LOCUS       NM_145149               4621 bp    mRNA    linear   ROD 20-SEP-2024
DEFINITION  Mus musculus RAS guanyl releasing protein 4 (Rasgrp4), transcript
            variant 2, mRNA.
ACCESSION   NM_145149
VERSION     NM_145149.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Suire,S., Baltanas,F.C., Segonds-Pichon,A., Davidson,K., Santos,E.,
            Hawkins,P.T. and Stephens,L.R.
  TITLE     Frontline Science: TNF-alpha and GM-CSF1 priming augments the role
            of SOS1/2 in driving activation of Ras, PI3K-gamma, and neutrophil
            proinflammatory responses
  JOURNAL   J Leukoc Biol 106 (4), 815-822 (2019)
   PUBMED   30720883
REFERENCE   3  (bases 1 to 4621)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4621)
  AUTHORS   Zhou,S., Tanaka,K., O'Keeffe,M., Qi,M., El-Assaad,F., Weaver,J.C.,
            Chen,G., Weatherall,C., Wang,Y., Giannakopoulos,B., Chen,L., Yu,D.,
            Hamilton,M.J., Wensing,L.A., Stevens,R.L. and Krilis,S.A.
  TITLE     CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to
            Function as Accessory Cells for Optimal Natural Killer
            Cell-Mediated Responses to Lipopolysaccharide
  JOURNAL   PLoS One 11 (3), e0151638 (2016)
   PUBMED   26982501
  REMARK    GeneRIF: RasGRP4 is required for CD117+ MCs and DCs to optimally
            induce acute NK cell-dependent immune responses to LPS helps
            explain why this signaling protein has been conserved in evolution
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4621)
  AUTHORS   Fischl,A.M., Heron,P.M., Stromberg,A.J. and McClintock,T.S.
  TITLE     Activity-dependent genes in mouse olfactory sensory neurons
  JOURNAL   Chem Senses 39 (5), 439-449 (2014)
   PUBMED   24692514
  REMARK    GeneRIF: Rasgrp4 is an activity dependent gene in mouse olfactory
            sensory neurons. [Rasgrp4]
REFERENCE   6  (bases 1 to 4621)
  AUTHORS   Ikuta,S., Edamatsu,H., Li,M., Hu,L. and Kataoka,T.
  TITLE     Crucial role of phospholipase C epsilon in skin inflammation
            induced by tumor-promoting phorbol ester
  JOURNAL   Cancer Res 68 (1), 64-72 (2008)
   PUBMED   18172297
REFERENCE   7  (bases 1 to 4621)
  AUTHORS   Johnson,J.E., Goulding,R.E., Ding,Z., Partovi,A., Anthony,K.V.,
            Beaulieu,N., Tazmini,G., Cornell,R.B. and Kay,R.J.
  TITLE     Differential membrane binding and diacylglycerol recognition by C1
            domains of RasGRPs
  JOURNAL   Biochem J 406 (2), 223-236 (2007)
   PUBMED   17523924
REFERENCE   8  (bases 1 to 4621)
  AUTHORS   Stevens,R.L., Morokawa,N., Wang,J. and Krilis,S.A.
  TITLE     RasGRP4 in mast cell signalling and disease susceptibility
  JOURNAL   Novartis Found Symp 271, 54-68 (2005)
   PUBMED   16605128
  REMARK    Review article
REFERENCE   9  (bases 1 to 4621)
  AUTHORS   Li,L., Yang,Y., Wong,G.W. and Stevens,R.L.
  TITLE     Mast cells in airway hyporesponsive C3H/HeJ mice express a unique
            isoform of the signaling protein Ras guanine nucleotide releasing
            protein 4 that is unresponsive to diacylglycerol and phorbol esters
  JOURNAL   J Immunol 171 (1), 390-397 (2003)
   PUBMED   12817022
REFERENCE   10 (bases 1 to 4621)
  AUTHORS   Yang,Y., Li,L., Wong,G.W., Krilis,S.A., Madhusudhan,M.S., Sali,A.
            and Stevens,R.L.
