LOCUS       NM_177644               9979 bp    mRNA    linear   ROD 15-JUL-2024
DEFINITION  Mus musculus RAS protein activator like 2 (Rasal2), transcript
            variant 1, mRNA.
ACCESSION   NM_177644
VERSION     NM_177644.6
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Zhang,Y., Yang,Y., Hu,X., Wei,B., Shen,Q., Shi,C. and Chen,P.
  TITLE     RAS protein activator-like 2 (RASAL2) initiates peritubular
            capillary rarefaction in hypoxic renal interstitial fibrosis
  JOURNAL   Transl Res 269, 14-30 (2024)
   PUBMED   38453052
  REMARK    GeneRIF: RAS protein activator-like 2 (RASAL2) initiates
            peritubular capillary rarefaction in hypoxic renal interstitial
            fibrosis.
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 9979)
  AUTHORS   Hu,S., Zhao,Y., Qiu,C. and Li,Y.
  TITLE     RAS protein activator like 2 promotes the proliferation and
            migration of pulmonary artery smooth muscle cell through
            AKT/mammalian target of Rapamycin complex 1 pathway in pulmonary
            hypertension
  JOURNAL   Bioengineered 13 (2), 3516-3526 (2022)
   PUBMED   35044284
  REMARK    GeneRIF: RAS protein activator like 2 promotes the proliferation
            and migration of pulmonary artery smooth muscle cell through
            AKT/mammalian target of Rapamycin complex 1 pathway in pulmonary
            hypertension.
REFERENCE   4  (bases 1 to 9979)
  AUTHORS   Wang,B., Li,X., Hu,W., Zhou,Y. and Din,Y.
  TITLE     Silencing of lncRNA SNHG20 delays the progression of nonalcoholic
            fatty liver disease to hepatocellular carcinoma via regulating
            liver Kupffer cells polarization
  JOURNAL   IUBMB Life 71 (12), 1952-1961 (2019)
   PUBMED   31408278
REFERENCE   5  (bases 1 to 9979)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9979)
  AUTHORS   Zhu,X., Xie,S., Xu,T., Wu,X. and Han,M.
  TITLE     Rasal2 deficiency reduces adipogenesis and occurrence of
            obesity-related disorders
  JOURNAL   Mol Metab 6 (6), 494-502 (2017)
   PUBMED   28580280
  REMARK    GeneRIF: Rasal2 deficiency leads to impaired adipogenesis in vivo
            and in vitro.  Rasal2 deficiency resulted in increased activity of
            Ras and ERK in preadipocytes.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9979)
  AUTHORS   Li,N. and Li,S.
  TITLE     RASAL2 promotes lung cancer metastasis through
            epithelial-mesenchymal transition
  JOURNAL   Biochem Biophys Res Commun 455 (3-4), 358-362 (2014)
   PUBMED   25446096
  REMARK    GeneRIF: the down-regulation of RASAL2 promotes metastatic
            progression of lung adenocarcinoma
REFERENCE   8  (bases 1 to 9979)
  AUTHORS   McLaughlin,S.K., Olsen,S.N., Dake,B., De Raedt,T., Lim,E.,
            Bronson,R.T., Beroukhim,R., Polyak,K., Brown,M., Kuperwasser,C. and
            Cichowski,K.
  TITLE     The RasGAP gene, RASAL2, is a tumor and metastasis suppressor
  JOURNAL   Cancer Cell 24 (3), 365-378 (2013)
   PUBMED   24029233
  REMARK    GeneRIF: The RasGAP gene, RASAL2, is a tumor and metastasis
            suppressor
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119897.8, AC138382.10 and AC119220.11.
