LOCUS       NM_001359144            3150 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus RAS protein activator like 1 (GAP1 like) (Rasal1),
            transcript variant 3, mRNA.
ACCESSION   NM_001359144
VERSION     NM_001359144.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Shen,X., Tan,J., Liu,R., Zhu,G., Rooper,L. and Xing,M.
  TITLE     The genetic duet of concurrent RASAL1 and PTEN alterations promotes
            cancer aggressiveness by cooperatively activating the PI3K-AKT
            pathway
  JOURNAL   Mol Oncol 19 (1), 248-259 (2025)
   PUBMED   39032134
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3150)
  AUTHORS   Xu,R., Hoss,C., Swiercz,J.M., Brandt,D.T., Lutz,V., Petersen,N.,
            Li,R., Zhao,D., Oleksy,A., Creigh-Pulatmen,T., Trokter,M.,
            Fedorova,M., Atzberger,A., Strandby,R.B., Olsen,A.A., Achiam,M.P.,
            Matthews,D., Huber,M., Grone,H.J., Offermanns,S. and Worzfeld,T.
  TITLE     A semaphorin-plexin-Rasal1 signaling pathway inhibits gastrin
            expression and protects against peptic ulcers
  JOURNAL   Sci Transl Med 14 (654), eabf1922 (2022)
   PUBMED   35857828
REFERENCE   4  (bases 1 to 3150)
  AUTHORS   Thaker,Y.R., Raab,M., Strebhardt,K. and Rudd,C.E.
  TITLE     GTPase-activating protein Rasal1 associates with ZAP-70 of the TCR
            and negatively regulates T-cell tumor immunity
  JOURNAL   Nat Commun 10 (1), 4804 (2019)
   PUBMED   31641113
  REMARK    GeneRIF: ZAP-70-associated Rasal1 as a new negative regulator of
            T-cell activation and tumor immunity.Rasal1 binds to the kinase
            domain of ZAP-70.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3150)
  AUTHORS   Xu,X., Tan,X., Tampe,B., Wilhelmi,T., Hulshoff,M.S., Saito,S.,
            Moser,T., Kalluri,R., Hasenfuss,G., Zeisberg,E.M. and Zeisberg,M.
  TITLE     High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation
            rescues gene expression and attenuates renal fibrosis
  JOURNAL   Nat Commun 9 (1), 3509 (2018)
   PUBMED   30158531
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3150)
  AUTHORS   Bechtel,W., McGoohan,S., Zeisberg,E.M., Muller,G.A., Kalbacher,H.,
            Salant,D.J., Muller,C.A., Kalluri,R. and Zeisberg,M.
  TITLE     Methylation determines fibroblast activation and fibrogenesis in
            the kidney
  JOURNAL   Nat Med 16 (5), 544-550 (2010)
   PUBMED   20418885
  REMARK    GeneRIF: We demonstrate here that hypermethylation of RASAL1,
            encoding an inhibitor of the Ras oncoprotein, is associated with
            the perpetuation of fibroblast activation and fibrogenesis in the
            kidney
REFERENCE   7  (bases 1 to 3150)
  AUTHORS   Kurrasch,D.M., Cheung,C.C., Lee,F.Y., Tran,P.V., Hata,K. and
            Ingraham,H.A.
  TITLE     The neonatal ventromedial hypothalamus transcriptome reveals novel
            markers with spatially distinct patterning
  JOURNAL   J Neurosci 27 (50), 13624-13634 (2007)
   PUBMED   18077674
REFERENCE   8  (bases 1 to 3150)
  AUTHORS   Perelygin,A.A., Scherbik,S.V., Zhulin,I.B., Stockman,B.M., Li,Y.
            and Brinton,M.A.
  TITLE     Positional cloning of the murine flavivirus resistance gene
  JOURNAL   Proc Natl Acad Sci U S A 99 (14), 9322-9327 (2002)
   PUBMED   12080145
REFERENCE   9  (bases 1 to 3150)
  AUTHORS   Minagawa,T., Fukuda,M. and Mikoshiba,K.
  TITLE     Distinct phosphoinositide binding specificity of the GAP1 family
            proteins: characterization of the pleckstrin homology domains of
            MRASAL and KIAA0538
  JOURNAL   Biochem Biophys Res Commun 288 (1), 87-90 (2001)
   PUBMED   11594756
REFERENCE   10 (bases 1 to 3150)
  AUTHORS   Allen,M., Chu,S., Brill,S., Stotler,C. and Buckler,A.
