LOCUS       NM_009025               4118 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus RAS p21 protein activator 3 (Rasa3), mRNA.
ACCESSION   NM_009025 NM_174859
VERSION     NM_009025.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Horitani,S., Ueda,Y., Kamioka,Y., Kondo,N., Ikeda,Y., Naganuma,M.
            and Kinashi,T.
  TITLE     The critical role of Rap1-GAPs Rasa3 and Sipa1 in T cells for
            pulmonary transit and egress from the lymph nodes
  JOURNAL   Front Immunol 14, 1234747 (2023)
   PUBMED   37545505
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4118)
  AUTHORS   Kamioka,Y., Ueda,Y., Kondo,N., Tokuhiro,K., Ikeda,Y., Bergmeier,W.
            and Kinashi,T.
  TITLE     Distinct bidirectional regulation of LFA1 and alpha4beta7 by Rap1
            and integrin adaptors in T cells under shear flow
  JOURNAL   Cell Rep 42 (6), 112580 (2023)
   PUBMED   37267105
REFERENCE   4  (bases 1 to 4118)
  AUTHORS   Johansen,K.H., Golec,D.P., Huang,B., Park,C., Thomsen,J.H.,
            Preite,S., Cannons,J.L., Garcon,F., Schrom,E.C., Courreges,C.J.F.,
            Veres,T.Z., Harrison,J., Nus,M., Phelan,J.D., Bergmeier,W.,
            Kehrl,J.H., Okkenhaug,K. and Schwartzberg,P.L.
  TITLE     A CRISPR screen targeting PI3K effectors identifies RASA3 as a
            negative regulator of LFA-1-mediated adhesion in T cells
  JOURNAL   Sci Signal 15 (743), eabl9169 (2022)
   PUBMED   35857633
REFERENCE   5  (bases 1 to 4118)
  AUTHORS   Robledo,R.F., Ciciotte,S.L., Graber,J.H., Zhao,Y., Lambert,A.J.,
            Gwynn,B., Maki,N.J., Brindley,E.C., Hartman,E., Blanc,L. and
            Peters,L.L.
  TITLE     Differential effects of RASA3 mutations on hematopoiesis are
            profoundly influenced by genetic background and molecular variant
  JOURNAL   PLoS Genet 16 (12), e1008857 (2020)
   PUBMED   33370780
  REMARK    GeneRIF: Differential effects of RASA3 mutations on hematopoiesis
            are profoundly influenced by genetic background and molecular
            variant.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4118)
  AUTHORS   Encinas,J.A., Lees,M.B., Sobel,R.A., Symonowicz,C., Weiner,H.L.,
            Seidman,C.E., Seidman,J.G. and Kuchroo,V.K.
  TITLE     Identification of genetic loci associated with paralysis,
            inflammation and weight loss in mouse experimental autoimmune
            encephalomyelitis
  JOURNAL   Int Immunol 13 (3), 257-264 (2001)
   PUBMED   11222494
REFERENCE   7  (bases 1 to 4118)
  AUTHORS   Ida,A., Hirose,S., Hamano,Y., Kodera,S., Jiang,Y., Abe,M.,
            Zhang,D., Nishimura,H. and Shirai,T.
  TITLE     Multigenic control of lupus-associated antiphospholipid syndrome in
            a model of (NZW x BXSB) F1 mice
  JOURNAL   Eur J Immunol 28 (9), 2694-2703 (1998)
   PUBMED   9754557
REFERENCE   8  (bases 1 to 4118)
  AUTHORS   Baba,H., Fuss,B., Urano,J., Poullet,P., Watson,J.B., Tamanoi,F. and
            Macklin,W.B.
  TITLE     GapIII, a new brain-enriched member of the GTPase-activating
            protein family
  JOURNAL   J Neurosci Res 41 (6), 846-858 (1995)
   PUBMED   7500386
REFERENCE   9  (bases 1 to 4118)
  AUTHORS   Peters,L.L. and Barker,J.E.