            
            On Feb 4, 2022 this sequence version replaced NM_177644.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC120902.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-798               AC119897.8         141151-141948       c
            799-926             AC138382.10        135047-135174       c
            927-1050            AC138382.10        122641-122764       c
            1051-1157           AC138382.10        65949-66055         c
            1158-1267           AC119220.11        7316-7425
            1268-1421           AC119220.11        20920-21073
            1422-1520           AC119220.11        22428-22526
            1521-2075           AC119220.11        23955-24509
            2076-2220           AC119220.11        25885-26029
            2221-2365           AC119220.11        35421-35565
            2366-2602           AC119220.11        36233-36469
            2603-2804           AC119220.11        38846-39047
            2805-3004           AC119220.11        41314-41513
            3005-3866           AC119220.11        42405-43266
            3867-4001           AC119220.11        50265-50399
            4002-4204           AC119220.11        52340-52542
            4205-4292           AC119220.11        53895-53982
            4293-9979           AC119220.11        59297-64983
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 67.97 cM"
     gene            1..9979
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /note="RAS protein activator like 2"
                     /db_xref="GeneID:226525"
                     /db_xref="MGI:MGI:2443881"
     exon            1..798
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             597..4457
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="ras GTPase-activating protein nGAP isoform 1"
                     /protein_id="NP_808312.3"
                     /db_xref="CCDS:CCDS48408.1"
                     /db_xref="GeneID:226525"
                     /db_xref="MGI:MGI:2443881"
                     /translation="MELSPSSGGAAEALSWPEMFTGLESDSPLPPEDLDAVVPVSGAV
                     AGGMLDRILLESVCQQQSWVRVFDVKGPPTHRLSCGQSPYTETTSWERKYCILTDSQL
                     VLLNKEKEMPVEGQDQQTDSTKGRCLRRTVSVPSEGQFPEYPQEGTTKLEVPAERSPR
                     RRSISGTSTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSL
                     RNADDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEV
                     TYLSGSKCFSCSSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKY
                     FCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNN
                     YVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKE
                     FAEFITSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCG
                     EHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSE
                     LMDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASL
                     FLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMND
                     FLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENS
                     FLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFAEHSSSPNVSGSLSSGLQRICE
                     DPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKENSLPNGRSISL
                     MDLQDTHAAQAEHASVMLDVPMRLAGSQLSITQVASIKQLRETQSTPQSAPQVRRPLH
                     PALNQPGSLQPLSFQNPVYHLNNPVPAMPKASADSSLENLSTASSRSQSNSEDFKLSG
                     PSNSSMEDFTKRSSHSEDFSRRHTVPDRHIPLALPRQNSTGQSQIRKLDHSGLGARAK
                     APPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQT
                     QQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKER
                     LRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRL
                     MAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVR
                     NGISPTNPTKLSITENGEFKNSSC"
     exon            799..926
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1050
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1157
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1267
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1421
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1520
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..2075
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2220
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2365
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2602
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2804
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..3004
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3866
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3867..4001
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4002..4204
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4205..4292
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..9979
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9954..9959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      9979
                     /gene="Rasal2"
                     /gene_synonym="6530401P13; A330066M24Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaggagcgc gagccggagg agcccaagca gcggccggag cggcggcctg agagttagcg
       61 ggccggcctg cgcgttccgc gggagcaagc cgacgccagg cggacagaag ctgggccggg
      121 gcgcgcccct caggctgagg gagccggcgc tttgagcccc cgaggcgacg gcggcgggag
      181 ccggtcagag ggggcggccc ctggcggcga cgcccccgag cccagccctg cctggccgag
      241 ctccatcctc agcccaagcc cgggcggccc gcgaacgcgc ccgcgggggt tgagcagggc
      301 tgcgtggggg cgggaggtga gcccgcgctc ccgggtgctc cgcgtccgct ccggacccgc
      361 acctgagccg ggacccgcct ccgctccgga ccacgctgga cccgcctccc ggcccgggtc
      421 ccaccctccg cttgcaggtg ggctgcggcg cccggccttc gggcggtggg cggcggggaa
      481 ggaggtgagc ggtgtccgcg cccgcagccg ttcgggtgct ggtggccgcg ccgtcgtccc
      541 tttcccgcag ccgggaagcc cgccccgccg ccgccgcctg ctccctctgg gacaccatgg
      601 agctgtctcc gtcgtccgga ggagccgcgg aggcgctgtc gtggccggag atgttcacgg
      661 ggctggagtc cgactcgccg ctgcccccgg aggatctgga cgcggtcgtc ccagtcagcg
      721 gagcagtggc cggtggcatg ctggatcgga tccttctgga gtcagtgtgc cagcagcaga
      781 gctgggtccg ggtgttcgat gtaaaaggcc caccaaccca ccgtctgtct tgtggtcagt
      841 caccctatac tgagacgaca tcatgggaga ggaagtactg catcctcaca gacagccagt
      901 tggtgttgct caacaaggag aaggagatgc ctgtggaagg acaggatcaa cagacagatt
      961 ccaccaaagg gcgctgtctg aggagaactg tcagtgttcc ttcagagggt cagtttccgg
     1021 agtacccaca ggagggcacc actaaactag aggtgccagc agaaaggtcc ccccgcagac
     1081 ggagcatctc agggaccagc acatcagaga aacccaactc catggacact gcgaatacct
     1141 ctccttttaa agtaccagga ttcttcagca agcgcctgaa aggttccatc aagaggacca
     1201 aaagccagtc gaagcttgac agaaacacaa gctttcggct tccatccctt cgcaatgcag
     1261 atgacaggtc tcgaggtctg cctaaactga aagagtcgcg ttcccatgag tccctgctga
     1321 gcccatgcag tgcagtggag tgtctggatc taggtagagg agagccagtc tcagtgaagc
     1381 cactgcacag tagcatcctc ggacaggact tctgcttcga ggtcacctac ttaagtggaa
     1441 gtaaatgctt tagctgtagc tctgcctccg agagagataa gtggatggaa aaccttcgaa
     1501 ggaccgttca gccaaataag gacaattgta gacgagctga aaatgttctc cgtttatgga
