LOCUS       NM_001024952           11011 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus RING CCCH (C3H) domains 1 (Rc3h1), mRNA.
ACCESSION   NM_001024952 XM_355250
VERSION     NM_001024952.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Schmidt,H., Raj,T., O'Neill,T.J., Muschaweckh,A., Giesert,F.,
            Negraschus,A., Hoefig,K.P., Behrens,G., Esser,L., Baumann,C.,
            Feederle,R., Plaza-Sirvent,C., Geerlof,A., Gewies,A., Isay,S.E.,
            Ruland,J., Schmitz,I., Wurst,W., Korn,T., Krappmann,D. and
            Heissmeyer,V.
  TITLE     Unrestrained cleavage of Roquin-1 by MALT1 induces spontaneous T
            cell activation and the development of autoimmunity
  JOURNAL   Proc Natl Acad Sci U S A 120 (48), e2309205120 (2023)
   PUBMED   37988467
  REMARK    GeneRIF: Unrestrained cleavage of Roquin-1 by MALT1 induces
            spontaneous T cell activation and the development of autoimmunity.
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Zhao,H., Liu,Y., Wang,L., Jin,G., Zhao,X., Xu,J., Zhang,G., Ma,Y.,
            Yin,N. and Peng,M.
  TITLE     Genome-wide fitness gene identification reveals Roquin as a potent
            suppressor of CD8 T cell expansion and anti-tumor immunity
  JOURNAL   Cell Rep 37 (10), 110083 (2021)
   PUBMED   34879274
  REMARK    GeneRIF: Genome-wide fitness gene identification reveals Roquin as
            a potent suppressor of CD8 T cell expansion and anti-tumor
            immunity.
REFERENCE   3  (bases 1 to 11011)
  AUTHORS   Behrens,G., Edelmann,S.L., Raj,T., Kronbeck,N., Monecke,T.,
            Davydova,E., Wong,E.H., Kifinger,L., Giesert,F., Kirmaier,M.E.,
            Hohn,C., de Jonge,L.S., Pisfil,M.G., Fu,M., Theurich,S., Feske,S.,
            Kawakami,N., Wurst,W., Niessing,D. and Heissmeyer,V.
  TITLE     Disrupting Roquin-1 interaction with Regnase-1 induces autoimmunity
            and enhances antitumor responses
  JOURNAL   Nat Immunol 22 (12), 1563-1576 (2021)
   PUBMED   34811541
  REMARK    GeneRIF: Disrupting Roquin-1 interaction with Regnase-1 induces
            autoimmunity and enhances antitumor responses.
REFERENCE   4  (bases 1 to 11011)
  AUTHORS   Hoefig,K.P., Reim,A., Gallus,C., Wong,E.H., Behrens,G., Conrad,C.,
            Xu,M., Kifinger,L., Ito-Kureha,T., Defourny,K.A.Y., Geerlof,A.,
            Mautner,J., Hauck,S.M., Baumjohann,D., Feederle,R., Mann,M.,
            Wierer,M., Glasmacher,E. and Heissmeyer,V.
  TITLE     Defining the RBPome of primary T helper cells to elucidate
            higher-order Roquin-mediated mRNA regulation
  JOURNAL   Nat Commun 12 (1), 5208 (2021)
   PUBMED   34471108
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11011)
  AUTHORS   Park,M.J., Baek,J.A., Choi,J.W., Jang,S.G., Kim,D.S., Park,S.H.,
            Cho,M.L. and Kwok,S.K.
  TITLE     Programmed Death-Ligand 1 Expression Potentiates the Immune
            Modulatory Function Of Myeloid-Derived Suppressor Cells in Systemic
            Lupus Erythematosus
  JOURNAL   Front Immunol 12, 606024 (2021)
   PUBMED   33986739
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11011)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   7  (bases 1 to 11011)
  AUTHORS   Yu,J.H., Yang,W.H., Gulick,T., Bloch,K.D. and Bloch,D.B.
