LOCUS       NM_001404961            5364 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus RAS and EF hand domain containing (Rasef), transcript
            variant 2, mRNA.
ACCESSION   NM_001404961 XM_006537926
VERSION     NM_001404961.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Sato,K., Kadowaki,T., Takenaka,M., Konishi,M., Ando,M., Onodera,T.
            and Tsukuba,T.
  TITLE     RASEF/Rab45 regulates the formation and sorting of zymogen granules
            and secretion of digestive enzymes by pancreatic acinar cells
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (7), 167310 (2024)
   PUBMED   38901651
  REMARK    GeneRIF: RASEF/Rab45 regulates the formation and sorting of zymogen
            granules and secretion of digestive enzymes by pancreatic acinar
            cells.
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Oyama,Y., Miyata,H., Shimada,K., Fujihara,Y., Tokuhiro,K.,
            Garcia,T.X., Matzuk,M.M. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched
            genes dispensable for male fertility in mice
  JOURNAL   Asian J Androl 24 (3), 266-272 (2022)
   PUBMED   34290169
REFERENCE   3  (bases 1 to 5364)
  AUTHORS   Kumar,S., Lee,H.J., Park,H.S. and Lee,K.
  TITLE     Testis-Specific GTPase (TSG): An oligomeric protein
  JOURNAL   BMC Genomics 17 (1), 792 (2016)
   PUBMED   27724860
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5364)
  AUTHORS   Oshita,H., Nishino,R., Takano,A., Fujitomo,T., Aragaki,M., Kato,T.,
            Akiyama,H., Tsuchiya,E., Kohno,N., Nakamura,Y. and Daigo,Y.
  TITLE     RASEF is a novel diagnostic biomarker and a therapeutic target for
            lung cancer
  JOURNAL   Mol Cancer Res 11 (8), 937-951 (2013)
   PUBMED   23686708
  REMARK    GeneRIF: Results show elevated RASEF promoted cell growth via
            enhanced ERK signaling and is associated with poor prognosis of
            non-small cell lung carcinoma (NSCLC)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX294159.21.
            
            On Apr 15, 2022 this sequence version replaced XM_006537926.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.4574.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-492               BX294159.21        193562-194053       c
            493-639             BX294159.21        174194-174340       c
            640-730             BX294159.21        172703-172793       c
            731-826             BX294159.21        165775-165870       c
            827-904             BX294159.21        162602-162679       c
            905-1020            BX294159.21        152751-152866       c
            1021-1089           BX294159.21        151470-151538       c
            1090-1174           BX294159.21        147933-148017       c
            1175-1263           BX294159.21        146967-147055       c
            1264-1495           BX294159.21        143838-144069       c
            1496-1633           BX294159.21        143394-143531       c
            1634-1778           BX294159.21        143156-143300       c
            1779-1860           BX294159.21        138628-138709       c
            1861-1975           BX294159.21        137347-137461       c
            1976-2095           BX294159.21        134332-134451       c
            2096-2172           BX294159.21        130477-130553       c
