LOCUS       NM_175445               4825 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus Ras association (RalGDS/AF-6) domain family member 2
            (Rassf2), transcript variant 1, mRNA.
ACCESSION   NM_175445
VERSION     NM_175445.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Pan,J., Mor,G., Ju,W., Zhong,J., Luo,X., Aldo,P.B., Zhong,M.,
            Yu,Y., Jenkins,E.C., Brown,W.T. and Zhong,N.
  TITLE     Viral Infection-Induced Differential Expression of LncRNAs
            Associated with Collagen in Mouse Placentas and Amniotic Sacs
  JOURNAL   Am J Reprod Immunol 74 (3), 237-257 (2015)
   PUBMED   26073538
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Song,H., Kim,H., Lee,K., Lee,D.H., Kim,T.S., Song,J.Y., Lee,D.,
            Choi,D., Ko,C.Y., Kim,H.S., Shin,H.I., Choi,J., Park,H., Park,C.,
            Jeong,D. and Lim,D.S.
  TITLE     Ablation of Rassf2 induces bone defects and subsequent
            haematopoietic anomalies in mice
  JOURNAL   EMBO J 31 (5), 1147-1159 (2012)
   PUBMED   22227519
  REMARK    GeneRIF: These observations indicate that RASSF2 regulates
            osteoblast and osteoclast differentiation by inhibiting NF-kappaB
            signalling via association with IkappaB kinase (IKK) alpha and beta
            forms and suppression of IKK activity.
REFERENCE   3  (bases 1 to 4825)
  AUTHORS   Thiagarajan,R.D., Georgas,K.M., Rumballe,B.A., Lesieur,E.,
            Chiu,H.S., Taylor,D., Tang,D.T., Grimmond,S.M. and Little,M.H.
  TITLE     Identification of anchor genes during kidney development defines
            ontological relationships, molecular subcompartments and regulatory
            pathways
  JOURNAL   PLoS One 6 (2), e17286 (2011)
   PUBMED   21386911
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4825)
  AUTHORS   Song,H., Oh,S., Oh,H.J. and Lim,D.S.
  TITLE     Role of the tumor suppressor RASSF2 in regulation of MST1 kinase
            activity
  JOURNAL   Biochem Biophys Res Commun 391 (1), 969-973 (2010)
   PUBMED   19962960
REFERENCE   5  (bases 1 to 4825)
  AUTHORS   Brunskill,E.W., Aronow,B.J., Georgas,K., Rumballe,B.,
            Valerius,M.T., Aronow,J., Kaimal,V., Jegga,A.G., Yu,J.,
            Grimmond,S., McMahon,A.P., Patterson,L.T., Little,M.H. and
            Potter,S.S.
  TITLE     Atlas of gene expression in the developing kidney at microanatomic
            resolution
  JOURNAL   Dev Cell 15 (5), 781-791 (2008)
   PUBMED   19000842
  REMARK    Erratum:[Dev Cell. 2009 Mar;16(3):482. Yu, Jing [added]]
REFERENCE   6  (bases 1 to 4825)
  AUTHORS   MacLaren,E.J., Bennett,B., Johnson,T.E. and Sikela,J.M.
  TITLE     Expression profiling identifies novel candidate genes for ethanol
            sensitivity QTLs
  JOURNAL   Mamm Genome 17 (2), 147-156 (2006)
   PUBMED   16465594
REFERENCE   7  (bases 1 to 4825)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ770520.1, BC057402.1 and AL833794.9.
