1
                     /product="DNA-binding protein REPIN1 isoform d"
                     /protein_id="NP_001073373.2"
                     /db_xref="GeneID:58887"
                     /db_xref="MGI:MGI:1889817"
                     /translation="MDGRCVPGGHAGEFSLSSGGYRSVGRSSRSLPRNIPRRSWKKPH
                     PQLFSLQEEEPMLEQRCRGPTAMGPAQPWLFSGPSQESSQPDRGLRYQGKSAQPRGQT
                     PGKVHRCAHCRKRFPGWVALWLHARRCQARLPLPCHECNQRFRHAPFLALHLQVHASA
                     VPDLGFICHLCGHSFRGWVALVLHLRAHSASKRPITCPECDRRFWRQKQLRAHLRRCQ
                     PPVPEARPFICGNCGRSFAQWDQLVVHKRVHVAEALEEAAAKALGPRPRGRPAAPRPG
                     GDAVDRPFQCACCGKRFRHKPNLIAHRRVHTGERPHQCPECGKRFTNKPYLTSHRRIH
                     TGEKPYPCTECGRRFRHKPNLLSHSKIHKRLEVSAQAAPHPESHQIAAEPMAQPALGV
                     PLGSPRTPAEAPALLHSCSDCGRSFRLERFLRLHQRQHTGERPFACTECGKNFGKKTH
                     LVAHSRVHSGERPFACEECGRRFSQGSHLAAHRRDHAPERPFVCPDCGKAFRHKPYLA
                     AHRRIHTGEKPYVCPDCGKAFSQKSNLVSHRRIHTGERPYACPDCDRSFSQKSNLITH
                     RKSHIRDGAFCCAICGQTFDDEDRLLMHQKKHDA"
     exon            171..285
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /inference="alignment:Splign:2.1.0"
     exon            286..3072
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3049..3054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="Repin1"
                     /gene_synonym="Ap4; E430037F08Rik; Zfp-464; Zfp464"
                     /note="major polyA site"
ORIGIN      
        1 gtagtcacgt ggagctggga acccagcgcg tggggggagg ttggggacgg gcctggcagt
       61 tgtgaactcg aacctgccgc tgtcgccgct gcggggcggg gaaccggccg cagaggccgg
      121 cctggtgctc tatggatggg cgctgcgtcc ctggagggca cgcgggagag ttttctctgt
      181 catccggggg ctaccgaagt gtgggccgaa gcagccgaag tctccccagg aacatcccca
      241 gaaggagctg gaaaaagcct cacccccagc tcttcagtct tcaggaggaa gaaccgatgc
      301 tggaacagcg ctgcaggggc cccacggcca tgggcccagc tcagccctgg ctcttttctg
      361 ggccctccca ggagtcctcc cagcccgaca gagggttgag gtaccagggc aaatcagctc
      421 agccaagagg ccaaacccca ggcaaggtcc atcgttgtgc ccactgtcgg aagcgcttcc
      481 cgggctgggt ggccctgtgg cttcatgctc ggcggtgcca ggcccggctg cctctgccct
      541 gccatgaatg caaccagcgc tttcgccacg cccccttctt agcgctgcat cttcaggttc
      601 atgcttctgc agtccccgac ctgggtttca tctgccacct atgtgggcat agcttccgag
      661 gatgggtagc cctggttctg catctgcggg ctcactcagc ttcaaagcgg cccatcactt
      721 gccctgaatg cgacagacgc ttctggcgac aaaaacagct tcgagctcat ctgcgaaggt
      781 gccagccccc tgtccctgag gcccggccct tcatatgcgg caactgtggc cggagctttg
      841 cccaatggga ccaactggtt gttcacaagc gggtgcacgt ggctgaggcc ttggaggagg
      901 cagcagccaa agccctgggt cctcgcccac gaggacgtcc cgcagctccc aggcctggtg