            2173-5364           BX294159.21        117490-120681       c
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 36.06 cM"
     gene            1..5364
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="RAS and EF hand domain containing"
                     /db_xref="GeneID:242505"
                     /db_xref="MGI:MGI:2448565"
     exon            1..492
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..2278
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="isoform 2 is encoded by transcript variant 2; RAS
                     and EF-hand domain-containing protein homolog;
                     testis-specific GTPase"
                     /codon_start=1
                     /product="ras and EF-hand domain-containing protein
                     homolog isoform 2"
                     /protein_id="NP_001391890.1"
                     /db_xref="GeneID:242505"
                     /db_xref="MGI:MGI:2448565"
                     /translation="MEADADPEELTRLRSVFATYDTNGSGRLERAEFSALCAELHVAE
                     AEAEAVFRGLDTDRDGAITFPEFARGFRGALGRDSAEAQGAWEVAEPESPSPAWRDFH
                     ARLGDEAQLIPRKEQVSTLYQNITLVEPRLLQPYERVIRNFLREIKLQSTEMENLAIA
                     VKRAQDKAAIQLSELEEEMDQRIQAVENESRKDEKRKAEEALTDLRRQYETEVGDLQV
                     TIKRLKKLEEQSRQISQKQDVTALKKQIHDLTMENQKLKKELLEAQTNVAFLQSELDA
                     LKSDYADQSLNSERDLEIIREYTEDRSSLERQIEILQTANRKLHDSNDGLRSALENTY
                     SKLNRSLRINNISPGNTISRSSPKFNHHSSQPLAYDRSFHSSYADEDCDSLALCDPLQ
                     KMNYEVDSLPESCFDSGLSTLRDNECDSEVDYKHQGEFQTLHRTEESLGGDASDTDVP
                     DIRDEEAFDSESVASVLHWQPQGSAGEGSTLSSSRKPISALSLQTDMVDNTSKVTSQK
                     AYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTA
                     GQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVDMVEDGTHRTIPIMLVGNKA
                     DLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRTED
                     DDSRSITSLAGSTSKKSLQMKNCCNG"
     exon            493..639
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            640..730
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            731..826
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            827..904
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1020
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1089
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1174
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1263
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1495
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1633
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1778
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1860
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..1975
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2095
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2172
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..5364
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2526..2531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="hexamer: AATAAA"
     polyA_site      2551
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="major polyA site"
     regulatory      5342..5347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="hexamer: AATAAA"
     polyA_site      5364
                     /gene="Rasef"
                     /gene_synonym="RAB45"
                     /note="major polyA site"
ORIGIN      
