LOCUS       NM_019712               1655 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus ring-box 1 (Rbx1), mRNA.
ACCESSION   NM_019712
VERSION     NM_019712.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Bao,E., Zhou,Y., He,S., Tang,J., He,Y., Zhu,M., Cheng,C. and
            Wang,Y.
  TITLE     RING box protein-1(RBX1), a key component of SCF E3 ligase, induced
            multiple myeloma cell drug-resistance though suppressing p27
  JOURNAL   Cancer Biol Ther 24 (1), 2231670 (2023)
   PUBMED   37639640
  REMARK    GeneRIF: RING box protein-1(RBX1), a key component of SCF E3
            ligase, induced multiple myeloma cell drug-resistance though
            suppressing p27.
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wu,D., Li,H., Liu,M., Qin,J. and Sun,Y.
  TITLE     The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are
            essential for the maintenance of Regulatory T cell fitness
  JOURNAL   Nat Commun 13 (1), 3021 (2022)
   PUBMED   35641500
  REMARK    GeneRIF: The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are
            essential for the maintenance of Regulatory T cell fitness.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1655)
  AUTHORS   Nakayama,S., Yumimoto,K., Kawamura,A. and Nakayama,K.I.
  TITLE     Degradation of the endoplasmic reticulum-anchored transcription
            factor MyRF by the ubiquitin ligase SCFFbxw7 in a manner dependent
            on the kinase GSK-3
  JOURNAL   J Biol Chem 293 (15), 5705-5714 (2018)
   PUBMED   29472293
REFERENCE   4  (bases 1 to 1655)
  AUTHORS   Ye,B., Liu,B., Hao,L., Zhu,X., Yang,L., Wang,S., Xia,P., Du,Y.,
            Meng,S., Huang,G., Qin,X., Wang,Y., Yan,X., Li,C., Hao,J., Zhu,P.,
            He,L., Tian,Y. and Fan,Z.
  TITLE     Klf4 glutamylation is required for cell reprogramming and early
            embryonic development in mice
  JOURNAL   Nat Commun 9 (1), 1261 (2018)
   PUBMED   29593216
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1655)
  AUTHORS   Li,Z., Pei,X.H., Yan,J., Yan,F., Cappell,K.M., Whitehurst,A.W. and
            Xiong,Y.
  TITLE     CUL9 mediates the functions of the 3M complex and ubiquitylates
            survivin to maintain genome integrity
  JOURNAL   Mol Cell 54 (5), 805-819 (2014)
   PUBMED   24793696
REFERENCE   6  (bases 1 to 1655)
  AUTHORS   Kamura,T., Burian,D., Yan,Q., Schmidt,S.L., Lane,W.S., Querido,E.,
            Branton,P.E., Shilatifard,A., Conaway,R.C. and Conaway,J.W.
  TITLE     Muf1, a novel Elongin BC-interacting leucine-rich repeat protein
            that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin
            ligase
  JOURNAL   J Biol Chem 276 (32), 29748-29753 (2001)
   PUBMED   11384984
REFERENCE   7  (bases 1 to 1655)
  AUTHORS   Perin,J.-P., Seddiqi,N., Charbonnier,F., Goudou,D., Belkadi,L.,
            Rieger,F. and Alliel,P.M.
  TITLE     Genomic organization and expression of the ubiquitin-proteasome
            complex-associated protein Rbx1/ROC1/Hrt1
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (8), 1131-1137 (1999)
   PUBMED   10643962
REFERENCE   8  (bases 1 to 1655)
  AUTHORS   Skowyra,D., Koepp,D.M., Kamura,T., Conrad,M.N., Conaway,R.C.,
            Conaway,J.W., Elledge,S.J. and Harper,J.W.
  TITLE     Reconstitution of G1 cyclin ubiquitination with complexes
            containing SCFGrr1 and Rbx1
  JOURNAL   Science 284 (5414), 662-665 (1999)
   PUBMED   10213692
REFERENCE   9  (bases 1 to 1655)
  AUTHORS   Kamura,T., Koepp,D.M., Conrad,M.N., Skowyra,D., Moreland,R.J.,
            Iliopoulos,O., Lane,W.S., Kaelin,W.G. Jr., Elledge,S.J.,
            Conaway,R.C., Harper,J.W. and Conaway,J.W.
  TITLE     Rbx1, a component of the VHL tumor suppressor complex and SCF
            ubiquitin ligase
  JOURNAL   Science 284 (5414), 657-661 (1999)
   PUBMED   10213691
REFERENCE   10 (bases 1 to 1655)
  AUTHORS   Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ082632.1, BC056992.1, CB522825.1 and BB807275.1.
