LOCUS       NM_011245               4243 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus RAS protein-specific guanine nucleotide-releasing
            factor 1 (Rasgrf1), transcript variant 1, mRNA.
ACCESSION   NM_011245
VERSION     NM_011245.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Zhu,L.Y., Yuan,J.B., Zhang,L., He,C.X., Lin,X., Xu,B. and Jin,G.H.
  TITLE     Loss of MLL Induces Epigenetic Dysregulation of Rasgrf1 to
            Attenuate Kras-Driven Lung Tumorigenesis
  JOURNAL   Cancer Res 82 (22), 4153-4163 (2022)
   PUBMED   36098964
  REMARK    GeneRIF: Loss of MLL Induces Epigenetic Dysregulation of Rasgrf1 to
            Attenuate Kras-Driven Lung Tumorigenesis.
REFERENCE   3  (bases 1 to 4243)
  AUTHORS   Choi,S.Y., Ahn,Y.R., Lee,E.B., Yu,M.J. and Lee,J.R.
  TITLE     Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor,
            p190RhoGEF, in Mouse Macrophages Negatively Affects M1 Polarization
            and Inflammatory Responses
  JOURNAL   Front Immunol 13, 782475 (2022)
   PUBMED   35422804
  REMARK    GeneRIF: Expression of a RhoA-Specific Guanine Nucleotide Exchange
            Factor, p190RhoGEF, in Mouse Macrophages Negatively Affects M1
            Polarization and Inflammatory Responses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4243)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4243)
  AUTHORS   Jiang,W., Shi,J., Zhao,J., Wang,Q., Cong,D., Chen,F., Zhang,Y.,
            Liu,Y., Zhao,J., Chen,Q., Gu,L., Zhou,W., Wang,C., Fang,Z.,
            Geng,S., Xie,W., Chen,L.N., Yang,Y., Bai,Y., Lin,H. and Li,X.
  TITLE     ZFP57 dictates allelic expression switch of target imprinted genes
  JOURNAL   Proc Natl Acad Sci U S A 118 (5) (2021)
   PUBMED   33500348
REFERENCE   6  (bases 1 to 4243)
  AUTHORS   Gariboldi,M., Sturani,E., Canzian,F., De Gregorio,L., Manenti,G.,
            Dragani,T.A. and Pierotti,M.A.
  TITLE     Genetic mapping of the mouse CDC25Mm gene, a ras-specific guanine
            nucleotide-releasing factor, to chromosome 9
  JOURNAL   Genomics 21 (2), 451-453 (1994)
   PUBMED   8088844
REFERENCE   7  (bases 1 to 4243)
  AUTHORS   Cen,H., Papageorge,A.G., Vass,W.C., Zhang,K.E. and Lowy,D.R.
  TITLE     Regulated and constitutive activity by CDC25Mm (GRF), a
            Ras-specific exchange factor
  JOURNAL   Mol Cell Biol 13 (12), 7718-7724 (1993)
   PUBMED   8246988
REFERENCE   8  (bases 1 to 4243)
  AUTHORS   Cen,H., Papageorge,A.G., Zippel,R., Lowy,D.R. and Zhang,K.
  TITLE     Isolation of multiple mouse cDNAs with coding homology to
            Saccharomyces cerevisiae CDC25: identification of a region related
            to Bcr, Vav, Dbl and CDC24
  JOURNAL   EMBO J 11 (11), 4007-4015 (1992)
   PUBMED   1396590
REFERENCE   9  (bases 1 to 4243)
  AUTHORS   Wei,W., Mosteller,R.D., Sanyal,P., Gonzales,E., McKinney,D.,
            Dasgupta,C., Li,P., Liu,B.X. and Broek,D.
  TITLE     Identification of a mammalian gene structurally and functionally
            related to the CDC25 gene of Saccharomyces cerevisiae
  JOURNAL   Proc Natl Acad Sci U S A 89 (15), 7100-7104 (1992)
   PUBMED   1379731
REFERENCE   10 (bases 1 to 4243)
  AUTHORS   Martegani,E., Vanoni,M., Zippel,R., Coccetti,P., Brambilla,R.,
            Ferrari,C., Sturani,E. and Alberghina,L.
  TITLE     Cloning by functional complementation of a mouse cDNA encoding a
            homologue of CDC25, a Saccharomyces cerevisiae RAS activator
  JOURNAL   EMBO J 11 (6), 2151-2157 (1992)
   PUBMED   1376246
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102545.9, AK140380.1, AK079638.1 and CD775453.1.