        1 agtcgggcgg ctgcagctgg aggcgagcgt ctggctctcc tgcgccccgc ctcacccgga
       61 catccccgcc cgcggcaggt ggcggaaccg gccggacctg ctccactggc cgagccccgg
      121 agctcggagc cccgcggtgg ccagcggcgc catggaggcg gacgcggacc ctgaggagct
      181 gacccggctg cgctccgtct tcgccaccta cgacacgaac ggctccgggc gcctggagcg
      241 tgcggagttc agcgcgttgt gcgcggagct gcacgtggcc gaggccgaag cagaggccgt
      301 gttcaggggg ctcgacacgg accgcgatgg cgccatcacc ttcccggagt tcgcgcgtgg
      361 cttccgcgga gcgctcggtc gggacagtgc ggaggcccag ggcgcctggg aagtggcaga
      421 gccggagagc ccgagccccg cgtggcggga cttccatgcg cgcctcggag acgaggccca
      481 actcattccc aggaaagaac aagttagtac cctgtaccag aacatcaccc ttgtggagcc
      541 aaggctactc caaccgtatg aacgtgtgat aaggaacttt ctccgtgaga tcaaactcca
      601 gagcacagag atggaaaacc tggccattgc agtgaagaga gcccaggaca aggcggccat
      661 tcagctaagt gagctggaag aggaaatgga tcagaggatt caagctgtgg aaaatgagtc
      721 ccggaaagat gaaaagcgca aagctgagga ggctctcact gacctcaggc gtcagtatga
      781 aacagaagta ggagacttac aagtgaccat taaaaggtta aaaaagcttg aagagcaatc
      841 aagacaaata agtcaaaagc aagatgtgac ggcattgaag aagcaaatcc atgatttaac
      901 aatggaaaat caaaaactca agaaagaact tttggaagca cagacaaacg tagccttcct
      961 tcagagcgaa ctagatgctt tgaaaagtga ttatgctgac cagagtctga attctgaacg
     1021 ggatctggaa ataatccgag aatacacaga agatcgaagc agtcttgaga ggcaaattga
     1081 aatcctccaa acagctaacc ggaagctgca tgacagtaat gatggcctca ggagtgcact
     1141 ggaaaacact tacagcaagc tcaacagatc cttgcgtata aataacatat ctccagggaa
     1201 tacaatttct agaagcagtc ccaaatttaa tcatcattcc tctcagccac tggcctatga
     1261 caggtcattc cattcttcat atgcagatga ggattgtgac tctttggctc tctgtgaccc
     1321 tctgcagaag atgaattatg aagttgacag cctgcctgaa agctgttttg acagcggtct
     1381 gtctactctg agagacaatg agtgtgactc cgaggtggac tacaagcatc agggagaatt
     1441 tcagactttg cacagaacag aggagagcct tgggggcgat gcatcagaca cagatgttcc
     1501 agatataagg gatgaagaag cgtttgattc tgaaagtgtg gcctctgtct tacactggca
     1561 gccccagggc tctgctggtg agggcagcac tcttagttcc tccagaaagc ccatctcagc
     1621 tctttcactt cagacagaca tggtggataa cacctccaag gtcacatctc agaaggctta
     1681 caagattgtg ctggctggcg acgccgcagt gggaaagtcc agcttcctca tgcgtctctg
     1741 caagaatgag tttcagggga acacaagcgc aactctagga gttgatttcc aaatgaaaac
     1801 tctgattgtg gatggtgaac aaacagttct tcagctctgg gatacagctg ggcaagagag
     1861 atttaggagt atcgccaagt cttacttccg aaaagcagat ggtgtcttac tgctatacga
     1921 tgttacttgt gagaaaagct ttcttaacgt ccgagaatgg gtggatatgg ttgaggatgg
     1981 aacccacaga actattccta tcatgttagt aggaaacaag gctgatcttc gtgatgttga
     2041 taatgcagag aatcaaaagt gtatatcagc atatcttgga gaaaaactgg ccatgaccta
     2101 tggggcatta ttctgtgaaa caagtgccaa agatggctcc aacgtggtgg aagctgttct
     2161 ccatcttgca cgggaagtga agaaaaggac agaggatgac gacagcagat ctatcaccag
     2221 tctggctggg tctacttcta aaaagtcact gcagatgaag aactgttgca atggctaaag
     2281 cccagttgtt ctgtgttcac aaagtccttg cttccagatt actgactgtg ggaccctggc
     2341 tctccatgaa gcagtgcata atatgacact gggatctgag gaatcacagc ctggccaatt
     2401 gcactgttct tccccgctct cagcaacctg aagcttgctt tgttctatct cagtgagcta
     2461 tttgggatgg ctggccctat agatctaaga gaaaatgtgt taatgttttt aatatgatat
     2521 ggtaaaataa aataacattt ctcagatcaa agctattgag aaaccactta taacactgct
     2581 ttataagaaa acctgtagtt tgtaattatc ctgtgacagt gtttacttaa aattttggac
     2641 aaaagagtat aatactttat ttgtattatg gaactcagtg gtttaaaaca gagaattagg
