LOCUS       NM_001100591            8981 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus ring finger and CCCH-type zinc finger domains 2
            (Rc3h2), transcript variant 1, mRNA.
ACCESSION   NM_001100591 XM_130233 XM_925271
VERSION     NM_001100591.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Hirata,Y., Nakata,Y., Komatsu,H., Kudoh,Y., Takahashi,M.,
            Taguchi,S., Noguchi,T. and Matsuzawa,A.
  TITLE     Roquin-2 promotes oxidative stress-induced cell death by
            ubiquitination-dependent degradation of TAK1
  JOURNAL   Free Radic Biol Med 221, 31-39 (2024)
   PUBMED   38729452
  REMARK    GeneRIF: Roquin-2 promotes oxidative stress-induced cell death by
            ubiquitination-dependent degradation of TAK1.
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 8981)
  AUTHORS   Schmidt,H., Raj,T., O'Neill,T.J., Muschaweckh,A., Giesert,F.,
            Negraschus,A., Hoefig,K.P., Behrens,G., Esser,L., Baumann,C.,
            Feederle,R., Plaza-Sirvent,C., Geerlof,A., Gewies,A., Isay,S.E.,
            Ruland,J., Schmitz,I., Wurst,W., Korn,T., Krappmann,D. and
            Heissmeyer,V.
  TITLE     Unrestrained cleavage of Roquin-1 by MALT1 induces spontaneous T
            cell activation and the development of autoimmunity
  JOURNAL   Proc Natl Acad Sci U S A 120 (48), e2309205120 (2023)
   PUBMED   37988467
REFERENCE   4  (bases 1 to 8981)
  AUTHORS   Behrens,G., Edelmann,S.L., Raj,T., Kronbeck,N., Monecke,T.,
            Davydova,E., Wong,E.H., Kifinger,L., Giesert,F., Kirmaier,M.E.,
            Hohn,C., de Jonge,L.S., Pisfil,M.G., Fu,M., Theurich,S., Feske,S.,
            Kawakami,N., Wurst,W., Niessing,D. and Heissmeyer,V.
  TITLE     Disrupting Roquin-1 interaction with Regnase-1 induces autoimmunity
            and enhances antitumor responses
  JOURNAL   Nat Immunol 22 (12), 1563-1576 (2021)
   PUBMED   34811541
REFERENCE   5  (bases 1 to 8981)
  AUTHORS   Hoefig,K.P., Reim,A., Gallus,C., Wong,E.H., Behrens,G., Conrad,C.,
            Xu,M., Kifinger,L., Ito-Kureha,T., Defourny,K.A.Y., Geerlof,A.,
            Mautner,J., Hauck,S.M., Baumjohann,D., Feederle,R., Mann,M.,
            Wierer,M., Glasmacher,E. and Heissmeyer,V.
  TITLE     Defining the RBPome of primary T helper cells to elucidate
            higher-order Roquin-mediated mRNA regulation
  JOURNAL   Nat Commun 12 (1), 5208 (2021)
   PUBMED   34471108
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8981)
  AUTHORS   Schlundt,A., Heinz,G.A., Janowski,R., Geerlof,A., Stehle,R.,
            Heissmeyer,V., Niessing,D. and Sattler,M.
  TITLE     Structural basis for RNA recognition in roquin-mediated
            post-transcriptional gene regulation
  JOURNAL   Nat Struct Mol Biol 21 (8), 671-678 (2014)
   PUBMED   25026077
REFERENCE   7  (bases 1 to 8981)
  AUTHORS   Leppek,K., Schott,J., Reitter,S., Poetz,F., Hammond,M.C. and
            Stoecklin,G.
  TITLE     Roquin promotes constitutive mRNA decay via a conserved class of
            stem-loop recognition motifs
  JOURNAL   Cell 153 (4), 869-881 (2013)
   PUBMED   23663784
  REMARK    GeneRIF: Study demonstrates that the constitutive decay element
            folds into an RNA stem-loop motif that is specifically recognized
            by Roquin and Roquin2. Binding of Roquin initiates degradation of
            TNF-alpha mRNA and limits TNF-alpha production in macrophages.
REFERENCE   8  (bases 1 to 8981)
  AUTHORS   Vogel,K.U., Edelmann,S.L., Jeltsch,K.M., Bertossi,A., Heger,K.,
            Heinz,G.A., Zoller,J., Warth,S.C., Hoefig,K.P., Lohs,C., Neff,F.,
            Kremmer,E., Schick,J., Repsilber,D., Geerlof,A., Blum,H., Wurst,W.,
            Heikenwalder,M., Schmidt-Supprian,M. and Heissmeyer,V.