     1561 tcattgaagc caaggacctt gcccctaaaa aaaaatattt ctgcgaactg tgccttgatg
     1621 ataccctctt tgctcgtaca accagcaaga ccaaagcaga caatattttt tggggtgaac
     1681 attttgaatt ctatagcctt ccacctcttc atagcatcac ggttcatatt tataaggatg
     1741 tggaaaaaaa gaaaaaaaag gacaagaata attatgtagg gctagtcaat atccccactg
     1801 ccagtgtgac tggtcgccag tttgtagaaa agtggtaccc agtgagtaca cccacaccca
     1861 acaaaggaaa gacaggagga ccttctattc ggattaagtc acgtttccaa actatcacca
     1921 ttctgcctat ggagcaatac aaagagtttg cagaattcat caccagcaac tacaccatgc
     1981 tgtgttctgt tcttgagcca gtaattagtg tgaggaataa agaggagttg gcttgtgcct
     2041 tagtacacat tcttcaaagt actggcagag ccaaggattt tctgactgac ttggtgatgt
     2101 cagaggtaga tcgttgtgga gaacatgatg tcttgatctt cagagaaaac accattgcca
     2161 ctaaatccat tgaggaatac ctcaagttgg tgggacaaca gtaccttcat gatgcactcg
     2221 gggagttcat caaagccttg tatgaatcag atgagaactg tgaagtggat cccagcaagt
     2281 gttcatctag tgaactgatg gaccatcaga gcaacctgaa aatgtgctgt gagttggctt
     2341 tctgcaagat catcaactca tactgtgtct tcccgcgtga gttgaaagaa gtgtttgcat
     2401 catggaagca gcagtgcctg aaccgaggca agcaagacat cagtgagcgg ctcatcagtg
     2461 cctcattgtt tctccgtttt ctttgcccag ccattatgtc tcccagtctt ttcaacctga
     2521 tgcaggaata tcctgatgac cgcacatctc ggacactaac tcttattgcc aaggtcattc
     2581 agaacctggc caactttgcc aagtttggta acaaagagga gtacatggca ttcatgaatg
     2641 attttttaga acatgaatgg ggtggaatga agcggttcct tttggagatc tctaatcctg
     2701 acaccatctc aaacacccca ggcttcgatg gttacattga tttgggcaga gagctgtcag
     2761 ttttgcattc cttactatgg gaagtagttt cccaacttga taagggtgaa aattcctttc
     2821 tacaggcaac tgtggcaaaa ctgggacctc tccctcgtgt tcttgctgac attaccaagt
     2881 ctctgactaa tcctacgcca atacagcagc agctaagacg tttcgctgag cacagctcca
     2941 gtccaaacgt cagcggaagc ctctcctctg ggctgcagag aatatgtgaa gaccccactg
     3001 acagtgattt gcataaacta aagtctccaa gccaggataa cacagacagc tatttcagag
     3061 gaaagacgtt attgttggtt cagcaagcct cctcccagag catgacgtac tctgaaaagg
     3121 atgaaaagga aaacagcctt cctaacggtc ggagcatctc cctcatggac ctccaggaca
     3181 ctcatgctgc tcaggcggag catgcatctg tcatgcttga tgtgcctatg cgccttgcag
     3241 gaagccagct ttctataacc caggtagcta gcatcaaaca gctgcgggaa acccagagca
     3301 ctccccagag tgcacctcaa gtgagaagac ccctgcaccc agccttgaac cagccaggaa
     3361 gtctccagcc cttgtcattc cagaatcctg tctatcacct caacaaccca gttccagcaa
     3421 tgccaaaggc ctctgcggat tccagtttgg agaacctaag cactgccagt tctagaagcc
     3481 aaagcaatag tgaagacttc aaactcagtg gacccagcaa tagcagcatg gaagatttca
     3541 ccaaaagaag ctcccacagt gaagacttct ccaggcgcca cactgtacca gacagacaca
     3601 tacctcttgc tctaccacga caaaatagta cggggcagtc ccaaatccga aaactggacc
     3661 attctgggtt aggtgcccga gccaaagcac ccccatctct gccacacagt gcttccttgc
     3721 gtagcacagg gagcatgtca gtggcgtctg cagccctaat ggctgaacct gtgcagaacg
     3781 ggagccggtc caggcagcag tcctcttcct ccagagagag ccctgttccc aaagtgagag
     3841 caatccagag acaacagaca caacaggttc agtcacctgt ggactccgct acaatgtccc
     3901 cagtagaaag gacagcagcc tgggttctga acaatggaca gtatgaagag gatgtggaag
     3961 aaactgaaca aaatcaagat gaagccaagc atgctgaaaa gtatgaacaa gaaattacca
     4021 agctgaagga gcgcctacga gtgtccagcc ggcggttgga ggagtatgag cgccggctgc
     4081 tggtgcagga gcagcagatg cagaagctgc tgctggagta caaggcccgg ctggaggata
     4141 gtgaggagcg gctgcgcaga cagcaggagg agaaggacag ccagatgaaa agcatcatca
     4201 gcaggctaat ggctgtggaa gaagaattaa agaaagatca tgctgagatg caagcagtta
     4261 ttgatgcaaa gcagaaaata attgatgcac aggaaaagcg gatcgtgtcc ctggattcag
     4321 ccaacacgag actgatgagt gcgctgacac aagtgaagga gcggtacagc atgcaggtcc