            
            On Apr 25, 2009 this sequence version replaced NM_175445.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057402.1, AK028384.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               BQ770520.1         1-178
            179-4573            BC057402.1         1-4395
            4574-4574           AL833794.9         191154-191154       c
            4575-4825           BC057402.1         4396-4646
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 64.14 cM"
     gene            1..4825
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /note="Ras association (RalGDS/AF-6) domain family member
                     2"
                     /db_xref="GeneID:215653"
                     /db_xref="MGI:MGI:2442060"
     exon            1..238
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            239..329
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             271..1251
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /note="Ras association (RalGDS/AF-6) domain family 2"
                     /codon_start=1
                     /product="ras association domain-containing protein 2"
                     /protein_id="NP_780654.1"
                     /db_xref="CCDS:CCDS16768.1"
                     /db_xref="GeneID:215653"
                     /db_xref="MGI:MGI:2442060"
                     /translation="MDYTHQPALIPCGQDKYMPKSELLLHLKTYNLYYEGQNLQLRHR
                     EEEDEFIVEGLLNISWGLRRPIRLQMQDDHERIRPPPSSSSWHSGCNLGAQGTTLKPL
                     TMPTVQISEVDMPVEGLETHSPTDSRGLKPVQEDTPQLMRTRSDVGVRRRGNVRTSSD
                     QRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSA
                     EEFALYVVHTSGEKQRLKSSDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYI
                     KFEMPVLKSFIQKLQEEEDREVEKLMRKYTVLRLMIRQRLEEIAETPETI"
     exon            330..405
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            406..557
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            558..646
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            647..807
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            808..909
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            910..961
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1083
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1181
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..4785
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4764..4769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4785
                     /gene="Rassf2"
                     /gene_synonym="3830431H01Rik; 9030412M04Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggtggcgggt gggttggtca aggatcggca gataaaggca gagctcgggg aggcggggcg
       61 gaggggccag gtgggcgggg cgcccgccct caaccgcact gtcgccgccc aggccggccc
      121 aagcgagcgg gtcccgcgga tcactggcca agcacgctgg agcaccccca cccccgcagc
      181 ggcgccccca ctttccaacc cctgcggcga gagcggatgc agatggtccg ggagacaggc
      241 ctctctttct gatgaaaaga ggagggaagg atggactaca ctcaccaacc tgccctaatc
      301 ccatgcggac aagacaagta tatgcccaag agtgaacttc tcctacatct gaagacctac
      361 aacttgtact atgaaggtca aaacctgcaa ctccgacacc gagaggagga agacgagttc
      421 attgtggagg ggttactgaa catctcctgg gggctgcgca ggcccattcg gctgcagatg
      481 caggatgacc acgaacgcat ccgtcctcct ccatcctcct cctcctggca ctccggctgt
      541 aacctgggcg cacagggcac cacactgaag cccctcacca tgcccactgt tcagatctca
      601 gaagtggata tgccagttga aggcctcgag acacacagcc caacagattc cagaggcctg
      661 aagcctgtgc aggaggacac cccacagttg atgcgcacac gcagcgatgt tggggtgcgt
      721 cgccgtggca acgtgaggac atccagtgac cagcgacgga tcagacgcca tcgcttctcc
      781 atcaatggcc atttctataa ccacaagaca tcagtgttca caccagccta tggctctgtc
      841 accaacgtcc gcatcaacag caccatgacc actccgcagg tcctgaagtt gctgctcaac
      901 aagtttaaga ttgagaactc ggcagaggag tttgcgctgt acgtggtcca caccagcggt
      961 gaaaagcaga ggctgaagag ctccgactac cctctgatcg cccgcatcct ccagggccct
     1021 tgtgaacaga tctccaaagt tttcctgatg gagaaggacc aggtggagga ggtcacctat
     1081 gacgtggccc agtatataaa gttcgagatg ccagtcctta aaagcttcat tcagaaactc
     1141 caggaggagg aagaccggga ggtggagaag ctgatgcgca agtacactgt gctccggcta
     1201 atgattcggc aaaggctcga ggagatagca gagacccccg agactatctg agccacagga
     1261 atgaagacag tccagacact ccaggggccg aggctgacct gagaagaccc actggaagct
     1321 aggcatccta cagaaacgtt ttcacacgat ccttgcagcc ttccaaaagc cagcactgct
