LOCUS       NM_027526               3236 bp    mRNA    linear   ROD 22-JUL-2024
DEFINITION  Mus musculus RasGEF domain family, member 1A (Rasgef1a), transcript
            variant 1, mRNA.
ACCESSION   NM_027526 XM_001479962 XM_986257
VERSION     NM_027526.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY120855.1, BY256830.1, AK018120.1 and AK158756.1.
            
            On or before Jun 25, 2009 this sequence version replaced
            XM_001479962.1, XM_986257.1.
            
            Transcript Variant: This variant (1) uses an alternate in-frame
            splice junction compared to variant 3. The resulting isoform (1)
            has the same N- and C-termini but is shorter compared to isoform 3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.179937.1, BC138284.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               BY120855.1         6-317
            313-521             BY256830.1         261-469
            522-1228            AK018120.1         353-1059
            1229-3236           AK158756.1         1255-3262
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 55.85 cM"
     gene            1..3236
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /note="RasGEF domain family, member 1A"
                     /db_xref="GeneID:70727"
                     /db_xref="MGI:MGI:1917977"
     exon            1..78
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1530
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="ras-GEF domain-containing family member 1A
                     isoform 1"
                     /protein_id="NP_081802.1"
                     /db_xref="CCDS:CCDS51883.1"
                     /db_xref="GeneID:70727"
                     /db_xref="MGI:MGI:1917977"
                     /translation="MFLEPQEAMPQTSVVFSSILGPNCNRQVQPGMGERGGGASSGSK
                     DLIFQDGRLTSGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFMPPHDLLARVGQIC
                     LEQRQQLEAGPEKAKLKSFSTKIVQLLKEWTEAFPYDFQDEKVMAELKAIAHRVTQCD
                     EENGTVKKAIAQMIQSLLLSLAARSQLQELREKLRSPVVDKGPVLKAKPPAAQKDILG
                     VCSDPLVLAQQLTHIELERVNSIRPEDLMQIISHMDSLDNHRCRGDMTKTFSLEAYDN
                     WFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVA
                     RLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNL
                     FVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYVLT
                     APIYSEEALFIASFESEGPENHMEKDSWKALRTTLLNRA"
     exon            79..282
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            283..405
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            406..543
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            544..765
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            766..840
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            841..933
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1036
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1116
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1308
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1432
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1505
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..3236
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3214..3219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3236
                     /gene="Rasgef1a"
                     /gene_synonym="6330404M18Rik"
                     /note="major polyA site"
ORIGIN      
        1 ccggagggta gtcgggcttc tacaccgcct gcgggtccgg tagcgggagc tgtagccagg
       61 atgtttctgg agccccagga agctatgccc cagacatctg tggtcttctc cagcatcctt
      121 gggcccaact gtaacagaca ggtgcagcct ggcatggggg agagaggagg tggggccagc
      181 agcggctcca aggacctcat ttttcaagat ggacgcctca cttctgggtc cttggaggcc