     4381 gcaatggcat ctctcccacc aaccccacca agctttccat cacggagaat ggtgaattca
     4441 aaaatagcag ctgctgatgg ccttggtgac acagactgtg ggaggaggca gaaggaagtt
     4501 gcccagctct cctatcccag ccctttaacc ctccaggttt acagaatgtt atttcaaatg
     4561 gcagtgtggt gagaaactcc taaatgaaga aaggaacctt gtctttcagg gcttaaagca
     4621 aagacttgaa gtttgatgct tttcccagtg tctctatgta catagggaac ttagttctgg
     4681 gccgtgtaca gaaataccac tgtaatatac caaaaggaag ttaataatgt agattacctt
     4741 tttaattatt actattttat tattgttttt ctcttattga aagcactgca gttgttagtg
     4801 gaggaaaagt aggagccaag tgtgagtgag gaataagcct attggttcag taggtagagt
     4861 tcatgtatta gataggagca catggagtac gataggacct tgtcggaatg gatttttttc
     4921 agggattcat ctgccagttt aaaagcccca ccatagtcct taccattagg aaaacttgca
     4981 aataccaaga tacatacaaa acagtaattt atgccactga tcaaccaaga tgcttctaac
     5041 agactgtaac ctgctttgga gagccgccgt ttcagaagac acagataaaa gtggttgact
     5101 gtaccaaaac tagactagga atgatttctc ttgaactcct gtcaatattt atttcctcca
     5161 tctataatgg acagttagat gtgggatttt ttgattttac tccaactttt tttaaagtgc
     5221 tctttagagc tgacagattt gtaagtaccg tgtctctatc tactgtcttc atgttcactt
     5281 gtccactgcc ctttcctccc aggctgggag cggtcagcac caacaggact cactctcccc
     5341 tggagagtgg ccattcaagt agaccactca taagccatct gactaaatac cctttttcca
     5401 cacagaaaag ggctaactat acagcagagc aggaatctct gaatatccca ctaaacgttc
     5461 tcttcctatt tgtcagttgt atttttcctt aggataaagg ttttatccac tatattagca
     5521 aatctgtggg cagggtaacc cgtcaccact taacatgaat tgcttcctct ttctatttat
     5581 cctctccata aaattttgga agctatctaa aactgagaca tgccccttaa aaatgatgat
     5641 taatcttgct agagaaagga tgacacattc tggttgtcat tccacactca tggtagactc
     5701 agcaagactg acctagatta tcattaatga ctttgatata ggacagaaaa tctgtggttt
     5761 tctccagagt gtgacatgtc atctggaaat ctggaatgca ggcttgatta ccagagatat
     5821 ggaaagttag atgcaggtta agcaaatatt ttatatatat ataaatatat atatatacat
     5881 atattcacac atgtacatac aatgtgcaca tcaaaaatgg tcatatcttc tgtgctgtca
     5941 gatatacaga gataaatgtt taaaagggtc ttgaatctct ttaagcaaga tctacacagt
     6001 tttacaaggg aaaaaaacat ttctcgaata agtcaaagtt tcattttgtt ttttaagtta
     6061 taaacttcaa gaattttttt ctaagtaaag gacgaggggg gagacataac ggagccatat
     6121 gcatggagta tgtgtttcta cataacagaa aacttacgag gaaacgagag aacatgtata
     6181 gttccctcct atttgaagcc caggtgtaga taccaaggcc tccttccccc caagtcccag
     6241 gggtgcattc caagcctcac ttgctcctca gttgaccttt gggtctgacc ttgtacttca
     6301 gccacctcac cagagccctc agcctacaac ctgtgaaagg gtttcttcat aactcttaaa
     6361 agcaggagaa tcccactatg cttagaactt caggctccac gcccacttga gccttctcct
     6421 gaccctcagc agttctttta catcttatct cttccctcca agctaaggtc acagtgtggt
     6481 taaaattact tcccaagaaa gaaggttatt gattcttcag taatgccctg tattggagcg
     6541 gggggggggg gggggggtaa acatagatct tttcatgttt gtagcctagc agtgtttcct
     6601 tcattcttca aaatccttca gtactaccac acaaaaccta gtcaaatatc agtgttggtg
     6661 gccagaagta tataatgtgg ataatgtctt gctttgaggt agggaaggtc ttcaagtgca
     6721 ggttatagat tacagagagc tggccatatt gatgaatgca gtttaactat taacattttg
     6781 ggaccttctt aaagagcctg tctgtattac aagattgaat gcccaaagca tatttcaagg
     6841 ttctgtaatg ttaccatgaa tgaaggaaaa aagtaaaaaa ataagtctta agagagttag
     6901 gggtcgaaag ttcagcattg ttgcctgata ttaaatctta gattatcctt tgagcctact
     6961 catattttta gcctatcaga aatattctga actggggtat agctcagttg gcagagtgcc
     7021 agcctagcat tccagaggcc ctaggttcaa acaccagcat ggtctgaatt gggggggtgg
     7081 cacagacctg cagtcccagc actgtaggga taggagcagg agcattggca gccccagggc
     7141 acccaccctc tgcctcggaa gtttcctggc caacctaagc tgcttgagac cctgtctcca