  TITLE     Ge-1 is a central component of the mammalian cytoplasmic mRNA
            processing body
  JOURNAL   RNA 11 (12), 1795-1802 (2005)
   PUBMED   16314453
REFERENCE   8  (bases 1 to 11011)
  AUTHORS   Vinuesa,C.G., Cook,M.C., Angelucci,C., Athanasopoulos,V., Rui,L.,
            Hill,K.M., Yu,D., Domaschenz,H., Whittle,B., Lambe,T.,
            Roberts,I.S., Copley,R.R., Bell,J.I., Cornall,R.J. and Goodnow,C.C.
  TITLE     A RING-type ubiquitin ligase family member required to repress
            follicular helper T cells and autoimmunity
  JOURNAL   Nature 435 (7041), 452-458 (2005)
   PUBMED   15917799
  REMARK    GeneRIF: Negative regulator of follicular helper T cells.
            Represses ICOS expression on T cells.
REFERENCE   9  (bases 1 to 11011)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   10 (bases 1 to 11011)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ133431.1, AY948287.1 and AC119204.7.
            
            On Oct 4, 2007 this sequence version replaced NM_001024952.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK173335.1, AK139648.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                CJ133431.1         2-60
            60-3889             AY948287.1         1-3830
            3890-11011          AC119204.7         18695-25816         c
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 69.75 cM"
     gene            1..11011
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /note="RING CCCH (C3H) domains 1"
                     /db_xref="GeneID:381305"
                     /db_xref="MGI:MGI:2685397"
     exon            1..289
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            290..670
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     CDS             440..3832
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /EC_number="2.3.2.27"
                     /note="roquin; protein Sanroque; RING finger and C3H zinc
                     finger protein 1; probable E3 ubiquitin-protein ligase
                     Roquin; RING finger and CCCH-type zinc finger
                     domain-containing protein 1"
                     /codon_start=1
                     /product="roquin-1"
                     /protein_id="NP_001020123.1"
                     /db_xref="CCDS:CCDS15410.1"
                     /db_xref="GeneID:381305"
                     /db_xref="MGI:MGI:2685397"
                     /translation="MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNK
                     LHRKACPFDQTTINTDIELLPVNSALLQLVGAQIPEQQPITLCSGVEDTKHYEEAKKC
                     VEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARS
                     LGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALS
                     RKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTY
                     EALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSV
                     QELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLV
                     DYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMN
                     KRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLI
                     PRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLP
