LOCUS       NM_011992               2042 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus reticulocalbin 2 (Rcn2), transcript variant 1, mRNA.
ACCESSION   NM_011992
VERSION     NM_011992.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Liu,Y., Chen,P., Hu,B., Xiao,Y., Su,T., Luo,X., Tu,M. and Cai,G.
  TITLE     Excessive mechanical loading promotes osteoarthritis development by
            upregulating Rcn2
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (6), 167251 (2024)
   PUBMED   38795835
  REMARK    GeneRIF: Excessive mechanical loading promotes osteoarthritis
            development by upregulating Rcn2.
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2042)
  AUTHORS   Yao,H., Zhang,S., Xie,H., Fan,Y., Miao,M., Zhu,R., Yuan,L., Gu,M.,
            You,Y. and You,B.
  TITLE     RCN2 promotes Nasopharyngeal carcinoma progression by curbing
            Calcium flow and Mitochondrial apoptosis
  JOURNAL   Cell Oncol (Dordr) 46 (4), 1031-1048 (2023)
   PUBMED   36952101
  REMARK    GeneRIF: RCN2 promotes Nasopharyngeal carcinoma progression by
            curbing Calcium flow and Mitochondrial apoptosis.
REFERENCE   4  (bases 1 to 2042)
  AUTHORS   Peng,H., Hu,B., Xie,L.Q., Su,T., Li,C.J., Liu,Y., Yang,M., Xiao,Y.,
            Feng,X., Zhou,R., Guo,Q., Zhou,H.Y., Huang,Y., Jiang,T.J. and
            Luo,X.H.
  TITLE     A mechanosensitive lipolytic factor in the bone marrow promotes
            osteogenesis and lymphopoiesis
  JOURNAL   Cell Metab 34 (8), 1168-1182 (2022)
   PUBMED   35705079
REFERENCE   5  (bases 1 to 2042)
  AUTHORS   Li,J., Taylor,A.M., Manichaikul,A., Angle,J.F. and Shi,W.
  TITLE     Reticulocalbin 2 as a Potential Biomarker and Therapeutic Target
            for Atherosclerosis
  JOURNAL   Cells 11 (7), 1107 (2022)
   PUBMED   35406670
  REMARK    GeneRIF: Reticulocalbin 2 as a Potential Biomarker and Therapeutic
            Target for Atherosclerosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2042)
  AUTHORS   Wacker,B.K., Perfater,J.L. and Gidday,J.M.
  TITLE     Hypoxic preconditioning induces stroke tolerance in mice via a
            cascading HIF, sphingosine kinase, and CCL2 signaling pathway
  JOURNAL   J Neurochem 123 (6), 954-962 (2012)
   PUBMED   23043544
REFERENCE   7  (bases 1 to 2042)
  AUTHORS   Manichaikul,A., Wang,Q., Shi,Y.L., Zhang,Z., Leitinger,N. and
            Shi,W.
  TITLE     Characterization of Ath29, a major mouse atherosclerosis
            susceptibility locus, and identification of Rcn2 as a novel
            regulator of cytokine expression
  JOURNAL   Am J Physiol Heart Circ Physiol 301 (3), H1056-H1061 (2011)
   PUBMED   21666121
  REMARK    GeneRIF: Ath29 is confirmed to be a major atherosclerosis
            susceptibility locus affecting both early and advanced lesion
            formation in mice, and Rcn2 is identified as a novel regulator of
            cytokine expression.
REFERENCE   8  (bases 1 to 2042)
  AUTHORS   Li,L., Sun,L., Gao,F., Jiang,J., Yang,Y., Li,C., Gu,J., Wei,Z.,
            Yang,A., Lu,R., Ma,Y., Tang,F., Kwon,S.W., Zhao,Y., Li,J. and
            Jin,Y.
  TITLE     Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway
            and controls extraembryonic endoderm differentiation
  JOURNAL   Proc Natl Acad Sci U S A 107 (4), 1402-1407 (2010)
   PUBMED   20080709
REFERENCE   9  (bases 1 to 2042)
  AUTHORS   Porta,S., Marti,E., de la Luna,S. and Arbones,M.L.
  TITLE     Differential expression of members of the RCAN family of
            calcineurin regulators suggests selective functions for these
            proteins in the brain
  JOURNAL   Eur J Neurosci 26 (5), 1213-1226 (2007)
   PUBMED   17767500
  REMARK    GeneRIF: RCAN2 was highly expressed in scattered neurons, in both
            the nucleus and the cytoplasm. RCAN2 is the only member of the RCAN
            family that was detected in glial cells.
REFERENCE   10 (bases 1 to 2042)
  AUTHORS   Dodds,D., Schlimgen,A.K., Lu,S.Y. and Perin,M.S.
  TITLE     Novel reticular calcium binding protein is purified on taipoxin
            columns
  JOURNAL   J Neurochem 64 (5), 2339-2344 (1995)
   PUBMED   7722520
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB638743.1, AK077486.1, AC158999.2 and BB316373.1.