  TITLE     Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40
            costimulator mRNAs and control follicular helper T cell
            differentiation
  JOURNAL   Immunity 38 (4), 655-668 (2013)
   PUBMED   23583643
  REMARK    GeneRIF: Roquin-1 and Roquin-2 proteins redundantly repressed the
            mRNA of inducible costimulator (Icos) and identified the Ox40
            costimulatory receptor as another shared mRNA target.
REFERENCE   9  (bases 1 to 8981)
  AUTHORS   Pratama,A., Ramiscal,R.R., Silva,D.G., Das,S.K., Athanasopoulos,V.,
            Fitch,J., Botelho,N.K., Chang,P.P., Hu,X., Hogan,J.J., Mana,P.,
            Bernal,D., Korner,H., Yu,D., Goodnow,C.C., Cook,M.C. and
            Vinuesa,C.G.
  TITLE     Roquin-2 shares functions with its paralog Roquin-1 in the
            repression of mRNAs controlling T follicular helper cells and
            systemic inflammation
  JOURNAL   Immunity 38 (4), 669-680 (2013)
   PUBMED   23583642
  REMARK    GeneRIF: RING-deficient Roquin-1 failed to localize to stress
            granules and allowed Roquin-2 to compensate in the repression of
            ICOS and Tfh cells.
REFERENCE   10 (bases 1 to 8981)
  AUTHORS   Siess,D.C., Vedder,C.T., Merkens,L.S., Tanaka,T., Freed,A.C.,
            McCoy,S.L., Heinrich,M.C., Deffebach,M.E., Bennett,R.M. and
            Hefeneider,S.H.
  TITLE     A human gene coding for a membrane-associated nucleic acid-binding
            protein
  JOURNAL   J Biol Chem 275 (43), 33655-33662 (2000)
   PUBMED   10938276
  REMARK    GeneRIF: Characterization of the human RC3H2 ortholog and
            observation of conserved sequences in mouse.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK053071.1, BC117952.1, BC117951.1, AL929572.10 and BM898966.1.
            
            On or before Aug 11, 2007 this sequence version replaced
            XM_925271.3, XM_130233.8.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC117952.1, SRR10223969.1680.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AK053071.1         2-8
            8-3221              BC117952.1         1-3214
            3222-3940           BC117951.1         3215-3933
            3941-8494           AL929572.10        145479-150032       c
            8495-8981           BM898966.1         1-487               c
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 24.19 cM"
     gene            1..8981
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /note="ring finger and CCCH-type zinc finger domains 2"
                     /db_xref="GeneID:319817"
                     /db_xref="MGI:MGI:2442789"
     exon            1..137
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            138..173
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            174..470
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     CDS             240..3803
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1;
                     membrane associated DNA binding protein;
                     membrane-associated nucleic acid binding protein; ring
                     finger and CCCH-type zinc finger domain-containing protein
                     2; roquin-2; RING-type E3 ubiquitin transferase Roquin-2"
                     /codon_start=1
                     /product="roquin-2 isoform a"
                     /protein_id="NP_001094061.1"
                     /db_xref="CCDS:CCDS38116.1"
                     /db_xref="GeneID:319817"
                     /db_xref="MGI:MGI:2442789"
                     /translation="MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNK
                     LHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCV
                     EDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGE
                     RTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKV
                     LVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEAL
                     RREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQEL
                     TIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFI
                     QNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKM
                     SATVRTFPLLNKVGVNSTVTTTAGNVISVIGSTETTGKIVASTNGISNTESSVSQLIP
                     RGTDSAVRTLETVKKVGKVGTNAQNAGPSAESVSENKIGSPPKTPVSNAAATSAGPSN
                     FGTELNSLPPKSSPFLTRVPVYPQHSESIQYFQDPRTQIPFEVPQYPQTGYYPPPPTV
                     PAGVTPCVPRFVRSSNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQQYGPV
                     PPVPSGMYAPVYDSRRIWRPAMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDG
                     YYSVACQPPNDPRTTVPLPREPCGHLKTSCEEQLRRKPDQWTQYHTQKTPVSSTLPVA
                     TQSPTPPSPLFSVDFRSDFSESVSGAKFEEDHLSHYSPWSCGTIGSCINAIDSEPKDV
                     IANSNAVLMDLDSGDVKRRVHLFEAQRRTKEEDPIIPFSDGPIISKWGAISRSSRTGY
                     HTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNDATSSAHYIERDRFIVTD
                     LSGHRKHSSTGDLLSIELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLSKE
                     IELRNGENDYTEDTVDTKPDRDIELELSALDTDEPDGQSEQIEEILDIQLGISSQNDQ
                     LLNGTAVENGHPAQQHQKDPGKPKRQSLGEDHVILEEQKPILPVTSCFSQPRPMSISS
                     ASCLPITTSVSVGNLILKTHVMSEDKNDFLKPIANGKMVNS"
     exon            471..588
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            589..822
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            823..998
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1199
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1332
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1451
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1564
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1870
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..2085
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..2519
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2687
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2834
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..3048
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..3133
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3215
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3350
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3458
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3607
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     exon            3608..8967
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8942..8947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /note="hexamer: AATAAA"
     polyA_site      8967
                     /gene="Rc3h2"
                     /gene_synonym="2900024N03Rik; 9430019J22Rik;
                     D930043C02Rik; Mnab; Rnf164"
                     /note="major polyA site"
ORIGIN      
        1 gtagctcccg atggagtttg aggccctgaa accgccgccg agctctgccc cggcgagacg
       61 aggcgcctcc gtcgccgagc cgcgccgtcg cggggccccc gggagcggcc cttagcgacc
      121 ccgcccgggc ccctcagtac cggggattga atttaaagct tcacaaacat tagcttaaaa
      181 aacactacac atcaaaataa tgatctgcta gactgctaac ccgagcatca gcttccacaa
      241 tgcctgtgca ggcagctcaa tggacagagt ttctgtcctg tccaatctgc tataatgaat
      301 ttgatgagaa tgtgcacaaa cccatcagtt taggttgttc acacacagtt tgcaagacct