  TITLE     Restricted tissue expression pattern of a novel human
            rasGAP-related gene and its murine ortholog
  JOURNAL   Gene 218 (1-2), 17-25 (1998)
   PUBMED   9751798
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125183.4.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1 and 3 both encode the same isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.4950.1,
                                           SRR7974084.24817.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC125183.4         62356-62454         c
            100-246             AC125183.4         61732-61878         c
            247-303             AC125183.4         58396-58452         c
            304-417             AC125183.4         56347-56460         c
            418-479             AC125183.4         56137-56198         c
            480-609             AC125183.4         51634-51763         c
            610-691             AC125183.4         49174-49255         c
            692-823             AC125183.4         48951-49082         c
            824-909             AC125183.4         48262-48347         c
            910-1027            AC125183.4         47423-47540         c
            1028-1181           AC125183.4         47142-47295         c
            1182-1246           AC125183.4         46756-46820         c
            1247-1359           AC125183.4         46408-46520         c
            1360-1552           AC125183.4         44831-45023         c
            1553-1690           AC125183.4         40853-40990         c
            1691-1835           AC125183.4         39581-39725         c
            1836-2008           AC125183.4         36527-36699         c
            2009-2146           AC125183.4         35739-35876         c
            2147-2282           AC125183.4         35525-35660         c
            2283-2403           AC125183.4         34358-34478         c
            2404-2474           AC125183.4         32573-32643         c
            2475-3150           AC125183.4         31669-32344         c
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 60.63 cM"
     gene            1..3150
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /note="RAS protein activator like 1 (GAP1 like)"
                     /db_xref="GeneID:19415"
                     /db_xref="MGI:MGI:1330842"
     exon            1..99
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            100..246
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..2581
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /note="isoform 1 is encoded by transcript variant 3;
                     rasGAP-activating-like protein 1; ras
                     GTPase-activating-like protein"
                     /codon_start=1
                     /product="rasGAP-activating-like protein 1 isoform 1"
                     /protein_id="NP_001346073.1"
                     /db_xref="CCDS:CCDS19623.1"
                     /db_xref="GeneID:19415"
                     /db_xref="MGI:MGI:1330842"
                     /translation="MAKSGSLSIRVVEGRALPAKDVSGSSDPYCLVKVDDQVVARTAT
                     IWRSLSPFWGEEYTVHLPLDFHHLAFYVLDEDTVGHDDIIGKISLSKEAITADPRGID
                     SWINLSRVDPDAEVQGEVCLDVKLLEDARGRCLRCHVRQARDLAPRDISGTSDPFARV
                     FWGNHSLETSTIKKTRFPHWDEVLELREAPGTTSPLRVELWDWDMVGKNDFLGMVEFT
                     PQTLQQKPPNGWFRLLPFPRAEDSGGSLGALRLKVRLTEDRVLPSQYYQPLMELLLES
                     VQGPAEEDTTSPLALLEELASGDCRQDLATKLVKLFLGRGLAGPFLDYLTRREVARTN
                     DPNTLFRSNSLASKSMEQFMKLVGMRYLHEVLRPVISRVFEEKKYMELDPCKMDLNRS
                     RRISFKGTPTEEQVRETSLGLLTGYLGSVVDAIVSSTGRCPLALRLAFKQLQRCVEKR
                     FSGIEHQDVKYLAISGFLFLRFFAPAILTPKLFDLRDQHADPQTSRSLLLLAKAVQSI
                     GNLGQQLGQGKEQWLAPLHPFLLQSISRVRDFLDQLVDVDEDEEAGGPACALVQPSTI
                     VREGFLLKRKEEPGGLATRFAFKKRYFRLSGRDLSYSKTPEWQVHTSIPLSCIRAVEH
                     VDEGAFQLPHVMQVVTQDGAGTSHTTYLQCKNVNDLNQWLSALRKASAPNPGKLVACH
                     PGAFRSGRWTCCLQAERSAAGCSRTHSAITLGDWSDPLDPDAEAQAVYRQLLLGRDQL
                     RLKLLEDSSLDTEVDPGRDSSATDGPCAEVLAQQRAATTHLLQVLEDLEQAHEEFQKR
                     G"
     exon            247..303
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            304..417
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            418..479
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            480..609
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            610..691
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            692..823
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            824..909
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1027
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1181
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1246