  TITLE     Novel inheritance of the murine severe combined anemia and
            thrombocytopenia (Scat) phenotype
  JOURNAL   Cell 74 (1), 135-142 (1993)
   PUBMED   8334700
REFERENCE   10 (bases 1 to 4118)
  AUTHORS   Peters,L.L., McFarland-Starr,E.C., Wood,B.G. and Barker,J.E.
  TITLE     Heritable severe combined anemia and thrombocytopenia in the mouse:
            description of the disease and successful therapy
  JOURNAL   Blood 76 (4), 745-754 (1990)
   PUBMED   2383655
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CA749631.1 and BC057300.1.
            
            On Dec 30, 2006 this sequence version replaced NM_009025.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057300.1, AK141511.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 CA749631.1         13-21
            10-4118             BC057300.1         1-4109
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 6.29 cM"
     gene            1..4118
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /note="RAS p21 protein activator 3"
                     /db_xref="GeneID:19414"
                     /db_xref="MGI:MGI:1197013"
     exon            1..195
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..2645
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /note="GAP1(IP4BP); ins P4-binding protein; R-Ras gap;
                     GAPIII activator 3; Ras GTPase-activating protein III;
                     R-ras RTPase activating protein-3"
                     /codon_start=1
                     /product="ras GTPase-activating protein 3"
                     /protein_id="NP_033051.2"
                     /db_xref="CCDS:CCDS40234.1"
                     /db_xref="GeneID:19414"
                     /db_xref="MGI:MGI:1197013"
                     /translation="MAVEEEGLRVFQSVRIKIGEAKNLPSYPGPNKMRDCYCTVNLDQ
                     EEVFRTKIVEKSLCPFYGEDFYCEIPRSFRHLSFYIFDRDVFRRDSIIGKVAIQKEDL
                     QRYHNRDTWFQLQHVDADSEVQGKVHLELRLSEVITDTGVVCHKLAARIFECQGLPIV
                     NGQCDPYATVTLAGPFRSEAKKTKVKKKTNNPQFDEVFYFEVTRPCSYSKKSHFDFEE
                     EDVDKLEIRVDLWNASNLKFGDEFLGELRLPLKILRHSSSYEAWYFLQPRDNGNKSLK
                     PDDLGSLRLNVVYTEDHVFSSEYYSPLRDLLLKSADVEPVSASAAHILGEVCRDKQEA
                     AIPLVRLLLHYGRVVPFISAIASAEVKRTQDPNTIFRGNSLTSKCIDETMKLAGMHYL
                     HVTLKPTIEEICQSHKSCEIDPVKLKDGENLENNMESLRQYVDRIFTVITKSGVSCPT
                     VMCDIFFSLREAAAKRFQDDLDVRYTAVSSFIFLRFFAPAILSPNLFQLTPHHTDPQT
                     SRTLTLISKTIQTLGSLSKSKSASFKESYMATFYEFFNEQKYADAVKNFLDLISSSGR
                     RDPKSIEQPILLKEGFMIKRAQGRKRFGMKNFKKRWFRLTNHEFTYQKSKGDQPLCNI
                     PIENILAVERLEEESFRMKNMFQVIQPERALYIQANNCVEAKDWIDILTKVSQCNQKR
                     LTVFHPSAYLNGHWLCCRASSDTAAGCTPCTGGLPANIQLDIDGDRETERIYSLFNLY
                     MGKLEKMQEACGSKSVYDGPEQEEYSTFVIDDPQETYKTLKQVIAGVGTLEQEHAQYR
                     RDKFKKTRYGSQEHPIGDKSFQNYIRQQSEISTHSI"
     exon            196..313
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            314..417
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            418..512
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            513..589
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            590..670
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            671..743
