LOCUS       NM_030081               5301 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus rabenosyn, RAB effector (Rbsn), mRNA.
ACCESSION   NM_030081
VERSION     NM_030081.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5301)
  AUTHORS   Wiese,C.B., Ireland,S., Fleming,N.L., Yu,J., Valerius,M.T.,
            Georgas,K., Chiu,H.S., Brennan,J., Armstrong,J., Little,M.H.,
            McMahon,A.P. and Southard-Smith,E.M.
  TITLE     A genome-wide screen to identify transcription factors expressed in
            pelvic Ganglia of the lower urinary tract
  JOURNAL   Front Neurosci 6, 130 (2012)
   PUBMED   22988430
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5301)
  AUTHORS   Mishra,A., Eathiraj,S., Corvera,S. and Lambright,D.G.
  TITLE     Structural basis for Rab GTPase recognition and endosome tethering
            by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1)
  JOURNAL   Proc Natl Acad Sci U S A 107 (24), 10866-10871 (2010)
   PUBMED   20534488
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY149674.1 and BC049106.1.
            
            On May 29, 2018 this sequence version replaced NM_030081.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049106.1, SRR9219379.57029.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                BY149674.1         2-60
            60-5301             BC049106.1         1-5242
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 41.03 cM"
     gene            1..5301
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="rabenosyn, RAB effector"
                     /db_xref="GeneID:78287"
                     /db_xref="MGI:MGI:1925537"
     exon            1..160
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            161..474
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     CDS             330..2681
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="zinc finger FYVE domain-containing protein 20; FYVE
                     finger-containing Rab5 effector protein rabenosyn-5; RAB
                     effector RBSN"
                     /codon_start=1
                     /product="rabenosyn-5"
                     /protein_id="NP_084357.2"
                     /db_xref="CCDS:CCDS20374.1"
                     /db_xref="GeneID:78287"
                     /db_xref="MGI:MGI:1925537"
                     /translation="MASLDDPGEVREGFLCPLCLKDLQSFYQLQSHYEEEHLEDRDVK
                     GQIKNLVQKARKAKNKLLKREGDDRVEPGTQGYESFSYGGVDPYMWEPQELGAMRSHL
                     SDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTETSKIRAIEKSVVPWVNDQDVP
                     FCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELIGLPLAHKLTSASKDSLSTHTSPSQS
                     PNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDKLLKREQQMDEKEHTPDIVK
                     LYEKLRLCMEKVDQKAPEYIRMAASLNAGETTYNLEHANDLRVEVQKVYELIDALSKK
                     ILTLGLNQDPSPHPNTLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKQDL
                     EQKRTVERQAALESRRKLEERQSGLASHTANGDVRSLRGIPPPLRKAEGWLPLSEGQG
                     QSEDPDPLLQQIYNITSFIRQAKAAGRTDEVRTLQENLRQLQDEYDQQQTEKAIELSR
                     KQAEEEELQREQLQMLRKRELEREQEQFLAASLQTRTRVLELREVIPFQLEASRGPHI
                     DLSYSLDQDSSPVQSSTAPDILTPGSALAPMHLWSGPPALGQETLPQSTMSQQSDKAS
                     LNPFDEDDLSSPTEGAISPAAVEAFLGPPAAVTKEYNPFEEDAEEEEVAELGAGNPFT
                     DPDSPAPNPFDEDDGPRPASPAAPGNPFEECPSTNPFEVDSDSGMEAEEHIEEELLLQ
                     QIDNIKAYIFDAKQCGRMDEVEVLTENLRELKCTLAKQKGAPN"
     exon            475..615
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            616..716
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            717..762
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            763..924
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1166
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1237