      361 gcttgaataa acttcaccga aaagcgtgtc cttttgacca gactgccata aacacagaca
      421 ttgatgtgct ccctgtcaac tttgcacttc ttcagttagt tggagcccag gtaccagatc
      481 atcagtcaat aaagttaagt aatctaggtg agaataaaca ctatgaagtg gcaaaaaaat
      541 gcgtagagga cttggcactc tacttaaaac cactaagtgg aggtaaaggt gtagctagtt
      601 tgaaccagag tgccctgagc cgtccaatgc aaaggaaact agttacactt gtaaactgtc
      661 aactggtgga agaagaaggt cgtgtcagag ctatgcgagc ggcccgttca ctaggagaaa
      721 gaactgtgac agaactgata ctgcagcacc agaaccctca gcagctgtct gccaacctgt
      781 gggctgctgt cagggctcga ggatgccagt ttctagggcc agctatgcaa gaagaagcct
      841 tgaagttggt attgctggca ttagaagatg gttctgctct ctctaggaaa gttctagtac
      901 tttttgttgt gcaaagacta gaaccaagat ttcctcaggc atcaaaaaca agtattggcc
      961 atgttgtgca gctattgtac cgagcttctt gttttaaggt tactaaaagg gatgaagact
     1021 cttccctgat gcagctaaag gaggaatttc ggagttacga ggcactacgc agagaacatg
     1081 atgcccagat tgttcatatt gccatggaag caggactccg tatttcacct gagcagtggt
     1141 cttcactgct gtatggggac ttggctcata aatcacacat gcagtctatc attgataagc
     1201 tacagtctcc agaatcattt gcaaagagtg tccaggaatt gacaattgtt ttgcagcgaa
     1261 ctggtgaccc tgctaattta aataggctga gacctcattt agagcttctt gcaaacatag
     1321 accctaatcc agatgctgtt tcaccaactt gggagcaact ggaaaatgca atggtagctg
     1381 ttaaaacagt tgttcatggt cttgtggact tcatacaaaa ttatagtaga aaaggccatg
     1441 agacacctca gccacagcca aacagcaagt ataaaactag tatgtgccga gatttacgac
     1501 aacaaggggg ttgtccacga ggaacaaatt gtacatttgc ccattctcag gaagagcttg
     1561 aaaagtaccg attaaggaac aaaaagatga gcgcgactgt aagaacattt cctcttctga
     1621 ataaagttgg tgtaaacagc actgtcacaa ccacagccgg aaatgtcatt tctgtcatag
     1681 gaagtactga aacaactggg aaaattgttg caagtacaaa tggaatttca aatacagaaa
     1741 gcagtgtttc tcagctaatc ccacgaggca ctgacagtgc agtaaggact ttggagactg
     1801 tgaagaaagt cgggaaggtt ggcactaatg ctcagaatgc tgggccctct gcagagtccg
     1861 tgtctgaaaa taaaattggt tctccaccca agactcctgt aagtaatgca gcagctacct
     1921 ccgctgggcc ctctaatttt ggaacagagc tgaattccct gcctcccaaa tccagcccat
     1981 ttctaactag agttccagta tatcctcagc attctgaaag cattcagtat tttcaagatc
     2041 caaggactca gatacctttt gaagtcccac aatacccaca aacaggatac tacccaccac
     2101 ctccaacggt accagctggt gtgactccct gtgttcctcg ctttgtgagg tccagtaatg
     2161 ttccagagtc ctccctccca cctgcttcca tgccatatgc tgatcattac agtacattct
     2221 cccctcgaga tcgaatgaat tcttctcctt accaacctcc tcctccgcag cagtatggac
     2281 cagttcctcc agtaccctct ggaatgtatg ctccggtgta tgacagcagg cgcatctggc
     2341 gcccagctat gtaccaacga gatgacatta ttagaagcaa ttctttacct ccaatggatg
     2401 tgatgcactc atctgtctat cagacatctt tacgggaaag atacaactca ttagatggat
     2461 attattcagt ggcttgtcag ccaccgaatg acccaaggac aactgtgcct ttaccaaggg
     2521 aaccttgtgg tcatttgaag accagttgtg aggaacagtt aagaagaaag ccagatcagt
     2581 ggacacagta tcatacccag aaaacacctg tctcttcaac tcttcctgtg gccacacagt
     2641 caccaacacc accttctcct ctattcagtg tagacttccg atcggatttc tctgagagtg
     2701 tgagtggcgc aaaatttgag gaagaccatc tttcccatta ttctccctgg tcttgtggta
     2761 ccataggctc ttgtataaat gccattgatt cggagcccaa agacgtaatt gctaattcaa
     2821 atgctgtatt gatggacctg gacagtgggg atgtaaagag aagagtgcat ttatttgaag
     2881 ctcagagaag gacaaaagaa gaagacccaa ttattccctt tagtgacgga cccattatct
     2941 caaaatgggg tgcaatttcc cgatcctccc gaacaggtta ccatacaaca gatcccgtcc
     3001 aggcaactgc ttcccaagga agtgcaacta agcccatcag cgtatcagat tatgtccctt
     3061 acgtgaatgc tgtcgattca aggtggagtt catatggcaa cgatgctaca tcatcagcac
     3121 actatattga acgggacaga ttcattgtta ctgatttatc tggtcataga aagcattcta
     3181 gtaccggaga ccttttgagc attgaacttc agcaggccaa gagtaactca ttacttcttc