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            744..820
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            821..925
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1082
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1231
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1346
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1421
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1545
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1652
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1730
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1808
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1882
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1989
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2072
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2281
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2385
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2569
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..4118
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4096..4101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /note="hexamer: ATTAAA"
     polyA_site      4118
                     /gene="Rasa3"
                     /gene_synonym="E130011G04; GAPIII; hlb381; scat"
                     /note="major polyA site"
ORIGIN      
        1 ggtcagagcg cggaccgcag cgcgcggggc tctgggggca tccgcagctc ggctccgtct
       61 gccggcttct tcccctgtct tgtccccctt ctgcggccgc gctgccgggc cctcgcgcgt
      121 tggcgaggtc gacaagcagc atggcggtgg aggaagaggg actgcgggtc ttccagagcg
      181 tgaggatcaa gattggtgaa gccaagaatc ttccttccta cccggggcca aacaagatga
      241 gggactgcta ctgcactgtg aacctggacc aggaggaggt tttccggacc aaaattgtcg
      301 agaagtcact ctgccccttt tacggagagg acttttactg tgaaatccct cggagtttcc
      361 gccacctgtc cttttacatt tttgatagag atgttttccg aagggactcc atcataggca
      421 aggtggccat ccagaaggaa gacttgcaga ggtaccacaa cagggacaca tggttccagc
      481 tgcagcacgt ggacgcagac tcagaggtgc agggcaaggt ccacctggag ctgagactaa
      541 gtgaggtcat tacggacact ggtgtcgtct gccacaaact cgctgcacgc atcttcgagt
      601 gccagggtct ccccattgtg aacgggcagt gtgaccctta cgccacagtg acgctggccg
      661 gacccttcag gtctgaagca aagaaaacga aggtgaagaa gaaaaccaac aacccccagt
      721 ttgacgaggt gttttatttt gaggtgacca gaccctgcag ctacagcaaa aagtcccact
      781 ttgactttga ggaagaggac gtggacaaac ttgaaatccg agttgacctc tggaacgcca
      841 gcaacctgaa gtttggggat gagtttctcg gggaactgag gcttcctctc aagattctgc
      901 gacactcgag ctcttatgaa gcctggtact tcctccagcc ccgagacaac ggcaataaga
      961 gcctgaagcc agatgacttg gggtctctga ggttaaatgt cgtttataca gaagaccatg
     1021 tcttctcctc tgagtactac agcccactgc gtgacctgtt actgaagtct gcagatgtgg
     1081 agcctgtctc agcctcagca gctcacatcc tgggtgaggt gtgcagagac aagcaagagg
     1141 cagccatccc gctggtacgg ctcctgctgc actatggcag ggtagtgccc ttcatcagtg
     1201 ccatcgctag cgcagaggtg aagaggaccc aggaccccaa taccatcttc cgaggaaact
     1261 cactgacatc caagtgcata gatgagacga tgaagctggc aggcatgcac tatctccatg
     1321 tgaccctgaa gcccaccatt gaggagatct gccagagcca caagtcctgt gagatcgacc
     1381 ctgtgaaact gaaagacggc gaaaacctcg agaacaacat ggagagcctg aggcagtatg
     1441 tggatcgcat cttcactgtc atcaccaagt ccggggtgag ctgccccacc gtcatgtgtg