     1381 ccctacagta ggacacagaa gcagcagaca gctccccacc tttggacccc tgatcttctt
     1441 tgtctcattc tcaaggactt ttgtcctaag gaggacccac aatgtgtatg cttgcatacc
     1501 tgagtgtgtg tatgtgtatg tgcacgcata cgcaagtgtg catatgtgta cttaccatgt
     1561 ccacgtgcct ctctgatgtt cttacatttg gttaaattcc cagcagccag caggtgtcac
     1621 aaggcaggag ggcgcacgtg ctgctcctgt gtgggaagaa cagggaagtg actgaggctt
     1681 ttggcttcct ccagtgccaa cggcagccgc acggatctgc cacgcttgac agggaagagg
     1741 aggcagcagg ctcaccgttt ggaatgtctg aaggtttaca gcagagattc ttctcatttg
     1801 ccaaggggtg gccccatgcc cttggggcaa gtccaacgaa atggaaaaag caagatcacc
     1861 tcagggcgga tgcaatgaat ctatgagttt ttgcactgga aaggttatca atgtagaaag
     1921 cacaattaat gtccccgtcc tggatggaaa cctagaaaga atggattctg ccttcgttct
     1981 tggctttgtg gaggcttctg ggtgtttctg ctctccagct gccacttcaa ttcaccctga
     2041 cctgctcatg tgcctgggcc aggtgtgtgc tgggataaac ggcactcgaa agtttttaag
     2101 ccaccagctg agatgagagg tacctttaag cctagcccac catcacatcg gtgagagtat
     2161 ctcccacgtg gaggtggagc agtgtgtttg aagtcgcatg tctttctttc tggtctctgc
     2221 acacttgcaa gctccttcac ctgatacacg gagatggatt ccaagaatgg agacctgtcc
     2281 tgttgctcag tcaacacagg gagtcaggaa gctctttggg gtgttccatg agggtgggca
     2341 gagaggacgg tgcttgctta gcctttgaga agccatgctc ccgattttca gaaccagcct
     2401 cattggcaga aagggcaggg atgatcctgt aacccctcca ttcgggatgc tgtcatgtag
     2461 ctggtcacct gtgtggcttg taccatttga gtttggacgg atggatgggc ttgctgctgg
     2521 tctatgaaac tcagccaagc aaggggagca gcccataact caagcatctc tgagttttgc
     2581 tacaacaggg tgttcttgtc tacctgctct caagaaagct ctagaagcac agcagcggag
     2641 agactgaggg gccccgacca gtgcgccttg taaggaaggt ggacctctgc agcagatctg
     2701 ttctagtgta ggaatgttac tgtcctttgc ccttctcaga ggctcacagc tgtgatatca
     2761 ttgtgggctt tcaaacagat ggctcatggc cagccatttt gctgggtatc acttagcaac
     2821 cttgctcttc ttctaggaca gcacaatcca tcccatgagc agtgggatgg aaacctgtgg
     2881 gtctgagtct ttatcttaga ctcaaaagac agcaaagcac agaactgagc acagatactg
     2941 agtgtatctt tatgagtaca cacttcagta aactctgagc tcagtgggca gctggggtgg
     3001 gggttccttt atgacagaca aagaacagct ttgtgaatgc aaatacttga gaaaatttgg
     3061 tttggataac ttctcttccc gttatcttta tcttgaatgt tcagagccta tgttggtcca
     3121 caattctccc ttgggagcct gggggctttc catggagcac acgaactggg aagttatttc
     3181 tgatttatga aagctcattg tgtaagtgtc aatatgtgac tgttgctggg catcgggttg
     3241 ccaggctttc caagatggtg gtgccaagat agacagcttt taaagactgg ggttcagaaa
     3301 gggagttggg gtacatgttc actaatccat cttggcacat tccctagaga tcatgcctta
     3361 tgtccaaaga actgggcagg agttttagga aattggctgg gaatgagact cagtggtgga
     3421 acattttctt agtgtgtgtg aaaagccgac ttcaatctct agaaacacac acacacacac
     3481 acacacacac acacacacac acacacacac acacacacac atgcacgcat gcacgcacgc
     3541 acgcagcagt ggtgtagctc agtgatagag ggattgctta gagtgtcaga ggctggttcc
     3601 cgacgcctgc accaagcacc acactacccc ctgcactttg gaagcctttg acatagccgc
     3661 agcagaacag acctctacag tgacgactgg attttctcac tggtctcact tcacagcttt
     3721 gaactccaag actgattagt gtgggcttat gtaattgggt gcaatggacc tgaaatcctg
     3781 agaaataaaa gactaacgca taaggcggcg gtgggggtgc taatccagca tgtgaaaagt
     3841 ggttcggctg cctctggact cttcccctcc cccaccccca cccccacaaa ggcttatttt
     3901 acaaggtgat caagtgccga gaacaccagc ctccgcctga gcgaggtggt gaggccaagc
     3961 acaggttgac tttctcggtg accatgttag cggggagctg atgcctcaca gtgggtgggg
     4021 gtgttagttg gaaaggggcc caaagcacaa acacccgtca tgcctgtgct actggtgaag
     4081 ggcacacagc taacaccatg gacaaacgca gctttgtaac gtggaaagtt tggggttagg
     4141 gatacatttc caagaaagaa atatgtccag tgaccaaagg agttttaatg tcccacagga
     4201 gtcagctttg ccctggtgag ggaggaggta ggaggctgcc ctagctcacc cccacagcac
     4261 tgcctatctt aaagcatatc acctaggttg ttttctggaa agcctggctt ccgccggatg
     4321 agagagcatc tatgaacctg atgtgacatc ccttccttca gacataattg acagtggttg
     4381 ccattccttg gggtacacct tcctcccttc ctcctgctcc cattaaactg agcacagagg
     4441 tgcttgaact gtgaactttg gagaagcctc agtctccagg ttgctggatt ctggggttgc
     4501 tgggttctgg ggttactact agtgtttctt cgggtttttt cttagttgag tggagtcttt
     4561 tttttttttt ttttcttcat cataacttgc ctgttaggaa tggtacatga cttggcttaa
     4621 ctgttggtta tgaactgaaa atcggatgtg cttaggaatc ttaacatgat tggatgggac
     4681 cttgatgggg agccatggtt ttgtagcctt ttatttttta attattattt tttttgtaat
     4741 gtatccatct tagaccttat ttaaataaag cacttttgga agcgaaaaaa aaaaaaaaaa
     4801 aaaaaaaaaa aaaaaaaaaa aaaaa
//