  TITLE     RasGRP4, a new mast cell-restricted Ras guanine
            nucleotide-releasing protein with calcium- and
            diacylglycerol-binding motifs. Identification of defective variants
            of this signaling protein in asthma, mastocytosis, and mast cell
            leukemia patients and demonstration of the importance of RasGRP4 in
            mast cell development and function
  JOURNAL   J Biol Chem 277 (28), 25756-25774 (2002)
   PUBMED   11956218
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY749980.1, AK049562.1 and AC164564.3.
            
            On Mar 31, 2010 this sequence version replaced NM_145149.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK089282.1, AY040628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-257               BY749980.1         2-258
            258-3168            AK049562.1         1-2911
            3169-4621           AC164564.3         104555-106007       c
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.94 cM"
     gene            1..4621
                     /gene="Rasgrp4"
                     /note="RAS guanyl releasing protein 4"
                     /db_xref="GeneID:233046"
                     /db_xref="MGI:MGI:2386851"
     exon            1..163
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..2162
                     /gene="Rasgrp4"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="RAS guanyl-releasing protein 4 isoform 2"
                     /protein_id="NP_660131.2"
                     /db_xref="CCDS:CCDS85257.1"
                     /db_xref="GeneID:233046"
                     /db_xref="MGI:MGI:2386851"
                     /translation="MNRKDIKRKSHQECSGKAGGRGRSRQARRHKTCPTPREISKVMA
                     SMNLGVLSESSCSEDELLEECIRCFDSAGSLRRGDHILKMVLTMHSWVLPSSELAARL
                     LTSYQKAAKDAQELRQLQICYLVRYWLTHHHEAVHQEPQLEAVISRFWTTVAQEGNMA
                     QRSLGDASSLLSPGGPGPPPPMSSPGLGKKRKVSLLFDHLETEELAQHLTYLEFRSFQ
                     AITPQDLRGYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIR
                     VAQRLHQLQNFNTLMAVTGGLCHSAISRLKDSHVHLSPDSTKALLELTELLSSHNNYA
                     HYRRTWAGCTGFRLPVLGVHLKDLVSLYEAHPDRLPDGRLHLPKLNSLYLRLQELMAL
                     QGQHPPCSANEDLLHLLTLSLDLFYTEDEIYELSYAREPRCPKSLPPSPFKAPVVVEW
                     AQGVTPKPDSVTLGQHVEQLVESVFKNYDPEGRGSISLEDFERLSGNFPFACHGLHPP
                     PRHGSGSFSREELTKYLLHASAICSKLGLAFLHAFQEVTFRKPTFCHSCSGFLWGVTK
                     QGYRCRDCGLCCHRHCRDQVRVECKKRPETKGDPGPPGAPVPATSLPPANCGSEESLS
                     YTLSPDPESGCHLRHAWTQTESSHSSWEPEVVPCPARVLPSRASSKPSV"
     exon            164..348
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            349..454
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            455..517
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            518..649
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            650..803
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            804..977
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1094
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1370
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1451
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1556
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1675