     7201 aactgataac agaatggttt ttgagtattt ctttagagaa tgttacaatc ctataaagtt
     7261 tattttggaa ttgttttgag tataggagag gacagcagtg ggtgttttct atgtcatttc
     7321 tttaccctaa ataagtttct ttatcctgaa caaaaatcgt tttgctttgc ttaggaggag
     7381 atgtttttat aaagctgatg gctcctagga tccctgtgtt tgtctacagc tcaacacagt
     7441 agtaaggggt gagactagag caggcttagg tgggtgtgga tttggcacac acacacacac
     7501 acacacacac acgtcacttg aagcagattt cagaagagca aaaagtcaca ttcccatgag
     7561 agtagcatta cttcagtgtc tgtcccctgt gtactgcttg ggtctgggac agtctttctc
     7621 ggaatggaaa gtggggccat tatgttacta tagctcttct taataccgtg tattcgcaca
     7681 gcctcctcct ttcctctgct atggagttga acagtaaaaa catgagtgtt tctcagacga
     7741 agtgcacatg tgtgcactgg ggctgagcat gacttcataa gcagtctata ccagtggctt
     7801 ttatatgggt tggcggctct ccagagccct ggctcccgct gtgtccatat gcacagccaa
     7861 ttgggagcag gttttatttg aagttccaga gaacagtgcc atcaagtggg cttgcattag
     7921 tgtttgctct gcttctgtcc agatttagtt ctggttcctc agaaccattc agtagactga
     7981 cagagctaca ggctggaccg tagactagcc atgtcactaa ctcaattcag caactctcca
     8041 gcccatctca cggctttctc agccgtgtta cttattctca gcaacgttgg ggactgtctt
     8101 ctgaagacag atcaagcaag ttcaaaggac attctgctct atagcacttg acttttccta
     8161 gtcagtaact tgaactttcc tgcttgcctc agcagtaaaa gtcaccttcc taccccatac
     8221 ctggcatacc tttttaaatt attattatta ttattattat tattaattta aaggactttt
     8281 ttctttcttt cttttttttt tggttggttt ggtttggttt gggggatttt ttttttggtt
     8341 ttttggtttt tttgagacag gatttctctg tgtagcccag gctgttctag aactattctg
     8401 tagaccaggt tggcctcgaa ctcaagagtt ccacctgcct ctgcctccag agtcctggga
     8461 ttaaaggtgt gtgctgccac tgcctgacca tacatgtttc ttaaagcaag taatgtttag
     8521 gcccattttc tgtttgaata attgttatca gttcttctag aacattctcc tgtcactgat
     8581 ccatctagga aagcacttga gctttgaaag gacaagggtg gtgaaatttg catgagagag
     8641 aataaatttc tttcagaaag atcctgagga agacacatga tccaatcgct tgttgctcag
     8701 aagttcaagg aaacaagtgg agtggcgtcc cctggctctt tctaggatga gtgtgtgtgt
     8761 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gaaggcagct cctgagacaa
     8821 catgtaacat gcctacagaa ataccactct taggacactc ctttaaaaaa aatgttaatc
     8881 tactttgtaa accagtaact cccagacttt cttgaggctt tgtttttgtt tggttggttt
     8941 ttagatttta aaacattcta ttcactttga aatgtactat atgtgttata tgtatatata
     9001 agttatagtg acttgaactc tgtttggcat aggctgagaa ctattgttgg tagcatagag
     9061 tgtacagagc agccgctgac tgtcaccatc tgagagcagg atacagaagg gagtttggtt
     9121 cctcagcagt gaatgcccag gtgcttgagc aatggcccag ccagcctcag tgaggacggc
     9181 aagccaccca aggagaggag aagagaaact gacccgctgt cccagcacac aggtttaatt
     9241 ttgtactctt tcatttcttt atcaaatgac ctcattattc cctaaaaagt cttggaactg
     9301 aaaaccagac tgaactgtcg gtgcagtggc atttccacag ctgtgcttcg ggggttctgt
     9361 cctctgtgtg tggtgcaagt atttagaatt aatagcatgg atatgtcatg tccaaaccct
     9421 caagggaaaa ttaccaattt ccacttaacc aacagatgaa atgctagtta actcaatgcc
     9481 cactatcctg cagaaaagct acagtcagta gtaaagggtg gggttggctg gtgctgatac
     9541 tccttagtcg tatagaccaa ctcaagcaac agcagcatta tttactgggt cacacctggg
     9601 aggaacttag cttccaaatt tggtttttgc ttttcatctc cagggcctta ataagagtgt
     9661 gttaatacac atgactattt tacagataat tcaaaataat ttatttttta tttgaagaaa
     9721 gcgaaatcca ccttaaaaaa aaacaaaacc caagatttta ttttattttt aaagccatat
     9781 ttttgtgctt tgtagcactt agattgttcc atatgtgatc catatctgca tttcattacc
     9841 tgggttctaa aaaagattta tttttgttct gtgaataatt tttgatggtt cttgtacatg
     9901 tacagatata gatacgtttg aggtctttta ttgatattcc tacaaaaaat acaaataaac
     9961 tttaacttta aacatttca
//