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1359
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1552
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1690
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1835
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2008
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2146
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2282
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2403
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2474
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..3150
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3125..3130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /note="hexamer: TATAAA"
     polyA_site      3150
                     /gene="Rasal1"
                     /gene_synonym="MRASAL"
                     /note="major polyA site"
ORIGIN      
        1 aattggagac gaagagaaga tgcaagggga tgttcgaggt ccctccctcc actcgcatca
       61 gacccccagg gagcgtgcgc cgcctgccgc ccagagagac tgatgacatg tagggtcacc
      121 tcctggtcca gcctcagaac ctcggcagac tccttgcctg gaaagtttgg gactgtgcga
      181 catggccaag agcggctcgc tgagtatccg cgtggtggag ggacgagcac tgcccgccaa
      241 ggacgtgtct ggaagcagtg acccctattg tctggtgaaa gtggatgacc aagtggtggc
      301 caggacagca accatctgga ggagcctgag ccccttttgg ggggaggagt acaccgttca
      361 ccttccattg gacttccacc acctggcctt ctacgtgctg gatgaggaca ccgttggaca
      421 cgatgacatc attgggaaga tctcattgag caaagaggcg atcacagccg accctcgagg
      481 gatcgacagc tggatcaacc tgagccgagt ggatccagac gctgaagtac agggtgaggt
      541 ctgcctggat gtgaagctat tggaggatgc tcggggccgc tgcctccgct gccacgtgag
      601 acaggccagg gacctggccc cccgggacat ctctggcaca tcggacccat ttgcccgtgt
      661 gttctggggc aaccatagtt tggaaacttc gaccatcaag aagacccgct ttccacactg
      721 ggatgaggtg ttggagctgc gggaggctcc ggggaccacg tccccgctgc gagtggaact
      781 ctgggattgg gacatggtgg gcaagaatga cttcctgggt atggtggagt tcaccccaca
      841 gaccctgcag cagaagccac ctaatggctg gttccggctc ctgccctttc ctagagctga
      901 ggattctggg gggagcctgg gtgccctgcg gctgaaggtg cgcctcactg aggaccgggt
      961 cctgccttcc cagtactacc agcctctcat ggaactgctt ctggagtcgg tgcaagggcc
     1021 ggctgaggag gacaccacca gccccctggc tctgctagag gagctggcct ctggggactg
     1081 tcgtcaggac cttgccacca agctggtgaa gctgttcctg ggccggggcc tggccgggcc
     1141 ctttctagat tatctcacaa ggcgtgaggt ggctcgaacc aatgacccca acaccctctt
     1201 ccgttctaac tccttggcat ccaagtcgat ggaacagttc atgaagctcg tgggcatgcg
     1261 ctacctgcac gaggtcctga ggccagtgat cagccgcgtc ttcgaggaga agaaatatat
     1321 ggaactggac ccttgcaaga tggacctgaa ccgctctcgg aggatctcct tcaagggcac
     1381 gcccacagag gagcaggtac gggagaccag cctggggctg ctgaccggat acctagggtc
     1441 cgttgtggac gccatcgtga gctctacagg gcgctgccca cttgccttgc gcttggcctt
     1501 taagcagctc cagcggtgtg tggagaagcg cttctctggg atagagcatc aggatgtgaa
     1561 gtacctggcc atcagtggct tcctctttct gcggttcttt gcgcctgcca tcctcacacc
     1621 gaaactgttt gacctcagag accagcacgc agacccccag accagccgtt ccctgctgct
     1681 gctcgccaag gctgtgcaga gcattgggaa cctgggccag cagctgggcc agggcaagga
     1741 gcagtggcta gccccgctcc atcccttcct gctgcagagc atctcacgtg tgagggactt
     1801 cctggaccag ctggtggatg tggacgagga tgaggaggcc gggggtccag cctgcgccct
     1861 ggtccaaccc tcaaccattg ttcgagaagg cttcctgctg aagcgcaagg aggagcctgg
     1921 aggcctggcc acgcgctttg ccttcaagaa gcgctacttc cggctgagtg ggcgggacct
     1981 ctcctactcc aagactccgg agtggcaggt tcacacatcc atcccgctgt cctgcatccg
     2041 ggctgtggag catgtggacg agggtgcctt tcaactgcca cacgtcatgc aggtggtgac
     2101 acaggatggc gccgggacgt cacacaccac ctacctccag tgtaagaacg tgaatgacct
     2161 caaccaatgg ctgtcagccc tgcgcaaagc cagtgccccc aacccgggca agctggttgc
     2221 ctgccaccct ggtgccttcc gcagcgggcg ctggacctgc tgcctccagg ctgagcgctc
     2281 agctgctggt tgtagccgca cacactcagc catcacactg ggagactgga gtgacccact
     2341 ggatcctgac gccgaggccc aggcagtgta tcgccagctg ctcctggggc gggaccagct
     2401 caggctgaaa ctgctggagg attccagcct ggacacagaa gtggaccctg ggagggactc
     2461 cagtgccaca gacgggccct gtgctgaggt tctggcccag cagagagcag ccacaaccca
     2521 cctgctgcag gtacttgaag acttggagca agcccacgag gagttccaga aacggggata
     2581 ggagcaagtg gccctctagg atgctggatg ctgagaaagt gccagagcca gctggggaca
     2641 gagccatggg gcctccctga gcattctttg gcccaggcat cttcttgagg cccccttctt
     2701 cactgcttgg ggcctcccag acaaagcctg gtcctgtgtg ctctgtagcc agccagggca
     2761 taggagcctt tggtagggcc cagtcttgta ctgaccaaac ctcactttct tctctgagcc
     2821 tggagtctca gcttcactgg acacagccct tccaataccc gtggcgccca tctcacccca
     2881 agctgctgga tacagctgta cctgaacgcc aaccctggcg gactcagaaa agcattcaat
     2941 aggcttggcc aggaacttcc tgttttccac ttctgctagc cactgtgacc tcacagatcc
     3001 ttctgtattc cactctcatc tggcagcccc aaccaggtgt tctcagaaga acggactggg
     3061 ggcccagcat gccaattcag agcctcgaag ccggacacag gctcttcctc tctcttatac
     3121 cctgtataaa gtgatgcgtg agcatgctta
//