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1820
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1857
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1992
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2105
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..4621
                     /gene="Rasgrp4"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2281
                     /gene="Rasgrp4"
                     /note="major polyA site"
     regulatory      3184..3189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasgrp4"
                     /note="hexamer: ACTAAA"
     polyA_site      3205
                     /gene="Rasgrp4"
ORIGIN      
        1 agttcctctt tccttcctaa actcggtgga cgctccagct ccccggagcc tgacaatctg
       61 gttagggaag gaacctgatc acactcctgt caggagcccc caccccaccc ccccgaccct
      121 ccccatcccc tccaagaaac atgaaccgga aagacatcaa aaggaagtct catcaggaat
      181 gctctgggaa ggcaggaggg cggggtcggt cacgtcaggc ccgccgccac aagacgtgcc
      241 ccaccccccg ggaaatcagc aaggtcatgg cgtccatgaa tctgggagtg ctgagtgaga
      301 gcagctgcag cgaagatgag ctattggagg aatgtatccg ctgctttgac tcggctggca
      361 gcctgcgccg aggggaccac attctcaaga tggtgctcac gatgcacagc tgggtgctgc
      421 catcctcaga gctcgctgcc cgtctgctga cttcgtatca gaaggctgcc aaagatgccc
      481 aagagctaag acagctacag atctgttact tggtcaggta ctggctgacc catcaccatg
      541 aggcagtgca ccaggaaccc cagctggaag cagtcataag ccggttttgg accactgtcg
      601 ctcaggaggg caacatggcc caaaggagcc tgggagatgc ctccagcctg ctaagtcctg
      661 gagggcccgg tcccccacct cccatgagca gcccaggcct gggcaagaag cggaaagtgt
      721 cattgctatt cgatcacctg gagacagagg agctggcaca acacctcact tacctggagt
      781 tccggtcttt ccaggctatc acaccccaag acctgcgggg ctatgttttg cagggttcag
      841 tgagaggttg tccagctctg gaaggttctg tgggtctgag caacagtgtg tcccgctggg
      901 tgcaggtcat ggtgctgagt cgtcctgggc ctgcacaacg tgcccaggtc ctggacaaat
      961 tcattcgtgt ggcacagagg cttcaccagc tgcagaattt caacacactg atggcggtca
     1021 cggggggtct gtgtcatagc gccatctcca gactcaagga ctcccacgtt catctgagcc
     1081 ctgacagcac aaaggccctg ctggagctga cagagctcct ctcatcccac aacaactatg
     1141 cccactaccg ccgcacctgg gccggctgta ctggcttccg gctgccagta ctgggtgtgc
     1201 acctcaagga cctggtatct ctatatgagg ctcatccaga cagattgcca gatggccgcc
     1261 tgcacctacc caagctgaat agcctctatc tacggctgca ggagctgatg gcgctccagg
     1321 gacagcatcc tccctgcagt gccaatgagg acctgctgca cctgctgacg ctctccctgg
     1381 atctcttcta cacggaagat gagatctacg agctttctta tgcccgggaa ccacgctgtc
     1441 ccaagagtct gccaccgtcc cccttcaaag caccagtggt ggtagagtgg gcccagggtg
     1501 tgacaccaaa gccagacagc gtgactctgg gtcagcatgt ggaacagctg gtggagtccg
     1561 tgttcaagaa ctacgaccca gaaggccgtg gctccatctc tctggaggac tttgagcggc
     1621 tgtcaggcaa cttcccgttc gcctgccatg ggcttcaccc acctccccgc catgggagtg
     1681 gctccttcag cagagaggag ctgaccaagt acctgctcca tgccagtgcc atctgctcca
     1741 agctgggcct ggccttcctg cacgccttcc aggaggtcac cttccgaaag cccacgttct
     1801 gtcacagctg cagcggcttc ctctgggggg tcaccaagca aggctatcgc tgtcgggact
     1861 gtgggctgtg ttgtcacaga cactgcaggg atcaagtgag agtggagtgt aagaagaggc
     1921 cagagactaa gggtgacccg ggccccccag gtgcacctgt gccagccaca tcacttcctc
     1981 ctgccaactg tggctcagag gaaagtctct cctatacact ctccccggat ccggagtctg