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1324
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1427
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1532
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..5279
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4512
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="major polyA site"
     regulatory      4525..4530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="hexamer: AATAAA"
     polyA_site      4544
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="major polyA site"
     regulatory      5214..5219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="hexamer: ATTAAA"
     polyA_site      5234
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
     regulatory      5251..5256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="hexamer: ATTAAA"
     polyA_site      5279
                     /gene="Rbsn"
                     /gene_synonym="5330426D11Rik; Zfyve20"
                     /note="major polyA site"
ORIGIN      
        1 gtcgagcgcc gagccactga tggcgatggc tgccactggt ctctgaggaa cctcgtaggc
       61 cggcagtcag aacccgaggt ggaaaggcag gctgccgact gcctgtcgga agccgcaggg
      121 agtgagaaag ggtgtcgcag agagcgcggc ggtggctgag atttcttctg ctgaagagag
      181 gaaatctcat ccaaggccac aatgtcactt cccaattatc cgagtgctgc ctgctcaagc
      241 taacactgcc agagtcctgc tccctcggga ggagtcaagc ttacttggca tgaggttcct
      301 gctttcctgg ggttgagaga agcacaacta tggcatctct tgatgaccca ggggaggtga
      361 gggaaggctt cctgtgccct ttgtgcctga aggaccttca gtctttctat caacttcagt
      421 cacattatga ggaagaacac ttggaagatc gtgatgtcaa agggcaaatt aaaaatcttg
      481 ttcagaaggc taggaaagca aagaacaagt tgttgaaacg agaaggagat gatcgagtgg
      541 aaccagggac ccaaggatat gaatcattca gttatggtgg ggttgatcct tacatgtggg
      601 aaccccaaga gcttggtgct atgagaagcc atctttctga cttcaaaaaa catcgtgctg
      661 caagaattga ccactatgtt gttgaagtca ataaattaat catcaggttg gaaaagctta
      721 ctgcatttga cagaacaaat actgagactt caaagattag agcaatagaa aagtccgtgg
      781 tgccttgggt caatgaccag gatgtcccct tctgtccaga ctgtgggaat aaattcagca
      841 tccggaaccg ccgtcaccac tgccgcctct gtgggtctat catgtgcaag aagtgtatgg
      901 agctcatcgg cctgccctta gcacataagc tcaccagtgc cagcaaggac tctctgagca
      961 cccacaccag ccccagccag tctcctaaca gtgtccatgg ctcccgccgg ggcagcatca
     1021 gcagcatgag cagtgtcagc tcagtcctag acgagaagga tgatgaccgc atccgctgct
     1081 gcacacactg caaggacaag ctgctcaagc gagagcagca gatggacgag aaggagcaca
     1141 cgcctgacat cgtgaagctc tacgagaaac ttcggctttg catggagaaa gttgaccaga
     1201 aagctcccga atacatcagg atggcagcat ctttaaatgc tggggagacg acctacaatc
     1261 tggagcatgc caatgacctt cgagtggaag tgcagaaagt atatgagcta atagacgctt
     1321 taagtaagaa gatcttaacc ttgggcttga accaagaccc ttcaccacat ccaaacactt
     1381 tgcggctaca gagaatgatc agatactcag ctacattatt tgtgcaggaa aagttactgg
     1441 gtttgatgtc actgccaacc aaagaacagt ttgaagaact gaaaaagaaa aggaagcagg
     1501 acctggagca gaagaggacc gtggagagac aggctgctct ggagtcacgg cggaagcttg
     1561 aggaaaggca gagtggtttg gcatctcata cagccaatgg ggacgtgagg tctcttcgag
     1621 gcatccctcc ccccttgcga aaggctgagg gctggctccc actgtcagaa ggtcagggac
     1681 agagtgaaga tcctgaccct ctcctccagc agatctataa cattacatcg ttcatcaggc
     1741 aggccaaggc tgcaggccgc acagatgagg tgcgcacact tcaggagaac ctgcggcagc
     1801 tgcaggatga gtatgaccag cagcagactg agaaggccat tgagctgtcc cggaagcagg
     1861 ccgaggagga ggagctgcag cgggagcagc tacagatgct ccgcaagcga gagcttgaac
     1921 gagaacagga gcagttcctg gcagcatccc tgcaaacacg gactcgtgtt ctagaactcc
     1981 gagaagttat acccttccaa ttggaggcca gtcgagggcc tcacattgat ctctcctatt
     2041 ccttggatca ggactcctcc ccagttcaga gcagcacagc tcctgatatc cttacacctg
     2101 gctcagctct agcacccatg cacttgtggt ctgggccccc agcccttggc caggaaacac
     2161 tccctcagag caccatgtca cagcaaagtg ataaggcctc actcaacccg tttgatgaag