     1501 acatcttttt ctccttgcgg gaggcggctg ccaagcgctt ccaagatgac ttggatgtga
     1561 ggtacacggc cgtgagcagc ttcatcttcc tcaggttctt cgcccctgcc atcctgtccc
     1621 caaacctttt ccagctgaca ccccaccaca cggatccaca gacttctaga accctgacac
     1681 ttatctcaaa gacgatccag accctcggca gcttgtccaa gtccaagtct gccagtttta
     1741 aggagtcgta catggcgaca ttctatgaat tcttcaatga gcagaagtat gcagatgctg
     1801 taaaaaattt tctggatttg atctcatcct cggggagaag ggaccccaag agcatagagc
     1861 agcccatcct gcttaaagaa gggttcatga tcaagcgggc ccagggaagg aaacggtttg
     1921 gaatgaagaa tttcaagaag aggtggtttc gcctgacgaa ccacgagttc acctaccaga
     1981 aaagcaaagg tgatcagcca ctctgcaaca tccccatcga gaacatcttg gctgtggaga
     2041 ggctagagga ggagtccttc cgaatgaaaa acatgttcca ggtcatccag ccagagcgtg
     2101 ccctgtacat ccaggccaac aactgtgtgg aggccaagga ctggatcgac atcctcacca
     2161 aagtgagcca gtgcaaccag aagcggctca ccgtcttcca cccgtcggcc tacctgaacg
     2221 gccactggct ctgctgcagg gcctcctcag acacggctgc tggctgcact ccctgcactg
     2281 gtgggctccc ggccaacatc cagctggaca ttgatgggga ccgtgaaaca gagcgcatct
     2341 actctctctt taacctgtac atgggcaagc tggaaaagat gcaggaggcc tgcggcagca
     2401 agtctgtgta tgacggccct gagcaggagg agtactcaac gttcgtcatc gacgaccccc
     2461 aggagacgta caagacgctg aagcaggtca tcgctggggt ggggaccctt gagcaggagc
     2521 acgcacagta caggagagac aagttcaaga agacgagata cgggagccag gagcacccta
     2581 ttggagacaa gagcttccag aactacatcc ggcagcagtc tgagatctcc acccattcca
     2641 tttaaggtca cagcacctcc cagatcagcg ctgttgaccc acaccagggg gaaaaagcag
     2701 ataaaagaag gcagaaggca tacatggaga ggaaacatgt tcacccgttc acacgggtcc
     2761 taccaagctg gtggggccac cacgagtcaa gcatgctctt ccccggcagg aactgttgcc
     2821 tgggctgtgc actcccctta gctgccagca caggtggtct gagccatcct ggaatgtatg
     2881 ctcatctgtc tttctactcc ttcagttgta tggggatcca gggatctgtt aaatattagg
     2941 gaagtatgga ctttgtctaa acctgttccc tcgcagcccg gtcccccttc acaaaggcac
     3001 gcctggttcg ttcatgccag ggcgcacctt cttcagtgtg cactcttgtc ccttgcctgc
     3061 tggtctctgt ggctccgtac acatgtagca gtctggccct agccttagac gtacacgcaa
     3121 tcctgccgtt cgttccacgg aatccctgtc tgaaacgtga gaagcagtat tccctggcag
     3181 ccagcaggca ggcagccttt ctcctgagct tatgttggtt tgttcctggc taaagggtca
     3241 tttttgtggc tgggatggac aggatggtac agaaactcct ttatcttaga ggacatgggg
     3301 gtccttttct tagagtccct ggagagggag ggctgtgagc ccagtcttct ctgggtgacc
     3361 atcacccctc agttcaaaca caggtctgga tgttggataa ggtttcaaac tagaatccac
     3421 aggcttgctg cctcgatgaa agtctgggaa agatttttaa aggctgtgca tttttcaagc
     3481 tgtctttctt aatgttttta aaggactgtt tttaaatagc attttgatat aaggcagtgt
     3541 ggaataccag catctatgtg cattaggggg gtgacccagg gaaatccaaa gggaacagta
     3601 tttgatttct cacaaagcca aagggtcctc ggggacatag ctctgagatg gagcctgcac
     3661 cggcagcaat aaaggccaaa cgtggtattc cacatgcctg tgtctctgtg agcccaacag
     3721 gccacacatg ccaatgtctg gaaccacttt tgattggggt gggagggtcc tgaaacagac
     3781 aagacagact gtaccagctt agcccccaca gtactgctgg agccttatgc caaactgcat
     3841 ttccagaggc tgagagaggg gcactctatg ggcagatgtt gctgaaggac acaaccacac
     3901 attctggtac ccttttctct tcattcttgt tgggaggttt aatctgtatc tagaacccat
     3961 agtagcagag gatggaaaca aaagtcagtt tggacacagc aggccccttg tactgaactt
     4021 ctttttaact tggatttgta acttaatgtt tctgtttata agatactaac gatttgcaat
     4081 gtattttaat gggcaattaa agcagatgtt ttattctt
//