      241 ttgatggagc atctggttcc aacggtggac tattatcctg atcggacata catcttcacg
      301 tttctcctga gttcccgggt cttcatgccc cctcatgacc ttctggctcg agtagggcag
      361 atctgcctgg agcagaggca acagctagag gctgggcctg agaaggccaa gctgaagtcc
      421 ttctcgacca agattgtgca gctgttgaag gagtggacgg aggccttccc ttatgacttc
      481 caggacgaga aggtcatggc ggagctgaag gccatcgcac accgcgtcac tcagtgtgat
      541 gaggagaatg gcacagtgaa gaaagccatt gcccaaatga tacaaagctt gctgctgtcc
      601 ctggctgccc gaagtcagct ccaagagctt cgggagaagc tccggtcacc cgttgtggac
      661 aaagggcctg tccttaaggc caagccacca gctgcccaga aagacatcct gggtgtgtgc
      721 agcgaccccc tggtgctggc ccagcagctg actcacattg aactggaacg ggtcaacagc
      781 atccgccctg aggacctgat gcagatcatc agccacatgg actctctgga caatcacagg
      841 tgccgtggag acatgactaa gaccttcagt ttagaagcgt atgacaactg gttcaactgc
      901 ttgagcatgc tcgtggctac agaggtgtgc cgggtggtaa agaagaaaca ccggacccgc
      961 atgctggagt tcttcattga tgtggcccga gagtgtttca acatcgggaa cttcaactct
     1021 atgatggcca tcatatctgg catgaacctc agcccagtgg cacggctgaa gaaaacgtgg
     1081 tccaaagtca agacagccaa gtttgatgtc ttggagcatc acatggaccc ctccagcaac
     1141 ttctgcaact accggacagc cctgcagggg gcgacacaga gatcccagac agccaacagc
     1201 agccgtgaga agattgtcat ccccgtgttc aacctcttcg ttaaggacat ctactttcta
     1261 cacaaaatcc acaccaacca cctacccaat gggcacatta acttcaaaaa attttgggaa
     1321 atttccagac agatccacga gttcatgaca tggacacagg tagaatgtcc ctttgagaag
     1381 gacaagaaga ttcagagtta cgtgctcacg gcgcccatct acagcgagga agctctcttc
     1441 atcgcctcct ttgaaagtga aggtcctgaa aatcacatgg agaaggacag ctggaaggca
     1501 ctcaggacca ctctccttaa cagagcctga ggacacggcc tgcagatgcc agatgaagca
     1561 gaaaacacaa atcttggtga cattgcgaca gatgcaggat aagagcagcg aggcctcact
     1621 ggctggggtc cattttgtat ataactttta tgaaaaagaa gatggtaatt atctcacgca
     1681 tcagcctttg gcgcttacac agtccttttt tttttgttgt tgttatttat tttaaacggg
     1741 gcagggccct gttttgggga ggggcgggaa agagtatcat gggagatggt aaccatggta
     1801 acatcttatt ctaatgaaat gtagattttt attttctact tttgatgatt aacatcctat
     1861 aaaaaaaatt tttaaagaac ccaaccaaaa ccaatgtgag ccctgcctgc tcggacgcct
     1921 ctccagccag tgtctctgat gttggggtta gtgccttaga gggtactggg agtccagcct
     1981 tcctgttcca ttgttggaac tgaagccaac tctgtcctgt gccacaagga gcccaccctc
     2041 agcagcctgg gccaccaggt tcccacagga agggctggag tggccaggaa gtctcgtgtg
     2101 cacacttctg gaagggaacc tttacatacg ggctttccaa agtttacatt ttcattttcc
     2161 tttatcaggg atttatgggg ttgggagggg acaaggggat gcctgacagc atattttctg
     2221 tgagaatatg tttggcaagt cgttcttcaa ttgtgtttta gaaggaaaaa aaaagaaaag
     2281 aaaagaaaaa ccaacaacaa caacccaact ctgccaatct agggagggta ttgtagtgtt
     2341 gtcagtcttc cgggcgcaga ggcactgcag ggaagaaaac acaaaacaga ctcaaggatg
     2401 gtaaggctgt ctgaagctgg tacacaaaga gaaggaaaag aaacatgggt atgtggcaga
     2461 cagtctcccc tccctctggt aggagccccc cccctgcccc atgggtaatc tgaaactcaa
     2521 ttagccaggc agcccccacc accaaagctc ttccggatca aactgtctaa cacggagcag
     2581 tgtatttctc atccaaacag gggattgcgg ttcatctttc ctgtctgact tccctgcgtg
     2641 aaccctgggt cctgggtggg atggctctgt ccctcagaca gctggagaca tctacactga
     2701 tttgcttggc ttgggtggga gttgcctgtc tagaggccca gtatggaatg gttggtccca
     2761 ttcccagagc ttctttggac ggcctagaga ttatcggcca ggccataggg aggtgggcga
     2821 tggccaccct tagcctcggg gagccgagta gctacactgt attgtcagtg tgtttgcact
     2881 tttcagacct tctagctggt acagattgta tattgatagt acatatcgat ttgtttatgt
     2941 ctttgagata aaggctggaa acttgagaat atatatattt ggaatataat gttagagcat
     3001 ttttctgtac atgtgttaag agccttcagt ccatgtgtgc ttcaggggtc atcggttcca
     3061 gtatcttctt gagaaagtgt ctacattgct cttctattgc ccccaatttg atacaccctc
     3121 catttgatgt tttggtattt ggagttgatc tctgatctaa agctgtacca tacgcaggat
     3181 gtcagttgtg gtgtgactga atgtacttgc atcaataaaa gaacatgttg ctcttc
//