     2221 acgacctctc cagccctaca gagggtgcta tcagccctgc tgctgtagag gctttcctgg
     2281 gccctccagc tgctgtcacc aaagaataca atccttttga ggaagatgct gaggaagagg
     2341 aggtggcaga gttgggggca ggaaatccct tcaccgaccc agacagtcca gcacccaacc
     2401 cttttgatga ggatgacggt cccaggcctg ccagtccagc cgcccctgga aacccttttg
     2461 aagagtgccc ctccaccaac cccttcgagg tggacagtga tagtggcatg gaggccgagg
     2521 aacacatcga ggaggagctg ctcctgcagc aaattgacaa catcaaggct tacatctttg
     2581 atgccaagca gtgtgggcga atggacgagg ttgaggttct aacggagaac ctccgggagc
     2641 tgaagtgcac cctggctaag cagaaggggg cccctaattg acagcaatgg gcaggaactg
     2701 ggcagagtct tgggcagcag gatatcaagt gtgaggtggg gatgggcagg catctgaaca
     2761 cacacaggtt ggggtgggaa cagtagtctc ttgctgtgca ctttgagaca tttccactac
     2821 agttgagcaa gaacgggaaa aacaatcggc attcatgtgc tggtttttct gtattctttt
     2881 tttaatgaag tattccattt tcaggggact ttaccatttt attttcaaga tacagctctg
     2941 ttgggccaag gcagaaatgg ctcatgggca cctcttagtt cattttctgc ggactgttgt
     3001 tggtcattat gcagtattat agggcagccc ttgacaggcc cttcctgtag ccaggatagg
     3061 cagggctcta gcagaagtct gtcctgactt ggttgttccc tttcagcacg tcacttaggg
     3121 agatactgct gtgaagccca gtgggaccag ggcctcttta tggatggcag gaatgttgcc
     3181 agtgttgtcc cctcccagtt aaaggaagta aaattcccaa gtgtgagggt agtgggctgg
     3241 tggtgtcagt gtgagcttga ctgtgagata gtagtgttca actttttggc ggatgttatg
     3301 gtttggagaa gtttctactt atgcagctgt ctgatcccct gggaacctcc ttccaggtct
     3361 ttggtagaat gaccgttctg caggtcacat tcaagggcct gttgatttgg aattgtgact
     3421 gggaagcttc tgtggagtat tagcaggtga tggaacactt ctgagtcagg atgattctag
     3481 taacttcaag gccctccagt cagccctctt ccttgtggtt tatcctgacc tgggaaactg
     3541 gtaagatgta ctgagaaatg gaaatatttg aggcacagcc tgagaccatt ccacagccct
     3601 gaagtacttg aactcttgat agaaaacaga aagggaagtt atgtagctta gtactttggt
     3661 ttacaggaac agatcgattg acagatgacc ttgtaaggtt cttggtgtgc tcagctctgg
     3721 gtaatttctt tggtgatgat actctttagt taatggacaa taactgtcca ctagtcactt
     3781 tcccctcagc tttctctggg ccatgccttt tgttggttac tggtgtataa gctgaaagtc
     3841 ttctgtcaca ttctggtgaa gatccatcta ggtggtaaga atgttatctg ggtgcaaaga
     3901 tctctatgtt ggcagcttta atcccagttc attagtacat gttggggctt tggctggata
     3961 gagagccctg tatggggcta tgggaagggc tggagaagga gggagcgtat gaaccctttg
     4021 agttctgctg acaggtgttt actggcctgc tagtagatta ccagccattt gccagctctg
     4081 ttggtgtcaa gatgttctgg aaggtaggca gcctggcatt ttatgttacc tggggcaggc
     4141 tacctctttc taaagcccag gctccaggca tgcccaccat atctgccctg cttggtctgc
     4201 tattgtgtca tggacaaaga ggcagtgcta acacaagaat gaagctaaat tctggtttcc
     4261 accattactt ttccagactc aaatcaggtg ttcagggtgg tacaactgta gacaatgctt
     4321 ttcttataag actgggcttc ttaattagct gtgagtgact cgatgggcag tagtggcaca
     4381 tgacttttat cccagcactt gggaagtaga ggcaggaatt tctgagttcc aatccagcct
     4441 ggtctacaga gtgagttcca ggacagccag ggctacacag agaaaccctg tctcaaaaaa
     4501 caaacaaata aataaataaa caaaaataaa aattgagaaa aaaattatct agtgacttat
     4561 taaatgtact tctttctgga actcacccca ggtagcagtc tgactgagag catggagaaa
     4621 acaaatgctt ttagactgac tgactattaa accgggtgaa cccagatccc agagggtttc
     4681 ctgagtttct cattgaattc ctcgctgacc atattctgaa agtccaacag aacacgagag
     4741 acttgtctgc cccttgtctg gatcagctcc cagaatgcat gcaggtcaga gttggtttgc
     4801 agagggggtt tggcctgtga gggaaccaca gtgccagctt cttaccgtgg gtgaatactc
     4861 gatgattccc aaggatggag cacgtccttg atacggtgtt ggcagatccc ttaggtgtag
     4921 gtcctgttca tagtggacag gcttcttgct tattcccttc taacaacact aaggaactgg
     4981 agcctcatca ctgcacttac cacatatcat gtctttgtcg tcagacagta aaacatcttt
     5041 ctaagtcatc atgagccata ttgtgtagct atgccatgcg gttcaaattg tggaattggc
     5101 ctcggtacga ggtcagacta gaggagctcg gtgtgctggt aatcatgatg actgtcagca
     5161 agatggtcat tgatgcgcat gcactacact ggggccttgt attagaggct tttattaaaa
     5221 acattaaaat atcatttatg taaggctttt attaaaaaaa taacatgttc atggctggaa
     5281 aaaaaaaaaa aaaaaaaaaa a
//