      961 gagacgctgt ggaccgcccc ttccagtgtg cctgctgcgg caagcgtttc cgccacaagc
     1021 ccaatctgat cgcccaccgc cgcgtgcaca ctggtgagcg accacaccag tgcccagagt
     1081 gcgggaagcg tttcaccaac aagccctacc tgacctcgca ccggcgcata cataccggcg
     1141 agaagcccta cccatgcacc gagtgtggcc gccgcttccg ccacaaaccc aacctgttgt
     1201 cgcacagcaa aatccacaag cgcttggagg tctcagcgca ggctgcccca caccccgaga
     1261 gtcaccagat tgcagcagag cctatggcac aacctgcact tggggtgccc ctggggtccc
     1321 cgcggacccc agctgaggca cctgcgctcc tgcatagctg ctccgactgc ggccgcagct
     1381 tccggctgga gcgcttcctg cggctacacc agaggcagca cacaggggag aggcccttcg
     1441 cctgcacaga gtgtggcaag aacttcggca agaagacgca cctggtggcg cactcacgcg
     1501 tgcactccgg cgaacgtccc ttcgcctgcg aggagtgtgg tcgccgtttc tcacagggca
     1561 gccacctggc agcccaccgg cgagaccatg caccagagag gcccttcgtg tgcccggact
     1621 gcggcaaggc tttccgccac aagccctacc tggctgcgca ccgacgcatc cacacaggcg
     1681 agaaacccta tgtgtgtccc gactgtggca aagctttcag tcagaagtcc aacctggtgt
     1741 cccaccggcg catccacaca ggcgagcggc cctacgcctg ccccgactgt gatcgtagct
     1801 tcagtcagaa gtccaatctt atcacacacc ggaagagcca catccgggat ggcgccttct
     1861 gttgtgccat ctgtggccag acctttgatg acgaggaccg actcttgatg caccagaaga
     1921 agcatgatgc ctgagagggg ccgtaggcct aggaagacag gcctggatat ccttggcaac
     1981 agggtgtcag tggtccgggt ggggtaccta cactgatgct gctagaggaa caggtaaaga
     2041 tcccagaggg atagaggaga cagtgatctg ggctctgttt aggaatggac tttcccctag
     2101 gtatccaggg aacccatttt agaattcggg ggacctggct ttgagctagt tggcccccag
     2161 ccccagctgg tttttctaag ggtcaactca gactgccctg taccctggaa aaatagcaat
     2221 agcagcccca tctacccttt gagtcctcaa ctaaacacca gtgggaagag aaccgttcca
     2281 tcctctggtg ttaacgcctt aatgtcccca gtatttacta tgagttgctt caggtcattt
     2341 tttgattttt tgttttttct tttggaagta ggtgtggtat agtcgttagt aaatgaatcc
     2401 ctgggcaggg aatatgggct ggccggctgc accttggtga acctgctggt tagggcagca
     2461 gcagaggtga ggctgttcct ctctctctct gtctctctct ggtctctgtc tctctctctt
     2521 tttttaccag aacccttctg caatgcctcc tgcataggtc ccaagatgtg tagcgatcca
     2581 tccctggtta gcaattccat caatttcttg catattatcc tcccaggaca ttgatttact
     2641 cctttaccag cctggctttt tttttttttt ttttaatctg cctgctctcc cctaagtata
     2701 ggccttcctg gtggttttgt ttaaccccat gaccctcccc aactcttctc agagctttgg
     2761 gggttatgtg aaaggctgac tagttgaggg tttagctgca gggaaggtcc tgggtagatt
     2821 tcctttttaa ggttcccagg gtgtggatgc cagcctccac ccatgcacct gggtactggg
     2881 gcactgttac ctccagaagg cactacttct atcgtagggc gagtctaatt gttagacatt
     2941 tcttacttat agtgattgga ttctgctttc ctatgacttc tgcctactgg gtcttgtttt
     3001 gttccttgga tcgaaacaga accgtttacc cacctttaca aactagagaa taaagatttg
     3061 gttttagaac taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//