            
            On Aug 12, 2006 this sequence version replaced NM_019712.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005127.1, BC027396.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                CJ082632.1         21-77
            58-955              BC056992.1         1-898
            956-1004            BC056992.1         900-948
            1005-1620           CB522825.1         21-636
            1621-1650           BC056992.1         1563-1592
            1651-1655           BB807275.1         367-371
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 38.11 cM"
     gene            1..1655
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /note="ring-box 1"
                     /db_xref="GeneID:56438"
                     /db_xref="MGI:MGI:1891829"
     exon            1..135
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..384
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /EC_number="2.3.2.27"
                     /EC_number="2.3.2.32"
                     /note="ring-box protein 1; RING finger protein 75; E3
                     ubiquitin-protein transferase RBX1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RBX1"
                     /protein_id="NP_062686.1"
                     /db_xref="CCDS:CCDS49676.1"
                     /db_xref="GeneID:56438"
                     /db_xref="MGI:MGI:1891829"
                     /translation="MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAI
                     CRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWE
                     FQKYGH"
     exon            136..214
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /inference="alignment:Splign:2.1.0"
     exon            215..285
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /inference="alignment:Splign:2.1.0"
     exon            286..371
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..1655
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      528..533
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /note="hexamer: AATAAA"
     polyA_site      547
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /note="major polyA site"
     regulatory      991..996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /note="hexamer: CATAAA"
     polyA_site      1009
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
     regulatory      1632..1637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
                     /note="hexamer: AATAAA"
     polyA_site      1655
                     /gene="Rbx1"
                     /gene_synonym="1500002P15Rik; ROC1"
ORIGIN      
        1 ggaaagtgag ccggtgcgca ggcgcagtgg tcacacgaca gactgtgtgt ttccaaaatg
       61 gcggcggcga tggatgtgga tacccccagc ggcaccaaca gcggcgcggg caagaagcgc
      121 tttgaagtta aaaagtggaa tgcagtggcc ctctgggcct gggacattgt ggttgataac
      181 tgtgccatct gcaggaacca cattatggat ctttgtatcg aatgtcaggc caaccaggcg
      241 tcagctactt ccgaagagtg tacggttgca tggggagtct gcaaccatgc ttttcatttc
      301 cactgcatct ctcgatggct caaaacgagg caggtgtgtc cgttggacaa cagagagtgg
      361 gagttccaga agtatgggca ttaggaaaga ctttcccgca aggcgtaccc atctgttact
      421 cgtctagtga cttcctgtta attatacatt agatagaacc atggtccttt ttcgttcctt
      481 tgtttttgga gtttggtgtt cccgcagcca tattgtattt tgtgtcaaat aaagccttta
      541 agttggaggt ggttgctgtt tcatgtatgt ggagagtgat ctgagaagga gccagaaagc
      601 cagaaaggca gcctcaagaa gtgctctgtt cttaaggaga acacacttgg cccatgggtt
      661 catcttcaca aggtcacgag gaacctgtca gagtcgggag tgcgggtaac tggcacctgg
      721 aaacccctcc agctctgtct tccttctgag atagcaccag gcaggaatga ggaagaacat
      781 ggcagccttg gaccagccag gtctgctctg agttttcatc tcatgatgga gtctaagaac
      841 gagtgactgg catcaagtgg gcacaggaag gctttgtaaa ctctaaacac tacagggtta
      901 gcagtgactg tgaatgacat gcagcaatct ctagaacaaa agttgaccca aaaaatcgta
      961 ctgtgtaatc ttggctggca ataaagtcat cataaagttc acctgaaaac tgatgtttgg
     1021 acagtttgca tatatgcctg taatcccagg tacttggaaa ttaggtaaag atcctttgag
     1081 tccaggagac cttgtttcta aaacagaaat tctagtttgg ggtctagggg gcgctgtagc
     1141 tcagctgtaa ggccccgtgt tcaattgctg gcataaattt caaaaaaaga aaaaggtaga
     1201 aattgaatta gcaagagctt aagttttctt taaacatgct ggccagggcc aggcagtggt
     1261 ggtgcatgcc tttaatccca acacttggga gccagaggca ggcagatttc tgagtttgag
     1321 gccagcctac agagtgagtt tcaggacaac cagggctata taaagaaacc ctgttgccaa
     1381 aaaaacaaaa acatgccggc cagggaagga gcggccatcc tgatgctggt tcagtggctg
     1441 tattctaccg agacaccgtt tctgttgctg tggttttcat gtagttaaca gatcctacaa
     1501 aggtcttgaa atccttcagg ggagttttcc tttctccggt gccactactt caactgtctt
     1561 ctgttgtcaa gcagaaaaag attgcaaaaa tgtgaaacaa tgctgtcatt cttgttttca
     1621 aagttaattt taataaaaat atttatgtta atggc
//