            
            On Sep 8, 2006 this sequence version replaced NM_011992.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            CCDS Note: This CCDS representation uses a downstream start codon
            that is well-conserved in mammalian organisms. It also contains an
            upstream in-frame start codon that would result in a 35 aa
            N-terminal extension. However, this upstream start codon is only
            present in mouse and rat, and its use would eliminate an N-terminal
            signal peptide that is only predicted in the shorter N-terminus.
            N-terminal sequencing of the rat protein in PMID:7722520 indicates
            signal peptide cleavage. It is likely that the upstream start codon
            is skipped due to leaky scanning by ribosomes, thereby permitting
            translation initiation at the currently represented start codon.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.236088.1, AK076153.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal (PMID:7722520)
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BB638743.1         2-26
            26-1847             AK077486.1         2-1823
            1848-1922           AC158999.2         133838-133912       c
            1923-2042           BB316373.1         190-309
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 30.13 cM"
     gene            1..2042
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /note="reticulocalbin 2"
                     /db_xref="GeneID:26611"
                     /db_xref="MGI:MGI:1349765"
     exon            1..360
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            361..466
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            467..663
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            664..777
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            778..874
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1017
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..2042
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1826..1831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /note="hexamer: AATAAA"
     polyA_site      1847
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /note="major polyA site"
     regulatory      2013..2018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
                     /note="hexamer: ATTAAA"
     polyA_site      2014
                     /gene="Rcn2"
                     /gene_synonym="Tcbp49"
ORIGIN      
        1 aaggcagggc ctggcgggcc tcagcaacgc agtagtcgac agacttcagg ctggggtgtg
       61 gagccccggc caccgccgcc actgacgacc cccgcccccc acatgcagcg ccgcccctct
      121 gtggctgccc gtgaagcaag gctgccaagc cccggctccg cctgcctccc tcctcgcggc
      181 tttctgcgtc ctcgccgagc gggcgcgatg cggctgggcc caaggcccgc ggcgctagga
      241 ctgctgctgc cgctgctgct gtacgccgcg gtggctggcg ccagcaaggc ggaggaactg
      301 cactacccgc agggcgagca ccgggcggac tacgaccgcg aagcgctgct gggtgtccag
      361 gaagacgtcg atgagtatgt taaacttggc cacgaagagc agcaaagacg attgcagtcg
      421 atcataaaga aaattgactc ggactctgat ggctttctta ctgaaaatga actcagtcag
      481 tggattcaga tgtcttttaa gcattacgct atgcaagaag ccaagcagca gtttgtggag
      541 tatgataaga acagcgacgg cgctgtgacg tgggatgagt acaacatcca gatgtacgac
      601 cgggtgattg actttgatga gaacactgct ctggatgaca cagaagaggg gtcgttcagg
      661 cagcttcatc taaaggataa gaagcgattt gaaaaagcta accaggattc aggtcctggt
      721 ctgagtcttg aagagttcat tgcgtttgag caccctgaag aagttgacta tatgacggag
      781 ttcgtcatcc aagaggcttt ggaagaacat gacaaaaatg gcgatgggtt tgttagtttg
      841 gaagaatttc ttggcgatta caggcgggat ccaactgcaa atgaagaccc agaatggata
      901 cttgttgaaa aggacagatt tgtgaatgat tatgacaaag ataatgatgg ccggcttgat
      961 ccccaagagc tgttgtcatg ggtagtgccc aataaccagg gcattgcaca agaggaagct
     1021 cttcatctga ttgatgagat ggatttgaat agtgacaaaa agctctctga agaagagatt
     1081 ctggaaaacc aagacttgtt ccttaccagc gaagccacgg attatggccg acagctccat
     1141 gacgactact tctatcatga cgagctttaa cccccgagtg cgccccagca ggatgcggcc
     1201 ttctgtagtt tgctaccagc ttgactttta tgatgagata tagatgttgt cctttacact
     1261 cttaagtctt accacttcag atgatctaaa tgtagattat aacttttggc cttttcaaag
     1321 aaaacaaaac aagaacttgt catttatttc aagacatatt gaaggagtaa actattaatg
     1381 ttgtgccata tgaccacaca gtctttccta agtactccat ctatgtagtg ctgtgttgtg
     1441 gaatgttgag ctctgttgcc atacttgaag acatggagga tttagacatg tctaaacaat
     1501 agtatttaag tagcacgtga tgaagctgtc agttttcgtt cttgtactag tagacttact
     1561 tttgaaactg acaaaggaca gtgtttagat ttagtgtttt atcttaaagc ctttaaagga
     1621 accttcacaa ggacttaaac ctcacataga tgttgagaag ttctacttaa ttttaaaatg
     1681 gtttctaaaa agggttttgt cgtatgaaat agaactttat atttttgcat atgtatagat
     1741 agtaattata tttaatgtat aactatagca ttatggtgtg tggaaattga cattgtccag
     1801 aactcttcac ttttgattga ttaaaaataa actgtcctat ctttttatat gtttgggttt
     1861 ttgttttttt tgttttattt ttccagataa atcttaaatt cagcatgggt tttgttatat
     1921 taaacattat tcattccttt attagatagt gaatgttctc aagattgtta attattcttg
     1981 gtacatagtc ttatttacct ttttttaaaa aaattaaaag aattatgtta taattgaacg
     2041 tg
//