                     PMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFV
                     RPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTR
                     EEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRL
                     PPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAG
                     KYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRR
                     AVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSA
                     YGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLN
                     HQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVE
                     REIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSA
                     LTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP"
     exon            671..791
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            792..1031
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1207
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1408
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1541
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1660
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1773
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..2055
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2270
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2638
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            2639..2803
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..2956
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3170
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3261
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3394
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3565
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            3566..3681
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     exon            3682..11011
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10987..10992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /note="hexamer: ATTAAA"
     polyA_site      11009
                     /gene="Rc3h1"
                     /gene_synonym="5730557L09Rik; Gm551; mKIAA2025"
                     /note="major polyA site"
ORIGIN      
        1 ggagtctgag gaagaggaag gggacgagga caacgagcga cgggcggcgg aggacgcgga
       61 ggaggaaggg gaggccgacc aacaagatgg cccctgctgc aggagcagcc tcagcagcag
      121 caggtggggg gctggaggcg gcgctggcgg tggcggctac ggccgtggtc cggcggcctg
      181 ggacagagtc agagtcgatg gtggcagcgg cggcggcggc ggctgccgcg acggtggcga
      241 cggccgcagg gactcggagc tgagaggagc tgctgctcgg accagagcgg ttatatttca
      301 cactatgtgc tgactgtatc tacagaagat atttaaaaaa aagataaact tttttccaag
      361 attttgattc cagtggaatc tttgctttag attttttgtg tgtgtgtgtg cgtgttagtg
      421 aattgtaact ccagaagcaa tgcctgtaca agctccacaa tggacggatt tcctttcctg
      481 cccaatttgc actcagactt tcgacgaaac aattcgaaag cccatcagtt tgggctgtgg
      541 ccatactgtc tgcaaaatgt gcctgaataa actccaccgt aaggcttgcc catttgacca
      601 gaccactatc aatacggaca ttgagcttct tcctgtgaac tcagcattac tgcagctagt
      661 gggtgctcag atccctgagc aacagcccat tactttgtgt agtggggttg aagatacaaa
      721 gcattatgag gaagccaaga aatgtgtaga agaattagca ttgtacctga agccactcag
      781 cagtgcgaga ggtgtgggtc taaatagcac tactcagagt gttctgagtc gcccaatgca