     3241 agagggaagc taatgctctg gccatgcaac aaaagtggaa ttccctggat gaaggccgtc
     3301 accttacttt aaatcttcta agcaaggaaa ttgaactaag aaatggagag aatgactaca
     3361 cagaagacac tgtagataca aagcctgata gggatattga gttagagctt tcagcccttg
     3421 atactgatga acctgatggc cagagtgaac aaattgaaga aattttggac atacaacttg
     3481 gtatcagttc tcaaaatgac cagttgctca atggaacagc agtggaaaat ggtcatccag
     3541 cccagcagca ccagaaggac ccaggaaagc cgaagagaca gagtttaggt gaagaccatg
     3601 tgattctgga ggagcaaaaa ccaattctgc cggtaacatc ttgctttagc cagccacgtc
     3661 caatgtctat tagcagtgca agctgcctcc ccatcaccac atctgtcagt gttggcaacc
     3721 tcattctgaa aactcacgtt atgtctgaag ataaaaacga ctttttaaaa cctattgcaa
     3781 atgggaagat ggttaacagc tgaaaggagg ctcagctttc aaatctgtga ccacaccatg
     3841 gaagcattta cactagcttt ttatatatat aatatatatt atataatgta tatttttttt
     3901 aaaaaaaaag atattactgg gggcatccat ttcctgtgga ctctttgata cttcaagccc
     3961 tcttgcatta gcattatgaa aaaaaagaaa atttacttta ctagggtaat gttcactttc
     4021 cagaaattat tggaatttgg acaacattta acttaagctt caagcagctt tttatgagtt
     4081 tgtagcaatc cggtgcagta actgagccat ttcctatttt aaatggcttc tacagtaaat
     4141 taacaactca gttattaaac tagcaggatc ctacaatgat acatctagtg aaagtagagt
     4201 taattaactt atttctattt tatgtttcac tgagagaaat ttccatctca ttccagctgc
     4261 tttatttatc tttttcattg gactttgcat ggatttttct ttctttcttt ttttgttttg
     4321 ttttgttttg tttttccttt tcttttctgt cttttttttt tttttttgag agtggagtta
     4381 gatgttctta ccatagagtt ttacagggtt ataatagctt tctttactgc attatatgta
     4441 gaagtgtagt gcagtgcttt tatctgtagc atttaatttt ttaattttcc atttttcaag
     4501 aatagtttct gctttgttaa tatttataca caggctactt gttgaagtaa ttaattaaaa
     4561 atataaccaa gtgcctaagt ctttcagctg atgtactaga tattaaattc tcctaagtta
     4621 tgcagaatcg tttttacaat tttgataaat tatgcactaa tgtaatggca attttttaaa
     4681 atgcagattt aagcctgtac attattgaat ctgatgactt ggcaaaagaa tcaggtgctt
     4741 tcagctttct tgagaaaacc ctgtatgtag cgtttgcact gactaacaag atggtattgc
     4801 tgggtcttta atttaaagaa attaatttgg gtgttcattg cattatttag ttagatattg
     4861 tttattgtta cttcatgttg ggtttaattt tcttgttttc tgactgttag gttgataaga
     4921 ctttgaacca tgtagtagta gaacattggc taacattacc catacttaaa aacatgaata
     4981 atttctgccc tgccataatg tgactgaaat tttcacttgg catctgaaag tatgcagtat
     5041 gttcaaccag ctctgcccca gatttgtggg tagtagttta ttgaagctta aatttcagtc
     5101 aatatttaaa aacaaaagca gggcatgcct ttctggtagt aatctaatag tttatctttt
     5161 tgtttttctt ccatttcttt aagattagtt tgacttttat tattttttaa ttaacttctg
     5221 tgaagttgtt tactctgaaa attgtactgg aatattttaa gccaagctga tctttcacca
     5281 ggtgtactgt atgtaagggc ttctcctgct agcaagatgc ttaaacagga tcatgttatt
     5341 ggtcgtctga gctatttagt tattttacaa aaaccacagc attgtatcag agaagatttt
     5401 gataaaagtt ttgtgcacat ttccaggggt gggagggtta acatttccct gaaatttctt
     5461 gattggaatg agtaaatact ggtgtttgat acctgtctta atgaacatgc tcataaggtg
     5521 actgatacgt tgtaaataat acctgagaaa caaaggggta gttttgatat tctggtgtca
     5581 gtatggaaga tcttacacat ttatttaata ctcaagtgta tgaagatgtt tgtttataac
     5641 ataacagcac agtagccttg acttagtttt atttggttag tactgtacct ttttgccatg
     5701 gccagtgctc cccatcccta caaagacatt ttatttattt tactctgtaa cctgaaatga
     5761 agaggaacaa gagttttaaa ccatgttaca ttaacttatt tctgacatta taaattagcc
     5821 taggatatta caggaacatg attgttatat aatttatatc agaacctttc tataaatatg
     5881 agcttattac atttgaaatg tttccaatgt atttttcttt tccttctctt ctcttctctt
     5941 ctcttctctc ttctctcttc tctcttctct cttctctctt cttttctttt cttttctttt
     6001 cttttctttt cttttctttt cttttctttt cttttctttc tgtttgtaat tccaaaaagg
     6061 ttatgtaaac cagtctgtct atttcatgga tatttaatag tgagatcttc catgttgaag
