LOCUS       NM_181406               1881 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus arginyl-tRNA synthetase 2, mitochondrial (Rars2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_181406
VERSION     NM_181406.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928738.8, AK075721.1, AK166986.1 and AW120679.1.
            
            On Oct 10, 2017 this sequence version replaced NM_181406.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156182.1, BC024878.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AL928738.8         10459-10475
            18-331              AK075721.1         2-315
            332-1069            AK166986.1         847-1584
            1070-1245           AK075721.1         1050-1225
            1246-1684           AK166986.1         1757-2195
            1685-1881           AW120679.1         1-197               c
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 16.84 cM"
     gene            1..1881
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /note="arginyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:109093"
                     /db_xref="MGI:MGI:1923596"
     exon            1..70
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1771
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /EC_number="6.1.1.19"
                     /note="probable arginyl-tRNA synthetase, mitochondrial;
                     argRS; arginine--tRNA ligase; arginyl-tRNA synthetase 2,
                     mitochondrial (putative); probable arginine--tRNA ligase,
                     mitochondrial"
                     /codon_start=1
                     /product="probable arginine--tRNA ligase, mitochondrial
                     precursor"
                     /protein_id="NP_852071.2"
                     /db_xref="CCDS:CCDS18029.1"
                     /db_xref="GeneID:109093"
                     /db_xref="MGI:MGI:1923596"
                     /translation="MACGFRRSIACQLSRVLALPPESLIKSISAVPVSKKEEVADFQL
                     SVDSLLEDNNHKSQVDTQDQARRLAEKLKCDTVVTAISAGPRTLNFKINRELLTKAVL
                     QQVTEDGCKYGLKSELFSDLPKKRIVVEFSSPNIAKKFHVGHLRSTIIGNFIANLKEA
                     LGHQVTRINYIGDWGMQFGLLGTGFQLFGYEEKLQTNPLQHLFDVYVQVNKEATDDKN
                     VTKLAHEFFHRLEMGDTQALSLWQRFRDLSIEEYTQIYKRLGIYFDEYSGESFYREKS
                     QDVLKLLDSKGLLQKTAEGNVVVDLSGTGDLSSVCTVMRSDGTSLYATRDLAAAIHRM
                     DKYNFDTMIYVADKGQRRHFQQVFQMLKIMGYDWAERCQHVPFGIVKGMKTRRGGVTF
                     LEDVLNEVQSRMLQNMASIKTTKQLENPQETAERVGLAAVIIQDFRGTLLSDYQFSWD
                     RVFQSRGDTGVFLQYTHARLCSLEETFGCGYLNDSNVACLQEPQSVSILQHLLRFDEV
                     LYLSSQDLQPKHIVSYLLTLSHLAAVAHKTLQVKDSPPDVAGARLHLFKAVRSVLANG
                     MKLLGITPVCRM"
     exon            71..144
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            145..247
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            248..331
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            332..429
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            430..485
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            486..569
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            570..646
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            647..805
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            806..912
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1008
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1069
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1146
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1271
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1339
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1449
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1545
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1620
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1684
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1868
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1837..1842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /note="hexamer: AATAGA"
     polyA_site      1868
                     /gene="Rars2"
                     /gene_synonym="1500002I10Rik; PRO1992; Rarsl"
                     /note="major polyA site"
ORIGIN      
        1 cggcgcggag gcggggcgtg cgggaaaggg aggcatggcg tgtggctttc gtaggtcgat
       61 cgcctgtcag ctttccagag tattggctct tccaccagaa agcttgatca agtcaatatc
      121 tgctgttcca gtttcaaaaa aagaagaggt ggcagatttt cagctttcgg tggattcctt
      181 actagaagac aacaatcaca agtcacaggt agacactcaa gaccaagcca ggagattggc
      241 agagaagctt aagtgtgata cagtggtgac agccatcagt gctggcccaa ggactctaaa
      301 tttcaaaata aacagagaat tattaacaaa ggcagtattg caacaagtta ctgaagatgg
      361 ctgtaaatat gggttaaaaa gtgaactttt ctcagatctt cccaagaaga ggattgtggt
      421 tgaattcagt tcacctaata ttgccaaaaa atttcatgtt ggacatttgc gctccaccat
      481 cataggaaat tttatagcaa atctaaaaga agctttagga catcaagtaa caagaataaa
      541 ttacattgga gattggggca tgcagtttgg tctgctggga actggtttcc agctgtttgg
      601 ctatgaagag aaactccaga ccaatccttt acagcatctc tttgatgttt atgtacaagt
      661 taataaagaa gcaacagatg ataaaaatgt aacaaaattg gcacacgagt tcttccaccg
      721 actagaaatg ggtgacacgc aggcactgtc actgtggcag agattccggg acttgagcat
      781 tgaagagtac acccagattt acaagcgtct tggaatatac tttgatgaat attcaggaga
      841 atcattttat cgtgaaaaat ctcaagatgt cttaaagttg cttgacagca aaggacttct
      901 gcagaaaaca gcggagggaa atgttgtagt agatctctct gggactggtg acctctcttc
      961 tgtctgcact gtgatgcgaa gtgatgggac ctctctctat gccaccaggg accttgcagc
     1021 tgctatacat cgcatggaca agtacaattt tgacacaatg atttatgtcg cagacaaagg
     1081 gcaaagaagg cattttcagc aagtgttcca aatgctgaag atcatgggct acgactgggc
     1141 ggaaaggtgc cagcatgtcc cctttgggat agtaaagggg atgaagactc gaagaggagg
     1201 ggtcactttt ctggaagatg tcttaaatga ggttcaatca aggatgctac agaacatggc
     1261 ttccattaag accacgaaac aattggagaa ccctcaggag acggcagaga gagtggggct
     1321 tgccgccgtc atcatccagg acttcagagg cactctctta tctgactatc agttcagttg
     1381 ggatcgtgtt ttccagagcc gtggggacac tggagtcttc ctgcagtaca cccatgcccg
     1441 cctctgtagt ttggaagaga cttttggatg tgggtatctc aatgactcca atgttgcttg
     1501 tttacaagag ccgcagtctg tttcaattct ccagcatctt ctcaggtttg atgaggtact
     1561 ttacctgtca tctcaggacc tacagccgaa gcacatcgtt agctacctcc taactctaag
     1621 tcaccttgca gctgtggcac ataaaacact ccaggtgaaa gacagtcctc ctgatgtggc
     1681 tggggccaga cttcaccttt tcaaagctgt ccgttctgtc ttagccaatg gaatgaaact
     1741 tcttggaatt acacctgtat gtagaatgta atttctagat aaatatgttt tttatgcaaa
     1801 tgagttctag ttgttatcag ataccttgct gttcagaata gaatttaaag tattattctg
     1861 tcccagaaaa aaaaaaaaaa a
//