      841 gaggaaactt gtgactctgg ttcattgcca gctagtggaa gaagaaggca ggatccgtgc
      901 tatgagggct gctcgatctt taggtgaacg cacagttaca gagctcattc tccagcacca
      961 gaatcctcag caactctctt ctaatctctg ggcagcagtc agagctaggg gctgccagtt
     1021 ccttggacca gcgatgcagg aggaagctct gaagctggtc ttgctggcct tagaagatgg
     1081 ttctgcattg tctcggaaag tgttggttct cttcgtggtg caaagactgg agccacgttt
     1141 tcctcaagcc tctaaaacca gcattgggca tgttgttcag ctcctctaca gagcctcctg
     1201 cttcaaggtc accaaacgtg atgaagactc ttctttgatg caactgaaag aagaatttag
     1261 gacttacgaa gctctgagga gagaacatga ctcccagata gtgcaaattg ctatggaagc
     1321 tggcttaagg attgcaccag accagtggtc ctctttgctt tatggggatc agtctcacaa
     1381 atctcatatg cagtctatta ttgacaagtt acagacccct gcctcatttg cacagagtgt
     1441 tcaggagcta acaattgctc tccagcggac tggagaccca gcgaatttga accgactaag
     1501 accccatttg gagttactcg caaacattga ccctagtcca gatgctcctc ctccaacatg
     1561 ggaacagcta gaaaatgggc tggttgctgt acgtacggta gtgcatggat tggttgatta
     1621 tatccagaac cacagcaaaa aaggagcaga tcaacaacag cctccacagc atagcaaata
     1681 caaaacatac atgtgtcgag atatgaagca aaggggagga tgccctcgtg gtgccagctg
     1741 tacgtttgca cattcacagg aagaactgga aaagtttcgt aaaatgaaca aacgtctggt
     1801 tcccagaaga cccttgagtg cctctttggg tcaacttaat gaggtgggcc tgccttcagc
     1861 acctatactt tctgatgaaa gtgcagtgga cttgtccaac agaaaacctc ctgcactccc
     1921 aaatggaatt gcatcgtcag ggagcacagt gacacaactg attccacgtg ggacagaccc
     1981 cagctttgat tcttctctga aaccagtaaa gctagaccac ctgagcagta gtgctccggg
     2041 gtcccctcct gacctgctgg aatctgcccc taagagtatt tctgccttac ctgtgaatcc
     2101 acatcctgta cctccaaggg gaccaacaga tctgcctccc atgcctgtca ccaaaccaat
     2161 tcagatggta cctcgaggtt ctcagctata tccagcacaa caagcagatg tttattatca
     2221 ggatcctcga ggaagtgccc cagcttttga gacagcacct taccagcagg gtatgtacta
     2281 tactccacca ccatgtgtgt cccgctttgt ccgacctcca ccatctgctc ctgaacctgg
     2341 tcctccatac ttggatcatt attcacccta cctccaagat cgtgtcataa actctcagta
     2401 tggcacacag ccacaacagt acccacctat gtacccagct cactacgatg gccgccgtgt
     2461 ataccctgct cagtcttaca caagagagga gatgttccgg gaaagcccta tacccattga
     2521 cattccatct gcagcagtgc cgtcctatgt acctgagtcc agagaacggt accaacaggt
     2581 agagggttac tacccggtgg ctcctcatcc agctcagatc agaccttcat accccaggga
     2641 tcctccttat agtcggcttc ctcctcccca accccatcct agtctggatg agctacatcg
     2701 aaggcggaag gaaataatgg cccagctaga ggaaagaaaa gtcatctctc cacctccttt
     2761 tgcaccgtca ccaacattgc ctcctgcatt ccatccagag gaatttttgg atgaagactt
     2821 gaaggtagcc ggaaaataca aagcaaatga ttatagccag tattctcctt ggtcatgtga
     2881 taccatcggc tcctacattg gaaccaaaga tgcaaaaccc aaagatgttg tggcagcggg
     2941 gagtgtggaa atgatgaatg tagaaagtaa aggaacgaga gagcagaggt tggaccttca
     3001 gagaagagca gtggagacga gcgatgatga cctcatcccg tttggagacc gaccaacagt
     3061 ctctcgattc ggtgccatct ctcgaacatc caaaacactg tatcagggtg ctggcccact
     3121 gcaagctata gcacctcagg gagcacccac aaaatctatt aacatttccg attacagtgc
     3181 atatggagct catggtggct ggggagattc tccatactca cctcatgcaa acatacctcc
     3241 tcagggacac ttcattgaga gggagaaaat gtccatggca gaagtggcca gtcatggaaa
     3301 accccttcta tctgctgaaa gagaacaatt acggctagaa ttgcagcagc tgaaccatca