     2041 gttgccacct tcgccatgct tggacccaga cggaatcctc acactcttcc tgggagccag
     2101 aggtggtgcc ctgcccagca cgggtcttac catccagagc ttcctcgaag cccagcgtct
     2161 gaagagcatc tggaggcttt tcccttactc ccctaaccaa cgcctcctcc acccccactc
     2221 tgcctctgac aaagcaccca ccatcttttc ccctaaaaaa aagccgttct tttgcaccgc
     2281 actgactgcc agtcacactc cttcccctca cttttatctg tagctctgtg ctggatgcac
     2341 ttgggctcca gctgtgtgct ccccagctcc ccacatccga ggaggaagac tcagtaaggc
     2401 tgaccttcaa gcccaggtgt gggaggcaag actcgcctgt tctaaaactg gagctctgag
     2461 ccagcggcct gcacgggctg ttggcatggg cttgggaggc tgtgagcgtg gaggggacag
     2521 ggttctaacc agtgccagaa aaggggctgg tgtgggcagt gtgtgaaaaa catcttcctt
     2581 tgcttgctca aggtaaaacc cagtcctatg gtttacaaag ctctgcaaga tggctcctca
     2641 ccacctgtct tcccttccca cagctcacct ttcctcatcc tcttccacct acactggcca
     2701 ctgggttcct agaacatgcc ccaaatactc ctaccccagg gcctttgcac tgccggttcc
     2761 ctccctttac ctgcaatgct ccttgccaga ggtctgtatg acttacctgc tctagaactt
     2821 tgctccagtg atgtcccctt gggcggggag ggggggtggg gctcctgacc gcaagacttg
     2881 tcacacagac atgttctgaa cccctctccc ttctgcttta tttctctctg gcactttggt
     2941 cctctaacaa gcgagagatt cactatttat cttgttaaat gtctgcccct ccttgccagg
     3001 aaccactccc caaaggcaga gatgtttatc tctattatct agctgtgcct agatagacta
     3061 cctggctgag ttggtaccag ggagcaccta taaagtccga tgcttcaatg tgtcttttcc
     3121 cacaaaccca tacacccctg acctggtccc taacagaaac aagatcctat agccatcaaa
     3181 agaactaaat ttatttttca gacaacacgg ttcatagtgt tgagtggcgg ctgggtgtag
     3241 ggcatttagg gcctctagaa tagggaaggc caggcttgcc gaggataacc cctcagtgcc
     3301 tcctgaagga gtgccagcag catcagcgcc tggcagaatc tgcaaccgtc cctcaaaggc
     3361 ccttcacttc ttcttctttc tgcggcccca gcgctgttgg ccagccttcc cccttcctcc
     3421 atcttctcgt cggctcttcc aagtaggcat gggagcttcc agctcactgg ggcgcccccc
     3481 acggtctggc acatcaccca taagcaccta agagttgata agacttgagt ttcagtagga
     3541 ggtaaagaga gctgggggtg ctggtgtccc ccagcatcct atcacttggg aaatggaaga
     3601 gtttataccc agcctttggc tacataatga gttggaggct agcctgggct acctaggact
     3661 ctatatctca aagggggggg gcggggagga aggtgtgacg gtggtacata cttttaatct
     3721 tggcactcag gaggcagtgg caggaggatc tctgagttaa gaggccaccc tggtctacag
     3781 agttccagga cagtaaaagc tacacaggga agccctgtct cagaaaaaaa aatctttcta
     3841 gctgacatgc agagtaggta gtatgaattc agttgagtcc agaggccagg aaccaaggag
     3901 aacactgaac cagggaaacc cccaaaagac caatctaaaa gccaaggatg cccagactct
     3961 ccccgcccca ggtgtgccca ccttgttgat tgcacaactg gtccctcgtc gggcagccac
     4021 actggtagct gggaccaggc gggaaaggtc agctcgggca gccagaacat gtgcgataga
     4081 gacatcagat tctggagtca gaagccctcg gattggggac agcaccgcct tcatgacctc
     4141 tccgtgggcc tagagcagga gaagggcata cacggagaag atgcccaaga gctcatccct
     4201 actcagggcc tccgtgtgct ttggagggag tgggtgcttc tccaaggtgc atgtgctgtg
     4261 gtggagagct ggatgcgagt aaccctggag tctcagcact agggaagagg gagggtcagg
     4321 agggactgtc aagatcagcc tcagctacct cgcaagttga agcagaaaca aaatctcaga
     4381 aggggttgac catgtgagaa ctagagcctg gccgacaatc acggggtacc accatcggag
     4441 ggattcctgc aagtcctatt gtgttatggt gacatgggat gggacattgg ccagcacctg
     4501 tccaaggcta catgtttcag cctccaaaac cataagccaa aaggaacttg tttttcctga
     4561 aaaaaactct gggtttctgg tattttctgt catggctaca caaaaacagt tttaaaaaaa
     4621 a
//