     2701 caactggcag tgtattatat attacagttt taaaccatgg ctggtgtaag gtagcatgaa
     2761 tggtttcatt tggcacctca gcttagcctg ataacatggt gcctgttcag gccacattac
     2821 aatgttccca tctggaaaac aagtaagttg ttctctaggg cctttcagat tctgtagcat
     2881 cttaagaagg gggaagggtg cagtggtcca cagtcaggaa ggaccactgt gaagtctgtg
     2941 gcttaaatca ttaagtgaaa atgttagtaa agatgtgtat aatacatact actgggagaa
     3001 ctatgccacc aacaggtatc tcacatagtg actgccagag gctgggggaa gaagggagtt
     3061 ggcactaatg gtcaaccttc ttaggaggat ggccacagtc tcatggcatc tgcctggcag
     3121 tagtcgtgct aaaagccacc aaactcagta tttgaaaagg ataagtgttg agaatgtgaa
     3181 ttatgtttca tggaaaatat aatggtaact gaagtaacaa tactttgcca aatgaatata
     3241 ttgtcgcgat ttcacattgt cctgtggttt aacatattat ctgtagcata atgccagccc
     3301 ctcagcacct aacttcaagc attaactttc tccttgatga acaatgtaag gttgttatta
     3361 agcatagctt tggaatgtgc tgcatctaaa agaggaccag tctttaaaac tttgagtctg
     3421 tcctttagat agtgactaaa ggacactatc cttactagtg tgactgctcc tcactggttt
     3481 tgtttttctc ctggaacctg cagtgccttc tgaccaaaat gtcttccaag agtcagaagc
     3541 ttcactatct gactcaggat gggccgacaa aaacatgttt ctagtggcct gaaccaatta
     3601 gattattctt agtaagagac tcagatacct tggttcatat agatctaggc tttgaatagc
     3661 ttacccagaa cataaataat cgctgttact tatgtaagtt gtatagtatc tgttcaagca
     3721 ttcttttttt ttagctaaaa tagttgctta ggctagtgag gtgtctcagg aagtacaagt
     3781 acttgttgca caagcttgcc aactccagat ttgtacctga ccccacagtg gaagggcaga
     3841 actgtctcct gaaagtggtt ctctaacctt catatgtgtg ttctagtgaa caaatgatat
     3901 gtccacacac atagaatagt agatattttt taataacact tcctttaaac aaacccttct
     3961 gtgcttttga tgtttgtcat taaactttaa attgtttgag cttcattata tttttttaat
     4021 aaatgggaac tgactagata catttataca caaaagttac ataccttatc cctatagaaa
     4081 tactctaggg tcttaacaag aaaattgaat cttcagtatc tacataggaa ttgctatttt
     4141 gtggttaatg gtttctagct tcagctgaaa ggttactgat taaaaagaat acattgattg
     4201 tgaggaatgg ccagagaaat tggcacacag gttgaggtgg tggtcatttt gtaatatatg
     4261 tcacagttag aagtttgcta ttagtctcac cttcaggtgt gggtttccaa gacaaggctg
     4321 agtcttatac attgatggat tgcactcacc gaacagactc tgttagcatg tgtgctagcc
     4381 tgttctcttt atttattttc cccaaagtcc ttttgtcttc tattctctgt aaccaagggt
     4441 gtattattaa tgcagacgtt ctgcctgggg ttttgcctct agttgagggg ccagaacagc
     4501 tgtgtttacc agaaagttgt ctaggtgaca tgactgacaa gcttagtgat ctgaatataa
     4561 aatattcaaa ttaaagatta ctatctctac ataacaaact tgtttaagga aaagctaaat
     4621 caaactattt ttatctgatt ttttgggaga ctgttttgat ttcttgaaaa gtgttctcaa
     4681 atttaaatta tattttaagt aaagacaaaa taatgttttc agcttaagct tacttagata
     4741 tattaacaca tttctcacaa ataatgtatt ttgtattcat ttttccttag acaactttta
     4801 aaaacccaac gatgctatat cttcctttta aatactctct aacacattgg ataatgtatt
     4861 aattatcaaa aataatgttg gagaagcagg catttttttc actgttaact tctaggcctg
     4921 ttaattttta taatttattt ttcttcttgc tgctttatac aatgttatat aacatttaac
     4981 tatttaatac tgtgtgattt gaataaataa aactgtcctt cctcctctaa tgttaaaatt
     5041 gaggttaagg gtaaaggctt attttcatat ttatacagtg gttagactca gaatattgtt
     5101 ttgtaggttt ttgttgtata tatgtataag aaattcagtg tgtatatgac aggtaggcaa
     5161 ctgggaactg agttactctt atgtatatga ttttggttta tatggtttgc atttttcaca
     5221 ctttaagctg tatttcatcc tactgtttat atcttttcaa acaattgaaa taccataaaa
     5281 tataattgta taacaatgaa acttttccaa ggagctacag aatctgtgcc ttaaaaaata
     5341 caataaagtg ctcttctttt ggcc
//