     3361 gatcagccag cagacccagc tacgaggact agaggctgtt agtaaccggc tggtgttaca
     3421 gagggaggtg aacaccctgg caagccagcc acagccccct cagctgcctc caaaatggcc
     3481 tggaatgatc tcaagtgagc agctcagctt ggaactgcat caggtggaaa gagaaattgg
     3541 gaagaggacc cgggaattga gtatggagaa ccagtgttct gtagacatga aaagcaaact
     3601 gggtacaagc aagcaagcag aaaatggcca gcccgagccc caaaacaaga tccgcactga
     3661 ggaccttaca ttgacattca gtgatgtacc aaatggatca gccttgacac aagagaatct
     3721 cagcctccta tcaaacaaga ccagctcttt gaatctgtca gaagactctg agggaggagg
     3781 agataacaat gattcccaga gatcaggagt tgtttccaat tctgctccct agaatatggg
     3841 cacctgcttc taccttctgc tcctaatcag ttcatggggc atagggatag aaaaatggat
     3901 aacttctaaa ctttttctct gagtggtcag tgaagtccag gaaaagcacc ggaggcaatg
     3961 tgcacaaaca ccactactaa acaaacctgc acatagttca cattgatgca tttttttaat
     4021 tttaagaaaa aaaaattagc agtatctgca agcaattcag attaaatttg tttttgttct
     4081 gtgaatgaac tttattttaa taactttgga cgccttggat ccggggcttt aaaaaaaaag
     4141 aagatattat atatacactc tgtttgatta attgtatgat cttaatgaag taggtttttc
     4201 ttgtattttg gtattattac atacccagtg tataattcct tttccccagt cctttccaac
     4261 tctcacaacc ctgtaaacta aaatagacaa aaatgcaccg tttccaagaa ttctgtggta
     4321 atagattggg ttattagaaa ctgtgcagca aactaatttt attagagcta ttctttaacc
     4381 ccagccctgt ttctggatca ctagctctgt ggatagatac ttgtttttta caacattaaa
     4441 agtttatttg agtggatatg gttttagttt caacttttca gagatgtcaa catgcagttg
     4501 attgtttgag ccacattttg gtgtcaagca ttccttgcat tatctcctta aaatctgaaa
     4561 gcctatttag tgtttacaat gtttagttag atttaaatga agattatttt attttaagaa
     4621 tgaactctga attggatata atggctcatt cctgtaatcc ctggcctcaa gatgttgagg
     4681 caggaaaaaa cagaataaga agaaaagaaa tgaacttcag cttgttaata gtgaaaatag
     4741 gcatttcctg ttctcctatg tggagctcat gttggaaaat tcgagctgtg tttacaaatt
     4801 aaacttgtct tgaaacccta gaatagaaaa accaggacag tgaaccatac tctaaagctg
     4861 ggagccggct cagcagaaga gcaccggctg ctctcccggg ttcttggcag cacctgcagg
     4921 gtgtctccca gttccacttt cagggacatg acaccctttt ctggcccttg tggacaccag
     4981 acacaatgaa gaaaattttt taattaaaat aaaacttaac actcgatagg agcttcagta
     5041 aacaaggaca tattatttca atggaatttt tgttttcctt tcaaaagcgt taaagatttg
     5101 gcagatagaa actgactgag tcttcagggt agttcatgct ttggtcactt gtcagtaatc
     5161 agaattacta gaataactat aaataacttg aagtgagttt ttatagacct ggctttctga
     5221 cattaaaaat gtttatgaaa aagttgaaga gtgaggaaga atttttattt agttctgctg
     5281 cacatattct aatgctaacc actgtttgct taatttacta agcccctcta aaatgagttc
     5341 agcaaggcaa tgtttcgtag aatcactcca tccctctgat tgcctgacgt cagtttccct
     5401 ttctagatga tgctacaaaa caagaaagca ctggttatgt aataagttac tgcattgctt
     5461 tggttgggta aaagcaaacg tttatatttt tcttatttct atttcctgaa cccttaactc
     5521 tgctgaggtc tgcgtcacct cagtctaagc tctgtgtcca tcatcttgtt aattgaaaca
     5581 agtgggtggg ggtgggataa gagtgggagg ggtgggaagg gaaaaatagt cctgccattg
     5641 cctaccagta ggaaatccaa acagaacact catttgagta gcgattattt aatttgccca
     5701 gtcaaggcac cgtgtttata tactatttca cattgaattt gattatgccc tacagacctg
     5761 gctggtcaag gatttgatat acacatattg gcttgggatt cgagctttct tttttattta
     5821 aataaaaatt tatatatata ttatatatat acatatatac atggctatat ctgtatatat
     5881 atcgggtatg ttttaaagat ttcttcgcat gagcgcagct gttgcaataa gatgactgat