            
            On Mar 1, 2006 this sequence version replaced NM_011245.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC138683.1, AK140380.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-419               AC102545.9         113987-114405
            420-2061            AK140380.1         287-1928
            2062-2310           AK079638.1         1945-2193
            2311-3573           AK140380.1         2178-3440
            3574-4182           AK079638.1         3457-4065
            4183-4243           CD775453.1         1-61                c
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 47.31 cM"
     gene            1..4243
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /note="RAS protein-specific guanine nucleotide-releasing
                     factor 1"
                     /db_xref="GeneID:19417"
                     /db_xref="MGI:MGI:99694"
     exon            1..499
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..4012
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ras-specific guanine nucleotide-releasing factor 1;
                     ras-specific nucleotide exchange factor CDC25; Ras guanine
                     release factor beta"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     1 isoform 1"
                     /protein_id="NP_035375.1"
                     /db_xref="CCDS:CCDS40722.1"
                     /db_xref="GeneID:19417"
                     /db_xref="MGI:MGI:99694"
                     /translation="MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWF
                     ALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSN
                     DSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQ
                     LRQQLEDGEVEIERLKTEVTITNLIKDNDRIQSSNKAGSADDEDSDIKKIKKVQSFLR
                     GWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRM
                     AASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPML
                     NIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRY
                     ILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEG
                     CEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKKEGERQCFLFSKHLIICTR
                     GSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSLPFTV
                     ILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVD
                     IRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKL
                     INIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSR
                     RRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKT
                     PEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDEDNTEVSPVKSPPTPKSF
                     LNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRR
                     MSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFL
                     EEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALE
                     IAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIR
                     NEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKS
                     LLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNF
                     SKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT
                     "
     exon            500..609
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..763
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            764..859
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1113
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1193
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1387
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1497
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1616
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1777
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1841
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..1978
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2061
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2310
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2687
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2810
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2811..2984
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2985..3091
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3092..3204
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3205..3265
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3266..3369
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..3454
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3455..3652
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3653..3732
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3850
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3851..3919
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3920..4228
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4205..4210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /note="hexamer: AATATA"
     polyA_site      4226
                     /gene="Rasgrf1"
                     /gene_synonym="CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190;
                     P190-A; p190RhoGEF; Ras-GRF1"
                     /note="major polyA site"
ORIGIN      
        1 gcgagcacgg cgcccgatct caaagtgtag atcttgaagg gggcggggtg tgagagagcg