     6121 gtaatgtatt tttttttaag attttaaaat tgctattttg ttacaaaata gagacctatt
     6181 aacagttgag agctccttat gtgccctcag ggaaagaacc ctctgtgcag cagagctaca
     6241 cgaacatctt cagcactttc tgagggtgat tatatactac ataaattgat cttgtgtatt
     6301 taaccttatc tttttaattt taaagttgaa attttgaaac gtggttgtgc tcttctggag
     6361 gggttgggat gcttaggtgt ttgcctactt gttcagtaaa ctgcatttca tcagtgtatg
     6421 tgtacacctg ccaaccttat tggtgtgtct caaacattta taggaaaata gtgcttgtct
     6481 tgcagcagat gatcccataa aacaataact tctaattctt aaatgtttac tctttctcac
     6541 taacaacaca ggaattgttt ctgtgttcac attattctgt attgcatttt ttatgtggtg
     6601 acagcttact gtatgtttta gtgggtataa tttgaaataa ggctattgga tctctgccac
     6661 ttaagaagaa agaacagttc atctgacctt tcttcaaaat gatacctgct gatgtcacag
     6721 aggaaaatag tacaaacaga cctagaatgt gttttttttt aattggggca atcatcactt
     6781 tgacccttta tcaagatgac atgatgaaca catttggaat cagaggctac aaaacaatag
     6841 aagagaaact ttgtaatgtc ggtatctaaa atgcttgtaa agcctatggg tctaatcagg
     6901 aatcagtagt atgaagtctg aaaaaaagca gggaaatggt gtaggtttcc ttatattttt
     6961 tacctgtcag ttttcggagt cccaattcct agtctgtgtg ctgcattcag atgctgtgaa
     7021 tatatcatta gttttgtgtg ggcttctttt attttatgaa ttctaagagt tctgtgtata
     7081 tttcttctag ttggtaccta cacaaattag atttctcttg cttttgccac tatgacttat
     7141 ttcagtgcca tgcaacgagt cagtgtatgt agggacaggg acattgtaca ttgtaatttt
     7201 caagaaaagg tatctcttaa ggcccgtggc actgattctg ctttcatagc tacttactct
     7261 actaagagaa ttcaatgaaa cttcatttaa aaactaaaat ttcccattgg tatggatcgt
     7321 agtcactttg acccagattt caagttgatg ctaagtttac aaagcacatc ctgattttat
     7381 atacagaaga ttacagaatg caatgatttg taggaccttt gagtgggatc atttaactat
     7441 ttgaaaaaat tgtttaaaaa ccatctaccc tatttttgca ttttagatgt gttaaagccc
     7501 atgttgtctt tttagccagg gatagaactt gtaacatcca gattcagtat gttagttgta
     7561 aatctgagta tttcttccct tgcatctttg agtcattttc taagcagact gtcaccaata
     7621 ttggtaatta atcattaaag agaaaagttg cattgcagct atatattagt gtcttagaac
     7681 tgttcttttg ttttagtgaa taaagagtac caatgggatc acttactgtt aactccttct
     7741 actgaagggc cccttctgca agcagaacac aaatgaacat gctcaaggag tatcccgttt
     7801 tctagagatt aagtttgcta gtaactcctt tacactagct atttctttgt atccctttgc
     7861 tggcaaggga gtacaaggag tcaaaccaca gatcagaaca ccccacattt gagtggagac
     7921 ttatttttac tctaagggga gttattcccc caagtctgaa actggcaatt attttttttt
     7981 ctcttacata aaaatttaaa aatatccccc aaaccagtgg aaaacagaca ctggctgcac
     8041 ttagtactgc caagagccaa ggtcatttgc acatattcca tcaacctgtc aaaaattagg
     8101 cctcacttta taacccaagg catggaagtg catgcattct cttagctggg caagcaatta
     8161 tactgtagtt gtgatacaac acatgtggct tttatttgta ctgcacatac ccactgtaca
     8221 gccacttggg agtatcgtgg ttagcttgca gcaactgccg tctgcattta tactgtttat
     8281 tgcatattct tttccctgga agtgaaagag aaatgttttt cttgttgcat tgattacatt
     8341 ttataaattt gcttagctgg aaagtttggg aaaagaggcc tgtttgtcaa ttgtacaacc
     8401 gattgtgaag cttagtgtga atatttttac gtctgtatta gacattttct ttgcaaatct
     8461 attgttcgat tgaaatgtaa atgaaattaa agatggtgta cacccatcat gtaaaacgca
     8521 ggcaccatct ctaagatgga tttaatgctc atttttaagg catatactca gcttctattt
     8581 aaaactataa aataattctg tacaatgaaa tggggaatat atatgggaat aaattctatt
     8641 ccatttattt caatttgaat ttccaaattg taatgtttcc ctttgtgcta taggaatagg
     8701 attaaatggg ggaaggctag gacttataag gcctgtatat ggggggaggg cagagatgga
     8761 acaatgaggg ttgtgatgat agtgaatagc aaagagtgaa ttctgtgtgt ttttgctgta
     8821 gcactgaagt gaagagatat tagctttggc tgttcacaaa atagagcatc atgattttca
     8881 gtgtttgaga gaaaattgat ggaaaaagtt tgcagtactt gacatgtatt tgcatgcaca
     8941 aaataaaatt atttgtccac cttaaaaaaa aaaaaaaaaa a
//