     5941 tgggaggtag aggcaccgaa tgacggtgag gcatttctca gttgatgcat acattttcct
     6001 tttaatttta aatacacatt tgtacatttg ctttttttgg actttttttt ttaagataaa
     6061 atgactctta catttatact ttactcatta tttattgaga tgaggagaga aatgttatgc
     6121 ctgacaggat ttttattttt ctgcatatct ataaaacgcc acattattgg cctcttgcct
     6181 tcagtagaca gatttgggtg ggtcttacct cctttggaca tttcaagata attcctaagt
     6241 cttagaagca gatgggtttt tgttgttgtt gtttttaaaa ttggaataac tagggggaaa
     6301 tctgcatatt ggttttcttg gagttaagaa taaaagtaaa ttttcattgt agtgtgtatg
     6361 agacccccaa aatgtactct gctgcggtgc actagtatcc ttttaggtat atttttattt
     6421 agggagaaaa ttagtctttt aaatggtagt atgagttccc caagcccctc tctatagcca
     6481 gtctgggttg ggtagaaagt gaaagactgt gcctacaatt ttgctattta gtcattttcc
     6541 cttaccaatt tttaggtggg gttttttggg gagcaggggg taggcagtat tgtttgtgcc
     6601 cactggcagg tttagggatt caataaaaac tctcataact ttgaggggtt tttctcccct
     6661 taacaaagtc aatgttatga gacaacaata aattgaagca taacttcatg actcttaact
     6721 gctgttttta ttaaaatatg aaaaccattg atttttattt gtgtctttag agacattaaa
     6781 aaaaggaagc ccatcaaata ggaagcagag cttctgcacc accagccctg cccttttaag
     6841 acagtcttag cagatagccc atactggttc tgactcgaga ccctgcgcca gccttgtgag
     6901 cgagcactgg aagaatgtca agcatgtgcc gccatgctca gcatactcag caatgggtat
     6961 ggcttttcac tgaaatggtt tcttctgaag gtgattactt tttactgtat ccaagagcaa
     7021 gaatcatctc agatgaaaca gacaattctg gaagggttat gcagcagcat attcatactt
     7081 gttcagaaac aacgccagta tgttagctgt cgtgaataaa aagtacttag gctcagtctg
     7141 ttttccgtga gtctctggaa catgatggag gaactgagaa tggggtatct agactcagtt
     7201 tcctgttgat agtaggagtc tgaatgtgac caaagagcca ttccttacta cttttctact
     7261 taaacagaac ttgtaccata ggtttctggt agtgatgttc aaatgacgtc aatgtttaag
     7321 acactattat tagtaattac aaatattcac ctactcagaa tgtgtaagag cttgtttggc
     7381 acgtacggca ttaatgtggg tcattccttc tctcatcttc cttcccttgt ggttttccca
     7441 tgtatttatt cttttgccta acagcagaag atggatacaa ttctcacgtg ctacttggat
     7501 acatgatccc atcagactcc catgagcttg agtgtgggag cttgaaattt agttcctaag
     7561 tttcccatga ttgtcttttt tcttttcttc ctgacccctt gccattctgc agtttcccct
     7621 tgacttaccc aatctgtaac agtgacagtt tgctgcttct aaacagagca ttcaaatgaa
     7681 ttcgcttagc caataacttc tgtgaagttg ccttttctaa tggcgttatt actcagtgat
     7741 gcacagatgt ggtatttgcc ctactggcag gcaagcaaag gtctggcaaa gaatgataac
     7801 ctgctatttc tttaaggagc gccaaagact tgtgctttac tctgttttgg ttttagggga
     7861 aatagtagcc attttgatta tgaaatctgg tagtatttgt aagttgattg tactgtgtaa
     7921 atgtctaaag ttaactgtta aggttagctg gccttacaaa atgtaaaagt aatgaaaatt
     7981 tatcagtttc tctaaaccag taaatatcac ccttgacaat atttgtttaa gcaactcact
     8041 ctccctttgg gggcttatct tgatgtacta ctctcttctg tgcgtctctg tactttcact
     8101 tgtgcctaca ggacaatgta ctttcactat ggtcaagtcc tgggtctggg aattcttctt
     8161 gaacttgacg cttgtgagaa acagttaacg gtagttactt agtacctgtt cgacttaagt
     8221 tcagggacat cttattttta ctaaatggga ttcatttgct attgttttta tgtacaaatg
     8281 atggtagaat gaaatttcaa tatatttttc atttgggctt ttaaagaatc aagtaaagag
     8341 ttcaatatgt aaaatctttg tgtaaaaacc atccgtaacg atttattaca tcctaatttg
     8401 ttctcaagag gaagtaatga ccttgcaaat taactgcagt attacactat tgccctataa