       61 agggagggcg ggggagcgag agagagagag agagcgcgcg cgagggagag cgagggagcg
      121 attcagagag cgccagagaa cggcagagcg cgcaccgacg aggggcattc ggatgcctct
      181 ctgagatgta agtgcgtagc agtctctcag gccttctagc atcatgcaga aagccatccg
      241 gcttaacgat ggccacgtcg tgaccctggg actgctggca cagaaggacg gcacgcgcaa
      301 aggctacctg agcaagagga gtgcggacaa cccaaaatgg caaaccaagt ggtttgccct
      361 gctccagaac ctgcttttct acttcgaaag cgactccagc cctcggccct cggggctcta
      421 cctgctcgag ggaagtatct gtaaacgcgc gccctccccc aagcgaggga cctcctccaa
      481 ggagtccggc gagaaacagc aacattactt cacagtgaac ttctccaatg acagccagaa
      541 gaccttggag ttgaggaccg aggatgccaa ggactgtgac gagtgggtgg ctgcgatcgc
      601 tcgcgccagc tacaagatac tggctacaga acatgaggcg cttatgcaga agtacttaca
      661 cctgctgcag gtagtggaga cagagaagac tgtggctaag cagctgcgcc agcagcttga
      721 ggacggtgag gttgagatcg agcgcctgaa gacggaggta actattacaa acctgatcaa
      781 ggacaatgat cgtatccaat ccagcaacaa agccggttct gctgatgatg aggacagtga
      841 catcaagaaa attaagaagg ttcagagctt ccttcgggga tggctgtgcc ggcgaaagtg
      901 gaagaacatc atccaggact acatccggtc tccccacgcg gacagcatgc gcaagaggaa
      961 ccaggtggtg ttcagcatgc tggaggccga ggctgagtac gtccagcagc tacacatcct
     1021 tgtcaacaac ttcctgcgcc cactgcgcat ggccgccagc tctaagaaac cccctataac
     1081 acacgacgac gtcagcagta tctttctgaa cagcgagacc atcatgttcc tgcatcagat
     1141 cttctaccaa ggcctgaagg cccgtatctc cagctggccc accctggtgc tggcggacct
     1201 gttcgatatc ctgctgccca tgctgaacat ctaccaggag ttcgtccgca accaccagta
     1261 cagcctccag atccttgctc actgcaagca aaaccgggac tttgacaagc tcctcaagca
     1321 gtacgaggcc aagccagact gcgaggagcg cacactggag accttcctca cctacccaat
     1381 gttccagatc cccaggtaca tcctgacact ccatgagctg ctggcccaca cacctcatga
     1441 gcatgtggag cgcaacagcc tggactatgc caaatccaaa ctagaggaac tgtccaggat
     1501 catgcatgac gaagtcagtg aaaccgagaa catccgcaaa aacctggcca ttgagcgtat
     1561 gatcacggag ggctgtgaga tcctcctgga caccagccag accttcgtgc gccaaggttc
     1621 cctcatgcag atgtccttgt ctgaaaagag caagtcctct aggggccgcc tggggtctct
     1681 gtccactaag aaagagggtg agcgccagtg tttcctattc tccaagcatc tcattatctg
     1741 caccagaggc tctggtggga aactgcacct aaccaagaat ggagtcatat ccctcattga
     1801 ctgcacctta ctggatgaac ccgaaaacct ggacgacgaa gccaaaggag ccggaccaga
     1861 gatagaacac ctggagttta agatcggggt ggagccaaag gactccctac ccttcactgt
     1921 catcctggta gcctcaacca ggcaggagaa ggcagcatgg accagtgaca tcatccagtg
     1981 cgtggataat atccgctgca atgggctcat gatgaatgcc tttgaagaaa attccaaggt
     2041 caccgtgcct cagatgatca agtctgacgc ttccttatat tgtgatgatg ttgacattcg
     2101 cttcagcaaa accatgaatt cttgcaaagt gcttcagatc cgctatgcca gcgtggagcg
     2161 cctgctggag cgcctgactg atcttcgctt cctgagcatt gactttctca acaccttcct
     2221 gcattcctat cgcgtcttca ccaatgctat ggtggtcctg gacaagctga tcaatatcta
     2281 cagaaaaccc atgagtgcga ttcctgccag gtccctggaa ctcttgtttt ccagcagcca
     2341 caacgccaag cttctgtacg gagatgcccc taagtcccct cgcgccagcc gcaagttctc
     2401 ctcgccaccg cccttggcca tcgggacgtc atcccccagc cgccgtcgga agctgtccct
     2461 caacatcccc atcatcacag gcggcaaggc gctggaactg gcttcccttg ggtgcagctc
     2521 cgatagctac gccaacatac actcacccat atctcccttc ggcaaaacca cactggacac
     2581 tggcaagctc tgtatggcca gcagcttacc caagactcca gaggagattg atgtgcctgc
     2641 cacgattcca gagaagcctg gggagctctc agcctccagg aagcatagct ctgatgtcct
     2701 gaaagaagag tccgaggatg accagaacca cagtgatgaa gacaacacag aagtgtcacc
     2761 agttaaatcc ccaccaacac caaaatcgtt cttaaacaga accattacag agtttccatt
     2821 ctttaactac aacaatggaa tcctgatgac cacgtgtcgt gatctggtgg acaataaccg
     2881 cagcactctc tcagctactt ctgcctttgc catagcgact gcaggagcca atgaaggccc
     2941 ctcaaacaag gaggtgtttc gaaggatgtc tttggccaac acaggctttt cctctgacca
     3001 gagaaatatc gacaaggagt ttgtgatccg cagagctgcc accaatcgtg tgctgaatgt
     3061 gctccgccac tgggtcacca agcacaccca ggactttgat accgacgata cgctcaaata
     3121 cagggtgatc tgtttcctgg aggaggtcat gcatgaccca gaccttctga cccaagagcg
     3181 aaaggcagca gccaacatca tcaggactct gaccctagaa gaaacaactg aacaacatag
     3241 catgctggaa gaagtcatac tgatgacgga gggtgtgaag actgagccct tcgaaaacca
     3301 cccagccctg gagatagcag agcagctgac cctgctggat caccttgtct tcaagagtat
     3361 tccttatgaa gagttctttg gccagggctg gatgaaggcc gagaagtatg aaaggacacc
     3421 ttacattatg aaaaccacca aacacttcaa ccatgtcagt aacttcattg cttcagaaat
     3481 tatccgaaac gaggacatca gcgcaagggc aagcgccatc gagaagtggg tggctgtggc
     3541 cgacatatgt cgctgcctgc acaactacaa tgctgtgctg gagatcacct cctccatcaa
     3601 ccgcagcgcc atcttccgtc tcaaaaagac atggctcaaa gtttctaagc agacgaaatc
     3661 tttgcttgac aagctccaaa agcttgtgtc atcagatggc agatttaaga acctcagaga
     3721 gtctttgaga aattgtgatc caccctgtgt cccttacctg gggatgtatc tcaccgactt
     3781 ggtgttcatc gaggagggga cacccaatta cacagaggac ggcctggtca acttctccaa
     3841 gatgagaatg atctcccata ttatccgaga gattcgccag tttcagcaga ctacctacaa
     3901 aatcgacccc cagccaaagg taattcagta cttactggat gaatctttca tgttggacga
     3961 agaaagcctg tatgagtctt cgctcctaat cgaaccaaaa ctccccacat gaggctgttc
     4021 tccccaccca ctggcaccgc agcgtaacgc cagctgcccc ccagctgctt ctgtgaacac
     4081 ctgctttacg attattgtat ataatcgtcg cgtgcctctg tacttgttct ccagcgtgtg
     4141 cttccccgag aatatattat ctcttcttgt cccgtaaaac cgaaatacaa atgtctgcac
     4201 tcaaaatata gatgtctgca ctctcagaaa aaaaaaaaaa aaa
//