     8461 aaggttcaag tgtgttaaac tcttaattgt taatatatgc ttatagtgtt gcaaagggat
     8521 gtcatccctg aagatagagc tcagatacag cgtcactgag acaacgggca gtctacactg
     8581 acaccatttc atctgaaaca tatcgatgtc taccttagac agctatggct tttttttttt
     8641 tttttaacag ctcagctttt taaaatttcc tgctgaaatg actggctggg agtttttggt
     8701 aaattgtgct aacattcttt ttaacgttct caatcagctt tcctgagcca ctcacttcaa
     8761 agagctggtt ttgttttgtt ttgttcaaaa gatacaggaa ataacatttg agattttaag
     8821 attctatttc tgtaattgct agaggtgttt tcacaagtaa cagattttaa ttaattagta
     8881 attctagtca cactaataaa gaataggaaa ctgctacccc caggagcaaa tgacaccttc
     8941 taataagtgt tctcagtagg taggtttcaa tactacactg aataagtggg taaagagccc
     9001 ttggaatccc attgtgataa agggacttcc cttagcactg gaattgttct tagagcctct
     9061 ggatttcaga caaacactga atttccctga acttaacctt gagcagaata accttcattt
     9121 tcacaagaaa gtaaggcatt gtaacccaag gtggaaaact taaactttaa gacaatactg
     9181 tgtcagaaaa atgtgtattt agaagagtga aaccctgtgg agtctgttgt cctagtgtat
     9241 attatattat atagtagata aggtggcttt ttttttttta ttttttattg aagactcatt
     9301 ctggttcttc atttggtttt gtgttttgtc ttttaatgct acatatgtag acagagctca
     9361 caagtctttg gctcccatat taggttgtac actcagagtc atcttataac tggaaactgt
     9421 caggtggtcc cagctccagt gaacacagga aaagtcaagg aaaggatgca agactgggtg
     9481 aatagcgctc tggggttttg gttttgtttt ggtcctcctt ttaaaaattg tcatttttat
     9541 aatttattgg tgtagcctct ctctctgttc attggctgac atttcacagt aatatccaaa
     9601 ccaccccata aaattgtatt cttacaagcc tttaaagcta tgattcctgt ttagctaaga
     9661 cagtcctgag aggttttggg gtttgttttg ttttggtttg tttttgcctc actttcccct
     9721 tacccttttt tatcctttgt cttcccaact ttctggatat aaaagaaaaa aaaacttcac
     9781 tggtcttagg cctcagtaat gagtctgact ttccaggagt gatacaggaa gtgaactaag
     9841 gtaatgagca aacttctcac ccacctaact gctttccagt tgtgaggttc ctcttcgtgt
     9901 gtggtctttt cccttttacc cctgaaaccc ttccctgcct atggtcagtt ggtctcacgg
     9961 ctcacaccag tctcctgaga cattctgcac tcattatcac ctttttgggt ggatttttct
    10021 tagttttttt gttttgtttt taaaaaataa ctttaacatt gattcatatt tgcttgggat
    10081 agagcttatg taatttacca atcgtattga ttgtatgtga ttgtgccctg cagaggtata
    10141 cttaacatga gtaatcttaa gttagtaaag gagctatgag atttgagtca gaatagagat
    10201 ggctttatgg ggagtgtgaa gtcccttctc agaatcagtg ctacggaaga ccccctttga
    10261 aaggttttcc tccattcctg tgtttttcca ttgcctcggc tccctattgt tttctgacat
    10321 tttctcccca cctgctcaca aggtttttta aattgagtct taaaataaga gataactttt
    10381 aactgaatga attttgtgaa attttacatt ttccaaatgt tttcaattga ggacaaagga
    10441 tgggctggac ttggccagcc tgtgaccatc tccttcccat ctggcctggc caacagtgag
    10501 atttgcattt agaggcatta tcttctgaaa gttgtttttg tataggaaaa aaaagaacct
    10561 tgcttgaaat ggaacattcc ctttctttaa tctgtttaat atttccgttt ttaaacagct
    10621 ttcaaaacaa atgaggtggg gaaaaaagaa ctaggagtgt gtttagggct gccgacctct
    10681 ggatctgaac gagtacatgg accatgtgtt gcatattctt gagaagatta aggccttctc
    10741 tcttaatttt gtattctttg tgtgcatgtt gtttatatta aagatggcac attttgttaa
    10801 aatgctattt cctttattac cttggaaact gactcagcct cttgttgctc ctaattaggg
    10861 tttaaggctc ttgtaagttg cagataaaag gattcatttt aacaatagaa ggaggtggtt
    10921 caccttttgg attgtaaata tatgaaagtg tctaaaggtc tttatctgct ttctgtcagc
    10981 atttatatta aatgataaat taatgaggag g
//
